Cell Biology Products

Cell Biology Products 

2011 | 2012

PromoKine 

a brand of PromoCell 

Your Cell Research Partner 

www.promokine.info 

The Cell Culture Experts 

www.promocell.com 

Training Courses 

www.promocell-academy.com 

Please visit our websites regularly for upcoming new products and training courses

Overview 1 

Overview 

1 

Cell Analysis 

2 

Apoptosis 

3 

Fluorescent Labeling 

4 

Gene Cloning, Expression, and Silencing 

5 

Cell Transfection 

6 

Antibodies and ELISAs 

7 

Cytokines and Growth Factors 

8 

Microbial Detection and Elimination

2 

Contents 

Contents 

1 

2 

3 


1.1 Cell Viability and Proliferation Kits ................................. 7 

1.2 Live/Dead Cell Staining Kits. ...................................... 9 

1.3 Cytotoxicity Kits .............................................. 10 

1.4 Senescence Detection Kit. ....................................... 10 

1.5 Cell-Based Reporter Assays ...................................... 11 

1.6 Cell Staining Reagents and Related Products ......................... 13 

1.7 Intracellular Indicators and Related Products ......................... 19 

1.8 Cell Signaling Assays ........................................... 23 

1.9 Cell Stress and Metabolism Kits .................................. 24 

1.10 Cell Fractionation and Protein Extraction Kits ....................... 26 

1.11 Nucleic Acid Isolation Kits ...................................... 28 

Apoptosis 

2.1 DNA Fragmentation Detection Kits ................................ 33 

2.2 Mitochondrial Apoptosis Staining Kit ............................... 34 

2.3 Cytochrome c Apoptosis Detection Kit. ............................. 34 

2.4 Glutathione Detection Kits. ...................................... 35 

2.5 Annexin V Detection Kits ....................................... 36 

2.6 Caspase Kits ................................................. 38 

2.7 Cathepsin/Calpain Detection Kits ................................. 42 

2.8 Kinase Detection Kits .......................................... 43 

2.9 Apoptotic and Necrotic Cell Detection .............................. 44 

2.10 Apoptotic Cell Isolation Kit ..................................... 45 

2.11 Apoptosis Inducers ........................................... 45 


3.1 PromoFluor Dyes. ............................................. 49 

3.2 PromoFluor Labeling Kits ........................................ 52 

3.2.1 PromoFluor Biomolecules Labeling Kits ............................ 52 

3.2.2 PromoFluor DNA Labeling Kits .................................. 53 

3.3 PromoFluor Labeled Biomolecules . . . .............................. 54 

3.3.1 PromoFluor Labeled Antibodies . . . . . . . .......................... 54 

3.3.2 PromoFluor Labeled Phalloidin . . . . . . . . . . . ....................... 54 

3.3.3 PromoFluor Labeled Nucleotides . .. .............................. 55 

3.3.4 PromoFluor Labeled Biotin and Streptavidin ........................ 56 

3.4 Phycobiliprotein Dyes . . . . . . . ................................... 57 

3.5 Phycobiliprotein Labeled Biomolecules. ............................. 59 

3.6 PromoFluor Antifade Reagent . . . . . . .............................. 60 

For an up to date list of 

available antibodies please see 

www.promokine.info/antibodies

Contents 3 

4 

5 

6 

7 

8 

Gene Cloning, Expression, Silencing 

4.1 Plasmid Vectors ............................................... 63 

4.1.1 pPK-CMV Expression Vectors . ................................. 63 

4.1.2 pPK-CMV Reporter Vectors. ................................... 64 

4.1.3 pPK-CMV Fusion Vectors . .................................... 65 

4.2 StarGate Cloning Systems . ...................................... 66 

4.2.1 StarGate Combinatorial Cloning System . ......................... 66 

4.2.2 StarGate Mutagenesis System . ................................. 67 

4.2.3 StarGate Fusion Cloning System ................................. 68 

4.3 Gene Silencing. ............................................... 70 

4.3.1 GeneOFF shRNA Vector Kits. ................................... 70 

4.3.2 GeneOFF siRNA Generation Kit ................................. 71 


5.1 PromoFectin Transfection Reagents . .............................. 75 

5.2 Magnet Assisted Transfection (MATra) . ............................ 79 

5.3 Transfection Accessories ........................................ 83 


6.1 Mono- and Polyclonal Antibodies ................................. 87 

6.2 Labeled Antibodies and Labeling Kits .. ............................. 91 

6.3 ELISA Kits ................................................... 93 

6.4 Antigens and Cell Lysates ...................................... 106 

Cytokines and Growth Factors 

7.1 Cytokines, Chemokines, Growth Factors, Hormones, and soluble Receptors 109 

7.2 Animal-free Cytokines and Growth Factors ......................... 131 

7.3 CD Antigens ................................................ 134 

7.4 Cytokines and Growth Factors for Stem Cell Research ................. 135 

Microbial Detection and Elimination 

8.1 Mycoplasma Test Kits . . ....................................... 139 

8.2 Mycoplasma Elimination Solutions . . ............................. 140 

8.3 Bacteria Test Kit . ............................................ 142 

8.4 Disinfectants ................................................ 143 

Appendix 

Alphabetical Index .............................................. 144 

International Distributors .......................................... 160 

Terms and Conditions ............................................ 166 

Ordering and Technical Support. .................................... 168


1 

The specific analysis of cellular processes and conditions has 

played an increasingly vital role in cell biology. Monitoring reporter 

gene expression in cells, determining cell viability and 

proliferation, testing for cytotoxicity, and distinguishing live and 

dead cells are important procedures in most cell biology labs. 

Measuring intracellular signaling and assaying distinct cell metabolic 

activities have also become routine. These applications all 

require reliable, sophisticated, and user-friendly research tools.

6 

Contents Chapter 1 

1 


1.1 Cell Viability and Proliferation Kits ....... 7 

1.2 Live/Dead Cell Staining Kits. ............ 9 

1.3 Cytotoxicity Kits .................... 10 

1.4 Senescence Detection Kit. ............. 10 

1.5 Cell-Based Reporter Assays . ........... 11 

1.6 Cell Staining Reagents and 

Related Products ................... 13 

1.7 Intracellular Indicators and 

Related Products ................... 19 

1.8 Cell Signaling Assays ................ 23 

1.9 Cell Stress and Metabolism Kits ........ 24 

1.10 Cell Fractionation and 

Protein Extraction Kits ............... 26 

1.11 Nucleic Acid Isolation Kits . ............ 28

1 Cell Analysis 7 

1.1 Cell Viability and Proliferation Kits 

Applications 

Cell viability and proliferation assays 

Growth factor/cytokine assays 

Optimization of cell culture conditions 

Cell number determination 

Features 

Non-radioactive method 

Easy and fast procedure 

Reliable and reproducible results 

Appropriate for various multiwell 

formats and automation 

PromoKine offers several types of cell 

viability kits: The Fluorometric Cell 

Viability Kits I and II; the Colorimetric 

Cell Viability Kits I, II, III, and IV; and the 

Bioluminescent Cell Viability Kits I and II. 

Because all of the kits are designed for 

use with various multiwell formats, they 

are also suited for automation. 

Viable cell 

Esterase 

Calcein-AM 

R: acetoxymethyl 

Calcein 

Fluorometric Cell Viability Kit I 

(Resazurin) 

The resazurin-based PromoKine Cell 

Viability Kit I provides a simple and rapid 

measurement of cell viability. Moreover, 

this single-step, homogenous cell pro-liferation 

assay is sensitive, safe, and costeffective. 

It only requires as few as 80 

cells for reproducible results. The redox 

dye Resazurin (also known as Alamar 

Blue) detects cell viability by converting 

from a non-fluorescent, blue dye to the 

highly red fluorescent dye resorufin in 

response to chemical reduction of the 

growth medium caused by cell growth. 

The fluorescent or colorimetric signal 

generated from the assay is proportional 

to the number of living cells in the 

sample. The kit reagents are nontoxic to 

cells and allow kinetic monitoring. While 

fluorometric resorufin quantitation is 

more sensitive, colorimetric measurement 

is also possible. 

Fluorometric Cell Viability Kit II 

(Calcein) 

The PromoKine Fluorometric Cell Viability 

Kit II is intended for the reliable 

fluorometric determination of viable cell 

numbers. It utilizes the cell membranepermeable, 

non-fluorescent dye Calcein- 

AM that is hydrolyzed by esterases to the 

cell membrane-impermeable green-fluorescent 

Calcein (Fig. 1). Since esterases 

are only active in viable cells, the amount 

of Calcein-AM hydrolyzed by cellular 

esterases is directly proportional to the 

number of viable cells in culture. The kit 

has a detection range from less than 50 

to more than 25,000 cells per well. 

Colorimetric Cell Viability Kits I 

(WST-8) and II (WST-1) 

The Colorimetric Cell Viability Kits I and 

II allow for easy and reliable colorimetric 

determination of viable cell numbers with 

excellent sensitivity and linearity. The kits 

utilize the tetrazolium salts WST-8 (Kit I) 

and WST-1 (Kit II) that are reduced to 

water-soluble, orange formazan dyes by 

dehydrogenases present in viable cells 

(see Fig. 2). The absorbance of the formazan 

dye is proportional to the number 

of metabolically active cells and can be 

measured directly from a 96-well plate 

without additional processing. 

1 Cell Analysis 

Colorimetric Cell Viability Kits III 

(XTT) and IV (MTT) 

Fig. 1: Assay mechanism of Calcein-AM conversion by intracellular esterases. 

WST-8 

slightly yellow 

Viable cell 

NADH, NADPH 

NAD, NADP 

WST-8 Formazan 

orange dye 

1-Methoxy PMS 

Dehydrogenase 

Fig. 2: Assay mechanism of WST-8 conversion by dehydrogenases in viable cells. 

The PromoKine Colorimetric Cell Viability 

Kits III and IV have been developed 

for determining the number of viable 

cells in culture. They are based on the 

reduction of the tetrazolium salts XTT or 

MTT into colored formazan compounds 

by mitochondrial enzymes. Since these 

enzymes are inactivated shortly after 

cell death, this is a reliable method for 

the detection of viable cells. The absorbance 

of the formazan product can be 

measured directly from a 96-well plate 

without additional processing. 

In addition, PromoKine also provides 

high-quality tetrazolium salts for preparing 

custom cell assays including INT, 

MTT, XTT, and various WST salts such as 

WSt-8. Please visit our website to find 

the complete range of tetrazolium salts.

8 


Bioluminescent Cell Viability Kit I 

(ATP) 

Bioluminescent Cell Viability Kit II 

(ADP/ATP) 

Cell Proliferation Kit I (CFSE) 

PromoKine’s Cell Proliferation Kit I 

The Promokine Bioluminescent Cell 

The ADP/ATP ratio is a good indicator for 

provides a convenient and sensitive 


Viability Kit I employs bioluminescence 

to measure the ATP level as a marker for 

cell viability in mammalian cells. It utilizes 

luciferase to catalyze the ATP dependent, 

cell viability. High ATP and low ADP levels 

are usually found in proliferating cells. In 

contrast, decreased levels of ATP and increased 

levels of ADP are characteristic for 

way to determine cell proliferation or 

cell division. The assay is based on the 

amine-reactive dye CFDA SE (5-(and 

6)-carboxyfluorescein diacetate, succin- 

light-producing oxidation of luciferin. 

apoptotic cells. In necrotic cells, the ADP 

imidyl ester; also known as CFSE) which 

This light formation can be detected us- 

levels are even higher, and ATP can be 

is non-fluorescent and can diffuse pas- 

ing a luminometer or beta counter. 

barely detected. The Bioluminescent Cell 

sively into cells. When it enters the cells, 

The assay can be performed directly in 

Viability Kit II utilizes bioluminescence to 

the acetate group is removed by intracel- 

culture plates requiring no harvesting, 

measure the ADP/ATP ratio providing an 

lular esterases yielding the bright green- 

washing, or sample preparation and can 

innovative system for fast, simultaneous 

fluorescent dye carboxyfluorescein SE. 

be fully automated for high throughput 

screening of cell proliferation, apoptosis, 

Its succinimidyl ester (SE) reactive group 

(10 seconds/sample). It is highly sensi- 

necrosis, and growth arrest in mammalian 

reacts with intracellular amines and at- 

tive, detecting 10-100 mammalian cells 

cells. The ATP level is determined using 

taches the fluorescent dye to intracellular 

per well. It also offers applications be- 

the luciferin-luciferase reaction used in 

proteins, retaining it in the cells and al- 

sides cell viability testing, e.g., detect- 

the Bioluminescent Cell Viability Kit I. The 

lowing fixation with aldehyde fixatives. 

ing low level bacterial contamination in 

ADP level is measured by its conversion 

CFDA SE has been used as a tracer for 

blood, milk, urine, soil, and sludge. 

to ATP, which is subsequently detected 

several cell divisions as the label is stably 

using the same luciferase-catalyzed re- 

inherited by daughter cells through suc- 

action. The assay works with full auto- 

cessive cell divisions or after cell fusion 

mation for high throughput, detects as 

and is not transferred to adjacent cells in 

few as 100 mammalian cells per well, 

a population. 

and offers highly consistent results with 

The kit is compatible with flow cytometry 

excellent correlation to other apoptosis 

and microscopy and contains 10 single- 

markers (e.g., TUNEL-based or caspase 

use vials as well as a detailed protocol for 

assays). 

cellular labeling. 

Product Size Catalog Number 

Fluorometric Cell Viability Kit I (Resazurin) 

500 assays 

2500 assays 

10000 assays 

PK-CA707-30025-0 

PK-CA707-30025-1 

PK-CA707-30025-2 

Fluorometric Cell Viability Kit II (Calcein) 1000 assays PK-CA707-30026 

Colorimetric Cell Viability Kit I (WST-8) 

Colorimetric Cell Viability Kit II (WST-1) 

Colorimetric Cell Viability Kit III (XTT) 

Colorimetric Cell Viability Kit IV (MTT) 

Bioluminescent Cell Viability Kit I (ATP) 

100 assays 

500 assays 

500 assays 

2500 assays 

200 assays 

1000 assays 

200 assays 

1000 assays 

200 assays 

1000 assays 

PK-CA705-CK04-100 

PK-CA705-CK04 

PK-CA577-K301-500 

PK-CA577-K301-2500 

PK-CA20-300-200 

PK-CA20-300-1000 

PK-CA707-30006-200 

PK-CA707-30006 

PK-CA577-K254-200 

PK-CA577-K254-1000 

Bioluminescent Cell Viability Kit II (ADP/ATP) 200 assays PK-CA577-K255 

Cell Proliferation Kit I (CFSE) 10 x 50 µg PK-CA707-30050 

Resazurin, sodium salt 10 mg PK-CA707-10054 

For prices please see our online shop at www.promokine.info 

PromoKine also provides high-quality tetrazolium salts for preparing custom cell assays including INT, MTT, XTT, and various 

WST salts such as WST-8. Please visit our website to find the complete range of tetrazolium salts.


1.2 Live/Dead Cell Staining Kits 

Applications 

Ethidium homodimer III (EthD-III). Cal- 

Bacteria Live/Dead Staining Kit 

cein-AM penetrates the cell membrane 

Monitoring of viable and dead cells 

of living cells. Once inside, the cellular 

The Bacteria Live/Dead Staining Kit en- 

Cell counting by fluorescence micros- 

esterases present in living cells hydrolyze 

ables a two-color fluorescent staining 

copy 

Features 

it to cell membrane-impermeable, greenfluorescent 

Calcein (emission maximum 

at 515 nm). 

In contrast, EthD-III can only pass 

on both live bacteria (green) and dead 

bacteria (red) using the two nucleic 

acid dyes DMAO and EthD-III. DMAO 

is a green-fluorescent dye which stains 


Simultaneous monitoring of viable 

through the disrupted membrane areas 

both live and dead bacteria with intact 

and dead cells 

of dead cells where it intercalates with 

and damaged cell membranes while 

High fluorescent intensity 

the DNA of the nucleus, emitting red 

the red-fluorescing EthD-III only stains 

Fast and convenient procedure 

light at 620 nm. Compared with PI, 

dead bacteria with damaged cell mem- 

Excitation and emission spectra are 

EthD-III is more stable and emits brighter 

branes. With an appropriate mixture of 

compatible with common excitation 

fluorescent signals. Since both Calcein 

both dyes, viable bacteria with intact cell 

sources and filter sets 

and EthD-III can be excited at 490 nm, 

membranes are stained green, whereas 

Live/Dead Cell Staining Kit II 

a simultaneous monitoring of viable and 

dead cells is possible using a fluorescence 

bacteria with damaged cell membranes 

fluoresce red. The kit is applicable to most 

microscope. At an excitation of 545 nm, 

bacteria types for viability and cytotoxic- 

The Live/Dead Cell Staining Kit II utilizes 

the number of dead cells can be moni- 

ity studies, and suitable for fluorescence 

two fluorescent dyes, Calcein-AM and 

tored. 

microscopy and flow cytometry. 

Calcein-AM 

R: acetoxymethyl 

EthD-III 

Esterase 

Viable cell 

DNA intercalation 

red fluorescence 

Fig. 4: Double-staining of viable 

Calcein 

green fluorescence 

(green) and dead (red) cells using the 

PromoKine Live/Dead Cell Staining 

Kit II. 

Fig. 3: Assay mechanism of the PromoKine Live/Dead Cell Staining Kit II. 


Live/Dead Cell Staining Kit II 300-1000 assays PK-CA707-30002 

Bacteria Live/Dead Staining Kit 100-1000 assays PK-CA707-30027 


Please see also the PromoKine Apoptotic/Necrotic/Healthy Cells Detection Kit in Chapter 2 and our individual stains for viable 

and dead cells in Chapter 1.6.

10 


1.3 Cytotoxicity Kits 


Applications 

Cytotoxicity assays 

Chemosensitivity testing 

Toxicity screening 

Features 


Highly sensitive 


Compatible with common plate readers 

LDH Cytotoxicity Kit II 

The colorimetric LDH Cytotoxicity Kit 

II provides a fast, sensitive, and precise 

alternative to the radioactive [ 3 H]-thymidine 

and [ 51 Cr] release assays. It is based 

on the activity of lactate dehydrogenase 

(LDH), a stable cytoplasmic enzyme that 

is present in all cells. Damaged cells 

rapidly release this enzyme into the surrounding 

medium. LDH activity is determined 

by a coupled enzymatic reaction. 

It oxidizes lactate to pyruvate, triggering 

the conversion of the yellow tetrazolium 

salt WST into the water-soluble red formazan 

dye. 

The increase in the amount of formazan 

produced in culture medium can be 

analyzed spectrophotometrically and 

correlates directly to the increase in the 

number of lysed cells. 

The kit can be used for many different in 

vitro cell systems to quantify cytotoxicity 

and cytolysis. 

AK Cytotoxicity Kit 

Lactate 

Formazan 

(red) 

LDH 

NAD + NADH + H + 

Catalyst 

The bioluminometric AK Cytotoxicity Kit 

is based on the measurement of adenylate 

kinase (AK), a ubiquitous protein 

present in all cells that is rapidly released 

into the culture medium upon damage 

to the plasma membrane. The simple 

one-step procedure involves two chemical 

reactions. The adenylate kinase release 

from the damaged cells catalyses 

the conversion of ADP to ATP. The 

luciferase catalyses a reaction between 

the ATP, oxygen, and luciferin producing 

light. The light can be measured using a 

luminometer or beta counter. This highly 

sensitive assay allows rapid quantitation 

of plasma membrane damage for evaluation 

of cell death or cytotoxicity events, 

and it is well suited for automation and 

high throughput. 

Fig. 5: Assay mechanism of the PromoKine LDH Cytotoxicity Kit II. 

Pyruvate 

WST-1 

(yellow) 


LDH Cytotoxicity Kit II 500 assays PK-CA577-K313 

AK Cytotoxicity Kit 500 assays PK-CA577-K312 


1.4 Senescence Detection Kit 

Applications 

Histochemical detection of senescent 

cells 

Cell senescence and oncology studies 

Features 

High sensitivity and specificity 

Compatible with common lab 

equipment 

Senescence is an arrested state in which 

the cells remain viable but are not stimulated 

to divide by serum or passage in 

culture. It is thought to be a tumor-suppressive 

mechanism and an underlying 

cause of aging. Senescent cells display an 

increase in size, senescence-associated 

expression of b-galactosidase (SA-b-Gal) 

activity, and altered patterns of gene 

expression. The PromoKine Senescence 

Detection Kit has been designed to histochemically 

detect a specific senescence 

marker (SA-b-Gal activity) in cultured 

cells and tissue sections. 

The specific histochemical marker is 

only present in senescent cells and is not 

found in presenescent, quiescent, or immortal 

cells. 


Senescence Detection Kit 250 assays PK-CA577-K320 

For prices please see our online shop at www.promokine.info


1.5 Cell-Based Reporter Assays 

Applications 

under these conditions, light emitted 

paring its fluorescence with that of a 

from the reaction is directly proportional 

GFP standard. The kit can detect a wide 

Monitoring and quantification of 

to the number of luciferase enzyme 

range of GFP concentrations (0.01-10 

reporter gene expression (Luciferase, 

molecules. The light emission from the 

μg/ml). Furthermore, a GFP quench 

b-Galactosidase, GFP) 

Determination of transfection 

efficiencies 

Features 

luciferase reaction can be recorded using 

a luminometer. 

The Luciferase Reporter HTS Kits have 

been specifically designed for economic, 

high-throughput applications using high- 

solution is provided for determining 

autofluorescence levels of cell or tissue 

extracts. 

Beta-Galactosidase Kits 


density microplates. 

Highly sensitive and reproducible 

In addition to the Firefly luciferase sub- 

The b-Galactosidase Staining Kit and the 

Fast and convenient protocols 

strate D-Luciferin, we also provide a 

b-Galactosidase Reporter Assay Kit have 

Compatible with common 

wide range of coelenterazine substrates 

been designed to detect and quantify 

plate readers 

for the Renilla luciferase catalysed reac- 

b-Gal activity in cells transfected with 

Luciferase Reporter Assay Kits 

tion. 

GFP Reporter Assay Kit 

a plasmid carrying the frequently-used 

LacZ reporter gene. This gene encodes 

b-galactosidase, an enzyme that is very 

The PromoKine ready-to-use Luciferase 

stable and resistant to proteolytic deg- 

Reporter Assays provide complete sys- 

GFP (Green Fluorescent Protein) is a flu- 

radation. When expressed in transfected 

tems for rapid, convenient quantita- 

orescent protein originally isolated from 

cells, it converts a colorless substrate 

tive detection of luciferase activity in 

the jellyfish Aequorea victoria. GFP and 

(e.g., CPRG, X-Gal, or ONPG) into a col- 

eukaryotic cells, e.g., cells transfected 

its enhanced variant EGFP have been 

ored reaction product that can be easily 

with plasmids carrying the Firefly and/ 

widely used as reporter proteins for in 

detected microscopically (b-Galactosi- 

or Renilla luciferase reporter genes. They 

vivo monitoring of gene expression in a 

dase Staining Kit) or quantified using a 

are optimized to provide excellent sensi- 

variety of cell types and organisms. Since 

spectrophotometer (b-Galactosidase Re- 

tivity as well as consistent light output 

GFP requires no additional substrates or 

porter Assay Kit). In addition, PromoKine 

when working with multiple samples. 

cofactors, GFP fluorescence can easily 

provides a range of chromogenic beta- 

Firefly luciferase catalyzes the oxidative 

be detected using a fluorescence micro- 

Galactosidase substrates. 

carboxylation of luciferin, a reaction 

scope. However, most imaging stud- 

with the highest efficiency of any known 

ies of GFP are qualitative. Quantitative 

bioluminescence reaction. Consequently, 

analyses of GFP expression levels in cells 

this sensitive reporter is ideally suited for 

or tissues are more informative and have 

studying gene regulation and function. 

wider applications. 

Renilla luciferase is often used in con- 

The GFP Reporter Assay Kit has been de- 

junction with Firefly luciferase as a 

signed for use in a 96-well micro-plate 

normalizing transfection control or for 

format (Ex/Em = 488 nm/507nm). Cells 

multiplexing transcriptional reporter as- 

or tissues can be homogenized directly 

says. Both Firefly luciferase and Renilla 

in the provided GFP assay buffer and the 

Fig. 6: b-Gal staining of CHO-K1 cells 

luciferase show maximum light output 

supernatant of the lysed cells/tissues is 

transfected with the pPK-CMV-R1 re- 

when substrates and co-factors are pres- 

transfered to the microwell plates. The 

porter vector expressing the lacZ gene 

ent in large quantities. When assayed 

amount of GFP is determined by com- 

(see chapter 4). 


Luciferase Reporter Assay Kit I (Firefly) 

Luciferase Reporter Assay Kit II (Renilla) 

Luciferase Reporter Assay Kit III 

(Firefly & Renilla) 

Luciferase Reporter HTS Kit (Firefly) 


150 assays 

1000 assays 

150 assays 

1000 assays 

100 assays 

1000 assays 

4 ml 

10 ml 

100 ml 

10 x 100 ml 

PK-CA707-30003-1 

PK-CA707-30003-2 

PK-CA707-30004-1 

PK-CA707-30004-2 

PK-CA707-30005-1 

PK-CA707-30005-2 

PK-CA707-30028-0 

PK-CA707-30028-1 

PK-CA707-30028-2 

PK-CA707-30028-3

12 




beta-Galactosidase Reporter Assay Kit 500 assays PK-CA708-10100 

beta-Galactosidase Staining Kit 50 assays PK-CA708-10300 

GFP Reporter Assay Kit 100 assays PK-CA577-K815 

Firefly Luciferase Substrates 

D-Luciferin, Free Acid 

D-Luciferin, Potassium Salt 

D-Luciferin, Sodium Salt 

10 mg 

50 mg 

1 g 

10 mg 

50 mg 

1 g 

10 mg 

50 mg 

1 g 

PK-CA707-10100 

PK-CA707-10100-1 

PK-CA707-10100-2 

PK-CA707-10101 

PK-CA707-10101-1 

PK-CA707-10101-2 

PK-CA707-10102 

PK-CA707-10102-1 

PK-CA707-10102-2 

DMNPE-caged D-Luciferin 5 mg PK-CA707-10103 

Renilla Luciferase Substrates 

Coelenterazine (native) 50 μg PK-CA707-10110 

Coelenterazine h 50 μg PK-CA707-10111 

Coelenterazine cp 50 μg PK-CA707-10112 

Coelenterazine hcp 50 μg PK-CA707-10113 

Coelenterazine f 50 μg PK-CA707-10114 

Coelenterazine n 50 μg PK-CA707-10115 

Coelenterazine ip 50 μg PK-CA707-10116 

Coelenterazine fcp 50 μg PK-CA707-10117 

Coelenterazine i 50 μg PK-CA707-10121 

Coelenterazine, 2-methyl 50 μg PK-CA707-10122 

Coelenterazine 400a 

50 μg PK-CA707-10125 

(also known as DeepBlue C, 

a trademark of Perkin Elmer) 

Coelenterazine Sampler Kit 1 Kit PK-CA707-10123 

Chromogenic beta-Galactosidase Substrates 

X-Gal 

100 mg 

1 g 

5 g 

PK-CA707-10011 

PK-CA707-10011-1 

PK-CA707-10011-2 

Red-beta-D-Gal 100 mg PK-CA707-10012 

Rose-beta-D-Gal 100 mg PK-CA707-10013 

Purple-beta-D-Gal 25 mg PK-CA707-10014 

Green-beta-D-Gal 25 mg PK-CA707-10015 

Chromogenic beta-Galactosidase 

Substrate Sampler Kit 


1 kit (5 x 5 mg) PK-CA707-10024 

Please see chapter 4 for luciferase, beta-Gal, and GFP reporter vectors.


1.6 Cell Staining Reagents and Related Products 

Applications 

Detection of cellular structures 

Fluorescence microscopy/Flow 

cytometry 

Features 

High quality 

Thoroughly tested 


Fluorescent dyes offer extraordinary sensitivity 

and extended possibilities for the 

visualization and analysis of intracellular 

processes and cell structures such as the 

cell membrane, mitochondria, the nucleus, 

and the cytoskeleton. Some dyes 

are also suited for staining only viable 

or dead cells. That is an important feature 

for elucidating the effects of certain 

agents on cell viability. 

PromoKine provides a select choice of 

reagents for staining different cell organelles 

and structures as well as for 

determining processes like membrane 

potential changes. Some of these reagents 

are available as esters, which pass 

through the membranes of viable cells. 

Once inside, the ester group is hydrolyzed 

by cellular esterases, and the dye 

can accumulate in the cell. Some dyes 

are nonfluorescent in their ester form but 

fluoresce strongly when the ester group 

has been removed. 

Fig. 7: Primary human fibroblasts (NHDF, 

PromoCell) stained with Calcein and 

counterstained with DAPI. 


Product Product Description Size Catalog Number 

Nucleus Stains 

7-AAD 

(7-aminoactinomycin D) 

Fluorescent DNA intercalator 1 mg PK-CA707-40037 

Acridine Orange (AO) 

Solution 

Nucleus stain (DNA/RNA) 

10 ml 

(10 mg/ml) 

PK-CA707-40039 

DAPI Nucleus stain (DNA) 10 mg PK-CA707-40011 

DAPI Solution Nucleus stain (DNA); aqueous solution 10 mg in 1 ml PK-CA707-40043 

DAPI, dilactate 4’,6-diamidino-2-phenylindole, dilactate 10 mg PK-CA707-40009 

EB (ethidium bromide) 

Solution 

Dead cell stain (DNA), aqueous solution 

10 ml 

(10 mg/ml) 

PK-CA707-40042 

Ethidium Homodimer I Nucleus stain (DNA); also known as EthD-1, or EtDi 1 mg PK-CA707-40010 

Ethidium Homodimer III Nucleus stain (DNA) 1 mg PK-CA707-40050 

Ethidium Homodimer III, Nucleus stain (DNA); in DMSO 200 µl PK-CA707-40051 

1 mM in DMSO 

Hoechst 33258 Nucleus stain (DNA) 100 mg PK-CA707-40045 

Hoechst 33258 Solution Nucleus stain (DNA); aqueous solution 10 ml PK-CA707-40044 

(10 mg/ml) 



(10 mg/ml) 

Propidium Iodide (PI) Dead cell stain (DNA) 100 mg PK-CA707-40016 

Propidium Iodide (PI) Solution Dead cell stain (DNA); aqueous solution 10 ml PK-CA707-40017 

(1 mg/ml) 

Mitochondria Stains 

DASPEI 

2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide; 

100 mg PK-CA707-70018 

for staining of mitochondria in live cells 

Dihydrorhodamine 123 Reduced form of Rhodamine 123; mitochondria stain 10 mg PK-CA707-10055 

Dihydrorhodamine 123, 

dihydrochloride salt 

DiOC 6 

(3) 

JC-1 chloride 

Reduced form of Rhodamine 123; mitochondria stain 10 mg PK-CA707-10056 

3,3’-dihexyloxacarbocyanine, iodide; membrane 

potential sensitive dye 

Stains mitochondria in living cells in a membrane 

potential-dependent function 


100 mg PK-CA707-70009 

5 mg PK-CA707-70011

14 




JC-1 iodide 

Nonyl Acridine Orange 

(or NAO) 

Stains mitochondria in living cells in a membrane 

potential-dependent function 

Mitochondria stain; not dependent on mitochondrial 

membrane potential 

5 mg PK-CA707-70014 

50 mg PK-CA707-70012 

Rhodamine 123 Green-fluorescent mitochondria stain 50 mg PK-CA707-70010 

Tetrabromorhodamine 123, 

bromide 

TMRE 

TMRM 

ER Stains 

DiOC 6 

(3) 

Green-fluorescent mitochondria stain 5 mg PK-CA707-70013 

Tetramethylrhodamine ethyl ester, perchlorate; 

membrane potential sensitive dye 

Tetramethylrhodamine methyl ester, perchlorate; 


3,3’-dihexyloxacarbocyanine, iodide; 


25 mg PK-CA707-70016 

25 mg PK-CA707-70017 

100 mg PK-CA707-70009 

Viable Cell Stains 

BCECF Viable cell stain (cytosol) 1 mg PK-CA707-51010 

BCECF AM Viable cell stain (cytosol); acetoxymethyl ester 10 x 100 µg 

20 x 50 µg 

1 mg 

BCECF AM Solution 

Viable cell stain (cytosol); acetoxymethyl ester; 

DMSO solution 

Calcein AM Viable cell stain (cytosol); acetoxymethyl ester 20 x 50 µg 

1 mg 

Calcein AM Solution, 

1 mg/ml in anhydrous DMSO 


4 mM DMSO solution 

Viable cell stain (cytosol); acetoxymethyl ester, 

DMSO solution 

Viable cell stain (cytosol); acetoxymethyl ester, 


PK-CA707-51011 

PK-CA707-51011-1 

PK-CA707-51012 

1 mg in 1 ml PK-CA707-51009 

PK-CA707-80011-3 

PK-CA707-80011 

1 mg in 1 ml PK-CA707-80011-2 

100 µl PK-CA707-80011-1 

Calcein, high purity Viable cell stain (cytosol); ultra-pure 100 mg PK-CA707-80013 

CFSE; 5(6)-CFDA, SE 

5-(and-6)-carboxyfluorescein diacetate, 

25 mg PK-CA707-90041 

succinimidyl ester; viable cell stain (cytosol) 



(10 mg/ml) 


Hoechst 33342 Solution Nucleus stain (DNA); aqueous solution 10 ml 

(10 mg/ml) 

Dead Cell Stains 

EB (ethidium bromide) 

Solution 

Dead cell stain (DNA); aqueous solution 

10 ml 

(10 mg/ml) 

PK-CA707-40046 

PK-CA707-40042 

Propidium Iodide (PI) Dead cell stain (DNA) 100 mg PK-CA707-40016 

Propidium Iodide (PI) Solution Dead cell stain (DNA); aqueous solution 10 ml (1 mg/ml) PK-CA707-40017 

Trypan Blue Solution (0.4%) Dead cell stain; tetrasodium salt, 0.4% saline solution 10 ml PK-CA577-1209 

Cytoplasmic Membrane Staining 

Blue-fluorescent Cytoplasmic 

Membrane Staining Kit 

Green-fluorescent Cytoplasmic 


Orange-fluorescent Cytoplasmic 


Red-fluorescent Cytoplasmic 


Convenient and sensitive cell staining 

with low cytotoxicity. 







50 assays PK-CA707-30024 

1 ml PK-CA707-30021 

1 ml PK-CA707-30022 

1 ml PK-CA707-30023 




Lipophilic Carbocyanine Dyes 

DiD (DiIC 18 

[5]) 

DiI (or DiIC1 8 

[[3]) 

1,1'-dioctadecyl-3,3,3',3'- tetramethylindodicarbocyanine, 

4-chlorobenzenesulfonate salt 

1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine 

perchlorate 

50 mg PK-CA707-60014 

50 mg PK-CA707-60010 

DiI in vegetable oil Otimized DiI formulation for microinjection 0.5 ml PK-CA707-60018 

Dilinoleyl DiI 

1,1'-dilinoleyl-3,3,3',3'-tetramethylindocarbocyanine 

5 mg PK-CA707-60034 

perchlorate (also called Fast DiI, a trademark of 

Molecular Probes) 

Dilinoleyl DiO 

1,1'-dilinoleyl-3,3'-oxacarbocyanine perchlorate 

5 mg PK-CA707-60035 

(also called Fast DiO, a trademark of Molecular Probes) 

DiO (or DiOC 18 

[3]) 3,3'-dioctadecyloxacarbocyanine, perchlorate 50 mg PK-CA707-60011 

DiOC 14 

(3), 

hydroxyethanesulfonate 

DiOC 16 

(3) 

DiR (DiIC 18 

[7]) 

3,3'-ditetradecyloxacarbocyanine, 

hydroxyethanesulfonate 

Lipophilic cyanine dye for staining cytoplasmic 

membranes 

1,1'-dioctadecyl-3,3,3',3'-tetramethyl 

indotricarbocyanine Iodide 

50 mg PK-CA707-60012 

25 mg PK-CA707-60038 

25 mg PK-CA707-60017 

Neuro-DiI Improved alternative to DiI 25 mg PK-CA707-60016 

Neuro-DiI in vegetable oil Otimized formulation for microinjection 0.2 ml PK-CA707-60020 

Neuro-DiO Improved alternative to DiO 25 mg PK-CA707-60015 

Neuro-DiO in vegetable oil Otimized formulation for microinjection 0.2 ml PK-CA707-60019 

Phospholipid Membrane Probes 

Biotin-DHPE 

N-(biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3- 

10 mg PK-CA707-60022 

phosphoethanolamine, triethylammonium salt 

Biotin-X-DHPE 

N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, 

5 mg PK-CA707-60023 

triethylammonium 

salt 

Fluorescein-DHPE 

N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, 

5 mg PK-CA707-60024 

triethylammoniumsalt 

NBD-DHPE 

N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, 

10 mg PK-CA707-60025 

triethylam- 

monium salt 

Rhodamine-DHPE 

N-(lissamine rhodamine B sulfonyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, 

5 mg PK-CA707-60026 

triethylammonium 

salt 

Texas Red-DHPE 

N-(Texas Red sulfonyl)-1,2-dihexadecanoyl-sn-glycero- 

1 mg PK-CA707-60027 

3-phosphoethanolamine, triethylammonium salt 

TRITC-DHPE 

N-(teramethylrhodamine-6-thiocarbamoyl)-1,2- 

1 mg PK-CA707-60028 

dihexadecanoyl-sn-glycero-3-phosphoethanolamine, 

triethylammonium salt 

Other Membrane Dyes 

AM3-25 

Lipophilic styryl dye with an amine group at the 

1 mg PK-CA707-70051 

hydrophilic head 

Biotin-AM3-25 

AM3-25 conjugate with biotin that can be coupled 

1 mg PK-CA707-60032 

to avidin or streptavidin 

DiA 4-(4-dihexadecylaminostyryl)-N-methylpyridinium iodide 50 mg PK-CA707-60013 

DiB 

DPA 

NBD C6-ceramide 

Lipophilic blue fluorescent dye useful for staining 

cytoplasmic membranes. 

Nonfluorescent anionic membrane dye that can be used 

as a FRET quencher for a variety of fluorescent donor 

dyes. 

