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36 2 Apoptosis 2.5 Annexin V Detection Kits Applications be tagged with EGFP (Enhanced Green in the provided binding buffer, apoptotic Fluorescent Protein). Since Annexin V cells show red fluorescence, necrotic cells Screening for apoptotic cells conjugates and tagged annexins retain show green fluorescence, and live cells Differentiating apoptotic and their high affinity for PS, they are ide- show little or no fluorescence. These necrotic cells ally suited as highly sensitive probes to populations can easily be distinguished Annexin detection identify early apoptotic cells and to de- by fluorescence microscopy and flow cy- Flow cytometry tect apoptosis much earlier than assays tometry using the appropriate filters and Fluorescence microscopy based on DNA fragmentation or loss of channels. membrane integrity. The stained cells Features can be evaluated by flow cytometry or Individual Annexin V Reagents fluorescence microscopy using common High fluorescence intensity and filter sets and flow cytometer channels. PromoKine also offers a wide range of 2 Apoptosis sensitivity Many different labels and tags available Annexin V Detection Kits Annexin V reagents separately. For easy staining of early apoptotic cells, Annexin V conjugated to FITC, PE, Cy3, Appropriate for various multiwell The Annexin V Apoptosis Detection Cy5, or biotin is available, as well as An- formats and automation Kits include an Annexin V conjugate or nexin V tagged with EGFP. The stained Excitation and emission spectra are tagged Annexin V as well as binding buf- cells can be evaluated by flow cytometry compatible with common excitation fer and in some cases Propidium Iodide or fluorescence microscopy using com- sources and filter sets (PI) for differentiating between early mon filter sets and flow cytometer chan- apoptotic and dead cells. The kits allow nels. Biotinylated Annexin V can be de- In the early stages of apoptosis, changes a one-step staining procedure that takes tected in conjunction with conventional can be observed on the cell surface. only 10 minutes. In addition, the assay dye-staining using any streptavidin- or <strong>Cell</strong>s lose their phospholipid membrane can be performed directly on living cells. avidin-dye reagents such as PromoFluor- asymmetry and expose phosphatidyl- streptavidin/avidin conjugates, fluores- serine (PS) on the exterior plasma mem- Promokine Annexin V Apoptosis Detec- cein-, PE-, or APC-streptavidin as well brane. Annexin V is a Ca 2+ -dependent, tion Kit plus includes an additional dye, SY- as streptavidin/avidin conjugated to phospholipid-binding protein with high TOX green, that is impermeant to living peroxidase, alkaline phosphatase (AP), affinity for PS and is very useful for and apoptotic cells, but stains necrotic b-galactosidase, etc. identifying apoptotic cells that expose cells with intense green fluorescence PS on their surfaces to the extracellular by binding to cellular nucleic acids. Af- Please visit www.promokine.info or environment. Annexin V may be conju- ter staining a cell population with e.g., see the PromoKine Price List to find our gated to biotin or fluorophores such as fluorescent Annexin V-Cy3 or Annexin complete range of individual Annexin V FITC and phycoerythrin (PE). It can also V-PE conjugates, and SYTOX Green dye reagents. Product Size Catalog Number Annexin V-FITC Detection Kit Annexin V-FITC Detection Kit Plus Annexin V-Cy3 Detection Kit Annexin V-Cy3 Detection Kit Plus Annexin V-Cy5 Detection Kit Annexin V-EGFP Detection Kit For prices please see our online shop at www.promokine.info 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays PK-CA577-K101-25 PK-CA577-K101-100 PK-CA577-K101-400 PK-CA577-K201-25 PK-CA577-K201-100 PK-CA577-K201-400 PK-CA577-K102-25 PK-CA577-K102-100 PK-CA577-K102-400 PK-CA577-K202-25 PK-CA577-K202-100 PK-CA577-K202-400 PK-CA577-K103-25 PK-CA577-K103-100 PK-CA577-K103-400 PK-CA577-K104-25 PK-CA577-K104-100 PK-CA577-K104-400