6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino) 

hexanoyl)sphingosine 

10 mg PK-CA707-60036 

25 mg PK-CA707-60037 

1 mg PK-CA707-60030 



16 




NBD C6-sphingomyelin 

Octadecyl Rhodamine B 

Chloride (R18) 

Nerve Terminal Staining Kits 

Nerve Terminal Green 

Staining Kit I 


Staining Kit II 


Staining Kit III 


Staining Kit IV 

Nerve Terminal Red 

Staining Kit I 

Nerve Terminal Red 

Staining Kit II 

6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino) 

1 mg PK-CA707-60031 

hexanoyl)sphingosyl phosphocholine 

Useful for membrane fusion assays 10 mg PK-CA707-60033 

Kit contains 5 x 1 mg SynapseGreen C4 

1 set PK-CA707-70030 

and 250 mg ADVASEP-7 

Kit contains 1 mg AM1-43 and 100 mg ADVASEP-7 1 set PK-CA707-70031 

Kit contains 1 mg AM1-43 and 100 mg SCAS 1 set PK-CA707-70031-1 

Kit contains 5 x 1 mg SynapseGreen C4 

and 100 mg Sulforhodamine 101 

Kit contains 5 x 1 mg SynapseRed C2 

and 250 mg ADVASEP-7 

Kit contains 5 x 1 mg SynapseRed C2 

and 100 mg Sulforhodamine 101 

1 set PK-CA707-70032 

1 set PK-CA707-70034 

1 set PK-CA707-70035 

Nerve Terminal Probes & Related Reagents 

ADVASEP-7 

For background fluorescence reduction 250 mg PK-CA707-70029 

(sulfobutyl b-cyclodextrin) 

Alpha-Bungarotoxin 

(from Bungarus multicinctus) 

Acetylcholine receptor inhibitor 

1 mg 

5 mg 

PK-CA707-00010-1 

PK-CA707-00010 

AM1-43 

Fixable, activity-dependent fluorescent nerve 

1 mg PK-CA707-70024 

terminal probe 

AM1-44 Nerve Terminal Probe 1 mg PK-CA707-70038 





Biotin-XX-a-Bungarotoxin 

Fluorescein-a-Bungarotoxin 

SCAS 

Sulforhodamine 101-a- 

Bungarotoxin 

Can be used to isolate and/or image the distribution of 

the nicotinic acetylcholine receptor at the neuromuscular 

junctions 

Can be used to image the distribution of the nicotinic 

acetylcholine receptor at the neuromuscular junctions 

4-sulfonato calix[8]arene, sodium salt; for background 

fluorescence reduction 

500 µg PK-CA707-00017 

500 µg 

10 x 50 µg 

Also known as Texas Red-a-Bungarotoxin 500 µg 

10 x 50 µg 

SynapseGreen C1 Nerve Terminal Probe 5 mg 

5 x 1 mg 

SynapseGreen C18 

(FM3-25) 

SynapseGreen C2 (FM2-10) 

Nerve Terminal Probe (also known as FM3-25, 

a trademark of Molecular Probes, Inc.) 



PK-CA707-00011 

PK-CA707-00013 

100 mg PK-CA707-70037 

5 mg 

5 x 1 mg 

5 mg 

5 x 1 mg 

SynapseGreen C3 Nerve Terminal Probe 5 mg 

5 x 1 mg 







5 mg 

5 x 1 mg 

5 mg 

5 x 1 mg 

SynapseRed C1 Nerve Terminal Probe 5 mg 

5 x 1 mg 

SynapseRed C2 (FM4-64) 

Nerve Terminal Probe (also known as FM4-64, a trademark 

of Molecular Probes, Inc.) 


5 mg 

5 x 1 mg 

PK-CA707-00015 

PK-CA707-00016 

PK-CA707-70042 

PK-CA707-70043 

PK-CA707-70048 

PK-CA707-70049 

PK-CA707-70044 

PK-CA707-70045 

PK-CA707-70023 

PK-CA707-70026 

PK-CA707-70020 

PK-CA707-70022 

PK-CA707-70046 

PK-CA707-70047 

PK-CA707-70040 

PK-CA707-70041 

PK-CA707-70021 

PK-CA707-70027



SynapseRed C2M (FM5-95) 

Tetramethylrhodaminea-Bungarotoxin 

Membrane Potential Sensitive Dyes 

DiO/DPA Membrane 

Potential Detection Kit 

Nerve Terminal Probe (also known as FM5-95, a trademark 

of Molecular Probes, Inc.) 

Can be used to image the distribution of the nicotinic 

acetylcholine receptor at the neuromuscular junctions 

For fluorescent detection of cytoplasmic 

membrane potential changes 

5 mg 

5 x 1 mg 

500 µg 

10 x 50 µg 

PK-CA707-70028 

PK-CA707-70019 

PK-CA707-00012 

PK-CA707-00014 

1 kit PK-CA707-30037 

Di-12-ANEPPQ Fast-reponding membrane potential dye 5 mg PK-CA707-61015 

Di-2-ANEPEQ (JPW1114) Fast-reponding membrane potential dye; 

5 mg PK-CA707-61013 

also called JPW1114 

Di-4-ANEPPS Fast-reponding membrane potential dye 5 mg PK-CA707-61010 

Di-8-ANEPPQ Fast-reponding membrane potential dye 5 mg PK-CA707-61014 

Di-8-ANEPPS Fast-reponding membrane potential dye 5 mg PK-CA707-61012 

DiBAC 4 

(3) 

Bis-(1,3-dibarbituric acid)-trimethine oxanol; 

25 mg PK-CA707-61011 


DiIC 1 

(5) 

1,1'-dimethyl-3,3,3',3'-tetramethylindodicarbocyanine, 

100 mg PK-CA707-70015 

iodide 

DiOC 2 

(3) 

3,3’-diethyloxacarbocyanine, iodide; 

100 mg PK-CA707-70008 


DiOC 5 

(3) 

3,3’-dipentyloxacarbocyanine, iodide; 

100 mg PK-CA707-70007 


DiOC 6 

(3) 

3,3’-dihexyloxacarbocyanine, iodid; 

100 mg PK-CA707-70009 


RH237 Fast-responding potentiometric probe 5 mg PK-CA707-61018 


RH421 Fast-reponding membrane potential dye 25 mg PK-CA707-61017 


TMRE 

Tetramethylrhodamine ethyl ester, perchlorate; 

25 mg PK-CA707-70016 


TMRM 

Tetramethylrhodamine methyl ester, perchlorate; 

25 mg PK-CA707-70017 


Cytosolic Tracers and Related Reagents 

Biocytin (e-biotinoyl–L-lysine) Can be introduced into cells by microinjection 100 mg PK-CA707-90055 

Biocytin-Hydrazide 

Can be introduced into cells by microinjection; 

25 mg PK-CA707-90060 

aldehyde-fixable 

Biotin-4-Fluorescein Can be used as polar tracer to study cell morphology 10 mg PK-CA707-90062 

Biotin-Ethylenediamine, 

hydrobromide 

Biotin-Ethylenediamine, 

hydrochloride 

Equivalent to Neurobiotin; transneuronal tracer 25 mg PK-CA707-90057 

Functionally equivalent to Neurobiotin; 

transneuronal tracer 

25 mg PK-CA707-90075 

Biotin-Rhodamine 110 Can be used as polar tracer to study cell morphology 5 mg PK-CA707-80022 

Calcein AM Viable cell stain (cytosol); acetoxymethyl ester 1 mg 

20 x 50 µg 


1 mg/ml in anhydrous DMSO 



PK-CA707-80011 

PK-CA707-80011-3 

Viable cell stain (cytosol); acetoxymethyl ester 1 ml PK-CA707-80011-2 

Viable cell stain (cytosol); acetoxymethyl ester 100 µl PK-CA707-80011-1 

Calcein, high purity Viable cell stain (cytosol); ultra-pure 100 mg PK-CA707-80013 

DPA/Terbium for 

Can be used for vesicle fusion assays 1 set PK-CA707-80104 

membrane fusion assay 

DPX Can be used to study membrane fusion or permeability 500 mg PK-CA707-80012 



18 




Fluorescein-Biotin 

5-((N-(5-(N-(6-(biotinoyl)amino)hexanoyl)amino)pentyl) 

5 mg PK-CA707-80019 

thioureidyl) fluorescein 

Hydroxystilbamidine Also known as FluoroGold TM 10 mg PK-CA707-80014 

Hydroxystilbamidine 4% in H 2 

O; also known as FluoroGold TM 200 µl PK-CA707-80023 

Lucifer Yellow Cadaverine Can be used as fixable fluorescent tracer 25 mg PK-CA707-80018 

Lucifer Yellow Cadaverine Useful tracer for studying neuronal morphology 10 mg PK-CA707-80017 

Biotin-X, dipotassium salt 

Lucifer Yellow CH lithium salt Useful polar tracer for studying neuronal morphology 25 mg PK-CA707-80015 

Lucifer Yellow CH potassium 

salt 

RuBP-2S 

RuBP-4S 

RuBPS 

SDIP/Europium 

for membrane fusion assay 

Sulforhodamine 101 

Sulforhodamine B 

Sulforhodamine G 

Useful polar tracer for studying neuronal morphology 25 mg PK-CA707-80016 

Ruthenium(bis(bathophenanthroline))(bathophenanthrolinedisulfonate 

5 mg PK-CA707-80024 

Ruthenium(bathophenanthroline)(bis(bathophenanthroli 

5 mg PK-CA707-80025 

nedisulfonate)), disodium salt 

Ruthenium(tris(bathophenanthrolinedisulfonate), 

5 mg PK-CA707-80021 

tetrasodium salt 

Can be used for vesicle fusion assays 1 set PK-CA707-80105 

Useful polar tracer for studying neuronal morphology 

and cell-cell communication 





500 mg PK-CA707-80101 

5 g PK-CA707-80100 

5 g PK-CA707-80102 

Related Products 

DMSO, anhydrous Dimethylsulfoxide, anhydrous 10 ml PK-CA707-90082 

Pluronic F-127 

Nonionic detergent useful for solubilizing large dye 

1 g PK-CA707-59000 

molecules such as Fura-2/AM, Fluo-3/AM, Indo-1/AM 

and Rhod-2/AM to facilitate cell loading. 

Pluronic F-127, 

20% solution in DMSO 




1 ml PK-CA707-59004 

Pluronic F-127, 

10% in H 2 

O 





30 ml PK-CA707-59005


1.7 Intracellular Indicators and Related Products 

Signal transduction often starts with the 

Derivatives for special applications 

Fluo-3FF, and Indo-1FF which are par- 

activation of a receptor on the cell sur- 

available 

ticularly useful for certain applications 

face which subsequently triggers a cas- 

Compatible with standard fluores- 

(e.g., spatial and functional characteri- 

cade of second messengers in the cell. 

cence equipment 

zation of Ca 2+ stores or detection of 

The change of intracellular ion concentration 

is a crucial step that helps second 

messengers transmit information. Consequently, 

monitoring and control of in- 

Calcium ions are important second messengers 

in cells. They are involved in 

muscle contraction, nerve signal trans- 

NMDA-induced neuronal Ca 2+ fluxes). 

PromoKine also provides a range of nonfluorescent 

calcium chelators as well as 

coelenterazine derivatives which are ef- 


tracellular ion concentration is a prereq- 

mission, immune cell activation, hor- 

fective luminescent calcium indicators. 

uisite understanding second messengers 

mone secretion, etc. 

and signal transduction in living cells. 

Fluorescent calcium indicators selectively 

bind to calcium ions and show a spec- 

Other Indicators 

Calcium Indicators 

tral response upon binding Ca 2+ , i.e., 

fluorescence wavelength or intensity are 

Applications 

altered. This shift of fluorescence wave- 

Applications 

length and change of intensity upon cal- 

pH determination 

cium chelation can be monitored using 

Iron, zinc, and chloride ion detection 

Intracellular calcium signaling studies 

Investigation of signal transduction 

fluorescence microscopy. 

PromoKine offers a wide range of fluo- 

Features 

and apoptotic cascades 

rescent calcium indicators for detecting 

Neuroscience research 

changes in the intracellular Ca 2+ concen- 

High quality and sensitivity 

tration, including Fura-2, Fluo-3, Rhod- 

Features 

2, and Indo-1 (as cell-impermeable salts 

In addition to our calcium probes, 

or cell-permeable AM esters) as well as 

PromoKine also offers pH indicators as 

High quality and sensitivity 

their low-affinity derivatives Fura-2FF, 

well as indicators for iron, zinc, and chloride 

ion detection. 

n Calcium Indicators 


Fluorescent Calcium Indicators 

Fluo-3 (pentaammonium salt) Fluorescent calcium indicator; Ex/Em: 490-506/526 1 mg PK-CA707-50010 

Fluo-3 (pentapotassium salt) Fluorescent calcium indicator; Ex/Em: 490-506/526 1 mg PK-CA707-50011 

Fluo-3 (pentasodium salt) Fluorescent calcium indicator; Ex/Em: 490-506/526 1 mg PK-CA707-50012 

Fluo-3 AM Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg 

20 x 50 µg 

1 mg 

Fluo-3 AM Solution 

Fluorescent calcium indicator (acetoxymethyl ester; 1 mM 

in anhydrous DMSO) 

PK-CA707-50013 

PK-CA707-50016 

PK-CA707-50014 

1 ml PK-CA707-50015 

Fura-2 (pentaammonium salt) Fluorescent calcium indicator; Ex/Em: 340-380/510 1 mg PK-CA707-50030 

Fura-2 (pentapotassium salt) Fluorescent calcium indicator; Ex/Em: 340-380/510 1 mg PK-CA707-50031 

Fura-2 (pentasodium salt) Fluorescent calcium indicator; Ex/Em: 340-380/510 1 mg PK-CA707-50032 

Fura-2 AM Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg 

20 x 50 µg 

1 mg 

Fura-2 AM Solution 

Fluorescent calcium indicator (acetoxymethyl ester; 1 mM 

in anhydrous DMSO) 

PK-CA707-50033 

PK-CA707-50033-1 

PK-CA707-50034 

1.0 ml PK-CA707-50029 

Indo-1 (pentaammonium salt) Fluorescent calcium indicator; Ex/Em: 330/405-485 1 mg PK-CA707-50040 

Indo-1 (pentapotassium salt) Fluorescent calcium indicator; Ex/Em: 330/405-485 1 mg PK-CA707-50041 

Indo-1 (pentasodium salt) Fluorescent calcium indicator; Ex/Em: 330/405-485 1 mg PK-CA707-50042 

Indo-1 AM Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg 

20 x 50 µg 

1 mg 

PK-CA707-50043 

PK-CA707-50043-1 

PK-CA707-50044 

Rhod-2 (triammonium salt) Fluorescent calcium indicator; Ex/Em: 533-556/~578 1 mg PK-CA707-50020 


20 




Rhod-2 (tripotassium salt) Fluorescent calcium indicator; Ex/Em: 533-556/~578 1 mg PK-CA707-50021 

Rhod-2 (trisodium salt) Fluorescent calcium indicator; Ex/Em: 533-556/~578 1 mg PK-CA707-50022 

Rhod-2 AM Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg 

1 mg 

Fluorescent High Calcium Indicators 

5,5'-dibromo-Bapta 

(tetrapotassium salt) 

5,5'-difluoro-Bapta 


PK-CA707-50023 

PK-CA707-50024 

Fluorescent calcium indicator 100 mg PK-CA707-50004 


5,5'-difluoro-Bapta AM Fluorescent calcium indicator (acetoxymethyl ester) 25 mg PK-CA707-50005 

Bis-Fura-2, hexapotassium salt Fluorescent calcium indicator 1 mg PK-CA707-50045 

Fura-2FF (K + Salt) Difluorinated derivative of Fura-2 (K + Salt) 500 μg PK-CA578-F1046 

Fura-2FF AM Difluorinated derivative of Fura-3 (acetoxymethyl ester) 500 μg PK-CA578-F1044 

Furaptra, AM ester Fluorescent calcium indicator (also known as Mag-Fura-2) 10 x 100 µg 

20 x 50 µg 

1 mg 

PK-CA707-50037 

PK-CA707-50039 

PK-CA707-50038 

Furaptra, tetrapotassium salt Fluorescent calcium indicator (also known as Mag-Fura-2) 1 mg PK-CA707-50035 

Furaptra, tetrasodium salt Fluorescent calcium indicator (also known as Mag-Fura-2) 1 mg PK-CA707-50036 

Indo-1FF (K + Salt) Difluorinated derivative of Indo-1 (K + Salt) 500 μg PK-CA578-I1086 

Indo-1FF AM Difluorinated derivative of Indo-1 (acetoxymethyl ester) 500 μg PK-CA578-I1084 

Fluorescent Near-Membrane Calcium Indicators 

FFP-18 AM 

Fluorescent near-membrane calcium indicator 

500 μg PK-CA578-F1052 

(Fura-derivative; acetoxymethyl ester) 

FIP-18 AM 

Fluorescent near-membrane calcium indicator 

500 ug PK-CA578-F1054 

(Indo-derivative; acetoxymethyl ester)) 

Calcium Chelators 

5,5'-dibromo-Bapta 



5,5'-dimethyl BAPTA, AM Fluorescent calcium indicator (acetoxymethyl ester) 25 mg PK-CA707-50007 

ester 

5,5'-dimethyl BAPTA, Fluorescent calcium indicator 100 mg PK-CA707-50008 

tetrapotassium salt 

Bapta (tetracesium salt) Non-fluorescent calcium chelator 1 g PK-CA707-50001 

Bapta (tetrapotassium salt) Non-fluorescent calcium chelator 1 g PK-CA707-50002 

Bapta (tetrasodium salt) Non-fluorescent calcium chelator 1 g PK-CA707-50003 

Bapta AM Non-fluorescent calcium chelator (acetoxymethyl ester) 25 mg 

20 x 1 mg 

PK-CA707-50000 

PK-CA707-50000-1 

Bapta-FF (free acid) Difluorinated derivative of BAPTA 10 mg PK-CA578-B1091 

Bapta-FF AM Difluorinated derivative of BAPTA (acetoxymethyl ester) 10 mg PK-CA578-B1093 

Luminescent Calcium Indicators 

Coelenterazine (native) Coelenterazine; luminescent calcium indicator 

50 μg PK-CA707-10110 

& luciferase substrate 

Coelenterazine 400a 

Coelenterazine; luminescent calcium indicator 

50 μg PK-CA707-10125 

& luciferase substrate (also known as DeepBlue C, 

a trademark of Perkin Elmer) 

Coelenterazine cp 

Coelenterazine derivative; luminescent calcium indicator 

50 μg PK-CA707-10112 


Coelenterazine f 


50 μg PK-CA707-10114 


Coelenterazine fcp 



50 μg PK-CA707-10117 




Coelenterazine h 


50 μg PK-CA707-10111 


Coelenterazine hcp 


50 μg PK-CA707-10113 


Coelenterazine i 


50 μg PK-CA707-10121 


Coelenterazine ip 


50 μg PK-CA707-10116 


Coelenterazine n 


50 μg PK-CA707-10115 


Coelenterazine, 2-methyl Coelenterazine derivative; luminescent calcium indicator 

50 μg PK-CA707-10122 


Coelenterazine Sampler Kit Coelenterazine and derivatives thereof 1 Kit PK-CA707-10123 


n Other Indicators 


Zn Indicators 

Zinquin, ethyl ester Blue-fluorescent Zn indicator 5 mg PK-CA707-52020 

Zinquin, free acid Blue-fluorescent Zn indicator 5 mg PK-CA707-52022 

Cl Indicators 

MQAE 

N-(ethoxycarbonylmethyl)-6-methoxyquinolinium 

100 mg PK-CA707-52011 

bromide; fluorescent chloride ion indicator 

SPQ 

6-methoxy-N-(3-sulfopropyl)quinolinium; 

50 mg PK-CA707-52010 

fluorescent chloride ion indicator 

Heavy Metal Ion Indicators 

TPEN 

Tetrakis-(2-pyridylmethyl)ethylenediamine; 

100 mg PK-CA707-59003 

heavy metal chelator 

pH Indicators 

5(6)-CFDA 

5-(and-6)-carboxyfluorescein diacetate; 

100 mg PK-CA707-51014 

fluorescent pH indicator 

5(6)-CFDA, SE (CFSE) 5-(and-6)-carboxyfluorescein diacetate, 

25 mg PK-CA707-90041 

succinimidyl ester 

5-(and-6)-Carboxy-2',7'- Fluorescent pH indicator 200 mg PK-CA707-51015 

dichlorofluorescein 

5-(and-6)-Carboxy-2',7'- Fluorescent pH indicator; cell membrane-permeant 100 mg PK-CA707-51016 

dichlorofluorescein diacetate 

5-(and-6)-Carboxy-2',7'- Fluorescent pH indicator; cell membrane-permeant 25 mg PK-CA707-90040 

dichlorofluorescein diacetate, 

succinimidyl ester (CDFDA, 

SE) 

5-(and-6)-Carboxyfluorescein Fluorescent pH indicator 100 mg PK-CA707-51013 

5-Carboxy-2',7'- 

dichlorofluorescein 

5-Carboxy-2',7'- 

dichlorosulfonefluorescein 

5-CFDA 

Fluorescent pH indicator (single isomer) 100 mg PK-CA707-51017 

Fluorescent pH indicator (single isomer) 10 mg PK-CA707-51023 

5-Carboxyfluorescein diacetate (single isomer); 


100 mg PK-CA707-51018 

5-FAM 5-Carboxyfluorescein; fluorescent pH indicator 100 mg PK-CA707-51019 

6-CFDA 

6-Carboxyfluorescein diacetate (single isomer); 



100 mg PK-CA707-51021

22 




6-FAM 6-carboxyfluorescein; fluorescent pH indicator 100 mg PK-CA707-51020 

BCECF 

2',7'-bis-(carboxyethyl)-5-(and-6)-carboxyfluorescein; 


BCECF AM Fluorescent pH indicator; acetoxymethyl ester 10 x 100 µg 

20 x 50 µg 

1 mg 

BCECF AM Solution 

Fluorescent pH indicator; acetoxymethyl ester 

(1 mg/ml in dry DMSO) 

1 mg PK-CA707-51010 

PK-CA707-51011 

PK-CA707-51011-1 

PK-CA707-51012 

1 ml PK-CA707-51009 

Flubida-2 Fluorescent pH indicator 5 mg PK-CA707-51022 

n Related Products 


4-Bromo A-23187 free acid For in situ calibration of fluorescent calcium indicators 1 mg PK-CA707-59006 

A-23187 free acid A-23187 Free Acid (calcimycin; or Calcium Ionophore III) 1 mg 

10 mg 

PK-CA707-59001 

PK-CA577-1501 

Calcium Calibration Buffer Kit For calibration of fluorescent calcium indicators 2 x 50 ml PK-CA707-59100 

DMSO, anhydrous Dimethylsufoxide, anhydrous 10 ml PK-CA707-90082 

EDC (EDAC) 

1-(3-dimethylaminopropyl-3-ethylcarbodiimide, 

100 mg PK-CA707-59002 

hydrochloride) 

Flubi-2 Calibration standard for Flubida-2 5 mg PK-CA707-51024 

Ionomycin (calcium salt) 

Ionomycin (free acid) 

Pluronic F-127 

Pluronic F-127, 10% in H 2 

O 

Pluronic F-127, 20% solution 

in DMSO 

Useful in cell activation experiments when calcium 

dose-response data are not required (>90% pure) 

Useful for studies of Ca 2+ transport across biological 

membranes and measurement of cytoplasmic free Ca 2+ ; 

induces apoptotic neuronal degeneration in embryonic 

cortical neurons (>90% pure) 


molecules such as Fura-2/AM, Fluo-3/AM, 

Indo-1/AM and Rhod-2/AM to facilitate cell loading. 








5 mg PK-CA577-1566-5 

5 mg PK-CA577-1565-5 

1 g PK-CA707-59000 

30 ml PK-CA707-59005 

1 ml PK-CA707-59004


1.8 Cell Signaling Assays 

Applications 

Guanosine 3,5-cyclic monophosphate 

pmol cGMP can be detected. This is 

(cyclic GMP, cGMP) has been shown to 

approximately 10 times more sensitive 

Quantitative determination of 

be present at levels typically 10-100 fold 

than similar kits. 

cAMP/cGMP levels 

Cell signaling and cell growth/ 

differentiation studies 

b-Secretase activity detection 

Alzheimer’s disease drug development 

lower than cAMP in most tissues and is 

formed by the action of the enzyme guanylate 

cyclase on GTP. Some hormones 

(e.g., acetylcholine, insulin, and oxytocin) 

and chemicals (e.g., serotonin and 

b-Secretase Fluorometric Assay Kit 

b-Secretase (BACE) is a membrane-bound 

aspartyl protease that cleaves the amyloid 


Features 

histamine) cause an increase in cGMP 

levels. 

precursor protein and is consequently an 

excellent target for anti-amyloid therapy 

in the treatment of Alzheimer’s disease. 

Fast and simple assays 

The PromoKine cAMP and cGMP com- 

Finding inhibitors of b-secretase is one 


petitive immunoassays have been devel- 

of the major goals of Alzheimer’s dis- 

cAMP and cGMP Immunoassay Kits 

oped for the quantitative determination 

of cAMP and cGMP levels. They utilize 

ease drug development. The PromoKine 

b-Secretase Fluorometric Assay provides 

polyclonal capture antibodies against 

a convenient, non-radioactive system for 

Adenosine 3’,5’-cyclic monophosphate 

cAMP or cGMP coated on a 96-well 

detecting b-secretase activity in biologi- 

(cyclic AMP, cAMP) is one of the most 

plate and HRP-conjugated cAMP or 

cal samples. The kit provides active b- 

important second messengers involved 

cGMP-HRP which competes with the 

secretase as positive control, b-secretase 

as a modulator in physiological process- 

cAMP or cGMP from the sample. After 

inhibitor as negative control, optimized 

es. It regulates neuronal, glandular, and 

HRP-substrate addition, the amount of 

peptide substrate (conjugated to two 

cardiovascular processes as well as im- 

cAMP-HRP or cGMP-HRP bound to the 

reporter molecules), and buffers for 

mune mechanisms, the nervous system, 

plate can easily be determined spectro- 

convenient measurement of b-secretase 

cell growth, and cell differentiation. A 

photometrically at 450 nm. The intensity 

activity in mammalian samples. 

number of hormones (e.g. ACTH, TSH, 

of the blue color is inversely proportional 

b-Secretase inhibitors are also available 

LH) are known to activate cAMP via the 

to the cAMP or cGMP concentration in 

separately. 

action of the enzyme adenylate cyclase 

the sample. Due to a novel acetylation 

which converts ATP to cAMP. 

procedure 1-100 fmol cAMP or 0.1-10 


cAMP Immunoassay Kit 100 assays PK-CA577-K371 

cGMP Immunoassay Kit 100 assays PK-CA577-K372 

Beta-Secretase Fluorometric Assay Kit 100 assays PK-CA577-K360 

Beta-Secretase Inhibitor I 1 mg PK-CA577-7501-1 

Beta-Secretase Inhibitor II 1 mg PK-CA577-7502-1 


24 


1.9 Cell Stress and Metabolism Kits 


Applications 

GST activity measurement and quantitation 

of GST-tagged fusion proteins 

SOD assays 

Determination of the concentration of 

NO metabolites (nitrate/nitrite) 

HAT activity measurement 

Metabolic studies and analysis of 

mutagenic, carcinogenic, and toxic 

effects 

DNA damage detection and 

quantification 

Analysis of the cell redox state 

Features 

Highly sensitive and reproducible 

detection/measurement 

Ready-to-use, convenient assays 

Fluorometric and Colorimetric GST 

Assay Kits 

Glutathione (GSH) is the major intracellular, 

low-molecular-weight thiol and plays 

a critical role in cellular defense against 

oxidative stress in tissues and cells. Glutathione 

S-transferase (GST) is involved 

in detoxification of xenobiotics mediating 

conjugation of glutathione with those 

compounds. 

The Fluorometric and Colorimetric GST 

Assays can detect GST activity in crude 

cell lysates or purified protein fractions 

and the kits also quantitate GST-tagged 

fusion proteins. They are based upon the 

GST-catalyzed reaction between GSH 

and the GST substrate CDNB (Colorimetric 

Kit) or MCB (Fluorometric Kit). 

CDNB has the broadest range of isozyme 

detectability (a-, µ-, f-, and other GST 

isoforms). Under certain conditions, the 

interaction between glutathione and 

Xanthine 

O 2 

Fig. 8: Principle of the SOD Assay Kit. 

- 

O 3 S 

CDNB is totally dependent on the presence 

of active GST. The detection limit 

is approximately 10 ng active GST/assay. 

The free form of MCB is almost nonfluorescent, 

whereas the dye fluoresces 

blue (Ex/Em = 380/461 nm) when reacting 

with glutathione. GST catalyzes the 

MCB-glutathione reaction, and the fluorescence 

levels are proportional to the 

amount of GST present in the reaction. 

Therefore, the GST level in samples can 

be easily detected using a fluorometer or 

a 96-well fluorometric plate reader. 

SOD Assay Kit 

2O 2 

Xanthine oxidase 

- 

SO 3 

N 

- 

N 

Uric acid 

-. 

2O O 3 S 

2 N+ 

H 2 O N 

2 

Superoxide dismutase (SOD) is one of 

the most important antioxidative enzymes. 

The sensitive SOD Assay Kit 

utilizes WST-1 that produces a watersoluble 

formazan dye upon reduction 

with the superoxide anion. The rate of 

the reduction with a superoxide anion is 

linearly related to the xanthine oxidase 

(XOD) activity and is inhibited by SOD 

(Fig. 8). Therefore, the inhibition activity 

of SOD can be determined by a colorimetric 

method. 

Fluorometric and Colorimetric 

Nitric Oxide Kits 

Nitric oxide (NO) plays an important role 

in neurotransmission, vascular regulation, 

immune responses, and apoptosis. 

The Fluorometric and Colorimetric Nitric 

Oxide Assay Kits provide an accurate and 

convenient tool for total nitrate/nitrite 

concentration determination in a simple 

two-step process that can be used as a 

quantitative measure of NO production. 

The first step is the conversion of nitrate 

to nitrite utilizing nitrate reductase. The 

second step involves the addition of the 

- 

SO 3 

N 

SOD O 2 + H 2 O 2 

Inhibition reaction 

N 

N 

H 

N 

I 

NO 2 

I 

Na + 

NO 2 

WST-1 Formazan 

WST-1 

fluorometric reagent DAN followed by 

NaOH which converts nitrite into a fluorescent 

compound (Fluorometric Nitric 

Oxide Kits) or the addition of Griess Reagent 

which converts nitrite into a deep 

purple azo compound (Colorimetric 

Nitric Oxide Kits). Measurement of the 

fluorescent compound reaction product 

or azo chromophore accurately determines 

the total nitric oxide production. 

The Fluorometric Assay Kit has been 

validated with culture media, plasma, 

and tissue homogenates. 

HAT Activity Assay Kit 

Histone acetyltransferases (HATs) have 

been implicated in playing a crucial role 

in various cellular functions such as gene 

transcription, cell differentiation, and 

proliferation. 

The colorimetric HAT Activity Assay Kit 

offers a convenient, non-radioactive 

system for rapid and sensitive spectrophotometric 

detection of HAT activity in 

mammalian samples. The detection can 

be continuous and is suitable for kinetic 

studies. 

DNA Damage Detection Kit 

Different physiological and environmental 

factors such as chemically induced damage 

and cell aging cause DNA lesions. 

AP sites (apurinic/apyrimidinic sites) are 

one of the major types of DNA lesions 

formed during the process of base excision 

and repair of oxidized, deaminated, 

or alkylated bases. It has been estimated 

that about 2x10 5 base lesions are generated 

per cell per day. For this reason, the 

level of AP sites in cells can be a good 

indicator of DNA lesions and repairs. 

The PromoKine DNA Damage Detection 

Kit utilizes the ARP (Aldehyde Reactive 

Probe) reagent that reacts specifically 

with the aldehyde group in the open 

ring form of the AP sites. After treating 

DNA containing AP sites with ARP 

reagents, the AP sites are tagged with 

biotin residues, which can be quantified 

using an avidin-biotin assay followed by 

colorimetric detection. The kit provides 

the necessary reagents for convenient 

determination of abasic sites in purified 

DNA samples in a 96-well plate format.


NAD/NADH and NADP/NADPH 

Quantitation 

of ATP. A variety of genetic diseases 

can affect ATP formation in the mitochondria. 

There are a number of com- 

phosphatases, phosphorylases and kinases. 

ADP is stored in platelets and can 

be released to interact with a variety of 

mercially available ATP assays available 

purinergic receptors. ADP levels regulate 

PromoKine’s NAD/NADH and NADP/ 

NADPH Quantitation Kits provide a convenient 

tool for sensitive detection of the 

intracellular nucleotides NAD, NADH, 

that can detect very low amounts of ATP 

by measuring luminescence (PromoKine 

Kit PK-CA577-254-200, for example). 

However, these kits require specialized 

several enzymes involved in intermediary 

metabolism. ADP conversion to ATP 

primarily occurs within the mitochondrion 

and chloroplast, although several 


NADP, and NADPH along with the de- 

luminescence instrumentation and utilize 

such processes occur in the cytoplasm. 

termination of their ratios. They can be 

luciferase which can be difficult to main- 

Conventionally, ADP levels have been 

used for studies of energy transforma- 

tain in an active form. 

measured by luciferase/luciferin medi- 

tion and the determination of the redox 

PromoKine’s new Colorimetric & Fluoro- 

ated assays after ADP is converted to 

state of cells or tissue. 

metric ATP Quantitation Kit is designed 

ATP. However, the luciferase system is 

The kits have been designed to specifical- 

to be robust and has a simple straight- 

unstable and luminescence equipment 

ly recognize NAD/NADH only or NADP/ 

forward method which utilizes the phos- 

is not generally available in most labo- 

NADPH only in an enzyme cycling reac- 

phorylation of glycerol to generate a 

ratories. 

tion that significantly increases detection 

product that is easily quantified by colo- 

PromoKine’s new Fluorometric & Colori- 

sensitivity. Purification of NAD/NADH or 

rimetric (λmax = 570 nm) or fluoromet- 

metric Quantitation ADP Kit provides a 

NADP/NADPH from sample mixes is not 

ric (Ex/Em = 535/587 nm) methods. The 

convenient colorimetric and fluorometric 

required. 

assay can detect as low as 50 picomole 

method to measure the ADP level. In 

Fluorometric & Colorimetric ATP 

Quantitation Kit 

(1 µM) of ATP in various samples. 

Fluorometric & Colorimetric ADP 


this assay, ADP is converted to ATP and 

pyruvate. The generated pyruvate can be 

quantified colorimetrically (λmax = 570 

nm) or fluorometrically (Ex/Em 535/587 

ATP that is formed exclusively in the mi- 

nm). The assay is simple, sensitive, stable 

tochondria is the major energy currency 

ADP is a product of ATP dephospho- 

and high-throughput adaptable. It can 

of living systems and virtually all energy 

rylation and can be rephosphorylated 

detect as low as 1 µM ADP in biological 

requiring processes utilize the chemical 

back to ATP. Dephosphorylation and 

samples. 

energy stored in the phosphate bond 

rephosphorylation occur via various 


Colorimetric GST Assay Kit 100 assays PK-CA577-K263 

Fluorometric GST Assay Kit 100 assays PK-CA577-K260 

HAT Activity Assay Kit 100 assays PK-CA577-K332 

Colorimetric Nitric Oxide Assay Kit 2 x 96 assays PK-CA577-K262 

Fluorometric Nitric Oxide Assay Kit 2 x 96 assays PK-CA577-K252 

SOD Assay Kit 100 assays PK-CA577-K335 

DNA Damage Detection Kit 25 assays PK-CA577-K253 

NAD/NADH Quantitation Kit 100 assays PK-CA577-K337 

NADP/NADPH Quantitation Kit 100 assays PK-CA577-K347 

Fluorometric & Colorimetric ATP 


Fluorometric & Colorimetric ADP 


100 assays PK-CA577-K354 


HAT protein (P/CAF, active) 100 µg PK-RP577-1137-100 

GST Inhibitor-1 (Cibacron Blue F3G-A) 1 g PK-CA577-1555-1 

GST Inhibitor-2 (ethacrynic acid) 1 g PK-CA577-1556-1 

Oxidized Glutathione (GSSG) 1 g PK-CA577-1241-1 

Reduced Glutathione (GSSG) 1 g PK-CA577-1242-1 

GST protein (active) 1 mg PK-RP577-1243-1 

Superoxide Dismutase (SOD, human) 100 µg PK-RP577-4802-100 


26 


1.10 Cell Fractionation and Protein Extraction Kits 

Applications 

Nuclear/Cytosol Fractionation Kit 

estimation of protein sizes, and verification 

of Western transfer efficiency onto 

Cellular protein extraction from 

The Nuclear/Cytosol Fractionation Kit 

membranes (PVDF, nylon, or nitrocellu- 

mammalian cells 

provides a complete system that enables 

lose). They show excellent stability with 


Integral membrane protein extraction 

Preparation of highly enriched cellular 

fractions (mitochondria, cytosol, 

nucleus) from mammalian cells 

the separation of nuclear and cytoplasmic 

fractions of mammalian cells and tissues 

with little or no cross-contamination, 

maintaining the nuclear and cytoplasmic 

sharp bands, and allow easy identification 

as bands of different colors are used. 

Each Prestained RTU Protein Ladder is 

sufficient for 100-200 mini gels. 

Features 

Convenient and efficient cell fraction- 

compartments separate and intact. 

Mitochondria/Cytosol 

Fractionation Kit 

The Prestained RTU Protein Ladder I 

contains 9 pink-stained protein markers 

covering the range from 10 to 175 kDa 

and three blue-stained reference bands 

ation and protein extraction 

at 10, 40 and 90 kDa. 

Easy protocols 

The Mitochondria/Cytosol Fractionation 

The Prestained RTU Protein Ladder II 

Proteins and cell fractions can be used 

Kit allows isolation of a highly enriched 

is a three-color protein standard cover- 

for many downstream applications 

mitochondrial fraction from the cyto- 

ing a broad range (10-245 kDa) with 

Mammalian Whole Cell Protein 

Extraction Kit 

sol of mammalian cells in a simple and 

staightforward procedure without ultracentrifugation 

and toxic chemicals. 

ten blue-stained protein bands and 

two reference bands at 25 kDa (green) 

and 75 kDa (red). 

The Mammalian Whole Cell Protein Ex- 

Cytosol/Particulate Fractionation Kit 

traction Kit has been optimized for complete 

extraction of all cellular proteins. 

The Cytosol/Particulate Fractionation 

It contains an optimized lysis buffer and 

Kit provides an easy and convenient sys- 

a protease inhibitor cocktail as well as 

tem for rapid separation of cytosol and 

other reagents for convenient extraction 

particulate fractions. The separated frac- 

of mammalian proteins from tissues and 

tions are compatible with downstream 

cells under non-denaturing conditions. 

applications such as assaying cellular 

The entire procedure takes less than 20 

distributions of small molecules and pro- 

minutes, and the proteins can be used for 

teins or examining signal transduction 

many downstream applications such as 

pathways. 

gel electrophoresis, Western blotting, etc. 

Complete Cell Fractionation Kit 

Membrane Protein Extraction Kit 

The Complete Cell Fractionation Kit is 

The Membrane Protein Extraction Kit 

designed for serial sample preparation of 

provides an easy and complete protocol 

four distinct protein fractions, including 

for isolating integral membrane proteins 

cytosol, particulate, cytoskeleton, and 

efficiently from cultured mammalian 

nuclear fractions from one sample. 

cells. The kit has been designed to not 

The prepared fractions are suitable for 

only extract the total cellular membrane 

many downstream assays such as two- 

proteins but also to specifically purify the 

dimensional gel electrophoresis, protein 

plasma membrane proteins. The pro- 

immunoblotting, gel-shift assays, en- 

cedure offers consistent yield and high 

zyme activity assays, reporter assays, 

purity (>90%). Membrane proteins pre- 

and more. 

pared using the kit can be utilized in a 

variety of applications such as Western 

Prestained RTU Protein Ladders 

blotting, 2-D gels, enzyme analyses, 

etc. The entire procedure takes less than 

PromoKine’s Prestained RTU Protein 

1 hour. 

Ladders are very cost-effective and 

supplied ready-to-use for direct load- 

Fig. 9: PromoKine’s RTU Protein Ladder 

ing onto gels. They can be used for 

I (above) and RTU Protein Ladder II (be- 

monitoring protein separation during 

low) separated on different SDS poly- 

SDS-polyacrylamide gel electrophoresis, 

acrylamide gels.


GelQuick Protein Gel Stain/ 

Destaining Kit 

the sensitivity of band detection. 

PromoKine’s GelQuick Gel Stain/Destain- 

After a total of 20 minutes visualization 

of as little as 5 ng of protein on a water- 

ing Kit provides a fast and sensitive 

clear background can be seen. 

Traditional staining and destaining 

method for SDS-PAGE gel staining and 

The kit contains sufficient solutions 

methods for SDS-PAGE protein gels are 

known to take several hours and also leave 

a high blue background which reduces 

destaining. The procedure only takes 5 

minutes for gel staining and 15 minutes 

for destaining. 

to stain and destain up to 40 pieces of 

protein mini gels. 



Mammalian Whole Cell Protein Extraction 

Kit 


Membrane Protein Extraction Kit 50 assays PK-CA577-K268 

Nuclear/Cytosol Fractionation Kit 

Mitochondria/Cytosol Fractionation Kit 

25 assays 

100 assays 

25 assays 

100 assays 

PK-CA577-K266-25 

PK-CA577-K266-100 

PK-CA577-K256-25 

PK-CA577-K256-100 

Cytosol/Particulate Fractionation Kit 50 assays PK-CA577-K267 

Complete Cell Fractionation Kit 50 assays PK-CA577-K270 

Prestained RTU Protein Ladder I 2 x 0.25 ml PK-MB713-001P 

Prestained RTU Protein Ladder II 0.5 ml PK-MB713-002P 

GelQuick Protein Gel Stain/Destaining Kit For 40 minigels PK-MB577-K901 


28 


1.11 Nucleic Acid Isolation Kits 

Applications 

in 10-30 minutes with DNA recovery of 

Total RNA Isolation Kit I 

70-100 % - permitting efficient isolation 

DNA/RNA isolation 

of genomic DNA from a large number of 

The Total RNA Isolation Kit I is a com- 

Various molecular genetic applications 

samples of small or large volumes. The 

plete kit with ready-to-use reagents for 


Features 

Fast, simple, and reproducible 

isolated DNA can be used without additional 

purification for Southern blotting, 

dot blot hybridization, molecular cloning, 

RFLP, PCR, and other molecular biology 

the isolation of total RNA from samples 

of human, animal, plant, yeast, bacterial, 

or viral origin. It is based on disrupting 

cells in guanidine thiocyanate/detergent 

procedures 

applications. 

solution, followed by organic extraction 

Optimal yields 

and alcohol precipitation of the RNA, 

Highly pure nucleic acids suitable for 

The separately available Plant DNA 

and the kit allows simultaneous process- 

many applications 

Isolation Buffer binds the reactive poly- 

ing of a large number of samples. 

Improved safety due to replacement 

of hazardous compounds 

phenolic and polysaccharide contaminants 

present in plant tissue. The also 

Total RNA Isolation Kit II 

separately available Blood DNA Isola- 

DNA Isolation Kit 

tion Buffer has been designed for the 

In the Total RNA Isolation Kit II, chlo- 

isolation of genomic DNA from frozen 

roform is replaced by 1-Bromo-3-chlo- 

The easy-to-use DNA Isolation Kit can 

or fresh blood. Both isolation buffers are 

ropropane (BCP) - thus reducing toxic 

be used for extracting DNA from aga- 

intended for use with the PromoKine 

material handling without any adverse 

rose gels, isolating plasmid DNA from 

Genomic DNA Isolation Kit II. 

effects on the quality of isolated RNA, 

mini-preps, and for concentrating DNA 

without ethanol precipitation. The puri- 

Mitochondrial DNA Isolation Kit 

DNA, or proteins. 

The resulting RNA is suitable for the iso- 

fication procedures are quick and yield 

lation of Poly A + RNA, Northern blotting, 

pure DNA that can be used in ligations, 

The Mitochondrial DNA Extraction Kit 

dot blotting, in vitro translation, cloning, 

PCR, cloning, probe construction, se- 

provides a convenient tool for isolating 

RT-PCR, RNase protection assays, and 

quencing, etc. 

mtDNA from a variety of cells and tis- 

other analytical procedures. 

sues with high yield and purity, without 

Genomic DNA Isolation Kit I 

contamination from genomic DNA. The 

The RBC Lysis Solution selectively lyses 

purified mtDNA can be used for a variety 

erythrocytes and is intended for use in 

The Genomic DNA Isolation Kit I pro- 

of studies such as enzyme manipulation, 

the isolation of leukocytes from whole 

vides a simple and convenient procedure 

Southern blotting, cloning, PCR analysis, 

blood. It can be used with the kits 

for rapid isolation of genomic DNA from 

and amplifications. 

PK-MB20-600-50, PK-MB20-400-100, 

mammalian cells and tissue samples with 

high yield and purity. The novel method 

requires less than 90 minutes to prepare 

PCR Purification Kit 

and PK-MB20-410-100. 

FluoDNA Gel Stain 

highly pure genomic DNA that is free 

The PromoKine PCR Purification Kit 

of protein and RNA and is suitable for a 

has been designed for the work-up of 

PromoKine’s FluoDNA Gel Stain is a 

variety of applications such as PCR, DNA 

PCR reactions (removal of primer dim- 

non-mutagenic fluorescent reagent that 

hybridization, cloning, Southern blotting, 

ers, primers, nucleotides, proteins, salt, 

produces instant visualization of DNA 

and array-based experiments. 

agarose, ethidium bromide, and other 

bands upon Blue Light or UV illumina- 

Genomic DNA Isolation Kit II 

impurities). The preparation is based on 

a silica-membrane technology for bind- 

tion of agarose gels. 

Supplied in 6x DNA Loading Buffer, it 

ing DNA in high-salt and elution in low- 

is used to prepare DNA samples and 

The Genomic DNA Isolation Kit II con- 

salt buffer. The kit provides a simple and 

markers for loading onto agarose or 

tains a non-organic and ready-to-use 

efficient way to purify linear or circular 

polyacrylamide gels. FluoDNA Gel Stain 

reagent for the isolation of genomic 

DNA and is optimized for working with 

is a highly sensitive stain available for 

DNA from samples of human, animal, 

DNA amounts of up to 50 µg. It requires 

detecting double-stranded DNA (ds- 

plant, yeast, bacterial, and viral origin. 

no organic extractions or precipitation 

DNA) and contains three tracking dyes 

There is no phenol step included. The 

and guarantees high and stable recovery 

(Bromophenol Blue, Xylene Cyanol FF, 

procedure is fast and can be completed 

rates. 

and Orange G) for monitoring the DNA


migration during the electrophoresis 

process. FluoDNA Gel Stain is an eco- 

to-use, sufficient for 100-200 mini gels. 

The RTU DNA Ladder I displays 11 sharp 

DNA Mass 

(ng/5ml) 

Base 

Pairs 

DNA Mass 

(ng/5ml) 

Base 

Pairs 

nomic, safe and non-hazardous alter- 

bands ranging from 100 to 1,500 base 

native to ethidum bromide. It provides 

pairs. Two bands (100 and 1,500 base 

high sensitivity, shows a low background 

and causes less DNA damage that can 

improve efficiency in downstream applications 

such as gene cloning. 

pairs) have an increased intensity and 

serve as reference points. The ladder 

comes ready-to-use containing Orange G 

and Xylene Cyanol FF as tracking dyes. 


The RTU DNA Ladder II includes 13 frag- 

RTU DNA Ladders 

ments ranging from 250 to 10,000 bp 

with two bands (1,000 and 3,000 base 

PromoKine’s RTU DNA Ladders are suit- 

pairs) which have an increased inten- 

able for use as molecular weight standards 

sity to serve as reference points. It comes 

for agarose gel electrophoresis. They are 

ready-to-use containing Bromphenol Blue 

very cost-effective and supplied ready- 

as tracking dye. 

Fig. 10: PromoKine’s RTU DNA 

Ladder I (left) and RTU DNA Ladder II 

(right) separated on 1.7% and 1.0% 

TAE agarose gels, respectively. 


DNA Isolation Kit 150 - 300 isolations PK-MB20-200-300 

Genomic DNA Isolation Kit I 50 assays PK-MB577-K281 

Genomic DNA Isolation Kit II 50 ml PK-MB20-600-50 

Mitochondrial DNA Isolation Kit 50 isolations PK-CA577-K280 

Plant DNA Isolation Buffer 10 ml PK-MB01-893 

Blood DNA Isolation Buffer 100 ml PK-MB01-894 

RNA Isolation Kit I 100 ml PK-MB20-400-100 

RNA Isolation Kit II 100 ml PK-MB20-410-100 

RBC Lysis Solution 100 ml PK-MB01-888-1 

PCR Purification Kit 

50 preparations 

250 preparations 

PK-MB20-300-50 

PK-MB20-300-250 

RTU DNA Ladder I (100 bp) 0.5 ml PK-MB713-001N 

RTU DNA Ladder II (1000 bp) 0.5 ml PK-MB713-002N 

FluoDNA Gel Stain 1 ml PK-MB713-003 


Apoptosis 

2 

Apoptosis, or programmed cell death, is a sophisticated process 

that involves an intricate series of biochemical events. Specific 

cell death signals are transduced by different biomolecules such 

as caspases, which initiate an apoptotic cascade. This process 

culminates in multiple events such as degradation of cellular 

proteins and chromosomal DNA, mitochondrial disruption, and 

changes in plasma membrane integrity.

32 


2 

Apoptosis 

2.1 DNA Fragmentation Detection Kits ...... 33 

2.2 Mitochondrial Apoptosis Staining Kit. .... 34 

2.3 Cytochrome c Apoptosis Detection Kit ... 34 

2.4 Glutathione Detection Kits. ............ 35 

2.5 Annexin V Detection Kits ............. 36 

2.6 Caspase Kits ....................... 38 

2.7 Cathepsin/Calpain Detection Kits ....... 42 

2.8 Kinase Detection Kits ................ 43 

2.9 Apoptotic and Necrotic Cell Detection. ... 44 

2.10 Apoptotic Cell Isolation Kit ............ 45 

2.11 Apoptosis Inducers .................. 45

2 Apoptosis 33 

2.1 DNA Fragmentation Detection Kits 

Applications 

add Br-dUTP (bromolated deoxyuridine 

mentation in tissue sections and fixed 

triphosphate nucleotides) to the frag- 

cell preparations. It includes positive 

Detecting fragmented 

mented DNA strands. Br-dUTP is more 

and negative control slides and utilizes 

nucleosomal DNA 

readily incorporated into DNA strand 

Br-dUTP, biotin-labeled anti-BrdU anti- 

Screening for apoptotic cells 

breaks than other larger ligands (e.g., 

bodies, and HRP-streptavidin conjugate. 

Fluorescence microscopy 

fluorescein, biotin, or digoxigenin). The 

Immunohistochemical staining results 

Flow cytometry 

incorporated Br-dUTP is subsequently 

can be analyzed with a microscope. 

Western blotting 

Features 

labeled with a green (Kit I) or a redfluorescent 

(Kit II) anti-BrdU monoclonal 

antibody resulting in a very bright signal. 

Apoptotic DNA Ladder 

Detection Kit I 

The labeled DNA can then be observed 

High sensitivity 

by fluorescence microscopy or analyzed 

The Apoptotic DNA Ladder Detection 



Compatible with standard lab 

by flow cytometry. The DNA Fragmentation 

Kit II works also very well with GFP 

transfected cells. 

Kit I provides an easy and sensitive 

method for detecting DNA fragmentation 

in apoptotic cells. DNA preparation 

2 Apoptosis 

equipment 

Internucleosomal DNA fragmentation is 

Fluorometric DNA Fragmentation 

Detection Kit III (F-dUTP) 

is done in less than 90 minutes without 

the need for extraction or separation 

columns. Afterwards, the DNA frag- 

a key sign of apoptosis in mammalian 

ments can easily be visualized by aga- 

cells. PromoKine offers four different kits 

The Fluorometric DNA Fragmentation 

rose gel electrophoresis. This kit allows 

for analyzing DNA fragmentation which 

Detection Kit III is a modified TUNEL as- 

the efficient recovery of small DNA frag- 

are based on fluorometric or colorimetric 

say. It provides all reagents required for 

ments ensuring high sensitivity. 

assays. Since all of the kits are designed 

the specific detection of apoptotic cells in 

The Apoptotic DNA Ladder Detection 

for use with various multiwell formats, 

a cell population, in tissue sections, and 

Kit I plus additionally includes a highly 

they are also suited for automation. 

in fixed cell preps. The kit utilizes termi- 

sensitive DNA staining dye for improved 

Fluorometric DNA Fragmentation 

Detection Kits I and II (BrdU) 

The PromoKine DNA Fragmentation 

nal deoxynucleotidyl transferase (TdT) to 

catalyze the incorporation of fluorescein- 

12-dUTP at the free 3’-hydroxyl ends of 

the fragmented DNA. The fluoresceinlabeled 

DNA can then be observed by 

visualization of DNA fragmentation by 

agarose gel electrophoresis. 

Apoptotic DNA Ladder 

Detection Kit II 

Detection Kits I and II are based on the 

fluorescence microscopy or analyzed by 

TUNEL method and identify DNA strand 

flow cytometry. 

Using the Apoptotic DNA Ladder Extrac- 

breaks that occur during apoptosis. The 

kits provide all required components - 

including positive and negative control 

Colorimetric DNA Fragmentation 

Detection Kit (IHC) 

tion Kit II allows the selective isolation 

of DNA ladders without interference of 

regular genomic DNA. The kit can detect 

cells and tissue slides - for convenient 

apoptotic DNA ladders from tissues and 

detection of DNA fragmentation by fluo- 

The ready-to-use, TUNEL-based Colo- 

cells with an apoptotic cell population 

rescence microscopy or flow cytometry. 

rimetric DNA Fragmentation Detection 

of as little as 5%. The kit specifically ex- 

The TUNEL assay employs a terminal 

Kit (IHC) has been specifically designed 

tracts laddered DNA, not genomic DNA. 

deoxynucleotidyl transferase (TdT) to 

for expedient detection of DNA frag- 


Fluorometric DNA Fragmentation Detection Kit I (BrdU) 60 assays PK-CA577-K401 

Fluorometric DNA Fragmentation Detection Kit II (BrdU) 60 assays PK-CA577-K404 

Fluorometric DNA Fragmentation Detection Kit III (F-dUTP) 50 assays PK-CA577-K402 

Colorimetric DNA Fragmentation Detection Kit (IHC) 50 assays PK-CA577-K403 

Apoptotic DNA Ladder Detection Kit I 50 assays PK-CA577-K120 

Apoptotic DNA Ladder Detection Kit I plus 50 assays PK-CA577-K130 

Apoptotic DNA Ladder Detection Kit II 50 assays PK-CA577-K170 


34 

2 Apoptosis 

2.2 Mitochondrial Apoptosis Staining Kit 

Applications 

Disruption of mitochondrial transmem- 

apoptotic cells, a shift in mitochondrial 

brane potential is one of the earliest 

transmembrane potential prevents the 

Screening for early apoptotic cells 

intracellular events that occurs upon the 

dye from accumulating in the mitochon- 


induction of apoptosis. The Mitochon- 

dria. Instead, it remains in the cytoplasm 


drial Apoptosis Staining Kit provides a 

in its monomer form - fluorescing green. 

Features 

simple, fluorescent-based in vitro assay 

for detecting the mitochondrial changes 

The fluorescent signals can easily be 

detected by fluorescence microscopy or 

in apoptosis, and the kit distinguishes 

flow cytometry using common filters 

Excellent sensitivity due to high 

between healthy and apoptotic cells. 

and channels (FITC, rhodamine, PI). The 

fluorescence intensity 

The assay utilizes the novel cationic 

assay takes approximately 20 minutes, is 


dye, MitoCapture TM 

that fluoresces 

highly sensitive, and detects apoptosis 


differently in healthy versus apoptotic 

reliably in living cells. 

2 Apoptosis 




cells. In healthy cells, MitoCapture TM 

accumulates and aggregates in the mitochondria 

- fluorescing brightly red. In 

MitoCapture Incubation Buffer, a component 

of the kit, is also available separately. 


Mitochondrial Apoptosis Staining Kit 


25 assays 

100 assays 

PK-CA577-K250-25 

PK-CA577-K250-100 

2.3 Cytochrome c Apoptosis Detection Kit 

Applications 


Analysis of apoptotic signal cascades 

Features 



No toxic chemicals necessary 

Cytochrome c has been shown to play 

an important role in apoptosis. The protein 

is located between the inner and 

outer mitochondrial membranes. An 

apoptotic stimulus triggers the release 

of cytochrome c into the cytosol where 

it binds to Apaf-1. The cytochrome 

c/Apaf-1 complex activates caspase-9 

which then activates caspase-3 and 

other downstream caspases of the apoptotic 

cascade. 

The PromoKine Cytochrome c Apoptosis 

Detection Kit provides an effective means 

for detecting cytochrome c translocation 

from mitochondria into the cytosol during 

apoptosis. The kit is easy to use and 

provides unique reagents for separating 

a highly enriched mitochondria fraction 

from the cytosol. Cytochrome c released 

from mitochondria into the cytosol is 

then detected by Western blotting using 

the cytochrome c antibody provided in 

the kit. The procedure is very simple and 

straightforward. No ultracentrifugation 

is required, and no toxic chemicals are 

involved. 


Cytochrome c Apoptosis Detection Kit 100 assays PK-CA577-K257 



2.4 Glutathione Detection Kits 

Applications 


Glutathione detection 

Quantitation of glutathione 

reductase activity 

Features 

Non-radioactive, highly sensitive 

method 



equipment 

Fluorometric Glutathione 

Detection Kit 

Diminished glutathione (GSH) levels 

occur at the early stages of apoptosis. 

The Fluorometric Glutathione Detection 

Kit provides a simple in vitro assay for 

detecting the GSH changes in apoptosis 

and other pathological processes. The 

assay utilizes a dye (monochlorobimane, 

MCB) that has a high affinity for glutathione. 

The unbound dye is almost nonfluorescent, 

whereas the dye fluoresces 

blue (excitation = 380 nm; emission = 461 

nm) when bound to reduced or oxidized 

glutathione. Consequently, the decrease 

in glutathione level in apoptotic cells can 

be easily detected using a fluorometer or 

a 96-well fluorometric plate reader. 

Colorimetric Glutathione 

Detection Kit 

The Colorimetric Glutathione Detection 

Kit provides a convenient colorimetric 

method for analyzing either total 

glutathione or only the reduced form 

of glutathione using a microtiter plate 

reader. The assay utilizes the glutathione 

recycling system with DTNB and glutathione 

reductase. GSH is regenerated 

from GSSG (oxidized glutathione) by 

glutathione reductase and reacts again 

with DTNB to produce yellow 2-nitro- 

5-thiobenzoic acid that can be detected 

spectrophotometrically at 412 nm (Fig. 

1). This recycling reaction dramatically 

improves the sensitivity of total glutathione 

detection. The kit can quantify total 

glutathione from 1-100 ng/well. For detecting 

lower glutathione concentrations, 

DTNB 

PromoKine’s Colorimetric Glutathione 

Reductase Activity Kit has been designed 

for measuring glutathione reductase 

(GR) activity in biological samples. It 

is highly sensitive and well suited for 

high-throughput. The assay is based on 

the GR-catalysed reduction of GSSG to 

GSH, which reacts with 5,5′-Dithiobis(2- 

nitrobenzoic acid) (DTNB) to generate 

the yellow TNB 2- . The kit can detect 

0.1 – 40 mU/ml GR in various samples. 

2-Nitro-5-thiobenzoic 

acid 

such as in blood samples, increasing the 

reaction time significantly improves the 

signal. The kit can also specifically detect 

the reduced form of glutathione (GSH) 

when glutathione reductase is omitted 

from the reaction mixture. 

Colorimetric Glutathione 

Reductase Activity Kit 

2GSH 

GSSG 

Fig. 1: Principle of the Colorimetric Glutathione Detection Kit. 

Glutathione 

reductase 

+ NADPH 

2 Apoptosis 


Fluorometric Glutathione Detection Kit 100 assays PK-CA577-K251 

Colorimetric Glutathione Detection Kit 100 assays PK-CA577-K261 

Colorimetric Glutathione Reductase 

Activity Kit 



36 

2 Apoptosis 

2.5 Annexin V Detection Kits 

Applications 

be tagged with EGFP (Enhanced Green 

in the provided binding buffer, apoptotic 

Fluorescent Protein). Since Annexin V 

cells show red fluorescence, necrotic cells 


conjugates and tagged annexins retain 

show green fluorescence, and live cells 

Differentiating apoptotic and 

their high affinity for PS, they are ide- 

show little or no fluorescence. These 

necrotic cells 

ally suited as highly sensitive probes to 

populations can easily be distinguished 

Annexin detection 

identify early apoptotic cells and to de- 

by fluorescence microscopy and flow cy- 


tect apoptosis much earlier than assays 

tometry using the appropriate filters and 


based on DNA fragmentation or loss of 

channels. 

membrane integrity. The stained cells 

Features 

can be evaluated by flow cytometry or 

Individual Annexin V Reagents 

fluorescence microscopy using common 

High fluorescence intensity and 

filter sets and flow cytometer channels. 

PromoKine also offers a wide range of 

2 Apoptosis 

sensitivity 

Many different labels and tags 

available 

Annexin V Detection Kits 

Annexin V reagents separately. For easy 

staining of early apoptotic cells, Annexin 

V conjugated to FITC, PE, Cy3, 


The Annexin V Apoptosis Detection 

Cy5, or biotin is available, as well as An- 


Kits include an Annexin V conjugate or 

nexin V tagged with EGFP. The stained 


tagged Annexin V as well as binding buf- 

cells can be evaluated by flow cytometry 


fer and in some cases Propidium Iodide 

or fluorescence microscopy using com- 


(PI) for differentiating between early 

mon filter sets and flow cytometer chan- 

apoptotic and dead cells. The kits allow 

nels. Biotinylated Annexin V can be de- 

In the early stages of apoptosis, changes 

a one-step staining procedure that takes 

tected in conjunction with conventional 

can be observed on the cell surface. 

only 10 minutes. In addition, the assay 

dye-staining using any streptavidin- or 

Cells lose their phospholipid membrane 

can be performed directly on living cells. 

avidin-dye reagents such as PromoFluor- 

asymmetry and expose phosphatidyl- 

streptavidin/avidin conjugates, fluores- 

serine (PS) on the exterior plasma mem- 

Promokine Annexin V Apoptosis Detec- 

cein-, PE-, or APC-streptavidin as well 

brane. Annexin V is a Ca 2+ -dependent, 

tion Kit plus includes an additional dye, SY- 

as streptavidin/avidin conjugated to 

phospholipid-binding protein with high 

TOX green, that is impermeant to living 

peroxidase, alkaline phosphatase (AP), 

affinity for PS and is very useful for 

and apoptotic cells, but stains necrotic 

b-galactosidase, etc. 

identifying apoptotic cells that expose 

cells with intense green fluorescence 

PS on their surfaces to the extracellular 

by binding to cellular nucleic acids. Af- 

Please visit www.promokine.info or 

environment. Annexin V may be conju- 

ter staining a cell population with e.g., 

see the PromoKine Price List to find our 

gated to biotin or fluorophores such as 

fluorescent Annexin V-Cy3 or Annexin 

complete range of individual Annexin V 

FITC and phycoerythrin (PE). It can also 

V-PE conjugates, and SYTOX Green dye 

reagents. 


Annexin V-FITC Detection Kit 

Annexin V-FITC Detection Kit Plus 

Annexin V-Cy3 Detection Kit 

Annexin V-Cy3 Detection Kit Plus 

Annexin V-Cy5 Detection Kit 

Annexin V-EGFP Detection Kit 


25 assays 

100 assays 

400 assays 

25 assays 

100 assays 

400 assays 

25 assays 

100 assays 

400 assays 

25 assays 

100 assays 

400 assays 

25 assays 

100 assays 

400 assays 

25 assays 

100 assays 

400 assays 

PK-CA577-K101-25 

PK-CA577-K101-100 

PK-CA577-K101-400 

PK-CA577-K201-25 

PK-CA577-K201-100 

PK-CA577-K201-400 

PK-CA577-K102-25 

PK-CA577-K102-100 

PK-CA577-K102-400 

PK-CA577-K202-25 

PK-CA577-K202-100 

PK-CA577-K202-400 

PK-CA577-K103-25 

PK-CA577-K103-100 

PK-CA577-K103-400 

PK-CA577-K104-25 

PK-CA577-K104-100 

PK-CA577-K104-400



Annexin V-PE Detection Kit 

Annexin V-PE Detection Kit Plus 

Annexin V-PE-Cy5 Detection Kit 

Annexin V-Biotin Detection Kit 

Annexin V-FITC Reagent 

Annexin V-Cy3 Reagent 

Annexin V-Cy5 Reagent 

Annexin V-EGFP Reagent 

Annexin V-PE Reagent 

Annexin V-PE-Cy5 Reagent 

Annexin V-Biotin Reagent 

25 assays 

100 assays 

400 assays 

25 assays 

100 assays 

400 assays 

25 assays 

100 assays 

400 assays 

25 assays 

100 assays 

400 assays 

200 assays 

1000 assays 

200 assays 

1,000 assays 

200 assays 

1,000 assays 

200 assays 

1,000 assays 

200 assays 

1,000 assays 

200 assays 

1,000 assays 

100 μg 

1 mg 

PK-CA577-K128-25 

PK-CA577-K128-100 

PK-CA577-K128-400 

PK-CA577-K203-25 

PK-CA577-K203-100 

PK-CA577-K203-400 

PK-CA577-K129-25 

PK-CA577-K129-100 

PK-CA577-K129-400 

PK-CA577-K109-25 

PK-CA577-K109-100 

PK-CA577-K109-400 

PK-CA577-1001-200 

PK-CA577-1001-1000 

PK-CA577-1002-200 

PK-CA577-1002-1000 

PK-CA577-1013-200 

PK-CA577-1013-1000 

PK-CA577-1004-200 

PK-CA577-1004-1000 

PK-CA577-1014-200 

PK-CA577-1014-1000 

PK-CA577-1015-200 

PK-CA577-1015-1000 

PK-CA577-1003-100 

PK-CA577-1003-1000 

Annexin V Unlabeled Reagent 100 μg PK-CA577-1005-100 

1X Annexin V Binding Buffer 100 ml PK-CA577-1035 

10X Annexin V Binding Buffer 100 ml PK-CA577-1006 

2 Apoptosis 


38 

2 Apoptosis 

2.6 Caspase Kits 

Caspases, a family of cysteine aspartic 

Caspase Detection Kits 

Caspase Staining Kits 

acid proteases, are important factors in 

the progression of apoptosis and can be 

Applications 

The fluorometric Caspase Staining Kits 

activated by various harmful physiological 

are the ideal tools for detecting activated 

stimuli such as cytochrome c release due 


caspases in situ in living cells. The kits 

to mitochondrial membrane disruption, 

Flow cytometry and fluorescence 

utilize potent caspase inhibitors conju- 

DNA damage, ER stress, or inducers such 

microscopy 

gated to FITC or rhodamine as fluores- 

as TNF-alpha and FasL. Caspases are nor- 

Caspase detection 

cent markers. The caspase inhibitors 

mally present in the cell as inactive proenzymes. 

During apoptosis, they are acti- 

Features 

are cell-permeable, nontoxic, and bind 

specifically and irreversibly to the acti- 

vated by proteolysis forming a proteolytic 

vated caspases in apoptotic cells. With 

cascade that results in the cleavage of 

High fluorescent intensity and 

the FITC or rhodamine labels, activated 

2 Apoptosis 

distinct proteins that are essential for cell 

viability. 

sensitivity 

Highly specific 

Fast and simple 

caspases in apoptotic cells can be directly 

visualized by fluorescence microscopy, 

analyzed by using flow cytometry, 


or analyzed with a fluorescence plate 


reader. Caspase inhibitors without FITC 


or rhodamine labels are also included in 


the kits as negative controls. 


The components of the kits (e.g. the 

Wash Buffer) are also available separately 

(please see www.promokine.info). 


Green Multi-Caspase Staining Kit 

Green Caspase-2 Staining Kit 





Red Multi-Caspase Staining Kit 

Red Caspase-3 Staining Kit 




25 assays 

100 assays 

25 assays 

100 assays 

25 assays 

100 assays 

25 assays 

100 assays 

25 assays 

100 assays 

25 assays 

100 assays 

25 assays 

100 assays 

25 assays 

100 assays 

25 assays 

100 assays 

25 assays 

100 assays 

PK-CA577-K180-25 

PK-CA577-K180-100 

PK-CA577-K182-25 

PK-CA577-K182-100 

PK-CA577-K183-25 

PK-CA577-K183-100 

PK-CA577-K188-25 

PK-CA577-K188-100 

PK-CA577-K189-25 

PK-CA577-K189-100 

PK-CA577-K172-25 

PK-CA577-K172-100 

PK-CA577-K190-25 

PK-CA577-K190-100 

PK-CA577-K193-25 

PK-CA577-K193-100 

PK-CA577-K198-25 

PK-CA577-K198-100 

PK-CA577-K199-25 

PK-CA577-K199-100 

For a complete list of active recombinant caspases, caspase substrates, inhibitors, and antibodies please visit our website 

www.promokine.info, or see the PromoKine Price List.


Caspase Screening Kit (FC) 

maximum at 488 nm, emission maxi- 

trifluoromethyl coumarin). Active cas- 

mum at 530 nm). 

pases (provided in the kits) cleave the 

The PromoKine Caspase Screening Kit 

(FC) provides a convenient means for 

Caspase Drug Screening Kits 

synthetic substrates to release free AFC 

which can then be quantified using a 

detecting the activation of caspases by 

fluorescence plate reader. Compounds 

flow cytometry in living cells. The assay 

Inhibition of caspases has been shown 

to be screened can be directly added to 

is based on the cleavage of (aspartyl)2- 

to delay apoptosis, implicating a po- 

the reaction, and the level of caspase 

Rhodamine 110 (D2R), a reported sub- 

tential role in drug screening efforts. 

inhibition can then be determined. The 

strate for members of caspase family 

PromoKine provides a full line of Cas- 

assays are simple, straightforward, and 

proteases. The caspase substrate D2R is 

pase Drug Screening Kits for effective 

can be performed directly in microtiter 

nonfluorescent. However, upon cleav- 

screening of caspase inhibitors using 

plates. Assay conditions for each caspase 

age of the substrate by cellular activated 

fluorometric methods. The assays utilize 

have been optimized to obtain the maxi- 

caspases, the released rhodamine 110 

gives rise to fluorescence that can be 

measured by flow cytometry (excitation 

peptide substrates containing consensus 

cleavage sequences specific for each 

caspase, labeled with AFC (7-amino-4- 

mal activity. 

2 Apoptosis 


Caspase Screening Kit (FC) 

25 assays 

100 assays 

PK-CA577-K200-25 

PK-CA577-K200-100 

Caspase-1 Drug Screening Kit 100 assays PK-CA577-K151-100 













40 

2 Apoptosis 

Caspase Quantification Kits 

Applications 

Caspase Fluorometric & 

Colorimetric Assay Kits 

detection of caspase-3 or caspase-7 activity 

in apoptotic samples. Other cas- 

Apoptosis screening 

pases and non-specific proteases are not 

Quantification of caspase activity 

With the Caspase Fluorometric & Colori- 

detected. 

metric Assay Kits caspase activity in mam- 

Features 

malian cells can be quantified by either 

Individual Caspase Reagents 

fluorescence or absorbance in a fast and 

Highly sensitive and specific 

simple procedure. The kits utilize the cas- 

In addition to the complete caspase 

Fast and convenient 

pase substrate (Ac-DEVD) 2 

-R110 that is 

staining and activity kits, PromoKine also 


fluorogenic as well as chromogenic. It 

provides a variety of high quality, puri- 

equipment 

has an extinction coefficient that is 10 

fied active recombinant caspases and 

Suited for automation 

times higher than that of pNA - making 

pro-caspases from human, mouse and 

2 Apoptosis 

Caspase Fluorometric Assay Kits 

The Caspase Fluorometric Assay Kits 

the assay extremely sensitive. 

Caspase Fluorometric Assay 

Kits (HTS) 

rat, as well as caspase substrates and 

inhibitors. Optimized assay buffers and 

other supplementary reagents for setting 

up custom assays are also available sepa- 

are intended for convenient and sensi- 

rately. These reagents allow researchers 

tive quantification of caspase activity 

The Caspase Fluorometric Assay Kits 

to perform numerous caspase assays 

in cell lysates. The kits utilize synthetic 

(HTS) have been designed for economic 

economically. Sets of ready-to-use cas- 

peptides which mimic the optimal sub- 

high-throughput applications. They use 

pase enzymes, caspase substrates, and 

strate for distinct caspases and are la- 

the fluorogenic and chromogenic sub- 

caspase inhibitors provide more conve- 

beled with 7-amino-4-trifluoromethyl 

strate (Ac-DEVD) 2 

-R110 and include 

nience in setting up individual assays to 

coumarin (AFC). Activated caspases in 

specific caspase inhibitors as negative 

evaluate multiple caspases in the same 

apoptotic cells specifically cleave their 

controls. 

experiment. 

synthetic substrates, resulting in the release 

of free AFC which is then quantified 

using a fluorometer. The kits are 

Caspase-3 and Caspase-7 

Immunoassay Kits 

PromoKine’s active caspases are very 

useful for studying enzyme regulation 

and kinetics, screening caspase inhibi- 

fast, straight- 

forward, and adaptable 

tors, determining target substrates, and 

to 96-well plates. 

Caspase-3 plays a key role in the 

as positive controls in caspase activity 

Caspase Colorimetric Assay Kits 

initiation of cellular events during the 

apoptotic process. The PromoKine 

assays and Western blot analyses. 

The caspase substrates come in ready- 

Caspase-3 and Caspase-7 Immunoassay 

to-use form for both fluorometric and 

The Caspase Colorimetric Assay Kits are 

Kits allow quantification of the specific 

colorimetric caspase assays. 

intended for convenient and sensitive 

activity of caspase-3 and -7. Since cas- 

quantification of caspase activity in cell 

pase-3 and -7 share the same target 

Please visit www.promokine.info, or 

lysates. The kits utilize synthetic peptides 

substrate sequence (DEVD), it is difficult 

see the PromoKine Price List to find the 

which mimic the optimal substrate for 

to differentiate the cleavage activity con- 

complete range of PromoKine individual 

distinct caspases and are labeled with 

tributed by these two caspases in vitro. 

caspase reagents. 

p-nitroanilide (pNA). Activated caspases 

Consequently, the assays utilize cas- 

A range of caspase antibodies can be 

in apoptotic cells specifically cleave their 

pase-3 or -7 specific antibodies to cap- 

found in chapter 6. 

synthetic substrates, resulting in the re- 

ture the activated caspase-3 or -7 in cell 

lease of free pNA, which is then quanti- 

lysate. The specific activity of caspase-3 

fied using a spectrophotometer. The kits 

or -7 can then be analyzed using the 

are fast, straightforward, and adaptable 

common caspase-3/7 substrate DEVD- 

to 96-well plates. 

AFC. The assay system ensures specific



Caspase-1 Fluorometric Assay Kit 25, 100, 200, 400 assays PK-CA577-K110-XXX* 



Caspase-4 Fluorometric Assay Kit 25, 100, 200 assays PK-CA577-K126-XXX* 





Caspase-10 Fluorometric Assay Kit 25, 100 assays PK-CA577-K124-XXX* 

Caspase-12 Fluorometric Assay Kit 25, 100 assays PK-CA577-K139-XXX* 

Caspase-1 Colorimetric Assay Kit 25, 100, 200, 400 assays PK-CA577-K111-XXX* 



Caspase-4 Colorimetric Assay Kit 25, 100, 200 assays PK-CA577-K127-XXX* 





Caspase-10 Colorimetric Assay Kit 25, 100 assays PK-CA577-K125-XXX* 

Caspase-3 Fluorometric & Colorimetric Assay Kit 

Caspase-3 Fluorometric Assay Kit (HTS) 

Caspase-8 Fluorometric & Colorimetric Assay Kit 

Caspase-8 Fluorometric Assay Kit (HTS) 

25 assays 

100 assays 

1 ml 

10 ml 

100 m 

25 assays 

100 assays 

1 ml 

10 ml 

100 ml 

PK-CA707-30008-1 

PK-CA707-30008-2 

PK-CA707-30009-1 

PK-CA707-30009-2 

PK-CA707-30009-3 

PK-CA707-30011-1 

PK-CA707-30011-2 

PK-CA707-30012-1 

PK-CA707-30012-2 

PK-CA707-30012-3 

Caspase-3 Immunoassay Kit 100 assays PK-CA577-K163 

Caspase-7 Immunoassay Kit 100 assays PK-CA577-K167 

2 Apoptosis 



www.promokine.info, or see the PromoKine Price List. 

* XXX stands for the respective package size: 25, 100, 200, or 400 tests

42 

2 Apoptosis 

2.7 Cathepsin/Calpain Detection Kits 

Applications 

Cathepsin Activity Assay Kits 

Calpain Activity Assay Kits 


The Cathepsin Activity Assay Kits are 

The activation of calpain is involved in 

Cathepsin and calpain detection 

fluorescence-based and utilize the pre- 

many physiological and pathological 

Features 

ferred substrate sequence for each cathepsin, 

labeled with AFC (amino-4-triflu- 

processes (e.g., apoptosis). The Calpain 

Activity Assay Kit provides a simple and 

oromethyl coumarin). Cell lysates and 

convenient means for analysing calpain 


other samples that contain cathepsins 

activity in apoptotic cells and other sam- 


cleave the synthetic substrate to release 

ples. The assay is based on fluorometric 


free AFC that can be easily quantified us- 

detection and quantitation of the cleav- 


ing a fluorometer or a fluorescence plate 

age of a calpain substrate at 505 nm 

Compatible with common 

reader. The cathepsin assays are easy to 

using a fluorometer or a fluorescence 

2 Apoptosis 

fluorometers/fluorescence 

plate readers 

perform and can be adapted to 96-well 

plate assays. Assay conditons have been 

optimized to obtain the maximal activ- 

plate reader. The kit contains positive 

and negative controls. Comparison of 

the fluorescence intensity from a treated 

Apoptosis can be mediated by mecha- 

ity. Active cathepsin as well as cathepsin 

sample with a normal control allows 

nisms other than the well-studied cas- 

inhibitors, and cathepsin antibodies (see 

determination of the changes in calpain 

pase-mediated cleavage cascade. There 

chapter 6) are also available separately. 

activity. Active calpain as well as calpain 

is growing knowledge that alternative 

inhibitors and calpain antibodies (see 

proteolytic enzymes such as the lyso- 

Please visit www.promokine.info, or see 

chapter 6) are also available separately. 

somal cathepsin proteases may initiate 

the PromoKine Price List to find more 

or propagate proapoptotic signals, but 

cathepsin products. 