2 Apoptosis 37 Product Size Catalog Number Annexin V-PE Detection Kit Annexin V-PE Detection Kit Plus Annexin V-PE-Cy5 Detection Kit Annexin V-Biotin Detection Kit Annexin V-FITC Reagent Annexin V-Cy3 Reagent Annexin V-Cy5 Reagent Annexin V-EGFP Reagent Annexin V-PE Reagent Annexin V-PE-Cy5 Reagent Annexin V-Biotin Reagent 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 200 assays 1000 assays 200 assays 1,000 assays 200 assays 1,000 assays 200 assays 1,000 assays 200 assays 1,000 assays 200 assays 1,000 assays 100 μg 1 mg PK-CA577-K128-25 PK-CA577-K128-100 PK-CA577-K128-400 PK-CA577-K203-25 PK-CA577-K203-100 PK-CA577-K203-400 PK-CA577-K129-25 PK-CA577-K129-100 PK-CA577-K129-400 PK-CA577-K109-25 PK-CA577-K109-100 PK-CA577-K109-400 PK-CA577-1001-200 PK-CA577-1001-1000 PK-CA577-1002-200 PK-CA577-1002-1000 PK-CA577-1013-200 PK-CA577-1013-1000 PK-CA577-1004-200 PK-CA577-1004-1000 PK-CA577-1014-200 PK-CA577-1014-1000 PK-CA577-1015-200 PK-CA577-1015-1000 PK-CA577-1003-100 PK-CA577-1003-1000 Annexin V Unlabeled Reagent 100 μg PK-CA577-1005-100 1X Annexin V Binding Buffer 100 ml PK-CA577-1035 10X Annexin V Binding Buffer 100 ml PK-CA577-1006 2 Apoptosis For prices please see our online shop at www.promokine.info
Page 1 and 2: Cell Biology Products 2011 | 2012
Page 3 and 4: Overview 1 Overview 1 Cell Analysis
Page 5: Contents 3 4 5 6 7 8 Gene Cloning,
Page 8 and 9: 6 Contents Chapter 1 1 Cell Analysi
Page 10 and 11: 8 1 Cell Analysis Bioluminescent Ce
Page 12 and 13: 10 1 Cell Analysis 1.3 Cytotoxicity
Page 14 and 15: 12 1 Cell Analysis Product Size Cat
Page 16 and 17: 14 1 Cell Analysis Product Product
Page 26 and 27: 24 1 Cell Analysis 1.9 Cell Stress
Page 28 and 29: 26 1 Cell Analysis 1.10 Cell Fracti
Page 30 and 31: 28 1 Cell Analysis 1.11 Nucleic Aci
Page 33 and 34: Apoptosis 2 Apoptosis, or programme
Page 35 and 36: 2 Apoptosis 33 2.1 DNA Fragmentatio
Page 37: 2 Apoptosis 35 2.4 Glutathione Dete
Page 41 and 42: 2 Apoptosis 39 Caspase Screening Ki
Page 43 and 44: 2 Apoptosis 41 Product Size Catalog
Page 45 and 46: 2 Apoptosis 43 2.8 Kinase Detection
Page 47 and 48: 2 Apoptosis 45 2.10 Apoptotic Cell
Page 49 and 50: Fluorescent Labeling 3 The availabi
Page 51 and 52: 3 Fluorescent Labeling 49 3.1 Promo
Page 53 and 54: 3 Fluorescent Labeling 51 Excitatio
Page 55 and 56: 3 Fluorescent Labeling 53 3.2.2 Pro
Page 57 and 58: 3 Fluorescent Labeling 55 3.3.3 Pro
Page 59 and 60: 3 Fluorescent Labeling 57 3.4 Phyco
Page 61 and 62: 3 Fluorescent Labeling 59 3.5 Phyco
Page 63 and 64: Gene Cloning, Expression, 4 and Sil
Page 65 and 66: 4 Gene Cloning, Expression, and Sil
Page 73: 4 Gene Cloning, Expression, and Sil
Page 76 and 77: 74 Contents Chapter 5 5 Cell Transf
Page 78 and 79: 76 5 Cell Transfection Fig. 4: Comp
Page 80 and 81: 78 5 Cell Transfection n Some cell
Page 82 and 83: 80 5 Cell Transfection Phase contra
Page 84 and 85: 82 5 Cell Transfection Magnet Plate
Page 87 and 88: Antibodies and ELISAs 6 Antibodies
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6 Antibodies and ELISAs 87 6.1 Mono
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6 Antibodies and ELISAs 89 n Kinase
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6 Antibodies and ELISAs 91 6.2 Labe
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6 Antibodies and ELISAs 93 6.3 ELIS
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6 Antibodies and ELISAs 95 Product
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Cytokines and Growth 7 Factors Cyto
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7 Cytokines and Growth Factors 109
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138 Contents Chapter 8 8 Microbial
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140 8 Microbial Detection and Elimi
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142 8 Microbial Detection and Elimi
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144 Alphabetical Index Alphabetical
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146 Alphabetical Index Description
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160 Alphabetical International Inde
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162 International Alphabetical Inde
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Notes Notes 165
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Terms and Conditions 167 4. The rig
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Fax Order Form Shipping Address Inv
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