Please visit www.promokine.info, or see 

it is currently unclear how cathepsin 


mediates apoptosis. 

calpain products. 


Cathepsin B Activity Assay Kit 100 assays PK-CA577-K140 

Cathepsin D Activity Assay Kit 100 assays PK-CA577-K143 

Cathepsin H Activity Assay Kit 100 assays PK-CA577-K145 

Cathepsin K Activity Assay Kit 100 assays PK-CA577-K141 

Cathepsin L Activity Assay Kit 100 assays PK-CA577-K142 

Cathepsin S Activity Assay Kit 100 assays PK-CA577-K144 

Calpain Activity Assay Kit 100 assays PK-CA577-K240 

Cathepsin B, human (native) 5 µg PK-RP577-1021 

Cathepsin D, human (native) 5 µg PK-RP577-1022 

Cathepsin H, human (native) 5 µg PK-RP577-1023 

Cathepsin L, human, recombinant 5 µg 

100 µg 

PK-RP577-1135-5 

PK-RP577-1135-100 

Cathepsin S, human (native) 5 µg PK-RP577-1025 

Calpain I, human (native) 100 µg PK-RP577-1134 

Cathepsin B & L Substrate Z-FR-R110 5 mg PK-CA707-10209 

Cathepsin Substrate Z-FR-AMC 25 mg PK-CA707-10201 

Calpain Inhibitor Z-LLY-FMK 20 µl (10 mM) PK-CA577-1125-20C 

Cathepsin B&L Inhibitor Z-Phe-Phe-FMK 20 µl (10 mM) PK-CA577-1126-20C 



2.8 Kinase Detection Kits 

Applications 

large numbers of samples. The assay 

Akt Activity Detection Kit 

utilizes an N-terminal c-Jun fusion pro- 


tein bound to Glutathione-Sepharose 

The Akt Activity Detection Kit utilizes 

Kinase detection and activity 

beads to selectively “pull down” JNK 

an Akt-specific antibody to immunopre- 

screening 

from cell lysate. After a washing step to 

cipitate Akt from cell lysate. Akt-specific 

Features 

remove non-specifically bound proteins, 

the kinase reaction is carried out in the 

activity is then detected in a kinase reaction 

using recombinant GSK-3a as 

presence of cold ATP. c-Jun phosphoryla- 

a substrate. Phosphorylation levels of 


tion is then determined by Western blot 

GSK-3a are determined by Western blot 

Easy, accurate, and fast procedure 

analysis using a phospho-c-Jun specific 

analysis using a phospho-GSK-3a spe- 


antibody. 

cific antibody included in the kit. The kit 

equipment 

The activation of protein kinases, such 

as JNK and Akt, plays an important role 

JNK Activity Detection Kit 

The JNK Activity Detection Kit utilizes 

specifically detects Akt1, Akt2, and Akt3 

activities. Other kinase activities are not 

detected. 

Active JNK and Akt kinases as well 

2 Apoptosis 

in apoptosis and other physiological and 

a JNK-specific antibody to immuno- 

as JNK/Akt substrates, activators, in- 

pathological processes. PromoKine pro- 

precipitate JNK from cell lysates. The 

hibitors, and control cell lysates are also 

vides a novel non-radioactive system for 

activity of the JNK is then detected in a 

available separately. 

convenient measurement of JNK and 

kinase reaction using recombinant c-Jun 

Please visit www.promokine.info or see 

Akt activities. 

as substrate. c-Jun phosphorylation is 


JNK Activity Screening Kit 

determined by Western blotting using 

a phospho-c-Jun specific antibody. The 

active kinases that can be used for 

cell analysis and apoptosis research. A 

kit specifically detects JNK activity; other 

range of kinase antibodies can be found 

The JNK Activity Screening Kit can 

kinase activities are not detected. 

in chapter 6. 

screen JNK activity quickly even with 


JNK Activity Screening Kit 40 assays PK-CA577-K430 

JNK Activity Detection Kit 40 assays PK-CA577-K431 

Akt Activity Detection Kit 40 assays PK-CA577-K435 

Akt Inhibitor 1 mg PK-CA577-1701 

Chk2 Inhibitor 1 mg PK-CA577-1702 

c-Jun (1-79)-GST Fusion Protein 100 ug PK-CA577-7001 

GSK-3a-GST Fusion Protein 100 ug PK-CA577-7003 

GSK-3β, human, recombinant 100 ug PK-CA577-7004 

L-JNK Peptide Inhibitor I 50 µl (1 mM) PK-CA577-7011 

L-JNKI1 Negative Control Peptide 50 µl (1 mM) PK-CA577-7021 

JNK Negative Control Cell Lysate 1 mg PK-CA577-7031 

JNK Activated Cell Lysate 1 mg PK-CA577-7032 

Akt Negative Control Cell Lysate 1 mg PK-CA577-7035 

Akt Activated Cell Lysate 1 mg PK-CA577-7036 


For a list of active recombinant kinases, kinase substrates, inhibitors, and antibodies please visit our website 


44 

2 Apoptosis 

2.9 Apoptotic and Necrotic Cell Detection 

Applications 

within the same cell population by flow 

cytometry or fluorescence microscopy. 

Simultaneous detection of apoptotic, 

For tagging apoptotic cells, fluorescein 

necrotic, and healthy cells 

labeled Annexin V is used. Annexin V 


specifically binds to the phosphatidyl- 


serin exposed on the outer membrane of 

apoptotic cells. In order to mark necrotic 

Features 

cells, Ethidium homodimer III (EthD-III) 

is utilized. This fluorescent DNA bind- 

Convenient ready-to-use kit 

ing substance can enter necrotic cells 

Fast procedure 

because of the damaged plasma mem- 

Fig. 2: Apoptotic U937 cells stained 


brane, but not living or apoptotic cells. 

with Annexin V-FITC using PromoKine’s 

2 Apoptosis 

Apoptosis and necrosis, the two major 

processes leading to cell death, show 

Once in the necrotic cell, it binds to cellular 

DNA and fluoresces red. Due to its 

significantly higher fluorescence quan- 

Apoptotic/Necrotic/Healthy Cells Detection 

Kit. 

several distinct differences. One char- 

tum yield, Ethidium homodimer III is 

acteristic of apoptotic cells is the trans- 

superior to propidium iodide or Ethidium 

location of phosphatidylserine from the 

homodimer I. 

inner to the outer side of the plasma 

membrane. This allows phagocytic cells 

to recognize and destroy apoptotic 

Apoptotic/Necrotic/Healthy Cells 

Detection Kit 

cells but can also be used for identifying 

apoptotic cells. Necrotic cells, on 

The Apoptotic/Necrotic/Healthy Cells 

the other hand, are characterized by a 

Detection Kit provides convenient quan- 

loss of membrane integrity. This allows 

tification of apoptotic (green), necrotic 

membrane-impermeable probes to enter 

(red), and healthy cells (blue) within the 

Fig. 3: Apoptotic/Necrotic U937 cell 

and stain them. 

same cell population by flow cytometry 

stained with Ethidium homodimer III 

Apoptotic/Necrotic Cells 

Detection Kit 

or fluorescence microscopy. In addition 

to the fluorescent dyes utilized in the 

Apoptotic/Necrotic Cells Detection Kit, 

(EthD-III) using PromoKine’s Apoptotic/ 

Necrotic/Healthy Cells Detection Kit. 

it also contains a membrane-permeable 

The Apoptotic/Necrotic Cells Detection 

blue fluorescent DNA stain (Hoechst 

Kit provides convenient quantification of 

33342) for quantifying healthy cells. 

apoptotic (green) and necrotic (red) cells 


Apoptotic/Necrotic Cells Detection Kit 50 assays PK-CA707-30017 

Apoptotic/Necrotic/Healthy Cells 

Detection Kit 

50 assays PK-CA707-30018 



2.10 Apoptotic Cell Isolation Kit 

Applications 

dead cells from cell culture or tissue 

MagBeads adhere to the magnet - 

preparations using Annexin V magnetic 

while normal cells stay in suspension. 

Isolation of apoptotic cells 

beads (MagBeads). Since Annexin V is a 

The bound apoptotic cells can then be 

Removal of dead cells from culture 

phospholipid-binding protein with high 

released from the MagBeads using the 

Features 

affinity for phosphatidylserine (PS), it 

binds strongly to the PS that is redistrib- 

Elution Buffer provided in the kit. The 

separated apoptotic cells and normal 

uted from the inner to the outer surface 

cells can be used in a variety of assays to 

Fast, simple, and efficient 

of the plasma membrane during apopto- 

study apoptotic mechanisms and path- 

Complete ready-to-use kit 

sis. Annexin V-biotin first binds to apop- 

ways. The kit has also been successfully 

totic cells. Then, biotin binds to strepta- 

used to remove dead cells from normal 

The PromoKine Apoptotic Cell Isolation 

vidin-MagBeads, which enables the 

cultures. 

Kit provides a simple and efficient means 

separation of apoptotic cells from normal 

A re-usable magnetic separator opti- 

for isolating apoptotic cells or removing 

cells. The apoptotic cells bound to the 

mized for the kit is also available. 

2 Apoptosis 


Apoptotic Cell Isolation Kit 30 isolations PK-CA577-K258 

Magnetic Separator (re-usable) 1 PK-CA577-K1999-1 


2.11 Apoptosis Inducers 

Applications 

Induction of apoptotic processes 

Ideally suited for use with diverse 

apoptosis detection systems 

Features 

High quality 

Ready-to-use 

PromoKine offers a select choice of high 

quality apoptosis inducers in ready-to-use 

form. Some of them are also available in 

a convenient set. The apoptosis inducers 

are well-characterized and effective in 

disrupting mitochondrial transmembrane 

potential and activating caspases as well 

as inducing phosphatidyl-serine (PS) exposure, 

DNA fragmentation, and other 

apoptotic characteristics. All inducers are 

ideally suited for use with the various 

PromoKine apoptosis detection systems. 

PromoKine provides high quality growth 

factors, cytokines, and antibodies that 

are relevant to apoptosis research (See 

chapters 6 and 7). PromoKine also 

provides cell viability and cell proliferation 

assay kits that can be used for 

analyzing apoptotic processes (See 

Chapter 1). 


Actinomycin D (10 mM) 50 µl PK-CA577-1036-50 

Actinomycin D 

5 mg 

50 mg 

PK-CA577-1036-5MG 

PK-CA577-1036-50MG 

Anisomycin 10 mg PK-CA577-1549-10 

Apoptosis Inducer Set 1 kit PK-CA577-K121-5 

Betulinic acid 25 mg PK-CA577-1552-25 

Brefeldin A 5 mg PK-CA577-1560-5 

Camptothecin 


50 mg 

500 mg 

PK-CA577-1039-50MG 

PK-CA577-1039-500MG

46 

2 Apoptosis 


2 Apoptosis 

Camptothecin (2 mM) 1 ml PK-CA577-1039-1 

Colchicine 1 g PK-CA577-1519 

Cycloheximide 1 g PK-CA577-1041-1G 

Cycloheximide (100 mM) 1 ml PK-CA577-1041-1 

Daunorubicin.HCl 

Dexamethasone 

10 mg 

50 mg 

1 g 

10 g 

PK-CA577-1524-10 

PK-CA577-1524-50 

PK-CA577-1042-1G 

PK-CA577-1042-10G 

Dexamethasone (10 mM) 1 ml PK-CA577-1042-1 

Doxorubicin.HCl 5 mg PK-CA577-1527-5 

Etoposide 

25 mg 

250 mg 

PK-CA577-1043-25MG 

PK-CA577-1043-250MG 

Etoposide (10 mM) 100 µl PK-CA577-1043-100 

Genistein 

10 mg 

100 mg 

PK-CA577-1533-10 

PK-CA577-1533-100 

Ionomycin (calcium salt) 5 mg PK-CA577-1566-5 

Ionomycin (free acid) 5 mg PK-CA577-1565-5 

Mitomycin C 

5 mg 

10 mg 

100 mg 

PK-CA578-M1108-5 

PK-CA578-M1108-10 

PK-CA578-M1108-100 

Okadaic acid 25 µg PK-CA577-1543-25 

Phorbol-12-myristate 13-acetate (PMA) 5 mg PK-CA577-1544-5 

Rapamycin 

5 mg 

50 mg 

Rosiglitazone 

5 mg 

100 mg 

Staurosporine 100 µg 

1 mg 

PK-CA577-1568-5 

PK-CA577-1568-50 

PK-CA577-1559-5 

PK-CA577-1559-100 

PK-CA577-1048-010 

PK-CA577-1048-1 

Tamoxifen citrate 1 g PK-CA577-1551 

Thapsigargin 1 mg PK-CA577-1558-1 

Tyrphostin AG 1295 5 mg PK-CA577-1571-5 

Tyrphostin AG 490 5 mg PK-CA577-1570-5 



3 

The availability of new fluorophores has dramatically changed 

the possibilities for the sensitive detection of biomolecules and 

the analysis of their interactions. Improved fluorescent dyes are 

now enabling previously impossible studies of cellular structures 

and processes. PromoKine provides a wide range of highquality 

fluorophores for easy labeling of diverse biomolecules 

including proteins, antibodies, and DNA, as well as complete 

labeling kits and ready-to-use fluorescent conjugates.

48 


3 


3.1 PromoFluor Dyes. ................... 49 

3.2 PromoFluor Labeling Kits. ............. 52 

3.2.1 PromoFluor Biomolecules Labeling Kits ... 52 

3.2.2 PromoFluor DNA Labeling Kits ......... 53 

3.3 PromoFluor Labeled Biomolecules ....... 54 

3.3.1 PromoFluor Labeled Antibodies ......... 54 

3.3.2 PromoFluor Labeled Phalloidin ......... 54 

3.3.3 PromoFluor Labeled Nucleotides ........ 55 

3.3.4 PromoFluor Labeled Biotin 

and Streptavidin .................... 56 

3.4 Phycobiliprotein Dyes ................ 57 

3.5 Phycobiliprotein Labeled Biomolecules. ... 59 

3.6 PromoFluor Antifade Reagent .......... 60

3 Fluorescent Labeling 49 

3.1 PromoFluor Dyes 

Applications 

qualified for a wide range of cell and 

PromoFluor Amino-Modified Labels 

molecular biology applications. In ad- 

Labeling biomolecules such as 

dition to the standard dyes, specifically 

PromoFluor Amino-Modified Labels 

proteins, antibodies, and nucleic acids 

designed PromoFluor-LSS dyes offer an 

are intended for the covalent labeling 


extra large Stokes shift, which makes 

of biomolecules containing a carboxy 


them ideally suited for multicolor label- 

group, e.g., glycoproteins. The amino 

Fluorescent in situ hybridization 

ing and flow cytometry applications. 

group of PromoFluor Amino-Modified 

(FISH) 

Labels can be cross-coupled to carboxy 

Fluorescence correlation spectroscopy 

All PromoFluor dyes are provided 

groups through a mild in situ activation. 

(FCS) 

DNA and protein microarrays 

either as free carboxylic acids or in three 

reactive variants ready for covalent cou- 

PromoFluor Maleimides 

pling to biomolecules: amine-reactive 

Features 

PromoFluor NHS-Esters, PromoFluor Ami- 

PromoFluor Maleimides are intended for 

no-Modified Labels, and thiol-reactive 

covalently labeling biomolecules con- 

Strong light absorption and high 

PromoFluor Maleimides. 

taining a thiol group (e.g. proteins). A 

fluorescence intensity 

Very photostable 

PromoFluor NHS-Esters 

neutral thiol-reactive label has little influence 

on the isoelectric point of a protein. 

Highly fluorescenct over a broad pH 

In effect, PromoFluor Maleimides have 

range (pH 4 - 9) 

Water-soluble without the need to 

use organic solvents 


PromoFluor NHS-Esters (N-hydroxysuccinimidyl-esters) 

are intended for covalently 

labeling biomolecules containing a 

primary amino group (all peptides/pro- 

an advantage over amino group labels 

because they do not interfere with techniques 

like differential 2D gel electrophoresis. 

Since most proteins contain 

3 Fluorescent 

Labeling 


teins contain at least one amino group 

only a few thiol functional groups, thiol- 


at the N-terminus). Moreover, amino 

reactive dyes can be used to selectively 

groups can also be incorporated in other 

modify a protein or peptide containing 

PromoFluor dyes are powerful fluoro- 

biomolecules without changing their 

a thiol group at a specific site. Moreover, 

phores spanning almost the whole visible 

activity or functionality. The most com- 

these dyes are well suited for labeling 

and infrared light spectrum (see Table 1). 

mon examples are 5’-amino-modified 

oligonucleotides. Maleimides couple to 

They are superior to many other com- 

DNA oligomers and cDNA containing 

thiol groups under very mild conditions 

monly used fluorescent dyes and well 

aminoallyl-dU. 

at a pH around 7. 

Fig. 1: Left: Cell structures of HeLa cells visualized using primary anti-beta-tubulin antibodies and secondary PromoFluor 488-labeled 

antibodies (green) as well as primary anti-centromere antibodies and secondary PromoFlour 547-labeled antibodies (red). 

Middle: Rat cytokeratin (green) detected with primary rabbit anti-cytokeratin antibodies and secondary anti-rabbit IgGs labeled 

with PromoFluor 488. Actin (red) visualized using mouse anti-actin antibodies and secondary goat anti-mouse IgGs labeled with 

PromoFluor 520LSS. Right: Solid-state fluorescence of PromoFluor 633.

50 

3 Fluorescent Labeling 

Name 

Absorption 

Maximum 

[nm] 

Emission 

Maximum 

[nm] 

Description 

Laser Excitation Source* 

PromoFluor 350 354 431 Alternative to Alexa Fluor ® 350, 

AMCA, AMCA-X 

PromoFluor 405 400 420 Alternative to Alexa Fluor ® 405, Pacific Blue, 

Cascade Blue 

PromoFluor 415 418 467 Alternative to Pacific Blue, Spectrum Aqua, 

and Diethylaminocoumarin 

PromoFluor 488LSS 485 560 Dye with extra large Stokes shift. 

Ideally suited for multicolor labeling 

and FACS applications 

PromoFluor 488 493 521 Alternative to Fluorescein (FITC), Alexa 

Fluor ® 488 and Cy TM 2 

Argon laser or 

UV-light 

Blue diode laser 

Blue diode laser 

Argon 488 laser 


3 Fluorescent 

Labeling 

PromoFluor 488 

Premium 

490 516 Very photostable alternative to Fluorescein 

(FITC), Alexa Fluor ® 488, Cy TM 2 






PromoFluor 505 505 530 Alternative to Rhodamine Green TM Argon 488 laser 











Tetramethylrhodamine (TMR and TRITC) 


Tetramethylrhodamine (TMR and TRITC) 

PromoFluor 555 557 574 Alternative to Alexa Fluor ® 555, Cy TM 3 

and Tetramethylrhodamine (TMR) 




Nd:YAG laser 

Helium-Neon 543 laser 

Helium-Neon 543 laser 

PromoFluor 590 581 598 Alternative to Texas Red ® Helium-Neon 594 laser 

PromoFluor 610 610 630 Alternative to Texas Red ® Helium-Neon 594 laser 

PromoFluor 633 637 657 Alternative to Alexa Fluor ® 633 Helium-Neon 633 laser 

and 635 diode laser 


Cy TM 5, and Allophycocyanin 

Krypton-Argon 647 

laser 

PromoFluor 670 673 694 Alternative to Alexa Fluor ® 680 and Cy TM 5.5 Helium-Neon laser 

PromoFluor 680 690 709 Alternative to Alexa Fluor ® 680, Cy TM 5.5 Helium-Neon laser 

PromoFluor 700 704 723 Alternative to Alexa Fluor ® 700 Far-red diode lasers 

PromoFluor 750 747 776 Alternative to Alexa Fluor ® 750, Cy TM 7 Far-red diode lasers 

PromoFluor 770 771 801 Alternative to Alexa Fluor ® 750 and Cy TM 7 Xenon-arc lamp or 

dye-pumped laser 

PromoFluor 780 783 800 Near infrared dye allowing for deep tissue 

penetration and low background 

PromoFluor 840 844 - Near infrared dye allowing for deep tissue 

penetration and low background 

Xenon-arc lamp or 


Xenon-arc lamp or 


Table 1: List of currently available PromoFluor dyes (for new PromoFluor dyes that may be added in the future please visit our website). 

*PromoFluor dyes are compatible with common excitation sources including diode lasers, LEDs, tungsten lamps, xenon arc lamps, and 

custom made systems.


Excitation / Emission [%] 

100 

80 

60 

40 

Fig. 2: Excitation (left) and 

emission (right) spectra of 

PromoFluor 488LSS. 

20 

300 350 400 450 500 550 600 650 700 

Wavelength [nm] 

3 Fluorescent 

Labeling 

PromoFluor ChipPacks 

PromoFluor ChipPacks are pre-packed 

PromoFluor dyes intended to label cDNA 

for microarray assays. The packs con- 

tain 20 µg aliquots of PromoFluor 555 

and PromoFluor 647 NHS-Ester. These 

aliquots are sufficient for labeling the 

amount of cDNA synthesized in a standard 

reverse transcription assay with 

enzymatic incorporation of aminoallyldUTP. 

Customized PromoFluor Chip- 

Packs with other PromoFlour dyes are 

available on request. 

Product Size Catalog Number* 

PromoFluor Carboxylic Acid 

PromoFluor NHS-Ester 

PromoFluor Amino-Modified Label 

PromoFluor Maleimide 

PromoFluor ChipPack 


1 mg 

5 mg 

1 mg 

5 mg 

1 mg 

5 mg 

0.5 mg 

1 mg 

5 mg 

20 labeling reactions 



PK-PFXXX-0-01 

PK-PFXXX-0-05 

PK-PFXXX-1-01 

PK-PFXXX-1-05 

PK-PFXXX-2-01 

PK-PFXXX-2-05 

PF-PFXXX-3-005 

PK-PFXXX-3-01 

PK-PFXXX-3-05 

PK-PF-CP-S 

PK-PF-CP-M 

PK-PF-CP-L 

Coupling protocols for proteins are available on our website: www.promokine.info 

* XXX- stands fo the respective PromoFluor dye (e.g. PF-350, PF-405, PF-415 etc., see Table 1; please see our website for an up-to-date list of available PromoFluor Dyes). 

Please note: Different standard sizes are available for PF-488 and PF-505.

52 


3.2 PromoFluor Labeling Kits 

3.2.1 PromoFluor Biomolecules Labeling Kits 

Applications 

Labeling biomolecules such as 





(FISH) 


(FCS) 

Features 

Convenient ready-to-use kits 

Contain superior PromoFluor dyes 

(see chapter 3.1) 

Highly efficient labeling 

Very bright and stable fluorescent 

signals 

PromoFluor Biomolecules Labeling Kits 

have been developed for the convenient 

and highly efficient labeling of 

target proteins or antibodies with 

PromoKine’s superior PromoFluor 

dyes. They contain the respective 

PromoFluor NHS-Ester as well as all the 

other components required for labeling 

biomolecules. 

PromoFluor Labeling Kits work with all 

molecules with a primary amino group 

such as peptides, proteins, 5’-aminomodified 

DNA oligomers, and cDNA 

containing aminoallyl-dU. 

3 Fluorescent 

Labeling 

Fig. 3: Cell structures labeled with 

antibodies bound to green-fluorescing 

PromoFluor 505 (left) and red-fluorescing 

PromoFluor 647 dyes (red). 

Illustrations kindly provided by Covalys 

Biosciences AG, Switzerland. 


PF-XXX-Labeling-Kit 


5 reactions 

10 reactions 

PK-PF-LK-XXX-05 

PK-PF-LK-XXX-10 

XXX- stands fo the respective PromoFluor dye (PF-405, PF-415, PF-488, PF-488LSS, PF-514LSS, PF-546, PF-555, PF-633, PF-647, PF-670, PF-750, PF-770; 

please see our website for an up-to-date list of available PromoFluor Labeling Kits).


3.2.2 PromoFluor DNA Labeling Kits 

Applications 

of the dye-dUTP analogs combined with 

the outstanding stability and quantum 

DNA labeling by PCR 

yield of the fluorophores make the kits 

DNA labeling by nick translation 

the ideal choice for all typical DNA la- 

Labeling of probes for FISH, micro- 

beling applications such as FISH, single 

arrays, Southern hybridizations, etc. 

molecule detection, microarray gene 

expression profiling, and other nucleic 

Features 

acid hybridization assays. PromoKine’s 

PCR Labeling Kits contain all reagents 

Convenient and fast DNA labeling 

required for highly efficient, rapid, and 

Optimal incorporation of labeled 

easy-to-perform DNA labeling (exclud- 

dUTPs 

ing primers and template DNA). The 

Highly fluorescent and stable fluoro- 

Nick Translation Labeling Kits contain all 

phores 

reagents required for efficient, rapid and 

easy-to-perform DNA labeling by nick 

PromoKine’s PCR Labeling Kits and 

translation (excluding template DNA). 

Nick Translation Labeling Kits have been 

The labeled dUTPs are also available 

designed for convenient direct DNA 

separately (see chapter 3.3.4). 

labeling by PCR or nick translation using 

Taq polymerase or Polymerase I/ 

DNase I. The fluorescently labeled dUTP 

analogs provided with the kits have been 

In addition, we also provide a kit for nonfluorescent 

labeling of DNA with biotin. 

The biotinylated DNA can then interact 

Fig 4: PromoKine’s DNA Labeling Kits 

have been used to generate fluorescently 

labeled DNA probes for staining specific 

3 Fluorescent 

Labeling 

optimized for enzymatic incorporation 

with streptavidin or strepatavidin conju- 

chromosome regions. 

into DNA by using a proprietary linker 

gates (see also chapter 3.3.3 and 3.6) for 

technology. The high incorporation rates 

detection or purification applications. 


PromoFluor-430 PCR Labeling Kit 

Blue-green fluorescent DNA labeling 

by PCR 

10 reactions 

50 reactions 

PK-PF430-PCRLK-10 



Green-fluorescent DNA labeling 

by PCR 

10 reactions 

50 reactions 




Yellow-fluorescent DNA labeling 

by PCR 

10 reactions 

50 reactions 




Orange-fluorescent DNA labeling 

by PCR 

10 reactions 

50 reactions 




TexasRed PCR Labeling Kit 

Red-fluorescent DNA labeling 

by PCR 


by PCR 

10 reactions 

50 reactions 

10 reactions 

50 reactions 

Biotin PCR Labeling Kit Kit for biotin-labeling of DNA by PCR 100 reactions 

500 reactions 

PromoFluor-430 Nick Translation 

Labeling Kit 


Labeling Kit 


Labeling Kit 


Labeling Kit 


Labeling Kit 

Blue-green fluorescent DNA labeling 

by nick translation 

Green-fluorescent DNA labeling 


Yellow-fluorescent DNA labeling 




Red-fluorescent DNA labeling 


10 reactions 

50 reactions 

10 reactions 

50 reactions 

10 reactions 

50 reactions 

10 reactions 

50 reactions 

10 reactions 

50 reactions 



PK-PFTXR-PCRLK-10 

PK-PFTXR-PCRLK-50 

PK-CA-BIO-LK100 

PK-CA-BIO-LK500 

PK-PF430-NTLK-10 











Please see our website for an up to date list of available PromoFluor DNA Labeling Kits.

54 


3.3 PromoFluor Labeled Biomolecules 

3.3.1 PromoFluor Labeled Antibodies 

Applications 




(FISH) 


(FCS) 

Fig 5: Tubulin and centromere structures 

of HeLa cells visualized by using 

specific primary antibodies and 

secondary antibodies labeled with 

PromoFluor 488 and PromoFluor 555. 

Features 

High quality and ready-to-use 

Labeled with PromoFluor dyes 

High intensity yields and stable 

fluorescent signals 

Product Size* Catalog Number 

3 Fluorescent 

Labeling 

Ready-to-use antibody conjugates of all 

PromoFluor dyes (see chapter 3.1.) are 

available as goat anti-human IgGs, goat 

anti-rabbit IgGs, and goat anti-mouse 

IgGs. The labeled secondary antibodies 

(polyclonal) are affinity-purified and 

dissolved in 100 mM PBS (pH 7.5) at a 

concentration of 1 mg/ml. Each antibody 

comes with a batch-specific absorption 

spectrum. 

PF XXX-Goat Anti-Mouse IgG 1 mg PK-PFXXX-AK-M1 

PF-XXX-Goat Anti-Rabbit IgG 1 mg PK-PFXXX-AK-R1 

PF-XXX-Goat Anti-Human IgG 1 mg PK-PFXXX-AK-H1 


* Other sizes (e.g. bulk sizes) available on request. 

XXX stands for the respective PromoFluor dye (PF405, PF488, PF488LSS, PF500LSS, PF505 etc). 

Please see chapter 3.1 for details. 

3.3.2 PromoFluor Labeled Phalloidin 

Applications 

Localization and visualization of actin 

filaments 


as well as for visualizing individual actin 

filaments in vitro. Ready-to-use phalloidin 

conjugates of all PromoFluor dyes 

are available (10 nmol, 300 units). 

Features 


Labeled with the superior PromoFluor 

dyes (see chapter 3.1) 

Yields highly intensive and stable 


Fig. 6: Phalloidin and centromere 

structures of HeLa cells stained using a 

PromoFluor 647-Phalloidin conjugate 

and primary anti-centromer antibodies 

in combination with secondary antibodies 

labeled with PromoFluor 488. 

Phalloidin binds very selectively to actin 

(much more tightly to actin filaments 

than to actin monomers). In combination 

with conjugated PromoFluor dyes, 

phalloidin is enormously useful in localizing 

actin filaments in living or fixed cells 

Product Size* Catalog Number** 

PF-XXX-Phalloidin 10 nmol PK-PFXXX-7-01 


* 10 nmol are sufficient for staining approximately 300 microscopic slides. 

** XXX stands for the respective PromoFluor dye (PF-405, PF-488, PF-488LSS, PF-500LSS, PF-505 etc). 

Please see chapter 3.1 for details.


3.3.3 PromoFluor Labeled Nucleotides 

Applications 

Fluorescent labeling of nucleic acids 


(FISH) 

Multicolor FISH (chromosome 

painting) 

Microarrays 

Features 


Labeled with superior PromoFluor 




Minimal steric hindrance 

PromoKine provides aminoallyl-dUTP 

(deoxyuridine-triphosphate) conjugates 

for all PromoFluor dyes (see 3.1). The 

labeled nucleotides offer excellent properties 

with regard to signal intensity, 

water solubility, and stability. In addition, 

the low molecular weight of the dyes 

results in minimal steric hindrance and 

unproblematic incorporation into DNA 

using conventional enzymatic techniques 

like PCR/RT-PCR. Aminoallyl-dUTP can 

be incorporated into DNA by Taq polymerase, 

Klenow fragment, and reverse 

transcriptases. All PromoFluor labeled 

nucleotides are delivered as 1 mM readyto-use 

aqueous solutions. 

Fig 7: 2-color labeling of chromosomes 

1 and 8 using dUTPs conjugated to 

PromoFluor 505 and PromoFluor 560. 

Picture kindly provided by Applied 

Spectral Imaging. 

Fig 8: Fluorescence laser scan of a microarray slide using PromoFluor dyes. Different 

matching oligonucleotides, negative controls, and reference oligonucleotides were 

spotted in 25 replicates and at 4 different concentrations to epoxysilane coated slides 

and hybridized with PromoFluor labeled samples. Picture kindly provided by the 

Functional Genomics Center Zurich, Switzerland. 

3 Fluorescent 

Labeling 


PF-XXX-aadUTP 100 µl PK-PFXXX-8-100 

PromoFluor-430-dUTP (NT) 10 µl 

50 µl 

PromoFluor-430-dUTP (PCR) 10 µl 

50 µl 


50 µl 


50 µl 


50 µl 


50 µl 


50 µl 


50 µl 


50 µl 


50 µl 

TexasRed-dUTP (PCR) 10 µl 

50 µl 

Biotin-dATP (PCR) 

10 µl (1 mM) 

50 µl (1 mM) 

Biotin-dCTP (PCR) 

200 µl (1 mM) 

1 ml (1 mM) 

Biotin-dUTP (PCR) 

200 µl (1 mM) 

1 ml (1 mM) 

PK-PF430-DUTP-10N 


PK-PF430-DUTP-10P 


















PK-PFTXR-DUTP-10P 

PK-PFTXR-DUTP-50P 

PK-CA-BIO-DATP-10P 

PK-CA-BIO-DATP-50P 

PK-CA-BIO-DCTP-200P 

PK-CA-BIO-DCTP-1000P 

PK-CA-BIO-DUTP-200P 

PK-CA-BIO-DUTP-1000P 


* XXX stands for the respective PromoFluor dye (PF-405, PF-488, PF-488LSS, PF-500LSS, PF-505 etc). Please see chapter 3.1 for details.

56 


3.3.4 PromoFluor Labeled Biotin and Streptavidin 

Applications 

the highest known affinity between any 

high-quality streptavidin and biotin con- 

protein and its ligand. Extensive chemical 

jugates with R-Phycoerythrin (R-PE), B- 


modifications have little effect on this af- 

Phycoerythrin (B-PE), Allophycocyanin 


finity. Streptavidin is a tetrameric 53 kDa 

(APC), cross-linked Allophycocyanin (cl- 


protein. Since it lacks any carbohydrate 

APC), and FITC (see chapter 3.6). 

(FISH) 

modification and has a near-neutral pH, 


it has the advantage of much lower non- 

(FCS) 

specific binding than avidin. On the other 

hand, streptavidin is less watersoluble 

Features 

than avidin. 

Ready-to-use streptavidin and biotin 


conjugates of all PromoFluor dyes (see 

Labeled with superior PromoFluor 

chapter 3.1.) are available. The strepta- 


vidin conjugates are affinity-purified and 


dissolved in 100 mM PBS (pH 7.5) at a 


concentration of 1 mg/ml. The biotin 

Fig. 9: Staining of plasma membrane- 

conjugates are affinity-purified and lyo- 

bound neurokinin-1 receptor (red) in a 

Streptavidin has a very strong affinity 

philized. Each conjugate comes with a 

U2OS cell using a PromoFluor 547-con- 

3 Fluorescent 

Labeling 

for biotin with a dissociation constant 

of approximately 10-15 mol/l. This is 

batch-specific absorption spectrum. 

In addition, PromoKine also provides 

jugate. Picture kindly provided by Covalys 

Biosciences AG, Switzerland. 


PF-XXX-Streptavidin Conjugate 1 mg in 1 ml PBS PK-PFXXX-5-01 

PF-XXX-Biotin Conjugate 1 mg PK-PFXXX-6-01 


The range of PromoKine fluorescent labeled biomolecules is being continuously extended. Please visit regularly our 

website www.promokine.info 

* XXX stands for the respective PromoFluor dye (PF-405, PF-488, PF-488LSS, PF-500LSS, PF-505 etc). 

Please see chapter 3.1 for details.


3.4 Phycobiliprotein Dyes 

Applications 

sitive cell and molecular biological ap- 

545 nm and emitting strongly at 572 nm 

plications such as immunofluorescence 

(see Fig. 11). Since it is less “sticky“ than 

Labeling of biomolecules such as 

microscopy, flow cytometry, microarrays, 

R-Phycoerythrin and demonstrates less 


enzyme kinetic studies, etc. They show 

non-specific binding, B-phycoerythrin 


optimal quantum yields (up to 98%) and 

is better suited for applications that are 


extinction coefficients as well as large 

sensitive to background signals. 


(FISH) 

Stokes shifts. The fluorescence yield is 

equivalent to at least 30 unquenched 

Allophycocyanin 


fluorescein or 100 rhodamine molecules 

(FCS) 

at comparable wavelengths (on a molar 

Allophycocyanin (APC), another member 

FRET assays 

basis), and fluorescence is not quenched 

of the light-harvesting phycobiliprotein 

Features 

by external agents since it is protected 

by covalent binding to the protein back- 

family, absorbs red light at 650 nm and 

has a maximal emission at 660 nm (see 

bone. PromoKine’s phycobiliproteins 

Fig. 12). Allophycocyanin and its conju- 

Strong light absorption and high 

can easily be linked to antibodies. The 

gates are both brighter and more photo- 

fluorescence yields 

sensitivity of antibodies conjugated with 

stable than competing, commonly used 

Excellent stability and high 

R-PE and B-PE is usually five to ten times 

products. 

fluorescence over a broad pH range 

greater than that of the corresponding 

Fluorescent signals are not quenched 

by external agents 



fluorescein conjugate. 

R-Phycoerythrin 

SMCC*-activated R-Phycoerythrin and 

SMCC*-activated Allophycocyanin are 

chemically activated (maleimide group 

from SMCC) for easy reactions with 

3 Fluorescent 

Labeling 


R-Phycoerythrin (R-PE) has a strong 

sulfohydryl (SH) groups such as reduced 

absorption peak at 565 nm and a sec- 

cysteine residues from proteins and 

Phycobiliproteins are water-soluble 

ondary absorption peak at 492 nm. The 

antibodies. The R-PE and APC labeled 

proteins with covalently bound chro- 

emission peak is at 575 nm (see Fig. 

antibodies are highly fluorescent and 

mophores present in cyanobacteria 

10). This protein is commonly used for 

ideally suited for applications such as 

and certain algae (rhodophytes, cryp- 

fluorescent immunolabeling, particularly 

immunofluorescence microscopy or flow 

tomonads, glaucocystophytes) that 

for applications involving fluorescence- 

cytometry. 

capture light energy during photosynthesis. 

The major phycobiliproteins are 

R-Phycoerythrin, B-Phycoerythrin, and 

Allophycocyanin. 

Phycobiliproteins can be used as fluorescent 

dyes to label biomolecules to obtain 

activated cell sorting (FACS). 

B-Phycoerythrin 

B-Phycoerythrin (B-PE) has slightly different 

spectral characteristics than R-Phy- 

* Succinimidyl-4-(N-Maleimidomethyl)Cyclohexane- 

1-Carboxylate (SMCC) is a non-cleavable, membranepermeable 

crosslinker containing an amine-reactive N- 

hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive 

maleimide group. NHS esters react with primary amines 

at pH 7-9 to form stable amide bonds, while maleimides 

react with sulfhydryl groups at pH 6.5-7.5 to form stable 

thioether bonds. 

specific probes or markers for highly sen- 

coerythrin absorbing strongly at about 


100 

80 

60 

40 



20 

R-Phycoerythrin. 

320 360 400 440 480 520 560 600 

640 680 

Wavelength [nm]

58 



100 

80 

60 

40 



B-Phycoerythrin. 

20 

320 360 400 440 480 520 560 600 


640 680 


3 Fluorescent 

Labeling 

100 

80 

60 

40 



Allophycocyanin. 

20 

320 360 400 440 480 520 560 600 


640 680 


R-Phycoerythrin, lyophilized 5 mg PK-PF-RPE-05 

SMCC-activated R-Phycoerythrin, 

lyophilized 

5 mg PK-PF-IRPE-05 

B-Phycoerythrin, lyophilized 5 mg PK-PF-BPE-05 

Allophycocyanin, lyophilized 5 mg PK-PF-APC-05 

Allophycocyanin (cross-linked), lyophilized 5 mg PK-PF-APC-05CL 

SMCC-activated Allophycocyanin, 

lyophilized 

5 mg PK-PF-IAPC-05 


We also provide high quality streptavidin and biotin conjugates with R-Phycoerythrin, B-Phycoerythrin, Allophycocyanin, and 

cross-linked Allophycocyanin. Please see chapter 3.5 for more information.


3.5 Phycobiliprotein Labeled Biomolecules 

Applications 




(FISH) 

Fluorescence immunoassay (FIA) 

Features 

High quality 

Ready-to-use 



Avidin and streptavidin have a very 

strong affinity for biotin with a dissociation 

constant of approximately 10-15 

mol/l. This is the highest known affinity 

between any protein and its ligand. Extensive 

chemical modifications have little 

effect on this affinity. 

Avidin is a tetrameric glycoprotein with 

a combined mass of approximately 67 

kDa. Due to its carbohydrate content 

and basic pI, avidin shows relatively high 

nonspecific binding. 

Streptavidin is a tetrameric 53 kDa protein. 

Since it lacks any carbohydrate 

modification and has a near-neutral 

pH, it has the advantage of much lower 

nonspecific binding than avidin. On the 

other hand, streptavidin is less water 

soluble than avidin. 

Ready-to-use avidin, streptavidin, and 

biotin conjugates of R-Phycoerythrin, 

B-Phycoerythrin, and Allophycocyanin 

are available. These ready-to-use conjugates 

are very useful second-step reagents 

for staining with biotinylated or 

streptavidin-coupled antibodies and can 

be used in (strept)avidin/biotin-labeling 

systems for flow cytometry, fluorescent 

immunoassays, immunohistochemistry, 

and immunofluorescence microscopy. 


Streptavidin-R-Phycoerythrin 

Streptavidin-B-Phycoerythrin 

Streptavidin-Allophycocyanin 

Streptavidin-Allophycocyanin 

(cross-linked) 

Biotin-R-Phycoerythrin 

Biotin-B-Phycoerythrin 

Biotin-Allophycocyanin 

1 mg in 1 ml Buffer 














PK-PF-SA-RPE-01 

PK-PF-SA-RPE-05 

PK-PF-SA-BPE-01 

PK-PF-SA-BPE-05 

PK-PF-SA-APC-01 

PK-PF-SA-APC-05 

PK-PF-SA-APC-01CL 

PK-PF-SA-APC-05CL 

PK-PF-BT-RPE-01 

PK-PF-BT-RPE-05 

PK-PF-BT-BPE-01 

PK-PF-BT-BPE-05 

PK-PF-BT-APC-01 

PK-PF-BT-APC-05 

Biotin-Allophycocyanin (cross-linked) 1 mg in 1 ml Buffer PK-PF-BT-APC-01CL 

Streptavidin-Fluorescein-5-isothiocyanate 

(FITC) 



PK-PF-SA-FITC-01 

PK-PF-SA-FITC-05 

3 Fluorescent 

Labeling 


60 


3.6 PromoFluor Antifade Reagent 

Applications 

Mounting medium for fluorescence 

microscopy 

Features 

Significantly increased photostability 

Compatible with many common dyes 

Conserves cell preparations 

Convenient packaging 

Irreversible photobleaching processes 

lead to a loss of fluorescent signal sensitivity 

particularly when high intensity 

excitation light is used for a longer duration, 

and the target molecules in samples 

are in low abundance. PromoFluor Antifade 

Reagent significantly increases 

the photostability of fluorochromes in 

fixed cells, fixed tissues, and cell-free 

preparations. It is compatible with many 

common dyes and dye complexes resulting 

in prolonged signals and improved 

image contrast especially in multicolor 

applications. Moreover, it gently solidifies 

after the attachment of the cover 

slide, permanently conserving the cell 

preparation and fixing the coverslip. 

PromoFluor Anitfade Reagent makes 

slide immobilization using e.g. nail polish 

unnecessary. Samples can be saved for 

months after mounting, retaining fluorescence 

during prolonged storage. 

PromoFluor Antifade Reagent comes 

premixed in a convenient dropper bottle. 

3 Fluorescent 

Labeling 

Fig. 13: Immunofluorescence imaging 

of a human keratinocyte stained with 

FITC-labeled antibodies without (top) 

and with (bottom) PromoFluor Antifade 

Reagent at different excitation intervals 

(5, 10, 15, 20, 25 seconds). 


PromoFluor Antifade Reagent 

Mounting Medium for Fluorescence 

Microscopy 

8 ml PK-PF-AFR1 


Gene Cloning, Expression, 

4 

and Silencing 

Gene cloning is the process of making multiple copies of a defined 

DNA sequence. Since its establishment it has become a core technique 

in molecular biology, and it enables a wide array of biological 

experiments and practical applications. Amplifying DNA has also 

allowed the study of gene expression, including the examination of 

genetic information transcribed and translated into RNA and proteins. 

In contrast, gene silencing has also grown into an important 

technique for analyzing gene functions.

62 


4 

Gene Cloning, Expression, 

and Silencing 

4.1 Plasmid Vectors ..................... 63 

4.1.1 pPK-CMV Expression Vectors .......... 63 

4.1.2 pPK-CMV Reporter Vectors. ........... 64 

4.1.3 pPK-CMV Fusion Vectors ............. 65 

4.2 StarGate Cloning Systems ............. 66 

4.2.1 StarGate Combinatorial Cloning System .. 66 

4.2.2 StarGate Mutagenesis System ......... 67 

4.2.3 StarGate Fusion Cloning System ........ 68 

4.3 Gene Silencing ..................... 70 

4.3.1 GeneOFF shRNA Vector Kits .......... 70 

4.3.2 GeneOFF siRNA Generation Kit ........ 71

4 Gene Cloning, Expression, and Silencing 63 

4.1 Plasmid Vectors 

4.1.1 pPK-CMV Expression Vectors 

Applications 

Constitutive high-level 

transgene expression 

Expression of native and 

HA-tagged proteins 

Features 

Optimized CMV promoter provides 

high level constitutive expression 

Suited for a wide variety of cell types 

for both stable and transient 

gene expression 

Small vector size ensures high 

transfection efficiency 

Multiple cloning site with most 

commonly-used restriction sites 

The pPK-CMV Expression Vectors contain 

a modified human cytomegalovirus 

(CMV) based promoter/enhancer sequence 

that has been optimized for high 

gene expression. Sequence motives that 

limit expression have been removed, 

while others that promote high expression 

levels have been retained. Consequently, 

these vectors yield significantly 

higher expression levels than other expression 

vectors such as pcDNA3.1 that 

are based on the standard CMV promoter. 

In addition, the plasmid backbones 

are optimized to allow for very small vector 

sizes and higher plasmid yields when 

amplified in E. coli. 

Three versions of the pPK-CMV Expression 

Vector are available: 

pPK-CMV-E1 does not have an expression 

tag. It is intended for expressing 

recombinant proteins in the native 

forms. pPK-CMV-E2 comes with an N- 

terminal HA (hemagglutinin) fusion tag. 

pPK-CMV-E3 comes with a C-terminal 

HA-fusion tag. The HA fusion tags allow 

easy detection and purification of proteins. 

All three Expression Vectors come 

with a positive control vector containing 

a CAT gene. 

pPK-CMV-E1 

pPK-CMV-E2 

pPK-CMV-E3 

pUC ori 

ATG 

Bgl II 

Xho I 

Hind III 

EcoR I 

Kpn I 

Apa I 

Sma I 

BamH I 

Not I 

Xba I 

HA 

Bgl II 

Xho I 

Hind III 

EcoR I 

Kpn I 

Apa I 

Sma I 

BamH I 

CMV 

Bgl II 

Xho I 

Hind III 

EcoR I 

Kpn I 

Apa I 

Sma I 

BamH I 

Not I 

Xba I 

pPK-CMV Expression Vector 

4.2 kb 

HSV pA 

SV40pA 

Kan/Neo 

SV40p 

Fig. 1: Three versions of the pPK-CMV 

Expression Vectors are available for 

convenient and efficient gene cloning 

and expression. 

HA 

Term 

AmpP 

4 Gene Cloning, Expression, 

and Silencing 


pPK-CMV-E1 Expression Vector Kit 25 µg PK-MB-P003100 




64 

4 Gene Cloning, Expression, and Silencing 

4.1.2 pPK-CMV Reporter Vectors 


and Silencing 

Applications 

Transient, high-level reporter gene/ 

target gene expression in mammalian 

cells and tissues 

Monitoring transfection efficiency 

and gene expression 

Features 

High expression of five popular 

reporter genes 

Suited for in vitro and in vivo 

gene expression 

Convenient multiple cloning site 

for optimized cloning and expression 

of target genes 


Small vector size ensures high trans- 

fection efficiency and high plasmid 

yield in E. coli 

The pPK-CMV Reporter Vectors have 

been designed for high level, transient 

expression of reporter or target genes. 

They contain an optimized CMV promoter 

followed by intron A from the 

CMV immediate-early gene (IE). This 

ensures extremely high transient expression 

in a wide variety of mammalian cells 

and tissues. In addition, the vectors contain 

an optimized plasmid backbone that 

has been extensively altered to achieve 

optimal plasmid production in E. coli. 

The vectors are available without a reporter 

gene to clone and express a gene 

of interest (blank vector) as well as with 

the following reporter genes: 

β-galactosidase (lacZ), green fluorescent 

protein (GFP), luciferase (Luc), chloramphenicol 

acetyltransferase (CAT), and 

secreted alkaline phosphatase (SEAP). 

pPK-CMV Reporter Vector 

4.2 kb 

Fig. 2: The pPK-CMV Reporter Vector 

allows strong transient expression of 

target genes as well as different reporter 

genes in many cell types and tissues. 


Reporter 

MCS 

pUC ori 

Acc I 

Sal I 

Eco72 I 

Bcl I 

EcoR V 

Not I 

Bbe I 

Ehe I 

Kas I 

Nar I 

BamH I 

CMV 

β-Gal 

GFP 

Luciferase 

CAT 

SEAP 

pPK-CMV-R1 Reporter Vector (β-Gal) 25 µg PK-MB-P010200 

pPK-CMV-R2 Reporter Vector (CAT) 25 µg PK-MB-P020200 

pPK-CMV-R3 Reporter Vector (Luc) 25 µg PK-MB-P030200 

pPK-CMV-R4 Reporter Vector (GFP) 25 µg PK-MB-P040200 

pPK-CMV-R5 Reporter Vector (SEAP) 25 µg PK-MB-P050200 

pPK-CMV-R0 Reporter Vector (Blank) 25 µg PK-MB-P000200 

HSV pA 

SV40pA 

Kan 

AmpP 

SV40p 


Please see chapter 1 for luciferase, beta-Gal, and GFP reporter assays.


4.1.3 pPK-CMV Fusion Vectors 

Applications 

efficient selection in both E. coli (using 

pPK-CMV-F4 (N-Luc) has a Luciferace 

kanamycin) and mammalian cells (using 

coding sequence without a stop codon 

Constitutive high-level GFP or 

neomycin). 

inframe in the MCS. The gene of inter- 

Luciferase fusion protein expression 

An SV40 polyadenylation signal provides 

est can therefore be expressed with Lu- 

Creating GFP or Luciferase 

efficient transcription termination and 

ciferase at the N-terminus. 

expressing stable cell lines 

polyadenylation - thus ensuring stable 

mRNA. A pUC origin of replication al- 

Features 

Optimized CMV promoter provides 

high level constitutive expression 


lows for high copy numbers in E. coli. 

The vectors can be used for either transient 

transfection or for generating stable 

cell lines expressing only the reporter 

gene or the fusion target-reporter gene 

pPK-CMV-F1 

(C-GFP, 4965 bp) 

pPK-CMV-F2 

(N-GFP, 4962 bp) 

Pst I (2) 

Bgl II 

Xho I 

Hind III 

EcoR I (2) 

Pst I (2) 

Bgl II 

Xho I 

Hind III 

EcoR I (2) 

GFP 

GFP 

EcoR I (2) 

Pst I (2) 

Kpn I 

Apa I 

Sma I 

Xma I 

BamH I 

Not I 

Xba I 

Stop 

EcoR I (2) 

Pst I (2) 

Kpn I 

Apa I 

Sma I 

Xma I 

BamH I 

Not I 

Xba I 

Small vector size ensures high 

construct. 

transfec tion efficiency 

Two multiple cloning sites upstream or 

downstream of the reporter gene 

The pPK-CMV Fusion Vectors have been 

Four versions of the vector are available 

for convenient and efficient gene cloning 

and expression of the target gene fused 

with a reporter gene: 

pPK-CMV-F1 (C-GFP) has a GFP cod- 

pPK-CMV-F3 

(C-Luc, 5898 bp) 

pPK-CMV-F4 

(N-Luc, 5895 bp) 

Pst I (2) 

Bgl II 

Xho I 

Hind III 

EcoR I (2) 

Pst I (2) 

Bgl II 

Xho I 

Hind III 

EcoR I (2) 

LUC 

LUC 

EcoR I (2) 

Pst I (2) 

Kpn I 

Apa I 

Sma I 

Xma I 

BamH I 

Not I 

Xba I 

Stop 

EcoR I (2) 

Pst I (2) 

Kpn I 

Apa I 

Sma I 

Xma I 

BamH I 

Not I 

Xba I 

designed for high-level expression of 

ing sequence with a stop codon inframe 

GFP or Luciferase fusion proteins and 

for creating GFP or Luciferase express- 

in the MCS. The gene of interest can 

therefore be expressed with GFP at the 

+ Intron 

pT7 

SV40pA 

ing stable cell lines. They contain an 

C-terminus. 

optimized CMV promoter and an intron 

sequence, which ensures extremely high 

constitutive expression in a wide variety 

of mammalian cell types. 

Due to two multiple cloning sites on 

either side of the reporter gene, N- or 

C-terminal fusion proteins can easily 

pPK-CMV-F2 (N-GFP) has a GFP coding 

sequence without a stop codon inframe 

in the MCS. The gene of interest can 

therefore be expressed with GFP at the 

N-terminus. 

pPK-CMV-F3 (C-Luc) has a Luciferase 

coding sequence with a stop codon in- 

pUC ori 

pPK-CMV Fusion 

Vector 

HSV pA 

Kan/Neo 

AmpP 

SV40p 


and Silencing 

be generated. In addition, each vector 

frame in the MCS. The gene of interest 

Fig. 3: The pPK-CMV Fusion Vectors are 

contains a combined kanamycin/neo- 

can therefore be expressed with Lu- 

extremely versatile, and their compact 

mycin resistance gene, which allows for 

ciferase at the C-terminus. 

size insures high transfection efficiency. 


pPK-CMV-F1 Fusion Vector (C-GFP) 20 µg PK-MB-P003400 

pPK-CMV-F3 Fusion Vector (C-Luc) 20 µg PK-MB-P003500 

pPK-CMV-F2 Fusion Vector (N-GFP) 20 µg PK-MB-P013400 

pPK-CMV-F4 Fusion Vector (N-Luc) 20 µg PK-MB-P013500 


Please see chapter 1 for luciferase, beta-Gal, and GFP reporter assays.

66 


4.2 StarGate Cloning Systems 

Applications 

Convenient gene cloning in expression 

vectors optimized for different hosts 

Easy screening for optimal tags, 

promoters, and host systems 

Site-specific modification of a target 

gene 

Flexible fusion cloning 

Features 

E. coli, mammalian, yeast, and 

baculovirus vectors available 

Numerous vectors with different tags 

and promoters available 

Highly efficient one-tube reaction 

Extremely short combinatorial sites 

Blue/white screening 

Easy generation of gene mutations 

and parallel automated mutagenesis 

possible 

Cost-effective 

StarGate is a very efficient system for 

cloning, expressing, mutating, and fusing 

genes or gene fragments. It allows easy 

gene transfer among a variety of highlevel 

expression vectors, which have been 

optimized for diverse host systems. 

4.2.1 StarGate Combinatorial Cloning System 


and Silencing 

Efficient procedures for generating functional 

recombinant proteins or protein 

complexes are of key importance in stateof-the-art 

life sciences. A large range of 

tools such as different promoters, tags 

and host systems are available for expressing, 

purifying, detecting, or immobilizing 

recombinant proteins. 

Due to the diverse nature of proteins, it 

is impossible to predict which combination 

of these tools will perform best. 

PromoKine now provides the StarGate 

Combinational Cloning System to systemize 

and accelerate the initial search for the 

most successful tools. The StarGate system 

offers fast and convenient subcloning 

of an arbitrary target gene of interest 

in many different vectors, and it allows 

the systematic combination of promoters, 

tags, and other genetic elements. 

Moreover, an easy site-specific mutagenesis 

of this target gene is possible prior to 

subcloning it in the desired host-specific 

vector. 

The gene of interest is initially cloned into 

a Donor Vector to simultaneously fuse 

it with many different genetic surroundings 

via transfer into Acceptor Vectors 

to generate Destination Vectors. Each 

Acceptor Vector provides optimized conditions 

for the respective application, e.g., 

host-specific promoters, purification tags, 

or signal sequences for protein secretion 

into the culture medium. This allows for 

efficient expression of the target protein 

with useful features in different hosts. 

Because the Donor and Acceptor Vectors 

have extremely short combinatorial sites 

(4 bases only) and correlating features, 

the exact same protein sequence can be 

expressed in different hosts with minimal 

modification to the gene of interest. 

PCR 

StarCombinase1 TM 

GENE 

GENE 

One-Tube Reaction 

GENE 

Primer 

Entry Vector 

Donor Vector 

An 

A3 

A2 

StarCombinase2 TM Acceptor Vector A1 

PCR Fragment 

+ 

Recognition sites 

Opened Entry Vector 

Fig. 4 Left: Principle of StarGate Combinatorial 

Cloning: 


The gene of interest is amplified 

by PCR using a set of primers with 

short StarGate combinatorial sites 

Dn 

at the 5´ end. 

D3 

GENE 

D2 

The PCR product, StarCombinase, and a 

D1 

Destination Vector 

ready-to-use linear and entry vector are 

mixed. In a fast one-tube combinatorial 

reaction, the PCR product is introduced 

in the entry vector generating the donor 

vector. 

After propagating the Donor Vector in E. coli the gene of interest can be subcloned 

into different Acceptor Vectors with the desired features, like host-specific promoters 

or specific tags. To create the Destination Vector the Donor Vector with the gene of 

interest is mixed with StarSolutions and the Acceptor Vectors. In a fast, one-tube combinatorial 

reaction, the PCR product is introduced in the respective Acceptor Vectors. 

The resulting Destination Vectors are then transformed into the corresponding host 

cells for further applications (e.g. protein purification). 

Right: E. coli clones harbouring the desired Destination Vector with the gene of interest 

can be easily detected using blue/white selection. 

GOI 

Donor Vector 

Kanamycin selection 

Combinatorial sites 

Promoter 

GOI 

Destination Vector 

lac P/Za 

Acceptor Vector 

StarCombinase2 TM 

binding site 

Lac Z α expression 

blue colonies 

Ampicillin selection 

No Lac Z α expression 

white colonies 

Ampicillin selection


The core element of the system is the 

site-specific combinatorial enzyme Star- 

Combinase , that is included in the Star- 

Solutions and makes cloning fast, easy, 

and efficient. Optimal cloning efficiency 

is ensured by a directed reaction. 

The StarGate technology also provides 

easy generation of fusion proteins and 

a versatile mutagenesis system with the 

StarPrimerD’Signer software. 

The ready-to-use kits contain all required 

components to subclone the gene of interest 

and - if desired - perform a preceding 

site-directed mutagenesis or generate 

different modes of target gene fusions. 

4.2.2 StarGate Mutagenesis System 

The StarGate Combi Entry Cloning Set 

Fig. 5: Principle of StarGate 

also provides a convenient tool for site- 

Mutagenesis: 

specific modification of a target gene 

Mutation 

Two parts of the gene of interest are 

and subsequent cloning of the modified 

GENE 

amplified by PCR using two designed 

gene (StarGate Mutagenesis System). 

The desired mutation can be inserted 

2 x PCR 

Primer 

primer sets which overlap at the location 

where a mutation should be in- 

within a sequence stretch of up to 80 bp. 

troduced. Both primer sets have short 

The procedure is similar and fully com- 

StarGate combinatorial sites at the 5´ 

patible to the StarGate Combinatorial 

Cloning System with the exception that 

StarCombinase TM 

Open Entry Vector 

end. The two PCR products, StarCombinase, 

and ready-to-use linear and Entry 

two PCRs have to be performed instead 

Vector pENTRY-IBA are mixed. In a fast 

of a single PCR. The two primer sets can 

one-tube combinatorial reaction the 

easily be designed with the StarPrimer 

D’Signer Software, which is included in 

the kit. 

To design the primers, the sequence of 

the gene of interest (as present in the 

template) as well as the desired mutated 

gene sequence simply have to be pasted 

into the respective StarPrimer D’Signer 

sequence windows. The software then 

displays the required primer sequences. 


Mutation 

GENE 

Donor Vector 

PCR products are introduced in the entry 

vector generating the Donor Vector 

which contains the mutated gene. After 

propagating the Donor Vector in E. coli 

the mutated gene of interest can be 

subcloned into different Acceptor Vectors 

using the StarGate Combinatorial 

Cloning System. After transformation, E. 

coli clones harbouring the desired Donor 

clone with the mutated gene of interest 


and Silencing 

can be easily detected using blue/white 

selection. 

StarGate Combi Entry Cloning Set 

StarGate Transfer Reagent Set 

Acceptor Vectors 

The StarGate Combi Entry Cloning Set 

(20 reactions) includes the Entry Vector 

pENTRY-IBA51, StarSolutions M1 - M3 

for 20 reactions, For/Rev Seqencing 

Primers, competent E. coli Top10 cells 

(for 20 transformations), a DNA ladder 

(DNA Ruler), and the StarPrimer 

D’Signer Software. 

The StarGate Combi Newcomer Set is 

a very cost-effective offer for scientists 

starting to work with StarGate. It includes 

the StarGate Combi Entry Cloning 

Set as well as the StarGate Transfer 

Reagent Set. 

The StarGate Transfer Reagent Set is 

used to transfer the genes of interest 

from the Donor Vector directly into different 

Acceptor Vectors. You can choose 

from our wide range of sophisticated 

Acceptor Vectors specifically designed 

for your requirements. The set includes 

StarSolutions containing StarCombinase 

for 20 reactions. 

You can choose from a wide range of differing 

StarGate Acceptor Vectors specifically 

designed for your required application. 

The Acceptor Vectors are available 

separately and come ready-to-use for 5 

transfer reactions. For a detailed description 

of the currently available StarGate 

vectors please see: 

www.promokine.info/vectors

68 


4.2.3 StarGate Fusion Cloning System 

Applications 

troducing an internal ribosomal entry site 

(IRES), a Shine-Dalgarno sequence, or an 

StarGate Fusion Cloning Set 2 

(SD1) 

Generation of fusion proteins 

animo acid linked sequence between the 

Polycistronic gene expression in 

two genes. This enables the construction 

The StarGate Fusion Cloning Set 2 (SD1) 

mammalian cells 

of polycistronic genes, artificial operons, 

has a Shine-Dalgarno (SD) sequence for 

Construction of artificial operons for 

or fusion proteins respectively. 

the construction of artificial operons in E. 

multiple gene expression in bacterial 

Since StarGate Fusion Cloning can be 

coli. The Set includes a StarGate Fusion 

cells 

performed repeatedly, it is even possible 

Reagent Set (pENTRY-IBA51; 5 re- 

Gene function and protein interaction 

to generate large constructs consisting of 

actions), Downstream Fusion Vector 

studies 

several genes. 

pCFUSE-IBA11 (5 reactions), StarSolutions 

F1 to F6 including Star Combinase 

Features 


(IRES1) 

(for 5 fusions), Upstream Fusion Vector 

pNFUSE-IBASD11 (5 reactions), and 

Easy, fast, and flexible generation of 

competent E. coli TOP10 cells (for 15 

stable fusion proteins 

The StarGate Fusion Cloning Set 1(IRES1) 

transformations). 

Compatible with the StarGate 

Combinatorial Cloning System 

Highly efficient one-tube reactions 

provides an internal ribosomal entry site 

(IRES) for polycistronic gene expression 

in mammalian cells from one expres- 


(LINK1) 

Easy handling 

sion vector. The set includes a StarGate 

Fusion Reagent Set (pENTRY-IBA51; 5 

The StarGate Fusion Cloning Set 3 

StarGate Fusion Cloning is an optional 

reactions), Downstream Fusion Vector 

(LINK1) provides an amino acid linker 

intermediate step between the StarGate 

pCFUSE-IBA11 (5 reactions), StarSolu- 

(GSGGGSGGGS) for the generation of 


and Silencing 

Entry Reaction and the Transfer Reaction. 

It allows easy and fast fusion of 

two genes of interest present in separate 

Donor Vectors by performing two sequential 

StarGate Subcloning Reactions. 

In addition, it offers the possibility of in- 

tions F1 to F6 including StarCombinase 

(for 5 fusions), Upstream Fusion Vector 

pNFUSE-IBA-IRES11 (5 reactions), and 

competent E. coli TOP10 cells (for 15 

transformations). 

fusion proteins. The Set includes StarGate 

Fusion Reagent Set (pENTRY-IBA51; 5 

reactions), Downstream Fusion Vector 

pCFUSE-IBA11 (5 reactions), StarSolutions 

F1 to F6 including StarCombinase 

for 5 fusions, Upstream Fusion Vector 

pNFUSE-IBA-LINK11 (5 rxns), and competent 

E. coli TOP10 cells (for 15 transformations). 

lac P/Za 

Upstream Fusion 

Vector 


lac P/Za 

Gene 1 

Upstream Fusion 

Vector Gene 1 

Gene 1 

Donor Vector 

Gene 1 


G1 

link 

G2 

Donor Fusion 

Vector 

Gene 1/Gene 2 

Gene 2 

Donor Vector 

Gene 2 


Gene 2 

Downstream 

Fusion Vector Gene 2 

lac P/Za 

Entry Vector 


lac P/Za 

Downstream 

Fusion Vector 

Fig. 6: Principle of StarGate Fusion 

Cloning: The procedure starts with two 

StarGate Donor Vectors containing the 

genes or gene fragments to be fused. 

Through two separate subcloning reactions, 

the two target genes are transferred 

to the respective upstream Fusion 

Vector pNFUSE-IBA that contains the 

intergenic region (IRES, Shine-Dalgarno, 

or an amino acid linker) and to the common 

downstream Fusion Vector pC- 

FUSE-IBA11. This is done in a fast onetube 

combinatorial reaction and results 

in the generation of the Upstream and 

Downstream Fusion Vectors containing 

Gene 1 and Gene 2. After propagating 

the two vectors in E. coli the genes in the 

upstream and downstream fusion vectors 

are assembled in the Entry Vector 

(pENTRY-IBA-51). This is again done in 

a fast one-tube combinatorial reaction. 

The fused genes with the desired intergenic 

region are now ready to be subcloned 

into any of the StarGate Acceptor 

Vectors. If necessary, the procedure can 

be repeated to add more genes or gene 

fragments.


Standard 

Entry Reaction 

Mutagenesis 

Entry Reaction 

Combi Entry Cloning Set 

Donor 

Vector 

Fusion Cloning 

StarGate Fusion 

Cloning Sets 

Acceptor 

Vectors 

A1 A2 A3 An 

Transfer Reaction 

StarGate Transfer 

Reagent Set 

Destination 

Vectors 

D1 D2 D3 Dn 


of choice 


and Silencing 

Different Hosts 

Fig. 7: Overview of the StarGate Cloning System 


StarGate Combi Entry Cloning Set 20 rxn PK-MB-1608-000 

StarGate Combi Newcomer Set 20 rxn PK-MB-1608-996 

StarGate Transfer Reagent Set 20 rxn PK-MB-1603-001 

StarGate Fusion Cloning Set "IRES1" 5 rxns PK-MB-1607-001 

StarGate Fusion Cloning Set "SD1" 5 rxns PK-MB-1607-002 

StarGate Fusion Cloning Set "LINK1" 5 rxns PK-MB-1607-003 

Competent E. coli TOP 10 cells 20 rxns PK-MB-1600-020 

Acceptor Vector 5 rxns PK-MB-P4XXX 


The StarGate Cloning Systems are distributed for IBA GmbH, Germany

70 


4.3 Gene Silencing 

4.3.1 GeneOFF shRNA Vector Kits 

Applications 

High-level expression of shRNA 

for gene silencing 

Features 

Simple vector design and linear vector 

format for quick and convenient 

cloning 

G418 resistance gene for selection of 

stable cell lines 

Small vector size for efficient 

transfection 

Available with GFP reporter gene to 

determine transfection efficiency 

The GeneOFF shRNA Vectors have been 

developed to efficiently induce RNA interference 

(RNAi) by expressing small 

hairpin RNAs (shRNAs) in vitro or in 

vivo which are able to suppress distinct 

target genes. This is achieved by cloning 

an shRNA coding sequence of 45-50 

nucleotides into an RNA polymerase III 

expression cassette. After transfection, 

the shRNA sequence is expressed as a 

fold-back stem-loop structure that is 

subsequently processed in the cell into 

functional siRNAs. The GeneOFF pPK- 

H1 and pPK-U6 shRNA Vectors contain 

the optimized U6- or H1-RNA polymerase 

III promoters, allowing optimal 

expression in a wide variety of cell types. 

All GeneOFF shRNA Vectors contain a 

neomycin resistance gene for convenient 

selection of stable cell lines. In addition, 

there are vector variants available which 

provide GFP reporter genes to allow 

easy tracking of vector transfection efficiency. 

For information about our siRNA transfection 

reagents see chapter 5. 

Fig. 8: Plasmid maps of the four 

Afl III 

pPK shRNA vectors without (left) and 


and Silencing 

HSV TK 

Poly A 

pUC ori 

Kan/Neo 

pPK-H1 

(2921 bp) 

pAmp/pSV40 

pH1 promoter 

BamHI 

siRNA 

Cloning site 

EcoRI 

HSV TK 

Poly A 

Kan/Neo 

pUC ori 

pPK-H1-GFP 

(5318 bp) 

pAmp/pSV40 

pCMV 

pH1 

GFP 

Poly A 

EcoRI 

BamHI 

siRNA Cloning site 

NotI 

with (right) a GFP reporter gene. 

Afl III 

HSV TK 

Poly A 

pUC ori 

HSV TK 

Poly A 

pUC ori 

pCMV 

pU6 promoter 

Kan/Neo 

pPK-U6 

(3087 bp) 

pAmp/pSV40 

BamHI 

siRNA 

Cloning site 

EcoRI 

Kan/Neo 

pPK-U6-GFP 

(5318 bp) 

pAmp/pSV40 

pU6 

GFP 

Poly A 

EcoRI 

BamHI 

siRNA Cloning site 

NotI 


GeneOFF pPK-H1 shRNA Vector Kit 25 rxn PK-MB-P100100 

GeneOFF pPK-H1-GFP shRNA Vector Kit 25 rxn PK-MB-P100300 

GeneOFF pPK-U6 shRNA Vector Kit 25 rxn PK-MB-P600100 

GeneOFF pPK-U6-GFP shRNA Vector Kit 25 rxn PK-MB-P600300 



4.3.2 GeneOFF siRNA Generation Kit 

Applications 

Easy and economic generation of 

functional siRNAs 

Features 

Produces multiple siRNA species – 

thus greatly improving the success of 

silencing a target gene 

Many gene regions can be screened 

for silencing 

Kit includes the proven PromoFectin- 

siRNA Transfection Reagent 

The PromoKine GeneOFF siRNA Generation 

Kit provides easy and cost-effective 

generation of a large number of functional 

siRNAs from full-length target 

genes in vitro. The GeneOFF siRNA Genaration 

Kit combines rapid, high-yield 

synthesis of dsRNA (using T7 in vitro 

transcription) and extremely complete 

cleavage of these dsRNA templates into 

22-bp siRNAs by an ultra-active form 

of human Recombinant Dicer Enzyme. 

The kit contains sufficient reagents for 

generating a pool of small interfering 

RNAs (siRNAs) from up to 5 different 

genes and for 50 transfections in 24-well 

plates. It includes a T7 Transcription Kit 

for 5 transcription reactions, 50 units of 

Recombinant Dicer Enzyme, RNA purification 

columns and PromoFectin-siRNA 

for 50 transfections (see information 

about PromoFectin-siRNA in chapter 

5). PromoKine also provides an effective 

RNase inhbitor to protect siRNA from 

degradation. siProtect is non-toxic to 

cells and very stable under a broad range 

of siRNA transfection conditions. It is 

steril and free of DNases. 

Fig. 9: Principle of generating a pool 

of siRNAs using the GeneOFF siRNA 

Generation Kit. 

T7 promoter 

(Sense) 

T7 promoter 

(Sense) 

Sense RNA 

Target Sequence 

PCR 

T7 promoter 

(Anti-sense) 

Transcribe with 

T7 RNA polymerase 

Anneal and cleanup 

Anti-Sense RNA 

(Anti-sense) 

T7 promoter 

Digestion using recombinant 

Dicer TM enzyme and clean up 



and Silencing 

GeneOFF siRNA Generation Kit 1 kit PK-MB-T510001 

siProtect RNase Inhibitor 100 units PK-MB-T520100 



5 

The ability to introduce foreign nucleic acids into eukaryotic 

cells has become a powerful tool in studying and controlling 

gene expression. Cloned genes can be transfected into cells for 

biochemical characterization, mutational analyses, investigating 

gene regulatory elements, and producing specific proteins. Cell 

transfection promotes the rapid advancement of our knowledge 

of genetic regulation and protein function.

74 


5 


5.1 PromoFectin Transfection Reagents ..... 75 

5.2 Magnet Assisted Transfection (MATra) ... 79 

5.3 Transfection Accessories .............. 83

5 Cell Transfection 75 

5.1 PromoFectin Transfection Reagents 

Applications 

Efficient transfection of many cell 

types including primary cells 

Suited for plasmids, oligonucleotides, 

siRNA, and polypeptides 

For stable and transient transfections 

Features 

Superior transfection efficiency 

Fast procedure and easy handling 

without medium change 

Compatible with serum and 

antibiotics 

Good cell survival and viability after 

transfection 

PromoFectin 

PromoFectin is a series of reagents for 

highly efficient and reproducible transfection 

of a variety of adherent and 

non-adherent cells that can be used to 

transfect nucleic acids or proteins. PromoFectin 

comes either as a broad-range 

reagent or as specialized reagents for 

specific cell types and biomolecules. 

The standard, broad-range PromoFectin 

is a non-liposomal transfection reagent 

based on a water-soluble, cationic polymer. 

It is free of animal-derived components 

and shows very low cytotoxicity. 

PromoFectin ensures highly effective 

delivery of plasmids, oligonucleotides, 

and siRNA into a variety of adherent 

and non-adherent cell types with a high 

degree of reproducibility. Since its development, 

PromoFectin has been used 

successfully with many problematic cell 

lines and sensitive primary cells. 

Fig. 1: Primary fibroblasts (NHDF cells, 

PromoCell) transfected with the GFP 

reporter vector pPK-CMV-R4 (see 

chapter 5) using PromoFectin. 

Nucleic acid 

Cytoplasm 

Nucleus 

Fig. 2: PromoFectin assembles nucleic 

acids into compact structures that bind 

to the cell surface and are delivered 

into the cell by endocytosis. In the 

endosome, PromoFectin acts as a proton 

sponge, buffering the endosomal 

pH that leads to pH inhibition of endosomal 

nucleases. Then, continuous 

proton influx leads to osmotic swelling 

and rupture of the endosome - allow- 

PromoFectin 

Cationic 

complexes 

Endocytosis 

Endosome 

Proton 

sponge 

ing for almost complete release of the 

nucleic acids into the cytosol. Moreover, 

PromoFectin also favours DNA 

trafficking to the cell nucleus – a property 

that is particularly useful for trans- 

5 Cell Transfection 

fecting slow-dividing or resting cells. 

Nucleic acid 

in 150 mM NaCl 

solution 

Fig. 3: The PromoFectin transfection 

procedure is fast and easy. Add 

PromoFectin to the nucleic acid solution, 

mix, incubate for 15 minutes, add 

growth medium (which can contain 

serum and antibiotics), and pipette 

PromoFectin 

Incubate 

30 minutes at RT 

Incubate 24-28 h. 

Then, measure 

protein expression. 

the complexes onto the cells. The cells 

are then incubated for further analysis. 

No post-transfection removal of complexes 

or medium change/addition is 

necessary.

76 


Fig. 4: Comparison of transfection efficiencies for Promo- 

Fectin and three other common transfection reagents recommended 

for primary cells. Primary human fibroblasts 

(NHDF cells; PromoCell catalog number C-12300) cultured 

in 24-well plates were transfected with a luciferase 

reporter plasmid*. The appropriate protocols recommended 

by the manufacturers were used for transfection 

with PromoFectin and the competing reagents. All transfections 

were performed in triplicate (values normalized to 

1 mg protein). 

* See chapter 4 for reporter plasmids and chapter 1 for reporter assays. 

PromoFectin-HUVEC 

PromoFectin-Hepatocyte 

PromoFectin-Macrophage 

PromoFectin-HUVEC is a non-liposomal 

PromoFectin-Hepatocyte is a ligand- 

PromoFectin-Macrophage has been de- 

PromoFectin derivative optimized for 

conjugated, non-liposomal derivative of 

veloped for optimal transfection of the 

the transfection of primary endothelial 

PromoFectin specialized for hepatocyte 

known hard-to-transfect macrophages 

cells such as HUVEC 1 and HDMEC 2 . It 

transfection (e.g., primary human and 

(e.g. primary human macrophages ob- 

shows very little cytotoxicity and enables 

murine hepatocytes, hepatocyte cell 

tained from blood monocytes) and mac- 

very high transfection rates also with 

lines, and hepatocarcinoma cells). The 

rophage-derived cell lines such as RAW 


fragile, sensitive cell types. Once inside 

the cell, PromoFectin’s unique “proton 

sponge” property provides an excellent 

escape mechanism for DNA particles to 

the cytoplasm. 

reagent/nucleic acid complexes bind 

specifically to surface receptors on the 

target cells leading to extremely high 

transfection rates. 

264.7. PromoFectin-Macrophage is a 

mannose-bearing variant of the standard 

PromoFectin reagent. It binds to cells 

expressing mannose-specific membrane 

receptors - such as macrophages - leading 

to enhanced internalization of the 

DNA complexes. 

Fig. 5: Primary human endothelial cells 

Fig. 6: Primary human hepatocytes 

Fig. 7: Human monocyte-derived mac- 

(HUVECs) transfected with the reporter 

transfected with the reporter vector 

rophages were transfected with a galac- 

vector pPK-CMV-R4 (see chapter 4) 

pPK-CMV-R4 (see chapter 4) using 

tosidase-expressing plasmid after 7 days 

using PromoFectin-HUVEC. Nuclei 

PromoFectin-Hepatocyte. 

of culture in the presence of 100 U/ml 

counterstained with DAPI. 

GM-CSF and serum-containing medium 

1 

Human Umbilical Vein Endothelial Cells 

2 

Human Dermal Microvascular Endothelial Cells 

in 24-well plates.


Fig. 8: Comparison of transfection efficiencies for PromoFectin-HUVEC, 

PromoFectin, and three other common transfection 

reagents recommended for primary cells. Primary 

HUVEC cells (PromoCell catalog number C-12200) cultured 

in 24-well plates were transfected with a luciferase reporter 

plasmid*. The appropriate protocols recommended by the 

manufacturers were used for transfection with PromoFectin 

and the competing reagents. All transfections were performed 

in triplicate (values normalized to 1 mg protein). 

* See chapter 4 for reporter plasmids and chapter 1 for reporter assays. 

PromoFectin-Neuron 

reagents in comparison studies and 

a wide variety of cell types, including 

requires less DNA. PromoFectin-Insect 

problematic cell lines and primary cells. It 

PromoFectin-Neuron is a cationic, poly- 

demonstrates broad vector delivery ca- 

enables optimal gene silencing with low 

mer-based transfection reagent created 

pability, excellent reproducibility, and 

cytoxicity. 

for the transfection of primary neuronal 

cells, glial cells, and neuronal cell lines. 

high expression levels for small- or largescale 

protein production. 

PromoFectin-Polypeptide 

Cells take up the polymer/DNA complex 

through endocytosis, where the polymer 

PromoFectin-CellLine 

PromoFectin-Polypeptide is a novel li- 

is biodegraded into non-toxic molecules. 

Compared to viral methods, PromoFectin-Neuron 

allows less complicated and 

faster nucleic acid delivery with high 

transfection rates. 

PromoFectin-CellLine is a cationic, lipidbased 

reagent developed to obtain optimal 

transfection results with several 

widely used cell lines (CHO, HeLa, COS, 

posomal reagent created for the highly 

efficient and fast delivery of proteins and 

polypeptides (e.g. blocking peptides and 

labeled antibodies) into a wide variety of 

cells. It captures protein molecules and 


PromoFectin-Insect 

293, NIH-3T3) at low costs. Its formulation 

and the provided protocol allow fast 

transports them into the cytosol of living 

cells while maintaining the protein’s 

and convenient cell transfection. 

structure and functionality. This allows 

PromoFectin-Insect is a unique, lipsomebased 

transfection reagent optimized for 

PromoFectin-siRNA 

researchers to study intracellular protein 

functions without the need for DNA 

nucleic acid delivery into insect cells, es- 

cloning and DNA transfection. 

pecially those derived from Spodoptera 

PromoFectin-siRNA is a cationic, lipid- 

PromoFectin-Polypeptide is also available 

frugiperda (e.g. Sf9 or Sf21). It out- 

based transfection reagent designed for 

in pre-coated single-use tubes providing 

performs other insect cell transfection 

the highly efficient delivery of siRNA into 

additional convenience and speed. 

Fig. 9: Primary neurons transfected with 

the reporter vector pPK-CMV-R4 (see 

chapter 4) using PromoFectin-Neuron. 

Fig. 10: Left: NIH-3T3 cells transfected with the GFP reporter plasmid pPK-CMV-R4 

(see chapter 4) using PromoFectin-CellLine. Right: CHO cells transfected with the 

GFP reporter plasmid pPK-CMV-R4 (see chapter 4) using PromoFectin-CellLine.

78 


n Some cell types successfully transfected with PromoFectin 

Adipocyte cells 

Endothelial-like cells 

Epithelial-like cells 

Fibroblast-like cells 

Hepatocyte-like cells 

Keratinocytes 

Pancreatic carcinoma cells 

Primary human pre-adipocytes 

BBMC; BAEC; HLMEC*, HUVEC*, H-MVEC, primary HUVEC*, primary HDMEC* 

2CSFMEo, 3LL, 56FHTE8o, 6CSFMEo, 9HTEo-, A549, AsPC-1, BxPC-3, CaCo2, Ca Ski, 

CFNPE90- CF, CFPEo, CFSMEo, CHO, D 407, DHD Pro.b, HeLa, Huh-7D12, IGROV1, 

MCF-7, MDA-MB435S, MDCK, MIAPaCa-2, MLE-12, MLE-15, MRC-5, Panc-1, 

PGHAM-1, SAOS-2, SiHa, SKOV3, U-2 OS 

BHK-21, COS-7, CV-1, HEK-293, L929, NIH 3T3, primary human skin fibroblasts 

(NHDF) 

BNL, CL 2**, HepG2**, Huh-7**, M-PAC, primary human, mouse and rat 

hepatocytes** 

Primary human keratinocytes 

BxPC-3, PanC-1 

Leukemia cells / lymphoblasts Daudi, Jurkat, K562, Molt 4 

Melanoma cells 

Myotubes / myoblasts / muscle cells 

Neuronal cells 

Chondrocytes 

Glioblastoma 

Blood cells 

B16-F10 

C2C12 

CLBPEC, Neuro2A, PC12, SH-SY5Y, SHEP, SK-N-MC, 

primary chicken embryonic neurons 

HCS-2/8 

U87 

Human macrophages***, monocyte-derived macrophages***, 

RAW 264.7***, THP-1*** 

* PromoFectin-HUVEC, ** PromoFectin-Hepatocyte, *** PromoFectin-Macrophage 


Please contact us or see www.promokine.info for information about new cell types that have been successfully transfected using 

PromoFectin. 


PromoFectin 0.1 ml 

0.5 ml 

1 ml 


0.1 ml 

0.5 ml 

PromoFectin-Hepatocyte 

0.1 ml 

0.5 ml 

PromoFectin-Macrophage 

0.1 ml 

0.5 m 

PromoFectin-Neuron 

0.15 ml 

0.75 ml 

5 x 0.75 ml 

PromoFectin-Insect 0.5 ml 

1 ml 

PromoFectin-CellLine 0.75 ml 

1.5 ml 

PromoFectin-siRNA 

0.2 ml 

0.75 ml 

5 x 0.75 ml 

PromoFectin-Polypeptide 

24 reactions 

24 Single Use Vials 

96 reactions 

96 Single Use Vials 

PK-CT-2000-10 

PK-CT-2000-50 

PK-CT-2000-100 

PK-CT-2000-HUV-10 

PK-CT-2000-HUV-50 

PK-CT-2000-HEP-10 

PK-CT-2000-HEP-50 

PK-CT-2000-MAC-10 

PK-CT-2000-MAC-50 

PK-CT-2000-NEU-015 

PK-CT-2000-NEU-075 

PK-CT-2000-NEU-375 

PK-CT-2000-InC-50 

PK-CT-2000-InC-100 

PK-CT-2000-CL-075 

PK-CT-2000-CL-150 

PK-CT-2000-RNA-50 

PK-CT-2000-RNA-200 

PK-CT-2000-RNA-1000 

PK-CT-2000-POL-24 

PK-CT-2000-POL-24Q 

PK-CT-2000-POL-96 

PK-CT-2000-POL-96Q 



5.2 Magnet Assisted Transfection (MATra) 

Applications 

Magnet Assisted Transfection 

Magnet Assisted Lipofection 

Transfection of adherent and suspension 

cells including primary cells 

Suited for plasmids, oligonucleotides, 

siRNA, and adenovirus/retrovirus 

transfection 

For stable and transient transfections 

Also suited for high-thoughput 

transfection 

Adherent Cells Suspension Cells Adherent Cells Suspension Cells 

MATra-A 

or MATra-si 

Reagent 

Use MATra-S 

Immobilizer to make 

the cells adherent. 

MA Lipofection Enhancer 

and lipofection 

reagent of choice 

Use MATra-S 

Immobilizer to make 

the cells adherent. 

Features 

Easy, fast, and reproducible procedure 

Functional with and without serum 

and antibiotics 

Very high transfection rates also with 

many hard-to-transfect cell types 

Up to several thousand fold increased 

transgene expression 

Very low amounts of nucleic acid 

necessary for transfection 

Magnet Assisted Transfection (MATra) is 

a very fast, easy-to-handle, and highly 

efficient technology for transfecting cells 

in culture. It can be used for cells from a 

broad variety of species. The major components 

of MATra are coated magnetic 

nanoparticles, which effectively bind either 

nucleic acids or liposomes. Using a 

special magnet plate, the nanoparticles 

with the bound nucleic acids or liposomes 

are rapidly drawn towards the cells and 

are deposited at a high concentration on 

the outer cell membrane. Afterwards, 

the cells endocytose the particles, which 

leads to a high transfection rate even 

with problematic cells. 

MATra 

nanoparticles 

DNA + nanoparticle 

complex 

DNA 

Adherent cells 

Transfected 

Cells 

Fig. 11: Magnet Assisted Transfection (MATra) principle. 

The MATra technology is distributed for IBA GmbH, Germany. For research use only! 

Two approaches are possible: 

Magnet Assisted Transfection using 

MATra-A or MATra-siRNA Reagent. 

Magnet Assisted Lipofection using 

the MA Lipofection Enhancer and a 

lipofection reagent of choice. 

Both techniques can be used with adherent 

cells as well as with suspension cells. 

For suspension cells, the cells have to be 

immobilized at the bottom of the wells 

to make use of the magnetic field. This 

can be achieved by either temporarily 

immobilizing the cells with MATra-S Immobilizer 

or by using modified culture 

vessels. MATra is also suited for highthroughput 

transfection assays using 

adapted protocols. 

Magnet Plate 

Magnet 

Transfected 

Cells 

MATra-A Reagent 

The MATra-A Reagent contains magnetic 

nanoparticles with an innovative 

polyelectrolyte coating, which effectively 

bind nucleic acids (e.g. plasmid DNA or 

oligonucleotides) by means of electrostatic 

interactions. Using this technique, 

nucleic acids are initially mixed with specifically 

coated magnetic nanoparticles. 

The magnetic particles loaded with the 

nucleic acids are then added to cells in 

culture and a strong magnetic force is 

applied beneath the cells. Exploiting this 

strong magnetic field, the full nucleic 

acid dose is rapidly drawn towards and 

delivered into the target cells on the bottom 

of the multi-well plate or cell culture 

flask. This results in very close contact 

between the cell membrane and the 

highly concentrated nucleic acid loaded 

magnetic complexes. MATra allows efficient 

transfection without disturbing 

the cell membrane architecture, causing 

chromosomal damage, or leaving a 

hole in the cell membrane as with other 

transfection technologies. The transfection 

rate consequently is clearly increased 

- also with difficult-to-transfect 

cells such as primary cells. In addition, 

the amounts of nucleic acids required 

for successful transfection can be significantly 

reduced, which in turn further 

reduces cytotoxicity. 

5 Cell Transfection

80 


Phase contrast 

GFP fluorescence 

Overlay 

Fig. 12: Mouse fibrosarcoma cells transfected with GFP plasmid using MATra. (Data kindly provided for IBA GmbH by Dr. Lutz 

Thon and Dr. Dieter Adam, Institute for Immunology, University Schleswig-Holstein, Kiel, Germany). 

MATra-siRNA Reagent 

Specifically designed small inhibitory 

RNA oligonucleotides (siRNAs) allow 

siRNAs are small interfering/inhibitory 

transient inhibition of gene expression in 

sequence-specific RNA molecules that 

vitro and in vivo. 

bind to a complementary sequence 

MATra-siRNA contains specifically coat- 

of a target mRNA, block translation of 

ed magnetic nanoparticles, which are 

that target mRNA, and initiate its deg- 

optimized to effectively bind and stabi- 

radation. Consequently, synthesis of 

lize siRNA. It provides optimal delivery 

the corresponding protein of interest is 

of siRNA into many different cell types 

prevented and the specific gene function 

enabling efficient and prolonged gene- 

Fig. 13: Human endometrial stromal 

is knocked-out. This process is termed 

silencing. 

cells transfected with fluorescein-labeled 

RNA interference or gene-silencing and 

Please see chapter 4.4 for more gene 

siRNA using MATra-siRNA Reagent. 

it is used to elucidate gene functions. 

silencing tools. 

(Data kindly provided for IBA GmbH by 

Dr. Birgit Gellersen, Endokrinologikum 


%GFP positive cells 

Hamburg, Germany). 

50 

40 

Fig. 14: Comparison of transfection efficiencies of MATra, 

PromoFectin-HUVEC, and three other common transfection 

reagents recommended for primary cells. Primary human 

dermal microvascular endothelial cells (HDMEC, PromoCell 

catalog number C-12210) cultured in 24-well plates were 

transfected with a GFP reporter plasmid*. The appropriate 

protocols recommended by the manufacturers were used for 

transfection with MATra, PromoFectin, and the competing 

reagents. All transfections were performed in triplicate. 

30 

20 

10 

MATra 


Reagent FG6 

Reagent S18 

Reagent LF 

* See chapter 4 for reporter plasmids.



The MA Lipofection Enhancer contains 

coated magnetic nanoparticles, which 

bind liposomes and any polycationic 

transfection reagents (e.g. PromoFectin) 

which in turn bind nucleic acids (e.g. 

plasmid DNA, oligonucleotides, siRNA). 

Using the MA Lipofection Enhancer, cells 

are transfected more efficiently than 

with liposomal or polycationic transfection 

reagents alone. 

MATra-S Immobilizer 

Suspension cells have to be made adherent 

first to perform Magnet Assisted 

Transfection (as well as many other conventional 

transfection procedures). The 

MATra-S Immobilizer contains coated 

magnetic nanoparticles, which bind 

suspension cells. Using a special magnet 

plate, the magnetic nanoparticles with 

the bound cells are rapidly drawn towards 

the bottom of the cell culture vessel, 

where they are immobilized. By combining 

the MATra-S Immobilizer with the 

other MATra reagents, suspension cells 

can be transfected conveniently. 

See www.promokine.info for a listing 

of more than 100 cell types 

successfully transfected using Magnet 

Assisted Transfection and Magnet 

Assisted Lipofection. 

Liposome 

Cell membrane 

Magnetic nanoparticles 

Fig. 15: Principle of Magnet Assisted 

Lipofection. The MA Lipofection 

Enhancer can be combined with any 

commercially available transfection 

reagent based on polycations or lipids. 

Response (expression level) 

200 

150 

MATra 

100 

Fig. 16: Magnet Assisted Transfection and lipofection in comparison. 

Luciferase expression was assayed after 24 hours. 

With MATra reagents, low vector doses are sufficient to obtain 

good results. 

50 

2. generation lipofection reagents 

1. generation lipofection reagents 

0.05 0.1 

0.15 

Dose (µg DNA) 


%GFP positive cells 

10 

Fig. 17: Comparison of transfection efficiencies of two lipofection 

reagents with and without MA Lipofection Enhancer. 

Primary human dermal microvascular endothelial 

cells (HDMEC, PromoCell catalog number C-12210) cultured 

in 24-well plates were transfected with a GFP reporter 

plasmid*. The appropriate protocols recommended by the 

manufacturers were used for transfection. All transfections 

were performed in triplicate. IBAfect liposomal transfection 

reagent is available at PromoKine on request. Please visit 

www.promokine.info for more information. 

8 

6 

4 

2 

IBAfect + 

MA Lipopfection 

Enhancer 

IBAfect 

Reagent LF + 

MA Lipopfection 

Enhancer 

Reagent LF 

* See chapter 4 for reporter plasmids.

82 


Magnet Plates 

To perform Magnet Assisted Transfection, 

specially designed neodymium 

magnet plates are necessary. PromoKine 

offers them in different sizes, optimized 

for different cell culture vessels. 

Universal Magnet Plate (8 x 13 cm): 

suitable for 6-well, 12-well, 24-well, 

48-well, or 96-well plates, T75 flasks, 60 

mm dishes etc. 

Universal Magnet Plate (26 x 26 cm): 

suitable for 6-well, 12-well, 24-well, 

48-well, or 96-well plates, T75 flasks, 

60 mm dishes, 100 mm dishes etc. 

24 Magnet Bar Plate (8 x 12 cm): 

optimized for 24-well plates. 

96 Magnet Bar Plate (8 x 12 cm): 

optimized for 96-well plates 

Also available: Magnet Bar Plate Sets. 

Fig. 18: Universal magnet plate (8 x 13 cm) with collection of different culture plates 

and MATra reagents. 

Fig. 19: 96 magnet bar plate (8 x 12 cm) 

with 96-well culture plate. 



MATra-A Reagent 

MATra-siRNA Reagent 


MATra-S Immobilizer 

Magnet Plate 

Set of four Magnet Bar Plates 

0.05 ml 

0.2 ml 

1 ml 

0.05 ml 

0.2 ml 

1 ml 

0.05 ml 

0.2 ml 

1 ml 

0.05 ml 

0.2 ml 

1 ml 

Universal Magnet Plate, 8 x 13 cm plate 

Universal Magnet Plate, 26 x 26 cm plate 

24 Magnet Bar Plate, 8 x 12 cm plate 

96 Magnet Bar Plate, 8 x 12 cm plate 

Four 24 Magnet Bar Plates 

Four 96 Magnet Bar Plates 

Two 24 and two 96 Magnet Bar Plates 

PK-CT-2001-005 

PK-CT-2001-020 

PK-CT-2001-100 

PK-CT-2021-005 

PK-CT-2021-020 

PK-CT-2021-100 

PK-CT-2003-005 

PK-CT-2003-020 

PK-CT-2003-100 

PK-CT-2002-005 

PK-CT-2002-020 

PK-CT-2002-100 

PK-CT-2011-000 

PK-CT-2012-000 

PK-CT-2006-000 

PK-CT-2004-000 

PK-CT-2008-000 

PK-CT-2009-000 

PK-CT-2010-000 


With the MATra Evaluation Sets for adherent or suspension cells, you can test the MATra technology with your target cell type. 

Please see our website www.promokine.info, or contact us directly for more information on this special trial offer.


5.3 Transfection Accessories 

5.3.1 GeneBooster 

Applications 

Enhancement of gene expression in 

transfected cells 

Features 

Up to 12 fold increased gene 

expression levels in diverse cell 

lines after transfection 

Compatible with all commonly used 

cell transfection reagents 

Fast and easy protocol 

Optimized conditions for popular 

cell lines 

Efficient for both transient and 

stable transfections 

The GeneBooster Reagents are chemical 

cocktails designed to significantly 

enhance gene expression levels in transfected 

cells. Three unique formulations 

have been developed for different cell 

types. Simply add GeneBooster reagent 

to the culture medium four hours after 

transfection and an increase in expression 

levels up to 12 fold can be achieved. 

The reagents can be used in combination 

with all commercially available 

DNA transfection reagents (including 

all PromoFectin Reagents). One vial of 

GeneBooster Reagent is sufficient for 

150 transfections using 1 ml transfection 

volume. 

The three GeneBooster Reagents are also 

available in a kit. 

Fig. 20: K562, HeLa-S3, MCF7, and P19 cells were transfected with the beta-Gal reporter plasmid pPK-CMV-R1 (see chapter 4). 

After 4 hours, the appropriate GeneBooster reagent was added to the cells which were stained 48 hours post transfection using a 

beta-Gal Staining Kit (see chapter 1) and examined under a microscope. 

5.3.2 Cell Clean 

Applications 

Highly efficient cleanup of 

transfected cells 

Features 

Efficient removal of extracellular lipid/ 

DNA complexes after cell transfection 

Non-toxic to cells 

· Fast and easy procedure 

Better downstream results post 

transfection 

CellClean is a unique washing buffer that 

has been designed to remove all complexes 

of DNA and cationic lipids that 

associate with cell surfaces during transfection. 

It shows no cytotoxicity and can 

distinguish between extracellular and 

intracellular plasmid DNA. When Cell- 

Clean is used as part of the cell washing 

procedure, more precise quantitation of 

the amount of DNA delivered into cells 

can be achieved. Moreover, it improves 

downstream results such as DNA stability, 

cytometry, and gene delivery studies. 

Fig. 21: Adherent cells were incubated with cationic lipid/DNA complexes for 4 hours 

and washed with PBS (lane 1) or CellClean (lane 2). Cells were lysed, the DNA extracted 

and analysed by agarose gel electrophoresis. Arrows show extracellular plasmid 

DNA that could be removed from cell surfaces when using Cell Clean. 



GeneBooster Reagent I 3 vials x 1.5 ml each PK-CT-2020-1 

GeneBooster Reagent II 3 vials x 1.5 ml each PK-CT-2020-2 

GeneBooster Reagent III 3 vials x 1.5 ml each PK-CT-2020-3 

GeneBooster Reagent Kit 3 vials x 1.5 ml each PK-CT-2020-0 

CellClean 100 ml PK-CT-1000-01 



6 

Antibodies have a broad range of applications like the detection 

of cellular structures as well as the specific identification, 

localization, and purification of intracellular and extracellular 

biomolecules such as proteins. They are also essential tools 

in describing cellular processes and for diagnostic purposes. 

Consequently, they are important for many research disciplines 

like apoptosis, signal transduction, neurobiology, virology, and 

tumor biology.

86 


6 


6.1 Mono- and Polyclonal Antibodies ....... 87 

6.2 Labeled Antibodies and Labeling Kits .... 91 

6.3 ELISA Kits ......................... 93 

6.4 Antigens and Cell Lysates ............ 106

6 Antibodies and ELISAs 87 

6.1 Mono- and Polyclonal Antibodies 

PromoKine offers a wide and continuously 

growing range of mono- and polyclonal 

antibodies against a variety of 

interesting molecules like cytokines and 

growth factors as well as many other 

important key targets in immunology, 

signal transduction, apoptosis, neurobiology, 

virology, tumor biology, autophagy, 

homeostasis etc. All antibodies are purified 

and highly active and can be used 

for diverse applications such as ELISAs, 

flow cytometry, functional studies, immunofluorescence 

microscopy, immunohistochemistry, 

immunoprecipitation or 

Westem blotting. The antibodies have 

been produced in several different hosts 

(e.g. rabbit, mouse, rat, goat) and show 

specific reactivities against antigens from 

different organisms (e.g. human, mouse, 

rat). For many of our antibodies, blocking 

peptides as well as cell and tissue lysates 

are available that can be used for functional 

studies or as positive controls (see 

5.3). Other antibodies are also available 

as conjugates with e.g. FITC or biotin. 

PromoKine lists several thousand primary 

antibodies on its website. Please visit 

www.promokine.info/antibodies to find 

your specific antibody of interest using 

our convenient search function. 

The respective product information and 

pricing are also available online. 

PromoKine provides a variety of 

antibodies for many different research 

topics: 

n Cell Signaling: Cytokines, Growth Factors, CD Antigens, soluble Receptors, and Ligands 

Numerous antibodies against Interleukins 

(ILs), Interferons (IFNs), Fibroblast 

Growth Factors (FGFs), Monocyte 

Chemotactic Proteins (MCPs), Macrophage 

Inflammatory Proteins (MIPs), 

Transforming Growth Factors (TGFs), 

Tumor Necrosis Factors (TNFs), Vascular 

Endothelial Growth Factors and 

Receptors (VEGFs, VEGFRs), Insulin- 

Like Growth Factors and Binding Proteins 

(IGFs, IGF-BPs), Platelet-Derived 

Growth Factors (PDGFs), CC Chemokine 

Receptors (CCRs), Bone Morphogenetic 

Proteins (BMPs), Matrix Metalloproteinases 

(MMPs), Neurotrophins (NTs), 

Stromal Cell-Derived Factors (SDFs), 

beta-Defensins (BDs), Eotaxins, diverse 

CDs, WNTs as well as Adiponectin, EGF, 

G-CSF, GM-CSF, M-CSF, HGF, EPO, Insulin, 

Leptin, Resistin, RANKL, RANTES, 

and many more targets. 

Please see www.promokine.info/antibodies/cell-signaling 

n Signal Transduction 

Numerous antibodies against PAK, TRAF, 

CTRP, IKK, IRAK, JNK1, CIKS, MADD, 

MAPKAP1, MyD88, Nanos, PTEN, 

SATB1, SHP2, SOCS1, STAT1, TSC, XBP 

and many more targets. 

Please see www.promokine.info/antibodies/signal-transduction 

n Apoptosis 

Numerous antibodies against Caspases, 

Calpains, Death Receptors (DRs), HDACs 

and HATs as well as Annexin V, AIF, 

CIDE, Cytochrome c, Daxx, DcR, DFF, 

DRAK, Fas and Fas Ligand, FLIP, ICAD, 

LAMP, NOD2, PDCD, PHAP, PIST, 

Survivin, TCCR, TRAIL, XAF, XIAP ZIPK 

and, many more targets. 

6 Antibodies and 

ELISAs 

APO-1, ASK1, Bax, Bcl-2, CAD, CARD, 

PUMA, RAIDD, RICK, Smac, SODD, 

Please see www.promokine.info/antibodies/apoptosis 

n p53 Network 

Antibodies e.g. against p53, p53DINP1/ 

SIP, p53R2, PARC, PERP, PID/MTA2, 

ChK2, MTBP. 

Please see www.promokine.info/antibodies/p53-network

88 

6 Antibodies and ELISAs 

n Neurobiology 

Numerous antibodies against APH1, 

BACE, BRSK, CAPS, CDNF, DISC1, FEZ, 

Grik, LGI, Neuritin, Neurotrypsin, Nicastrin, 

NK3R, NPAS3, PEN2, SAPAP, Slitrk, 

Syntaphilin, TMP, TOCA and many other 

targets. 

Please see www.promokine.info/antibodies/neurobiology 

n Stem Cell Research 

Numerous antibodies against AFX, Bad, 

DKK, Erk2, LRP-5/6, NFkB, PTEN, Smad, 

Sox, SSEA, Stat, Tau13, TLR, TRAF6 and 

many other targets. 

Please see www.promokine.info/antibodies/stem-cell-research 

n Cancer Research 

Numerous antibodies against APCs, 

BCAS, CUEDC, JMJD, OCIAD, SIPA1L, 

Cathepsins and many more targets. 

Please see www.promokine.info/antibodies/cancer-research 

n Virology 

Numerous antibodies against HIV and 

Chemokine Receptors (e.g. CCRs, 

CX3CR1, CXCR4, DC-SIGN, DDX-3, 

HIV-I and HIV-II Glycoproteins, TRIM5), 

Herpes (e.g. TNFRSF14/HVEM), SARS 

and SARS Receptors (e.g. ACE2, SARS E, 

SARS M, SARS Spike, SARS-NP), Anthrax 

(e.g. Anthrax EF, Anthrax LF, Anthrax 

PA, ATR/TEM8), West Nile Virus (e.g. 

WNV Core, WNV Env, WNV Matrix), 

Influenza (e.g. Influenza-A and Influenza-B), 

Avian Influenza (e.g. AFHA, H5N1 

Neuramidase and Hemagglutinin, H5N1 

NS1/NS2), Hepatitis (e.g. HCVNS3, 

HDV Hepatitis B), Epstein-Barr and Dengue 

Virus, (e.g. EBV, Dengue type 2) 

and virus-related targets (e.g. Rift Valley 

Fever Virus, UNG1, UNG2). 

Please see www.promokine.info/antibodies/virology 


ELISAs 

n Toll-Like Receptor Related 

Numerous antibodies against Toll-Like 

Receptors (TLRs) as well as ECSIT, MD-2, 

SIGIRR, TIRAP, TIRP, TOLLIP and many 

other targets. 

Please see www.promokine.info/antibodies/toll-like-receptor-related 

n Homeostasis 

Numerous antibodies against beta-Actin, 

Tumor Endothelial Markers (TEMs), 

BRCC, EVER, LIMP2, Nephrin, PTK7, 

STEAP, TNPO3, TRIM, TRT and many 

more targets. 

Please see www.promokine.info/antibodies/homeostasis


n Kinases & Phosphatases 

Numerous antibodies against Akt/PKB, 

ALK, ASK1, Erk, IKK, JKK, Lck, Mek, 

PAK, PKA, PKC and many other targets. 

Please see www.promokine.info/antibodies/kinases-and-phosphatases 

n Phospho-specific Antibodies 

Numerous antibodies against phosphorylated 

AFX, Akt, c-Jun, c-Myc, FAK, 

GSK-3, JKK, Lck, PKC, Stat, Tau and 

many other targets. 

Please see www.promokine.info/antibodies/phospho-specific-antibodies 

n Immunology 

Many antibodies e.g. against ADAP, 

Blimp-1, Carabin, MICA, ORAI, STIM, 

and TSLP. 

Please see www.promokine.info/antibodies/immunology 

n Innate Immunity 

Many antibodies e.g. against IPR1, LGP2, 

MDA5, PAP1, SLPI, VISA, ZBP1. 

Please see www.promokine.info/antibodies/innate-immunity 

n Autophagy 

Antibodies e.g. against Ambra1, ATG, 

IRGM. 

Please see www.promokine.info/antibodies/autophagy 


ELISAs 

n Subcellular Markers 

Antibodies e.g. against Apaf-1, Bax, Bid, 

Oct, diverse TLRs, Tubulin, Syndecan-4, 

VDAC, DKK1. 

Please see www.promokine.info/antibodies/subcellular-markers

90 


n Anti-Tag Antibodies 

Many antibodies e.g. against FLAG-Tag, 

GFP, RFP, GST-Tag, HA-Tag, His-Tag, 

Myc-Tag, and MBP. 

Please see www.promokine.info/antibodies/anti-tag-antibodies 

n Infectious Diseases 

Antibodies e.g. against Chlamydia LPS or 

Neisseria gonorrhea. 

Please see www.promokine.info/antibodies/infectious-diseases 

n Obesity 

Antibodies e.g. against ELOVL6, GPAT1, 

Lipe/HSL and UROP11. 

Please see www.promokine.info/antibodies/obesity 

n Miscellaneous Antibodies 

Numerous antibodies against Growth 

Hormone (GH), Immunglobulins, Lucife- 

rase, Macrophages, Plectin and many 

other targets. 

Please see www.promokine.info/antibodies/miscellaneous-antibodies 


ELISAs


6.2 Labeled Antibodies and Labeling Kits 

- 

n Labeled Secondary Antibodies 

PromoKine is offering secondary antibodies 

which are fluorescently labeled 

or conjugated to horseradish peroxidase 

(HRP), alkaline phosphatase (AP), or 

biotin. All antibodies are purified and 

highly active, and they can be used for 

diverse applications including ELISAs, immunofluorescence 

microscopy, immunohistochemistry, 

and Western blotting. 


Donkey Anti-Goat antibody (pAb), 

Fluorescein-coupled 

Donkey Anti-Goat IgG conjugated to Fluorescein 

(H&L) 

2 mg PK-AB718-9360 

Donkey Anti-Goat antibody (pAb), 

Texas Red-coupled 

Goat Anti-Chicken antibody (pAb), 


Donkey Anti-Goat IgG conjugated to Texas Red (H&L) 2 mg PK-AB718-9370 

Goat Anti-Chicken IgG conjugated to Texas Red (H&L) 2 mg PK-AB718-9170 

Goat Anti-Mouse antibody (pAb), 

AP-coupled 


Biotin-coupled 




HRP-coupled 

Goat Anti-Mouse IgG conjugated to Alkaline 

Phosphatase (H&L, minimum cross-reactivity) 

Goat Anti-Mouse IgG conjugated to Biotin 

(H&L, minimum cross-reactivity) 

Goat Anti-Mouse IgG conjugated to Fluorescein 


Goat Anti-Mouse IgG conjugated to Horseradish 

Peroxidase (H&L, minimum cross-reactivity) 

1 mg PK-AB718-9780 

2 mg PK-AB718-9740 

2 mg PK-AB718-9760 

2 mg PK-AB718-9720 



Goat Anti-Mouse IgG conjugated to Texas Red (H&L) 2 mg PK-AB718-9770 

Goat Anti-Rabbit antibody (pAb), 

AP-coupled 


Biotin-coupled 




HRP-coupled 

Goat Anti-Rabbit IgG conjugated to Alkaline 


Goat Anti-Rabbit IgG conjugated to Biotin 


Goat Anti-Rabbit IgG conjugated to Fluorescein 


Goat Anti-Rabbit IgG conjugated to Horseradish 


1 mg PK-AB718-9980 

2 mg PK-AB718-9940 

2 mg PK-AB718-9960 

2 mg PK-AB718-9920 



Goat Anti-Rabbit IgG conjugated to Texas Red (H&L) 2 mg PK-AB718-9970 


HRP-coupled 

Goat Anti-Rabbit IgG conjugated to horseradish 

peroxidase 

0.5 ml PK-AB577-6401 

Goat Anti-Rat antibody (pAb), 

AP-coupled 

Goat Anti-Rat antibody (pAb), 

HRP-coupled 

Goat Anti-Rat IgG conjugated to Alkaline Phosphatase 


Goat Anti-Rat IgG conjugated to Horseradish 


1 mg PK-AB718-9580 

2 mg PK-AB718-9520 

PF-XXX-Goat anti-Human IgG Conjugated to PromoFluor dye of choice 1 mg PK-PFXXX-AK-H1 


ELISAs 

PF-XXX-Goat anti-Mouse IgG Conjugated to PromoFluor dye of choice 1 mg PK-PFXXX-AK-M1 

PF-XXX-Goat anti-Rabbit IgG Conjugated to PromoFluor dye of choice 1 mg PK-PFXXX-AK-R1 

Rabbit Anti-Chicken antibody 

(pAb), AP-coupled 

Rabbit Anti-Chicken antibody 

(pAb), HRP-coupled 

Rabbit Anti-Goat antibody (pAb), 

AP-coupled 


HRP-coupled 

Rabbit Anti-Chicken IgG conjugated to 

Alkaline Phosphatase (H&L) 

Rabbit Anti-Chicken IgG conjugated to Horseradish 


Rabbit Anti-Goat IgG conjugated to Alkaline 


Rabbit Anti-Goat IgG conjugated to Horseradish 


1 mg PK-AB718-9180 

1.5 mg PK-AB718-9120 

0.5 mg PK-AB718-9380 

1 mg PK-AB718-9320 


PF-XXX- stands for the respective PromoFluor dye (e.g. PF-350, PF-405, etc.; see Chapter 3.1). 

Please see our website for an up-to-date list of available PromoFluor Labeled Antibodies.

92 




HRP-coupled 

Rabbit Anti-Mouse antibody (pAb), 

HRP-coupled 

Rabbit Anti-Goat IgG conjugated to horseradish 

peroxidase 

Rabbit Anti-Mouse IgG conjugated to horseradish 

peroxidase 

0.5 ml PK-AB577-6403 

0.5 ml PK-AB577-6402 

n Protein and Antibody Labeling Kits 

PromoKine also offers ready-to-use 

Protein and Antibody Labeling Kits. 

These innovative kits have been 

developed for convenient and highly efficient 

labeling of specific antibodies or 

target proteins with our superior Promo- 

Fluor dyes (see also Chapter 3). 




Kit for Labeling of Proteins/Antibodies 

with a Selected PromoFluor Dye 

Kit for Labeling of Proteins/Antibodies 

with a Selected PromoFluor Dye 

5 reactions PK-PF-LK-XXX-05 

10 reactions PK-PF-LK-XXX-10 

n Western Blotting Kits and Reagents 

PromoKine‘s ECL Chemiluminescence 

Detection Kit is a convenient, nonradioactive 

detection system containing 

all reagents required for highly sensitive 

chemiluminescent detection of immobilized 

proteins (Western blotting) or immobilized 

nucleic acids (Souuthern Blotting 

or Northern Blotting) conjugated with 

HRP (Horse Radish Peroxidase). This 

ready-to-use kit allows the detection of 

membrane immobilized specific antigens 

or nucleic acid sequences directly labeled 

with HRP or indirectly labeled with HRPtagged 

antibodies/streptavidin/biotin. 



ELISAs 

PromoKine ECL 

Chemiluminescence Detection Kit 

PromoKine Super-Block 

PromoKine Hybridization Solution 

Kit for Chemiluminescent Detection of HRP 

or HRP-labeled Biomolecules 

Blocking Reagent for Western, Northern & 

Southern Blotting 

Hybridization Solution for Northern & 

Southern Blotting 

120 ml 

500 ml 

1000 ml 

PK-MB902-500-120 

PK-MB902-500-500 

PK-MB902-500-1000 

10 g PK-MB902-805 

100 ml PK-MB902-889 


PF-XXX- stands for the respective PromoFluor dye (PF-405, PF-415, PF-488, PF-488LSS, PF-514LSS, PF-546, PF-555, PF-590, PF-633, PF-647, PF-670, PF- 700, PF-750, PF-770) 

Please see our website for an up-to-date list of available PromoFluor Labeling Kits.


6.3 ELISA Kits 

PromoKine offers a range of highly specific 

and sensitive ELISA kits that meet 

the highest quality standards. They can 

be used for reliable and reproducible 

detection and quantitation of numerous 

biomolecules in cell biology research. 

The 96 assay plate can either be used at 

once or a desired number of assays can 

be used because the wells in the assay 

plates can be detached. 

In addition, we offer ELISA Development 

Kits which are very economical in medium- 

to high-throughput applications. 

The kits are intended for experienced 

ELISA users and contain all required 

reagents to assay your target protein/ 

peptide in ten 96-well microtiter plates 

(approximately 1,000 assays). 

We continuously add new ELISA kits to our inventory. 

For an up-to-date product list please visit www.promokine.info/ELISAs 

n Cell Signaling: Cytokines, Growth Factors, Receptors and Ligands 


4-1BB / CD137 ELISA Kit, human Human soluble CD137 (4-1BB) ELISA 96 tests PK-EL-66556 

90K (Mac-2BP) ELISA Kit, human 

Human Macrophage Galactose-specific Lectin Binding 

Protein ELISA 

96 tests PK-EL-69196 

Adiponectin ELISA Kit, human Human Adiponectin ELISA 96 tests PK-EL-66635 

Angiogenin ELISA Kit, human Human Angiogenin ELISA 96 tests PK-EL-66645 

Angiopoietin-1 ELISA Kit, human Human Angiopoietin-1 ELISA 96 tests PK-EL-60015 

Angiostatin ELISA Kit, human Human Angiostatin ELISA 96 tests PK-EL-60090 

AXL ELISA Kit, human Human AXL ELISA 96 tests PK-EL-60080 

BAFF ELISA Kit, human Human B-cell Activating Factor (BLyS) ELISA 96 tests PK-EL-K9410 

BDNF ELISA Kit, human Human Brain Derived Neurotrophic Factor ELISA 96 tests PK-EL-66212 

BLC ELISA Kit, human Human B-Lymphocyte Chemoattractant ELISA 96 tests PK-EL-65240 

BMP-4 ELISA Kit, human Human Bone Morphogenetic Protein 4 ELISA 96 tests PK-EL-67314 



CD40L ELISA Kit, human Human soluble CD40 Ligand ELISA 96 tests PK-EL-63106 

CNTF ELISA Kit, human Human Ciliary Neurotrophic Factor ELISA 96 tests PK-EL-66210 

CRP ELISA Kit, human Human C-Reactive Protein ELISA 96 tests PK-EL-K9710s 

EGF ELISA Kit, human Human Epidermal Growth Factor ELISA 96 tests PK-EL-60170 

EGF-R (active) ELISA Kit, human 

EGF-R ELISA Kit, human 

Human soluble Epidermal Growth Factor Receptor 

(active) ELISA 

Human soluble Epidermal Growth Factor Receptor 

ELISA 



Eotaxin-2 ELISA Kit, human Human Eotaxin-2 ELISA 96 tests PK-EL-65330 


ELISAs 

EPO ELISA Kit, human Human Erythropoietin ELISA 96 tests PK-EL-60022 

sE-selectin / CD62E ELISA Kit, human Human soluble E-selectin (CD62E) ELISA 96 tests PK-EL-69216 

Fas ELISA Kit, human* Human Fas ELISA 96 tests PK-EL-69207L 

FGF-2 / FGF-b ELISA Kit, human Human Fibroblast Growth Factor basic (FGF-2) ELISA 96 tests PK-EL-60240 

FGF-2 / FGF-b ELISA Kit, human* Human Fibroblast Growth Factor basic (FGF-2) ELISA 96 tests PK-EL-60240L 

FGF-4 ELISA Kit, human Human Fibroblast Growth Factor 4 ELISA 96 tests PK-EL-60345 




* For cell and tissue lysate

94 



flt-3 Ligand ELISA Kit, human Human flt-3 Ligand ELISA 96 tests PK-EL-67110 

Fractalkine ELISA Kit, human Human Fractalkine ELISA 96 tests PK-EL-67510 

Galectin-3 ELISA Kit, human Human Galectin-3 (Mac-2) ELISA 96 tests PK-EL-69136 

G-CSF ELISA Kit, human Human Granulocyte Colony Stimulating Factor ELISA 96 tests PK-EL-60434 

GITR ELISA Kit, human Human GITR ELISA 96 tests PK-EL-60510 

GITR ELISA Kit, human* Human GITR ELISA 96 tests PK-EL-60510L 


ELISAs 

GM-CSF ELISA Kit, human 

Human Granulocyte/Macrophage Colony Stimulating 

Factor ELISA 


HCC-4 ELISA Kit, human Human Hemofiltrate CC-Chemokine 4 ELISA 96 tests PK-EL-67720 

HGF ELISA Kit, human Human Hepatocyte Growth Factor ELISA 96 tests PK-EL-64530 

HGF ELISA Kit, human* Human Hepatocyte Growth Factor ELISA 96 tests PK-EL-64530L 

sICAM-1 / CD54 ELISA Kit, human Human soluble Intercellular Adhesion Molecule 1 

(CD54) ELISA 


(CD102) ELISA 


(CD50) ELISA 




IFN-alpha ELISA Kit, human Human Interferon alpha ELISA 96 tests PK-EL-60586 

anti-IFN-alpha ELISA Kit, human Human Anti-Interferon alpha ELISA 96 tests PK-EL-69016 

IFN-gamma ELISA Kit, human Human Interferon gamma ELISA 96 tests PK-EL-60706 

IFN-gamma ELISA Kit, human* Human Human Interferon gamma ELISA 96 tests PK-EL-60707L 

IFN-omega ELISA Kit, human Human Interferon omega ELISA 96 tests PK-EL-60806 

IGF-BP1 ELISA Kit, human Human Insulin Like Growth Factor Binding Protein 1 

ELISA 


ELISA 


ELISA 


ELISA 





IGF-I ELISA Kit, human Human Insulin Like Growth Factor 1 ELISA 96 tests PK-EL-60840 

IL-1 alpha ELISA Kit, human Human Interleukin 1-alpha ELISA 96 tests PK-EL-61106 

IL-1 alpha ELISA Kit, human* Human Interleukin 1-alpha ELISA 96 tests PK-EL-61107L 

IL-1 beta ELISA Kit, human Human Interleukin 1-beta ELISA 96 tests PK-EL-61126 

IL-1 RA ELISA Kit, human Human Interleukin 1 Receptor Antagonist ELISA 96 tests PK-EL-61122 

IL-1 RA ELISA Kit, human* Human Interleukin 1 Receptor Antagonist ELISA 96 tests PK-EL-61122L 

IL-2 ELISA Kit, human Human Interleukin 2 ELISA 96 tests PK-EL-61206 

IL-2R ELISA Kit, human Human soluble Interleukin 2 Receptor ELISA 96 tests PK-EL-61216 




IL-6 ELISA Kit, human* Human Interleukin 6 ELISA 96 tests PK-EL-61607L 





IL-6R ELISA Kit, human Human soluble Interleukin 6 Receptor ELISA 96 tests PK-EL-61636 



IL-8 ELISA Kit, human* Human Interleukin 8 ELISA 96 tests PK-EL-61807L 



IL-12 (p40) ELISA Kit, human Human Interleukin 12 (p40) ELISA 96 tests PK-EL-62215 

IL-12 (p70) ELISA Kit, human Human Interleukin 12 (p70) ELISA 96 tests PK-EL-62216 









IP-10 ELISA Kit, human Human IFN Inducible Protein 10 (CXCL10) ELISA 96 tests PK-EL-65520 

Leptin ELISA Kit, human Human Leptin ELISA 96 tests PK-EL-68232 

LIF ELISA Kit, human Human Leukocyte Inhibitory Factor ELISA 96 tests PK-EL-60046 

LIF-R (gp190) ELISA Kit, human 

Human soluble Leukocyte Inhibitory Factor Receptor 

(gp190) ELISA 


Light ELISA Kit, human Human LIGHT (TNFSF14) ELISA 96 tests PK-EL-69167 

sL-Selectin ELISA Kit, human Human soluble L-selectin ELISA 96 tests PK-EL-69218 

MCP-1 ELISA Kit, human 

MCP-2 ELISA Kit, human 

Human Monocyte Chemotactic Protein 1 (CCL2) 

ELISA 

Human Monocyte Chemotactic Protein 2 (CCL8) 

ELISA 



M-CSF ELISA Kit, human Human Macrophage Colony Stimulating Factor ELISA 96 tests PK-EL-60442 

MDC ELISA Kit, human 

MIG ELISA Kit, human 

MIP-1 beta ELISA Kit, human 

MIP-1 delta ELISA Kit, human 

MIP-3 alpha ELISA Kit, human 

MIP-3 alpha ELISA Kit, human* 

Human Macrophage Derived Chemokine (CCL22) 

ELISA 

Human Monokine Induced by Interferon gamma 

(CXCL9) ELISA 

Human Macrophage Inflammatory Protein 1-beta 

(CCL4) ELISA 

Human Macrophage Inflammatory Protein 1-delta 

ELISA 

Human Macrophage Inflammatory Protein 3-alpha 

(CCL20) ELISA 


(CCL20) ELISA 






96 tests PK-EL-65866L 

MMP-1 ELISA Kit, human Human Matrix Metalloproteinase 1 ELISA 96 tests PK-EL-64102 




ELISAs 



96 






MPO ELISA Kit, human Human Myeloperoxidase (MPO) ELISA 96 tests PK-EL-K6631 

MSP ELISA Kit, human Human Macrophage Stimulating Protein ELISA 96 tests PK-EL-68556 

Myostatin ELISA Kit, human Human Myostatin ELISA 96 tests PK-EL-K1012 

NAP-2 ELISA Kit, human Human Neutrophil Activating Protein 2 (CXCL7) ELISA 96 tests PK-EL-64320 

NT-3 ELISA Kit, human Human Neurotrophin 3 ELISA 96 tests PK-EL-66425 

OPG ELISA Kit, human Human Osteoprotegerin (OPG) ELISA 96 tests PK-EL-63750 

OX-40 / CD134 ELISA Kit, human Human soluble CD134 (OX-40) ELISA 96 tests PK-EL-68166 

p185 (HER-2) ELISA Kit, human Human soluble p185 (EGF Receptor 2) ELISA 96 tests PK-EL-60196 

PAI-1 ELISA Kit, human Human Plasminogen Activator Inhibitor 1 ELISA 96 tests PK-EL-63790 


ELISAs 

PARC ELISA Kit, human 

Human Pulmonary & Activation-Regulated 

Chemokine ELISA 


PDGF-BB ELISA Kit, human Human Platelet Derived Growth Factor BB ELISA 96 tests PK-EL-63022 

PDGF-BB ELISA Kit, human* Human Platelet Derived Growth Factor BB ELISA 96 tests PK-EL-63022L 

sPECAM-1 / CD31 ELISA Kit, human 

Human soluble Platelet Endothelial Cell Adhesion 

Molecule 1 (CD31) ELISA 


PMN-Elastase ELISA Kit, human Human PMN-Elastase ELISA 96 tests PK-EL-69186 

sP-selectin / CD62P ELISA Kit, human Human soluble P-selectin (CD62P) ELISA 96 tests PK-EL-69220 

RANTES ELISA Kit, human Human RANTES (CCL5) ELISA 96 tests PK-EL-64130 

Resistin ELISA Kit, human Human Resistin ELISA 96 tests PK-EL-66670 

SCF ELISA Kit, human Human Stem Cell Factor ELISA 96 tests PK-EL-63120 

SDF-1 alpha ELISA Kit, human Human Stromal Cell Derived Factor 1-alpha ELISA 96 tests PK-EL-67220 

sgp130 ELISA Kit, human Human sgp130 ELISA 96 tests PK-EL-63150 

sPSGL-1 / CD162 ELISA Kit, human 

sRANKL (total) ELISA Kit, human 

sRANKL ELISA Kit, human 

Human soluble CD162 

(soluble P-selectin Glycoprotein Ligand 1) ELISA 

Human soluble Receptor Activator of NF-Kappa-B 

Ligand (total) ELISA 

Human soluble Receptor Activator of NF-Kappa-B 

Ligand ELISA 


96 tests PK-EL-K1016 

96 tests PK-EL-KB20452 

TGF-alpha ELISA Kit, human Human Transforming Growth Factor alpha ELISA 96 tests PK-EL-63535 

TGF-beta 1 ELISA Kit, human Human Transforming Growth Factor beta-1 ELISA 96 tests PK-EL-63506 

TGF-beta 2 ELISA Kit, human Human Transforming Growth Factor beta-2 ELISA 96 tests PK-EL-63508 

TIE-2 ELISA Kit, human Human TIE-2 ELISA 96 tests PK-EL-68028 

TIMP-1 ELISA Kit, human Human Tissue Inhibitor of Matrix Metalloprotease 1 

ELISA 

TIMP-2 ELISA Kit, human Human Tissue Inhibitor of Matrix Metalloprotease 2 

ELISA 



TNF-alpha (total) ELISA Kit, human Human Tumor Necrosis Factor alpha (total) ELISA 96 tests PK-EL-63708 

TNF-alpha ELISA Kit, human Human Tumor Necrosis Factor alpha ELISA 96 tests PK-EL-63707 

TNF-beta ELISA Kit, human Human Tumor Necrosis Factor beta ELISA 96 tests PK-EL-63736 





TNF-R (60 kDa) ELISA Kit, human Human soluble Tumor Necrosis Factor Receptor (60 

kDa) ELISA 

TNF-R (80 kDa) ELISA Kit, human Human soluble Tumor Necrosis Factor Receptor (80 

kDa) ELISA 



TNF-R II ELISA Kit, human 

t-PA ELISA Kit, human 

Human soluble Tumor Necrosis Factor Receptor Type 

2 ELISA 

Human Tissue-type Plasminogen Activator (t-PA) 

ELISA 



TPO ELISA Kit, human Human Thrombopoietin ELISA 96 tests PK-EL-65112 

TRAIL ELISA Kit, human Human TNF-Related Apoptosis Inducing Ligand ELISA 96 tests PK-EL-63606 

VAP-1 ELISA Kit, human Human soluble Vascular Apoptosis Inducing Protein 1 

ELISA 

sVCAM-1 / CD106 ELISA Kit, human Human soluble Vascular Cellular Adhesion Molecule 1 

(CD106) ELISA 

VEGF-A ELISA Kit, human 

VEGF-A ELISA Kit, human* 

VEGF-C ELISA Kit, human, human 

VEGF-R I ELISA Kit, human 

VEGF-R II ELISA Kit, human 

Human Vascular Endothelial Cell Growth Factor A 

ELISA 


ELISA 

Human Vascular Endothelial Cell Growth Factor C 

ELISA 

Human soluble Vascular Endothelial Growth Factor 

Receptor 1 ELISA 

Human Vascular Endothelial Cell Growth Factor 

Receptor 2 ELISA 




96 tests PK-EL-64407L 




VEGF-R III ELISA Kit, human 96 tests PK-EL-64428 

Mouse 

6-Ckine ELISA Kit, mouse Mouse 6-Ckine ELISA 96 tests PK-EL-66205M 

AXL ELISA Kit, mouse Mouse AXL ELISA 96 tests PK-EL-60080M 

BLC ELISA Kit, mouse Mouse B-Lymphocyte Chemoattractant ELISA 96 tests PK-EL-65240M 

CD30L ELISA Kit, mouse Mouse CD30 Ligand ELISA 96 tests PK-EL-66596M 

CD40L ELISA Kit, mouse Mouse CD40 Ligand ELISA 96 tests PK-EL-63106M 

CRG-2 ELISA Kit, mouse Mouse CRG-2 (Cytokine Responsive Genes 2) ELISA 96 tests PK-EL-66598M 

CTACK ELISA Kit, mouse 

Mouse Cuteaneous T-Cell Attracting Chemokine 

(CCL27) ELISA 

96 tests PK-EL-66590M 

CXCL16 ELISA Kit, mouse Mouse CXC Ligand 16 ELISA 96 tests PK-EL-66610M 

Eotaxin ELISA Kit, mouse Mouse Eotaxin ELISA 96 tests PK-EL-65320M 

G-CSF ELISA Kit, mouse Mouse Granulocyte Colony Stimulating Factor ELISA 96 tests PK-EL-60434M 

GM-CSF ELISA Kit, mouse 

Mouse Granulocyte/Macrophage Colony 

Stimulating Factor ELISA 


IFN-gamma ELISA Kit, mouse Mouse Interferon gamma ELISA 96 tests PK-EL-60706M 

IFN-gamma ELISA Kit, mouse* Mouse Interferon gamma ELISA 96 tests PK-EL-60707ML 

IGF-BP6 ELISA Kit, mouse Mouse Insulin Like Growth Factor Binding Protein 6 

ELISA 


IL-1 alpha ELISA Kit, mouse Mouse Interleukin 1-alpha ELISA 96 tests PK-EL-61106M 

IL-1 beta ELISA Kit, mouse Mouse Interleukin 1-beta ELISA 96 tests PK-EL-61126M 


ELISAs 



98 



IL-1 beta ELISA Kit, mouse* Mouse Interleukin 1 beta ELISA 96 tests PK-EL-61127ML 

IL-2 ELISA Kit, mouse Mouse Interleukin 2 ELISA 96 tests PK-EL-61206M 




IL-6 ELISA Kit, mouse* Mouse Interleukin 6 ELISA 96 tests PK-EL-61607ML 




IL-12 (p40 + p70) ELISA Kit, mouse Mouse Interleukin 12 ELISA 96 tests PK-EL-62206M 

IL-12 (p70) ELISA Kit, mouse Mouse Interleukin 12 (p70) ELISA 96 tests PK-EL-62216M 






IP-10 ELISA Kit, mouse Mouse IFN Inducible Protein 10 (CXCL10) ELISA 96 tests PK-EL-65520M 

Leptin ELISA Kit, mouse Mouse Leptin ELISA 96 tests PK-EL-68232M 

MCP-1 ELISA Kit, mouse Mouse Monocyte Chemotactic Protein 1 (CCL2) ELISA 96 tests PK-EL-64006M 


ELISAs 

MCP-5 ELISA Kit, mouse 

MIP-1 alpha ELISA Kit, mouse 

MIP-2 ELISA Kit, mouse 

Mouse Monocyte Chemotactic Protein 5 (CCL12) 

ELISA 

Mouse Macrophage Inflammatory Protein 1-alpha 

(CCL3) ELISA 

Mouse Macrophage Inflammatory Protein 2 (CXCL2) 

ELISA 




sP-selectin / CD62P ELISA Kit, mouse Mouse soluble P-selectin (CD62P) ELISA 96 tests PK-EL-69220M 

SCF ELISA Kit, mouse Mouse Stem Cell Factor ELISA 96 tests PK-EL-63120M 

SDF-1 alpha ELISA Kit, mouse Mouse Stromal Cell Derived Factor 1-alpha ELISA 96 tests PK-EL-67220M 

TECK ELISA Kit, mouse Mouse Thymus-expressed Chemokine (CCL25) ELISA 96 tests PK-EL-68020M 

TGF-beta 1 ELISA Kit, mouse Mouse Transforming Growth Factor beta-1 ELISA 96 tests PK-EL-63506M 

TIMP-1 ELISA Kit, mouse Mouse Tissue Inhibitor of Matrix Metalloprotease 1 

ELISA 

TIMP-2 ELISA Kit, mouse Mouse Tissue Inhibitor of Matrix Metalloprotease 2 

ELISA 



TNF-alpha ELISA Kit, mouse Mouse Tumor Necrosis Factor alpha ELISA 96 tests PK-EL-63706M 

TPO ELISA Kit, mouse Mouse Thrombopoietin ELISA 96 tests PK-EL-65112M 

sVCAM-1 / CD106 ELISA Kit, mouse Mouse soluble Vascular Cellular Adhesion Molecule 1 

(CD106) ELISA 

VEGF-A ELISA Kit, mouse 

VEGF-A ELISA Kit, mouse* 

Mouse Vascular Endothelial Cell Growth Factor A 

ELISA 


ELISA 



96 tests PK-EL-64407ML 





Rat 

Fractalkine ELISA Kit, rat Rat Fractalkine ELISA 96 tests PK-EL-67510R 

Fractalkine ELISA Kit, rat* Rat Fractalkine ELISA 96 tests PK-EL-67510RL 

GM-CSF ELISA Kit, rat 

Rat Granulocyte/Macrophage Colony Stimulating 

Factor ELISA 

96 tests PK-EL-60406R 

IFN-gamma ELISA Kit, rat Rat Interferon gamma ELISA 96 tests PK-EL-60706R 

IL-1 alpha ELISA Kit, rat Rat Interleukin 1-alpha ELISA 96 tests PK-EL-61106R 

IL-1 beta ELISA Kit, rat Rat Interleukin 1-beta ELISA 96 tests PK-EL-61126R 

IL-2 ELISA Kit, rat Rat Interleukin 2 ELISA 96 tests PK-EL-61206R 




LIX ELISA Kit, rat 

LIX ELISA Kit, rat* 

MCP-1 ELISA Kit, rat 

MCP-1 ELISA Kit, rat* 

Rat Lipopolysaccharide Induced CXC Chemokine 

(CXCL5) ELISA 

Rat Lipopolysaccharide Induced CXC Chemokine 

(CXCL5) ELISA 

Rat Monocyte Chemotactic Protein 1 (MCAF, CCL2) 

ELISA 

Rat Monocyte Chemotactic Protein 1 (MCAF, CCL2) 

ELISA 


96 tests PK-EL-66519RL 


96 tests PK-EL-64006RL 

TGF-beta 1 ELISA Kit, rat Rat Transforming Growth Factor beta-1 ELISA 96 tests PK-EL-63506R 

TIMP-1 ELISA Kit, rat Rat Tissue Inhibitor of Matrix Metalloprotease 1 ELISA 96 tests PK-EL-68559R 

TIMP-1 ELISA Kit, rat* Rat Tissue Inhibitor of Matrix Metalloprotease 1 ELISA 96 tests PK-EL-68559RL 

TNF-alpha ELISA Kit, rat Rat Tumor Necrosis Factor alpha ELISA 96 tests PK-EL-63706R 

VEGF-A ELISA Kit, rat Rat Vascular Endothelial Cell Growth Factor A ELISA 96 tests PK-EL-64407R 

VEGF-A ELISA Kit, rat* Rat Vascular Endothelial Cell Growth Factor A ELISA 96 tests PK-EL-64407RL 

VEGF-C ELISA Kit, rat Rat Vascular Endothelial Cell Growth Factor C ELISA 96 tests PK-EL-64416R 

Monkey 

sE-selectin / CD62E ELISA Kit, monkey Monkey soluble E-selectin (CD62E) ELISA 96 tests PK-EL-69216MK 

sICAM-1 / CD54 ELISA Kit, monkey Monkey soluble Intercellular Adhesion Molecule 1 

(CD54) ELISA 

96 tests PK-EL-63356MK 

IL-1 beta ELISA Kit, monkey Monkey Interleukin 1-beta ELISA 96 tests PK-EL-61126MK 

IL-2 ELISA Kit, monkey Monkey Interleukin 2 ELISA 96 tests PK-EL-61206MK 




IL-12 (p70) ELISA Kit, monkey Monkey Interleukin 12 (p70) ELISA 96 tests PK-EL-62216MK 

sPECAM-1 / CD31 ELISA Kit, monkey 

Monkey soluble Platelet Endothelial Cell Adhesion 



TNF-alpha (total) ELISA Kit, monkey Monkey Tumor Necrosis Factor alpha (total) ELISA 96 tests PK-EL-63708MK 

TNF-R I ELISA Kit, monkey 

Monkey soluble Tumor Necrosis Factor Receptor I 

ELISA 



ELISAs 



100 



Pig 

IFN-gamma ELISA Kit, pig Pig Interferon gamma ELISA 96 tests PK-EL-60706P 

IL-18 ELISA Kit, pig Pig Interleukin 18 ELISA 96 tests PK-EL-62816P 

n CD Marker 


Human 

sCD8 ELISA Kit, human Human soluble CD8 ELISA 96 tests PK-EL-63310 






ELISAs 

sPECAM-1 / CD31 ELISA Kit, human 

Human soluble Platelet Endothelial Cell Adhesion 




sCD44std ELISA Kit, human Human soluble CD44std ELISA 96 tests PK-EL-63349 

sCD44var (v5) ELISA Kit, human Human soluble CD44var (v5) ELISA 96 tests PK-EL-63350-5 

sCD44var (v6) ELISA Kit, human Human soluble CD44var (v6) ELISA 96 tests PK-EL-63350-6 


(CD50) ELISA 


(CD54) ELISA 



sE-selectin / CD62E ELISA Kit, human Human soluble E-selectin (CD62E) ELISA 96 tests PK-EL-69216 

sP-selectin / CD62P ELISA Kit, human Human soluble P-selectin (CD62P) ELISA 96 tests PK-EL-69220 


(CD102) ELISA 

sVCAM-1 / CD106 ELISA Kit, human Human soluble Vascular Cellular Adhesion Molecule 1 

(CD106) ELISA 



OX-40 / CD134 ELISA Kit, human Human soluble CD134 (OX-40) ELISA 96 tests PK-EL-68166 

4-1BB / CD137 ELISA Kit, human Human soluble 4-1BB (CD137) ELISA 96 tests PK-EL-66556 

sVE-Cadherin / CD144 ELISA Kit, 

human 

Human soluble VE-Cadherin (CD144) ELISA 96 tests PK-EL-60266 

sCTLA-4 / CD152 ELISA Kit, human Human soluble CTLA-4 (CD152) ELISA 96 tests PK-EL-63396 

sCD153 / sCD30L ELISA Kit, human Human soluble CD153 (sCD30L) ELISA 96 tests PK-EL-66596 

sPSGL-1 / CD162 ELISA Kit, human 

Human soluble CD162 (soluble P-selectin Glycoprotein 

Ligand 1) ELISA 


Mouse 

sP-selectin / CD62P ELISA Kit, mouse Mouse soluble P-selectin (CD62P) ELISA 96 tests PK-EL-69220M 

sVCAM-1 / CD106 ELISA Kit, mouse Mouse Vascular Cellular Adhesion Molecule 1 (CD106) 

ELISA 





Monkey 

sPECAM-1 / CD31 ELISA Kit, monkey 

Monkey soluble Platelet Endothelial Cell Adhesion 


sICAM-1 / CD54 ELISA Kit, monkey Monkey soluble Intercellular Adhesion Molecule 1 

(CD54) ELISA 



sE-selectin / CD62E ELISA Kit, monkey Monkey soluble E-selectin (CD62E) ELISA 96 tests PK-EL-69216MK 


monkey 

Monkey soluble VE-Cadherin (CD144) ELISA 96 tests PK-EL-60266MK 

n Hormones 


Relaxin ELISA Kit, human Human Relaxin ELISA 96 tests PK-EL-K9210 

Thymosin alpha-1 ELISA Kit, human Human Thymosin alpha-1 ELISA 96 tests PK-EL-K9510 

Thymosin beta-4 ELISA Kit, human Human Thymosin beta-4 ELISA 96 tests PK-EL-K9520 

Thymulin ELISA Kit, human Human Thymulin ELISA 96 tests PK-EL-K9810 

n Apoptosis 


Human 

Annexin V ELISA Kit, human Human Annexin V ELISA 96 tests PK-EL-69006 

anti-Annexin V ELISA Kit, human Human anti-Annexin V ELISA 96 tests PK-EL-69036 

APO-1 (Fas) ELISA Kit, human Human soluble Apoptosis Antigen 1 (Fas) ELISA 96 tests PK-EL-69206 

APRIL ELISA Kit, human Human APRIL ELISA 96 tests PK-EL-69046 

BAFF ELISA Kit, human Human B-cell Activating Factor (BLyS) ELISA 96 tests PK-EL-K9410 

Bcl-2 ELISA Kit, human Human B-cell Lymphoma/Leukemia 2 ELISA 96 tests PK-EL-69056 

Caspase-8 / FLICE ELISA Kit, human Human Caspase 8 (FLICE) ELISA 96 tests PK-EL-69076 

Caspase-9 ELISA Kit, human Human Caspase 9 ELISA 96 tests PK-EL-69086 

Cathepsin K ELISA Kit, human Human Cathepsin K ELISA 96 tests PK-EL-KB20432 

Cathepsin L ELISA Kit, human Human Cathepsin L ELISA 96 tests PK-EL-69066 

CD30 ELISA Kit, human Human soluble CD30 ELISA 96 tests PK-EL-63338 

CD40 ELISA Kit, human Human soluble CD40 ELISA 96 tests PK-EL-63105 

CD40L ELISA Kit, human Human soluble CD40 Ligand ELISA 96 tests PK-EL-63106 

Cu/ZnSOD ELISA Kit, human Human Copper/Zinc Superoxide Dismutase ELISA 96 tests PK-EL-69096 

Cytochrome c ELISA Kit, human Human Cytochrome c ELISA 96 tests PK-EL-69106 

DcR3 ELISA Kit, human Human Decoy Receptor 3 ELISA 96 tests PK-EL-69116 

Granzyme A ELISA Kit, human Human Granzyme A ELISA 96 tests PK-EL-69146 


ELISAs 


102 



Granzyme B ELISA Kit, human Human Granzyme B ELISA 96 tests PK-EL-69156 

OPG ELISA Kit, human Human Osteoprotegerin (OPG) ELISA 96 tests PK-EL-63750 

OX-40 / CD134 ELISA Kit, human Human CD134 (OX-40) ELISA 96 tests PK-EL-68166 

sFas Ligand ELISA Kit, human Human soluble Fas Ligand ELISA 96 tests PK-EL-69226 


human 

Human soluble VE-Cadherin (CD144) ELISA 96 tests PK-EL-60266 

TRAIL ELISA Kit, human Human TNF-Related Apoptosis Inducing Ligand ELISA 96 tests PK-EL-63606 

Monkey 


monkey 

Monkey soluble VE-Cadherin (CD144) ELISA 96 tests PK-EL-60266MK 

n Other ELISAs 


1,25-Dihydroxy Vitamin D ELISA Kit, 

human 

α1-Antitrypsin (α1-Proteinase Inhibitor) 

ELISA Kit, human 

α1-Microglobulin (α1-M) ELISA Kit, 

human 

Human 1,25-(OH) 2 

Vitamin D ELISA 48 tests PK-EL-K2112 

Human a1-Antitrypsin (α1-Proteinase Inhibitor) ELISA 96 tests PK-EL-K6750 

Human a1-Mikroglobulin (α1-M) ELISA 96 tests PK-EL-K6710 

α2-Macroglobulin ELISA Kit, human Human a2-Macroglobulin (α2-M) ELISA 96 tests PK-EL-K6610 

ADMA ELISA Kit, human Human Asymetric Dimethyl Arginine ELISA 96 tests PK-EL-K7812 

β2-Microglobulin (β2-M) ELISA Kit, 

human 

Human β2-Microglobulin (β2-M) ELISA 96 tests PK-EL-68240 

Big Endothelin ELISA Kit, human Human Big Endothelin ELISA 96 tests PK-EL-KB20082 

BSP ELISA Kit, human Human Bone Sialoprotein (BSP) ELISA 96 tests PK-EL-K4220 

Calprotectin (MRP 8/14) ELISA Kit, 

human 

Human Calprotectin (MRP 8/14) ELISA 96 tests PK-EL-K6935 

Cathepsin K ELISA Kit, human Human Cathepsin K ELISA 96 tests PK-EL-KB20432 


ELISAs 

DKK-1 ELISA Kit, human Human Dickkopf-1 (DKK-1) ELISA 96 tests PK-EL-KB20412 

EDN (EPX) ELISA Kit, human Human Eosinophil Derived Neurotoxin (EPX) ELISA 96 tests PK-EL-K6810 

Endothelin (1-21) ELISA Kit, human Human Endothelin (1-21) ELISA 96 tests PK-EL-KB1140 

Fibronectin ELISA Kit, human Human Fibronectin ELISA 96 tests PK-EL-69126 

Galectin-3 ELISA Kit, human Human Galectin 3 (Mac-2) ELISA 96 tests PK-EL-69136 

Immunglobulin E [IgE] ELISA Kit, human Human Immunglobulin E [IgE] ELISA 96 tests PK-EL-K6511 

Immunglobulin G [IgG] ELISA Kit, 

human 

Human Immunglobulin G [IgG] ELISA 96 tests PK-EL-K6510 

NT-proANP 1-98 ELISA Kit, human Human Atrial Natriuretic proPeptid (proANP 1-98) 

ELISA 

NT-proCNP ELISA Kit, human 

Human C-Type Natriuretic Propeptid (NT-proCNP) 

ELISA 



Osteonectin (ON) ELISA Kit, human Human Osteonectin (ON) ELISA 96 tests PK-EL-K4230 

Oxidized LDL (oxLDL) ELISA Kit, human Human Oxidized LDL (oxLDL) ELISA 96 tests PK-EL-K7810 




p53 ELISA Kit, human Human p53 ELISA 96 tests PK-EL-69176 

RBP ELISA Kit, human Human Retinol Binding Protein (RBP) ELISA 96 tests PK-EL-K6110 

Thyreoglobulin (hTG) ELISA Kit, human Human Thyreoglobulin (hTG) ELISA 96 tests PK-EL-K7510 

Thyreoglobulin (hTG, recovery) ELISA 

Kit, human 

Human Thyreoglobulin (hTG, recovery) ELISA 96 tests PK-EL-K7510A 

Urotensin II ELISA Kit, human Human Urotensin II ELISA 96 tests PK-EL-K1013 

VDBP ELISA Kit, human Human Vitamin D Binding Protein ELISA 96 tests PK-EL-K2314 

Vitamin D (direct) ELISA Kit, human Human Vitamin D (direct) ELISA 96 tests PK-EL-K2109 

Zonulin ELISA Kit, human Human Zonulin ELISA 96 tests PK-EL-K5600 

Mouse/Rat 

ADMA direct ELISA Kit, mouse/rat 

Mouse/Rat ADMA direct 

(Asymetric Dimethyl Arginine) ELISA 

96 tests PK-EL-K3001 

n ELISA Development Kits 


Human 

4-1BB Receptor (CD137) EDK, human 

Human 4-1BB Receptor (CD137) 

ELISA Development Kit 

1000 tests PK-EL-66551D 

BD-1 EDK, human Human beta-Defensin 1 ELISA Development Kit 1000 tests PK-EL-65251D 



Betacellulin EDK, human Human Betacellulin ELISA Development Kit 1000 tests PK-EL-67360D 

BMP-2 EDK, human Human Bone Morphogenetic Protein 2 



sCD40L EDK, human Human sCD40 Ligand ELISA Development Kit 1000 tests PK-EL-63100D 

CTGF EDK, human 

CNTF EDK, human 

Human Connective Tissue Growth Factor 


Human Ciliary Neurotrophic Factor 




CXCL16 EDK, human Human CXC Ligand 16 ELISA Development Kit 1000 tests PK-EL-66610D 

EGF EDK, human 

Human Epidermal Growth Factor 



Eotaxin (CCL11) EDK, human Human Eotaxin (CCL11) ELISA Development Kit 1000 tests PK-EL-65320D 

Eotaxin-3 EDK, human Human Eotaxin 3 ELISA Development Kit 1000 tests PK-EL-65340D 

FGF-2 (FGF-b) EDK, human 

Human Fibroblast Growth Factor basic (FGF-2) 



Follistatin EDK, human Human Follistatin ELISA Development Kit 1000 tests PK-EL-67720D 

G-CSF EDK, human 

GM-CSF EDK, human 

Human Granulocyte Colony Stimulating Factor 


Human Granulocyte/Macrophage Colony Stimulating 

Factor ELISA Development Kit 




ELISAs 


104 




ELISAs 

GRO-alpha (MGSA, CXCL1) EDK, 

human 

GRO-beta (MIP-2, CXCL2) EDK, human 

Human Growth Regulated Oncogen alpha 

(MGSA, CXCL1) ELISA Development Kit 

Human Growth Regulated Oncogen beta 

(MIP-2, CXCL2) ELISA Development Kit 



IFN-gamma EDK, human Human Interferon gamma ELISA Development Kit 1000 tests PK-EL-60725D 

IL-1 alpha EDK, human Human Interleukin 1-alpha ELISA Development Kit 1000 tests PK-EL-61112D 

IL-2 EDK, human Human Interleukin 2 ELISA Development Kit 1000 tests PK-EL-61240D 











IL-17A EDK, human Human Interleukin 17A ELISA Development Kit 1000 tests PK-EL-62710D 

IL-17E EDK, human Human Interleukin 17E ELISA Development Kit 1000 tests PK-EL-62715D 

IL-17F EDK, human Human Interleukin 17F ELISA Development Kit 1000 tests PK-EL-62718D 






IP-10 EDK, human Human IFN Inducible Protein 10 

(CXCL10) ELISA Development Kit 

I-TAC EDK, human 

Human IFN Inducible T-cell alpha Chemoattractant 

(CXCL11) ELISA Development Kit 



Leptin EDK, human Human Leptin ELISA Development Kit 1000 tests PK-EL-68232D 

MCP-1 (MCAF, CCL2) EDK, human Human Monocyte Chemotactic Protein 1 

(MCAF, CCL2) ELISA Development Kit 

MIA-2 EDK, human Human Melanoma Inhibitory Activity Protein 2 




Midkine (MK) EDK, human Human Midkine (MK) ELISA Development Kit 1000 tests PK-EL-67950D 

MIP-1 alpha (CCL3) EDK, human 

NAP-2 (CXCL7) EDK, human 

beta-NGF EDK, human 


(CCL3) ELISA Development Kit 

Human Neutrophil Activating Protein 2 (CXCL7) 


Human Nerve Growth Factor beta 





NOV EDK, human Human NOV ELISA Development Kit 1000 tests PK-EL-64335D 

PAI-1 EDK, human Human Plasminogen Activator Inhibitor 1 






PDGF-BB EDK, human 

Human Platelet Derived Growth Factor BB 


PLGF-1 EDK, human Human Placenta Growth Factor 1 




sRANKL EDK, human Human sRANK Ligand ELISA Development Kit 1000 tests PK-EL-63200D 

RANTES (CCL5) EDK, human Human RANTES (CCL5) ELISA Development Kit 1000 tests PK-EL-64130D 

Resistin EDK, human Human Resistin ELISA Development Kit 1000 tests PK-EL-66670D 

SCF EDK, human Human Stem Cell Factor ELISA Development Kit 1000 tests PK-EL-63120D 

TACI EDK, human Human TACI ELISA Development Kit 1000 tests PK-EL-66690D 

TL-1A EDK, human Human TL-1A ELISA Development Kit 1000 tests PK-EL-66695D 

TNF-alpha EDK, human 

TPO (MDGF) EDK, human 

Human Tumor Necrosis Factor alpha 


Human Thrombopoietin (MDGF) 




sTRAIL (APO2L) EDK, human Human sTRAIL (APO2L) ELISA Development Kit 1000 tests PK-EL-63600D 

TSLP EDK, human 

Human Thymic Stromal Lymphopoietin 



TWEAK EDK, human Human TWEAK ELISA Development Kit 1000 tests PK-EL-63610D 

VEGF EDK, human 

Mouse 

EGF EDK, mouse 

G-CSF EDK, mouse 

GM-CSF EDK, mouse 



Mouse Epidermal Growth Factor 


Mouse Granulocyte Colony Stimulating Factor 


Mouse Granulocyte/Macrophage Colony Stimulating 

Factor ELISA Development Kit 


1000 tests PK-EL-60170DM 



IFN-gamma EDK, mouse Mouse Interferon gamma ELISA Development Kit 1000 tests PK-EL-60725DM 

IGF-I EDK, mouse 

Mouse Insulin Like Growth Factor I 



IL-1 alpha EDK, mouse Mouse Interleukin 1-alpha ELISA Development Kit 1000 tests PK-EL-61112DM 

IL-1 beta EDK, mouse Mouse Interleukin 1-beta ELISA Development Kit 1000 tests PK-EL-61120DM 

IL-2 EDK, mouse Mouse Interleukin 2 ELISA Development Kit 1000 tests PK-EL-61240DM 







KC (GRO-alpha, CXCL1) EDK, mouse 

Mouse Keratinocyte Chemoattractant 

(GRO-alpha, CXCL1) ELISA Development Kit 


Leptin EDK, mouse Mouse Leptin ELISA Development Kit 1000 tests PK-EL-68232DM 

MCP-1 (JE, CCL2) EDK, mouse 

Mouse Monocyte Chemotactic Protein 1 (JE, CCL2) 




ELISAs 


106 



MCP-3 (CCL7) EDK, mouse 

M-CSF EDK, mouse 

MDC (CCL22) EDK, mouse 

MIP-1 alpha (CCL3) EDK, mouse 

MIP-2 (GRO-beta, CXCL2) EDK, mouse 

Mouse Monocyte Chemotactic Protein 3 (CCL7) ELISA 

Development Kit 

Mouse Macrophage Colony Stimulating Factor ELISA 


Mouse Macrophage Derived Chemokine (CCL22) 


Mouse Macrophage Inflammatory Protein 1-alpha 

(CCL3) ELISA Development Kit 

Mouse Macrophage Inflammatory Protein 2 (GRObeta, 

CXCL2) ELISA Development Kit 






sRANKL EDK, mouse Mouse sRANK Ligand ELISA Development Kit 1000 tests PK-EL-63200DM 

RANTES (CCL5) EDK, mouse Mouse RANTES (CCL5) ELISA Development Kit 1000 tests PK-EL-64130DM 

SCF EDK, mouse Mouse Stem Cell Factor ELISA Development Kit 1000 tests PK-EL-63120DM 

TNF-alpha EDK, mouse 

VEGF EDK, mouse 

Rat 

GRO (KC, CXCL1) EDK, rat 

Mouse Tumor Necrosis Factor alpha 




Rat Growth Regulated Oncogen (KC, CXCL1) ELISA 




1000 tests PK-EL-65240DR 

IFN-gamma EDK, rat Rat Interferon gamma ELISA Development Kit 1000 tests PK-EL-60725DR 

IL-1 beta EDK, rat Rat Interleukin 1-alpha ELISA Development Kit 1000 tests PK-EL-61112DR 

IL-1 alpha EDK, rat Rat Interleukin 1-beta ELISA Development Kit 1000 tests PK-EL-61120DR 

IL-6 EDK, rat Rat Interleukin 6 ELISA Development Kit 1000 tests PK-EL-61620DR 

MCP-1 (CCL2) EDK, rat 

MIP-1 alpha (CCL4) EDK, rat 

Rat Monocyte Chemotactic Protein 1 (CCL2) ELISA 


Rat Macrophage Inflammatory Protein 1-alpha (CCL4) 




RANTES (CCL5) EDK, rat Rat RANTES (CCL5) ELISA Development Kit 1000 tests PK-EL-64130DR 

SCF EDK, rat Rat Stem Cell Factor ELISA Development Kit 1000 tests PK-EL-63120DR 

TNF-alpha EDK, rat Rat Tumor Necrosis Factor alpha ELISA Development Kit 1000 tests PK-EL-63720DR 


ELISAs 


6.4 Antigens and Cell Lysates 

The PromoKine peptides are perfectly 

suited as blocking reagent in combination 

with the PromoKine antibodies and 

as a control in e.g. immunoblotting/ 

Western blot, ELISA, or immunohistochemistry 

assays. 

PromoKine also provides a wide range 

of ready-to-use cell and tissue lysates 

that can be used as controls in e.g. gel 

electrophoresis, Western blotting, or immunoprecipitation. 

Moreover, they are 

also useful in enzymatic activity analysis, 

protein-protein interaction analysis, and 

tissue specific expression identification. 

The total tissue and cell line protein 

lysates are available from human, mouse, 

and rat origin as well as from a variety 

of developmental stages. Furthermore, 

beside lysates from normal tissue also 

lysates from tumor tissue and diseased 

tissue are available. 

For a complete list of our peptides and cell lysates please see 

www.promokine.info/antigens-and-cell-lysates or the PromoKine Price List.

Cytokines and Growth 

7 

Factors 

Cytokines and chemokines are small proteins secreted by a 

variety of cell types. They are important signal messengers 

mediating cell communication and activating other target cells 

through binding to specific receptors. Cytokines play a central 

role in the immune system - in which the chemokines mainly 

function as chemoattractants. They also coordinate and regulate 

other biological processes such as cell growth and development 

or tissue repair.

108 


7 

Cytokines and Growth 

Factors 

7.1 Cytokines, Chemokines, Growth Factors, 

Hormones, and soluble Receptors ...... 109 

7.2 Animal-free Cytokines and 

Growth Factors .................... 131 

7.3 CD Antigens. ..................... 134 

7.4 Cytokines and Growth Factors 

for Stem Cell Research. .............. 135

7 Cytokines and Growth Factors 109 

7.1 Cytokines, Chemokines, Growth Factors, Hormones, and soluble Receptors 

All cytokines, chemokines, growth factors, 

CD antigens, hormones, and soluble 

receptors available from PromoKine 

meet the highest quality standards. They 

are highly purified and biologically active. 

Purity is generally assessed by SDS-PAGE 

and HPLC analysis and is in most cases 

higher than 95%. Most of the products 

are delivered lyophilized at room temperature 

and some come ready-to-use in 

a buffer solution. Reconstitution of the 

lyophilized proteins should be carried out 

in water or common buffer solutions. 

We continuously add new recombinant 

proteins to our inventory. 

For an up-to-date product list please 

visit www.cytokines.info 

n Human 

Product Product Description Size* Catalog Number 

4-1BB sReceptor (CD137), human, 

recombinant 

Recombinant Human 4-1BB soluble Receptor (CD137) 20 µg C-66551 

4-1BBL (CD137L), human, recombinant Recombinant Human 4-1BB Ligand (CD137L) 20 µg C-66550 

Acrp30 (His), human, recombinant 

Acrp30, human, recombinant (E. coli) 

Acrp30, human, recombinant (HEK) 

Acrp30, human, recombinant (InC) 

Recombinant Human Adiponectin (Adipocyte Complement 

Related Protein of 30 kDa; E. coli-derived, His-tagged) 


Related Protein of 30 kDa; E. coli-derived) 


Related Protein of 30 kDa; HEK cell-derived) 


Related Protein of 30 kDa; Hi-5 insect cell-derived) 

50 µg C-66625 

25 µg C-66626 

10 µg C-66628 

25 µg C-66629 

gAcrp30 (Adipolean), human, 

recombinant 

gAcrp30/Adipolean Variant, human, 

recombinant 

Recombinant Human globular Acrp30 (Adipolean) 25 µg C-66630 

Recombinant Human globular Acrp30/Adipolean Variant 25 µg C-66631 

Activin A, human, recombinant Recombinant Human Activin A 5 µg C-60060 

Activin B, human, recombinant Recombinant Human Activin B 5 µg C-60062 

AITRL, human, recombinant 

Recombinant Human Activation Induced TNFR Member 

Ligand 

20 µg C-63735 

Ang-1, human, recombinant (HeLa) Recombinant Human Angiopoietin 1 (HeLa cell-derived) 20 µg C-60014 

Ang-1, human, recombinant (Sf9) Recombinant Human Angiopoietin 1 

(Sf9 insect cell-derived) 

Ang-2, human, recombinant (Sf9) Recombinant Human Angiopoietin 2 


10 µg C-60015 

10 µg C-60020 

Angiogenin-1, human, recombinant 

(Plant) 

Recombinant Human Angiogenin-1 (plant-derived) 50 µg C-60110A 

Angiostatin K1-3, human, recombinant Recombinant Human Angiostatin Kringles 1-3 100 µg C-60090 

Angiostatin K1-4, human Human Angiostatin Kringles 1-4 10 µg C-60095 

ANGPTL-3, human, recombinant Recombinant Human Angiopoietin-like Protein 3 10 µg C-60070 

ANGPTL-4, human, recombinant Recombinant Human Angiopoietin-like Protein 4 10 µg C-60072 

APO-A1, human, recombinant Recombinant Human Apolipoprotein A1 100 µg C-60016 

APO-E2, human, recombinant Recombinant Human Apolipoprotein E2 500 µg C-60017 



7 Cytokines and 

Growth Factors 

Apolipoprotein-D, human, recombinant Recombinant Human Apolipoprotein D 10 µg C-68245 

Apolipoprotein-J, human, recombinant Recombinant Human Apolipoprotein J (ApoJ, Clusterin) 10 µg C-68250 

Apo-SAA, human, recombinant Recombinant Human Apolipoprotein Serum Amyloid A 50 µg C-68302 

Apo-SAA1, human, recombinant Recombinant Human Apolipoprotein Serum Amyloid A1 50 µg C-68303 


* Economic bulk sizes are also available! Please request!

110 

7 Cytokines and Growth Factors 




APRIL, human, recombinant 

Recombinant Human APRIL (A Proliferation Inducing 

Ligand, TALL-2, TRDL-1a, TNFSF13) 

10 µg C-63745 

Artemin, human, recombinant Recombinant Human Artemin 10 µg C-60030 

BAFF, human, recombinant 

BAFF-R, human, recombinant 

BCA-1 (BLC, CXCL13), human, 

recombinant 

Recombinant Human B-cell Activating Factor (BLyS) 

belonging to the TNF Family 

Recombinant Human B-cell Activating Factor (BLyS) 

Receptor belonging to the TNF Family 

Recombinant Human B-cell Attracting Chemokine 1 

(BLC, CXCL13) 

20 µg C-63755 

20 µg C-63758 

20 µg C-65240 

BCMA, human, recombinant Recombinant Human B-cell Maturation Protein 20 µg C-66570 

BD-1 (36 aa), human, recombinant Recombinant Human beta-Defensin 1 (36 amino acid variant) 20 µg C-65251 

BD-1 (47 aa), human, recombinant Recombinant Human beta-Defensin 1 (47 amino acid variant) 20 µg C-65254 

BD-2, human, recombinant Recombinant Human beta-Defensin 2 20 µg C-65250 



BDNF, human, recombinant Recombinant Human Brain Derived Neurotrophic Factor 10 µg C-66212 

BMP-2, human, recombinant Recombinant Human Bone Morphogenetic Protein 2 10 µg C-67310 





BMP-7, human, recombinant (CHO) Recombinant Human Bone Morphogenetic Protein 7 

(CHO cell-derived) 

BMP-7, human, recombinant (E. coli) Recombinant Human Bone Morphogenetic Protein 7 

(E. coli-derived) 

BMP-13 (CDMP-2), human, 

recombinant 

BMP-14 (CDMP-1), human, 

recombinant 

sBMPR-1A, human, recombinant (Sf9) 

Recombinant Human Bone Morphogenetic Protein 13 

(CDMP-2) 

Recombinant Human Bone Morphogenetic Protein 14 

(CDMP-1) 

Recombinant Human soluble Bone Morphogenetic 

Protein Receptor 1A (Sf9 insect cell-derived) 

10 µg C-67319 

10 µg C-67318 

50 µg C-67340 

50 µg C-67344 

100 µg C-67345 

BNP, human, recombinant Recombinant Human B-type Natriuretic Protein 10 µg C-60065 

BRAK (CXCL14), human, recombinant 

Recombinant Human Human Breast and Kidney 

Chemokine (CXCL14) 

20 µg C-66580 

Betacellulin (BTC), human, recombinant Recombinant Human Betacellulin (BTC) 20 µg C-60510 

Cardiotrophin-1 (CT-1), human, 

recombinant 

Recombinant Human Cardiotrophin-1 (CT-1) 10 µg C-67410 

CD1A, human, recombinant Recombinant Human CD1A 2 µg C-63300 

CD2, human, recombinant Recombinant Human CD2 2 µg C-63303 

sCD4, human, recombinant Recombinant Human soluble CD4 10 µg C-60056 

CD4 (125-202 aa), human, recombinant Recombinant Human CD4 (125-202 amino acid variant) 20 µg C-60083 



CD11b, human, recombinant Recombinant Human CD11b 2 µg C-63318 





CD14, human, recombinant (CHO) Recombinant Human CD14 (CHO cell-derived) 2 µg C-63324 




CD31 (PECAM-1), human, recombinant Recombinant Human CD31 (PECAM-1) 2 µg C-63339 


sCD40 Ligand (CD154), human, 

recombinant 

Recombinant Human soluble CD40 Ligand (TRAP, 

CD154) 

50 µg C-63100 



CD54 (ICAM-1), human, recombinant Recombinant Human Intercellular CD54 (ICAM-1) 50 µg C-63356 






sCD105 (Endoglin), human, 

recombinant (E. coli) 


recombinant (Sf9) 

Recombinant Human soluble CD105 

(Endoglin, E. coli-derived) 


(Endoglin; Sf9 insect cell-derived) 

5 µg C-60059 

25 µg C-60058 



CD152 (CTLA-4), human, recombinant 

Recombinant Human CD152 

(Cytotoxic T-lymphocyte-Associated Antigen 4) 

50 µg C-63396 

CNTF, human, recombinant Recombinant Human Ciliary Neurotrophic Factor 20 µg C-66210 

CRP, human, recombinant Recombinant Human C-Reactive Protein 500 µg C-66200 

CTACK (CCL27), human, recombinant 

CTGF (182-250 aa, His), human, 

recombinant 

CTGF (His), human, recombinant 

CTGF (98 aa), human, recombinant 

CTGF, human, recombinant (HEK) 

CTGFL (WISP-2), human, recombinant 

Recombinant Human Cutaneous T-cell Attracting 

Chemokine (CCL27) 

Recombinant Human Connective Tissue Growth Factor 

(182-250 amino acid variant; His-tagged) 


(His-tagged) 


(98 amino acid variant) 


(HEK cell-derived) 


Like Protein (WISP-2) 

20 µg C-66590 

20 µg C-68016 

10 µg C-68015 

20 µg C-68014 

10 µg C-68017 

20 µg C-68018 

CXCL16, human, recombinant Recombinant Human CXC Ligand 16 25 µg C-66610 

CYR61, human, recombinant 

Recombinant Human Cysteine-rich Angiogenic 

Inducer 61 

20 µg C-63398 

DHH, human, recombinant Recombinant Human Desert Hedgehog 25 µg C-66682 

DKK-1, human, recombinant Recombinant Human Dickkopf-Related Protein-1 10 µg C-68320 

sDLL-1, human, recombinant Recombinant Human soluble Delta-like Protein 1 25 µg C-68037 





112 



sDLL-4, human, recombinant Recombinant Human soluble Delta-like Protein 4 25 µg C-68040 

EBI-3, human, recombinant Recombinant Human Epstein-Barr Virus Induced Gene 3 10 µg C-68112 



EGF, human, recombinant (E. coli) 

EGF, human, recombinant (Pichia) 

sEGF-R, human, recombinant 

EG-VEGF, human, recombinant 

EMAP-2, human, recombinant (Plant) 

EMAP-2, human, recombinant (E. coli) 

ENA-78 (74 aa), human, recombinant 

Recombinant Human Epidermal Growth Factor 


Recombinant Human Epidermal Growth Factor 

(Pichia-derived) 

Recombinant Human soluble Epidermal Growth Factor 

Receptor 

Recombinant Human Vascular Endothelial Cell Growth 

Factor-EG 

Recombinant Human Endothelial/Monocyte Activating 

Polypeptide 2 (plant-derived) 


Polypeptide 2 (E. coli-derived) 

Recombinant Human Epithelial Neutrophil Activating 

Peptide-78 (74 amino acid variant) 

500 µg C-60170 

500 µg C-60182 

25 µg C-60185 

20 µg C-64413 

10 µg C-65390A 

20 µg C-65390 

20 µg C-65230 

Endostatin, human, recombinant Recombinant Human Endostatin 100 µg C-68305 

Eotaxin (CCL11), human, recombinant Recombinant Human Eotaxin (CCL11) 20 µg C-65321 

Eotaxin-2 (CCL24), human, recombinant Recombinant Human Eotaxin 2 (CCL24) 20 µg C-65330 

Eotaxin-3 (CCL26), human, recombinant Recombinant Human Eotaxin 3 (CCL26) 20 µg C-65340 

Epigen, human, recombinant Recombinant Human Epigen (EPGN) 25 µg C-68380 

Epiregulin, human, recombinant Recombinant Human Epiregulin (EREG) 25 µg C-68382 

EPO-alpha, human, recombinant Recombinant Human Erythropoietin alpha 50 µg C-60022 

E-selectin (CD62E), human, recombinant Recombinant Human E-selectin (CD62E) 10 µg C-69217 

Exodus-2, human, recombinant Recombinant Human Exodus 2 20 µg C-66512 

sFas Ligand, human, recombinant Recombinant Human soluble Fas Ligand 10 µg C-66620 

sFas Receptor (CD95), human, 

recombinant 

FGF-1 (FGF-a), human, recombinant 

(E. coli) 


(Plant) 


(Sf9) 

FGF-2 (FGF-b), human, recombinant 

(E. coli) 


(Sf9) 


(Plant) 

FGF-2 (FGF-b, 147 aa), human, 

recombinant 

Recombinant Human soluble Fas Receptor 

(CD95, TNFRSF6) 

Recombinant Human acidic Fibroblast Growth Factor 1 

(FGF-a; E. coli-derived) 


(FGF-a; plant-derived) 


(FGF-a; Sf9 insect cell-derived) 

Recombinant Human basic Fibroblast Growth Factor 2 

(FGF-b; E. coli-derived)) 


(FGF-b; Sf9 insect cell-derived) 


(FGF-b; plant-derived) 


(FGF-b, 147 amino acid variant) 

20 µg C-66623 

50 µg C-60340 

50 µg C-60340A 

10 µg C-60343 

50 µg C-60240 

10 µg C-60245 

50 µg C-60240A 

50 µg C-60243 

FGF-4, human, recombinant Recombinant Human Fibroblast Growth Factor 4 25 µg C-60345 








FGF-9, human, recombinant (E. coli) Recombinant Human Fibroblast Growth Factor 9 


FGF-9, human, recombinant (Sf9) Recombinant Human Fibroblast Growth Factor 9 


20 µg C-60389 

20 µg C-60390 











sFGFR-1/Fc, human, recombinant (Sf9) 




flt-3 Ligand, human, recombinant 

(E. coli) 

Recombinant Human Fibroblast soluble Growth Factor 

Receptor 1a (IIIc) / Fc-Chimera (Sf9 insect cell-derived) 






Receptor 4 (IIIc) / Fc-Chimera (Sf9 insect cell-derived) 

10 µg C-60450 

10 µg C-60451 

10 µg C-60452 

10 µg C-60453 

Recombinant Human flt-3 Ligand (E. coli-derived) 10 µg C-67110 

flt-3 Ligand, human, recombinant (Plant) Recombinant Human flt-3 Ligand (plant-derived) 10 µg C-67110A 

flt-3 Ligand, human, recombinant (Sf9) Recombinant Human flt-3 Ligand (Sf9 insect cell-derived) 10 µg C-67113 

Fractalkine (CX3CL1), human, 

recombinant 

Recombinant Human Fractalkine (CX3CL1) 20 µg C-67510 

sFRP-1, human, recombinant Recombinant Human secreted Frizzled Related Protein 1 20 µg C-65270 

FST, human, recombinant Recombinant Human Follistatin 20 µg C-60040 

Galectin-1, human, recombinant Recombinant Human Galectin-1 50 µg C-67953 

Galectin-3, human, recombinant Recombinant Human Galectin-3 50 µg C-67958 

Galectin-4, human, recombinant Recombinant Human Galectin 4 25 µg C-67960 

GCP-2 (CXCL6), human, recombinant 

G-CSF, human, recombinant (CHO) 

G-CSF, human, recombinant (E. coli) 

G-CSF, human, recombinant (Plant) 

Recombinant Human Human Granulocyte Chemotactic 

Peptide 2 (CXCL6) 

Recombinant Human Granulocyte Colony Stimulating 

Factor (CHO cell-derived) 


Factor (E. coli-derived) 


Factor (plant-derived) 

20 µg C-67610 

10 µg C-60437 

10 µg C-60434 

10 µg C-60434A 

GDF-2, human, recombinant Recombinant Human Growth & Differentiation Factor 2 10 µg C-67322 






114 




GDF-8, human, recombinant Recombinant Human Growth & Differentiation Factor 8 

(Myostatin Propeptide) 

25 µg C-67333 


GDF-15 (MIC-1), human, recombinant Recombinant Human Growth & Differentiation Factor 15 

(MIC-1) 

20 µg C-67329 



GDNF, human, recombinant (E. coli) 

GDNF, human, recombinant (Plant) 

Recombinant Human Glial Derived Neurotropic Factor 



(plant-derived) 

10 µg C-66312 

10 µg C-66312A 

GH-20K-pit, human, recombinant Recombinant Human Pituitary Growth Hormone 20K 100 µg C-67336 

GH-20K-pl, human, recombinant Recombinant Human Placental Growth Hormone 20K 100 µg C-67337 

GH-22K-pl, human, recombinant Recombinant Human Placental Growth Hormone 22K 100 µg C-67339 

GHBP, human, recombinant Recombinant Human Growth Hormone Binding Protein 100 µg C-67338 

GM-CSF, human, recombinant (E. coli) 

GM-CSF, human, recombinant (Sf9) 

GM-CSF, human, recombinant (Plant) 

GM-CSF/IL-3 FP, human, recombinant 

(E. coli) 

Recombinant Human Granulocyte/Macrophage Colony 

Stimulating Factor (E. coli-derived) 


Stimulating Factor (Sf9 insect cell-derived) 


Stimulating Factor (plant-derived) 


Stimulating Factor/IL-3 Fusion Protein (E. coli-derived) 

10 µg C-60420 

20 µg C-60426 

10 µg C-60420A 

10 µg C-60428 

GMF-beta, human, recombinant Recombinant Human Glia Maturation Factor beta 10 µg C-60432 

GMF-gamma, human, recombinant Recombinant Human Glia Maturation Factor gamma 20 µg C-60433 

GRO-alpha (MGSA, CXCL1), human, 

recombinant 

GRO-beta (MIP-2, CXCL2), human, 

recombinant 

GRO-gamma (CXCL3), human, 

recombinant 

Growth Hormone (GH), human, 

recombinant 

HB-EGF, human, recombinant (Plant) 

HCC-1 (66 aa, CCL14), human, 

recombinant 

HCC-1 (72 aa, CCL14), human, 

recombinant 

Recombinant Human Growth Regulated Oncogene alpha 

(Melanoma Growth Stimulating Activity, CXCL1) 

Recombinant Human Growth Regulated Oncogene beta 

(CXCL2) 

Recombinant Human Growth Regulated Oncogene 

gamma (CXCL3) 

25 µg C-65430 

10 µg C-65440 

10 µg C-65450 

Recombinant Human Growth Hormone (GH) 100 µg C-67335 

Recombinant Human Heparin Binding EGF-like Growth 


Recombinant Human Hemofiltrate CC-Chemokine 1 

(66 amino acid variant, CCL14) 

Recombinant Human Hemofiltrate CC-Chemokine 1 

(72 amino acid variant, CCL14) 

50 µg C-60190A 

10 µg C-67712 

10 µg C-67710 

HDGF, human, recombinant Recombinant Human Hepatoma-Derived Growth Factor 20 µg C-67720 

Heregulin beta-1, human, recombinant 

HGF, human, recombinant (CHO) 

HGF, human, recombinant (Sf9) 

HGF-A, human, recombinant 

Recombinant Human Heregulin beta-1 

(HRG1-beta1, NRG1-beta1) 

Recombinant Human Hepatocyte Growth Factor 


Recombinant Human Hepatocyte Growth Factor 


Recombinant Human Hepatocyte Growth Factor, A 

Chain 

50 µg C-67511 

10 µg C-64531 

10 µg C-64530 

10 µg C-64533 





HGF-B, human, recombinant Recombinant Human Hepatocyte Growth Factor, B Chain 10 µg C-64534 

HVEM/Fc, human, recombinant 

Recombinant Human Herpes Virus Entry Mediator 

(Fc Chimera) 

100 µg C-68115 

I-309 (CCL1), human, recombinant Recombinant Human I-309 (CCL1) 10 µg C-67810 

IFN-alpha 1, human, recombinant 

(E. coli) 

IFN-alpha 1a, human, recombinant 

(E. coli) 

IFN-alpha 1b, human, recombinant 

(E. coli) 


(E. coli) 


(Plant) 


(E. coli) 


(Yeast) 

IFN-beta 1a, human, recombinant 

(CHO) 

IFN-beta 1b, human, recombinant 

(E. coli) 

IFN-gamma, human, recombinant 

(E. coli) 

Recombinant Human Interferon alpha 1 (E. coli-derived) 20 µg C-60577 

Recombinant Human Interferon alpha-1a 


Recombinant Human Interferon alpha-1b 


Recombinant Human Interferon alpha-2a 


100 µg C-60578 

100 µg C-60579 

100 µg C-60580 

Recombinant Human Interferon alpha-2a (plant-derived) 100 µg C-60580A 

Recombinant Human Interferon alpha-2b 


100 µg C-60581 

Recombinant Human Interferon alpha-2b (yeast-derived) 50 µg C-60582 

Recombinant Human Interferon beta-1a 


10 µg C-60591 

Recombinant Human Interferon beta-1b (E. coli-derived) 10 µg C-60590 

Recombinant Human Interferon gamma (E. coli-derived) 100 µg C-60724 

IFN-gamma, human, recombinant (Plant) Recombinant Human Interferon gamma (plant-derived) 100 µg C-60724A 

IFN-lambda-1 (IL-29), human, 

recombinant 

IFN-lambda-2 (IL-28A), human, 

recombinant 

Recombinant Human Interferon lambda-1 (IL-29) 20 µg C-60730 

Recombinant Human Interferon lambda-2 (IL-28A) 20 µg C-60732 

IFN-omega, human, recombinant Recombinant Human Interferon omega 100 µg C-60806 

IGF-I, human, recombinant (E. coli) 

IGF-I, human, recombinant (Plant) 

Recombinant Human Insulin Like Growth Factor I 




100 µg C-60840 

100 µg C-60840A 

IGF-II, human, recombinant Recombinant Human Insulin Like Growth Factor II 50 µg C-60960 

IGF-IR, human, recombinant 

IGF-BP1, human, recombinant 





Recombinant Human Insulin-Like Growth Factor I 

Receptor 

Recombinant Human Insulin Like Growth Factor 

Binding Protein 1 









5 µg C-60841 

20 µg C-60842 

20 µg C-60845 

25 µg C-60848 

20 µg C-60849 

25 µg C-60850 





116 



IGF-BP6, human, recombinant (E. coli) 

IGF-BP6, human, recombinant (InC) 



Binding Protein 6 (E. coli-derived) 


Binding Protein 6 (Hi-5 insect cell-derived) 



20 µg C-60853 

25 µg C-60854 

25 µg C-60855 

IL-1 alpha, human, recombinant (E. coli) Recombinant Human Interleukin 1-alpha (E. coli-derived) 10 µg C-61112 

IL-1 alpha, human, recombinant (Plant) Recombinant Human Interleukin 1-alpha (plant-derived) 10 µg C-61112A 

IL-1 beta, human, recombinant Recombinant Human Interleukin 1-beta 10 µg C-61120 

IL-1 RA, human, recombinant Recombinant Human Interleukin 1 Receptor Antagonist 100 µg C-61122 

IL-1RL1, human, recombinant Recombinant Human Interleukin-1 Receptor Like-1 10 µg C-61124 

IL-2, human, recombinant (E. coli) Recombinant Human Interleukin 2 (E. coli-derived) 50 µg C-61240 

IL-2, human, recombinant (Plant) Recombinant Human Interleukin 2 (plant-derived) 50 µg C-61240A 

IL-2, human, recombinant (Yeast) Recombinant Human Interleukin 2 (yeast-derived) 20 µg C-61242 

sIL-2R alpha (CD25), human, 

recombinant 

Recombinant Human soluble Interleukin 2 Receptor 

alpha (CD25) 

25 µg C-63333 

IL-3 CC, human, recombinant Recombinant Human Interleukin 3 (for Cell Culture) ~ 30 µg C-61301 



IL-3, human, recombinant (Sf9) Recombinant Human Interleukin 3 


10 µg C-61325 


IL-4 CC, human, recombinant Recombinant Human Interleukin 4 for (for Cell Culture) ~ 5 µg C-61401 

IL-4, human, recombinant (CHO) Recombinant Human Interleukin 4 (CHO cell-derived) 10 µg C-61450 






recombinant 

Recombinant Human Soluble Interleukin 4 Receptor 

alpha (CD124) 

15 µg C-61422 



IL-6 CC, human, recombinant Recombinant Human Interleukin 6 (for Cell Culture) ~ 10 µg C-61601 




sIL-6 Receptor (CD126), human, 

recombinant 

Recombinant Human soluble Interleukin 6 

Receptor (CD126) 

20 µg C-61631 




IL-8 (CXCL8), human, recombinant 

(Pichia) 

IL-8 (CXCL8, 72 aa), human, 

recombinant 

Recombinant Human Interleukin 8 

(CXCL8, Pichia-derived) 


(CXCL8, 72 amino acid variant) 

25 µg C-61829 

25 µg C-61831 





IL-8 (CXCL8, 77 aa), human, 

recombinant 


(CXCL8, 77 amino acid variant) 

25 µg C-61830 





IL-11, human, recombinant Recombinant Human Interleukin 11 10 µg C-62120 


IL-12-p40, human, recombinant (CHO) Recombinant Human Interleukin 12-p40 


IL-12-p80, human, recombinant (InC) Recombinant Human Interleukin 12-p80 

(insect cell-derived) 

10 µg C-62215 

10 µg C-62218 

IL-13 (variant), human, recombinant Recombinant Human Interleukin 13 (Variant) 10 µg C-62314 



IL-16 (121 aa), human, recombinant Recombinant Human Interleukin 16 (121 amino acid variant) 10 µg C-62611 

IL-16 (130 aa), human, recombinant Recombinant Human Interleukin 16 (130 amino acid variant) 10 µg C-62613 


IL-17, human, recombinant Recombinant Human Interleukin 17A 25 µg C-62710 

IL-17A/F, human, recombinant Recombinant Human Interleukin 17A/F Heterodimer 10 µg C-62719 

IL-17B, human, recombinant Recombinant Human Interleukin 17B 25 µg C-62712 

IL-17D, human, recombinant Recombinant Human Interleukin 17D 25 µg C-62714 

IL-17E (IL-25, SF20), human, 



recombinant (Plant) 

Recombinant Human Interleukin 17E 

(IL-25, SF20; E. coli-derived) 

Recombinant Human Interleukin 17E 

(IL-25, SF20; plant-derived) 

25 µg C-62715 

50 µg C-62715A 

IL-17F, human, recombinant Recombinant Human Interleukin 17F 25 µg C-62718 









IL-32 alpha, human, recombinant Recombinant Human Interleukin 32 alpha 10 µg C-62953 


Insulin, human, recombinant (E. coli) Recombinant Human Insulin (E. coli-derived) 25 mg C-60212 

Insulin, human, recombinant (Yeast) Recombinant Human Insulin (yeast-derived) 10 mg C-60214 

IP-10 (CXCL10), human, recombinant Recombinant Human IFN Inducible Protein 10 (CXCL10) 25 µg C-65520 

IRF-1, human, recombinant Recombinant Human Interferon Regulatory Factor 1 50 µg C-60600 






118 




IRF-4, human, recombinant Recombinant Human Interferon Regulatory Factor 4 

(MUM-1) 

2 µg C-60603 



I-TAC (CXCL11), human, recombinant 

KGF (FGF-7), human, recombinant 

(E. coli) 


(Plant) 

Recombinant Human Interferon-inducible T-cell alpha 

Chemoattractant (CXCL11) 

Recombinant Human Keratinocyte Growth Factor 

(FGF-7; E. coli-derived) 


(FGF-7; plant-derived) 

20 µg C-67820 

10 µg C-63820 

10 µg C-63820A 

KGF-2, human, recombinant Recombinant Human Keratinocyte Growth Factor 2 25 µg C-63823 

Klotho, human, recombinant Recombinant Human Klotho 20 µg C-67520 

LAG-1 (CCL4L1), human, recombinant Recombinant Human Lymphocyte Activation Gene 1 

(CCL4L1) 

LD78 beta (CCL3L1), human, 

recombinant 

LEC (CCL16, NCC-4), human, 

recombinant 

20 µg C-65842 

Recombinant Human LD78 beta (CCL3L1) 20 µg C-65840 

Recombinant Human Liver-Expressed Chemokine 

(CCL16, NCC-4) 

20 µg C-67830 

Leptin, human, recombinant Recombinant Human Leptin (Anti-Obesity Protein) 1 mg C-68232 

Leptin Receptor, human, recombinant Recombinant Human Leptin Binding Domain 50 µg C-68233 

LeukinFeron, human, native Human LeukinFeron 10,000 

IU 

LFA-3 (CD58), human, recombinant 

Recombinant Human Lymphocyte Function Associated 

Antigen 3 (CD58), Fusion Protein 

C-67515 

100 µg C-65400 

LIGHT, human, recombinant Recombinant Human LIGHT 15 µg C-66720 

LIF, human, recombinant (E. coli) 

LIF, human, recombinant (Plant) 

Recombinant Human Leukemia Inhibitory Factor 




10 µg C-60050 

10 µg C-60050A 

L-selectin (CD62L), human, recombinant Recombinant Human L-selectin (CD62L) 10 µg C-69220 

Lymphotactin, human, recombinant Recombinant Human Lymphotactin 20 µg C-65620 

sLYVE-1, human, recombinant (Sf9) 

Recombinant Human Lymphatic Vessel Endothelial 

Hyaluronan Receptor 1 (Sf9 insect cell-derived) 

25 µg C-67355 

Maspin, human, recombinant Recombinant Human Maspin 25 µg C-65630 

MCP-1 (MCAF, CCL2), human, 

recombinant 

Recombinant Human Monocyte Chemotactic Protein 1 

(MCAF, CCL2) 

MCP-2 (CCL8), human, recombinant Recombinant Human Monocyte Chemotactic Protein 2 

(CCL8) 


(CCL7) 


(CCL13) 

M-CSF, human, recombinant (E. coli) 

M-CSF, human, recombinant (Plant) 

MDC (CCL22, 67 aa), human, 

recombinant 

Recombinant Human Macrophage Colony Stimulating 

Factor (E. coli-derived) 



Recombinant Human Macrophage Derived Chemokine 


20 µg C-64030 

10 µg C-65722 

10 µg C-65723 

10 µg C-65724 

10 µg C-60442 

10 µg C-60442A 

20 µg C-67910 





MDC (CCL22, 69 aa), human, 

recombinant 

MEC (CCL28), human, recombinant 

MIA, human, recombinant 

MIA-2, human, recombinant 

b2-Microglobulin (b2-M), human, 

recombinant 

Recombinant Human Macrophage Derived Chemokine 


Recombinant Human Mucosae Associated Epithelial 


Recombinant Human Melanoma Inhibitory Activity 

Protein 

Recombinant Human Melanoma Inhibitory Activity 

Protein 2 

Recombinant Human beta2-Microglobulin 

(b2-M, BDGF-2, CRG-8; E. coli-derived) 

20 µg C-67920 

20 µg C-67940 

20 µg C-65815 

20 µg C-65818 

50 µg C-69302 

Midkine, human, recombinant Recombinant Human Midkine 20 µg C-67950 

MIF (active), human, recombinant Recombinant Human Mesoderm Inducing Factor (active) 10 µg C-65814 

MIF, human, recombinant Recombinant Human Mesoderm Inducing Factor 25 µg C-65813 

MIG (CXCL9), human, recombinant 

MIP-1 alpha (CCL3), human, 




MIP-1 beta (CCL4), human, 

recombinant 

MIP-3 (CCL23), human, 

recombinant 


recombinant 

MIP-3 beta (CCL19), human, 

recombinant 


recombinant 


recombinant 

Recombinant Human Monokine Induced by Interferon 

gamma (CXCL9) 

Recombinant Human Macrophage Inflammatory Protein 

1-alpha (CCL3; E. coli-derived) 


1-alpha (CCL3; plant-derived) 


1-beta (CCL4) 


3 (MPIF-1, CCL23) 


3-alpha (CCL20) 


3-beta (CCL19) 


4 (CCL18) 


5 (CCL15) 

20 µg C-65811 

20 µg C-65833 

10 µg C-65833A 

10 µg C-65822 

20 µg C-65860 

20 µg C-65866 

20 µg C-65877 

10 µg C-65888 

25 µg C-65890 

MMP-1, human, recombinant Recombinant Human Matrix Metalloproteinase 1 10 µg C-64102 




MMP-8, human, recombinant Recombinant Human Matrix Metalloproteinase 8 1 unit C-64112 


MMP-13, human, recombinant Recombinant Human Matrix Metalloproteinase 13 2.5 µg C-64118 

Myostatin, human, recombinant Recombinant Human Myostatin 10 µg C-65920 

Myostatin Propetide, human, 

recombinant 

Recombinant Human Myostatin Propeptide 25 µg C-65921 

Nanog, human, recombinant Recombinant Human Nanog 20 µg C-64325 

NAP-2 (CXCL7), human, recombinant Recombinant Human Neutrophil Activating Protein 2 

(CXCL7) 

Neuregulin-1 (NRG-1), human, 


Recombinant Human Neuregulin 1 

(NRG-1, Heregulin beta-2; E. coli-derived) 

10 µg C-64320 

50 µg C-67512 





120 








(NRG-1, Heregulin beta-2; plant-derived) 

50 µg C-67512A 

Neuritin, human, recombinant Recombinant Human Neuritin 20 µg C-67525 

Neuroglobin, human, recombinant Recombinant Human Neuroglobin 10 µg C-60210 

Neuroserpin, human, recombinant Recombinant Human Neuroserpin 25 µg C-65265 

Neurturin (NTN), human, recombinant Recombinant Human Neurturin (NTN) 20 µg C-68110 

beta-NGF, human, recombinant (CHO) 

beta-NGF, human, recombinant (E. coli) 

Recombinant Human Nerve Growth Factor beta 


Recombinant Human Nerve Growth Factor beta 


20 µg C-66130 

20 µg C-66129 

NNT-1 (BCSF-3), human, recombinant Recombinant Human Novel Neurotrophin-1 (BCSF-3) 10 µg C-66421 

NOGGIN, human, recombinant (E. coli) Recombinant Human NOGGIN (E. coli-derived) 20 µg C-67350 

NOGGIN, human, recombinant (HEK) Recombinant Human NOGGIN (HEK cell-derived) 20 µg C-67351 

NOV, human, recombinant 

Recombinant Human Nephroblastoma Overexpressed 

Gene 

NP-1, human, recombinant Recombinant Human Neutrophil Peptide 1 

(alpha-Defensin) 

20 µg C-64335 

20 µg C-64330 

NT-3, human, recombinant Recombinant Human Neurotrophin 3 10 µg C-66425 

NT-4, human, recombinant Recombinant Human Neurotrophin 4 10 µg C-66426 

Omentin, human, recombinant Recombinant Human Omentin 10 µg C-65025 

Oncostatin M (209 aa), human, 

recombinant 

Oncostatin M (227 aa), human, 

recombinant 

OPG (OCIF), human, recombinant 

Osteopontin (SPP-1), human, 

recombinant 

Recombinant Human Oncostatin M 

(OSM; 209 amino acid variant) 

Recombinant Human Oncostatin M 


Recombinant Human Osteoprotegerin 

(Osteoclastogenesis Inhibitory Factor) 

10 µg C-65019 

10 µg C-65020 

50 µg C-63750 

Recombinant Human Osteopontin (SPP-1) 50 µg C-67530 

OSTF-1, human, recombinant Recombinant Human Osteoclast Stimulating Factor 1 50 µg C-68050 

OTOR, human, recombinant Recombinant Human Otoraplin 20 µg C-64342 

OX40L, human, recombinant Recombinant Human OX40 Ligand 10 µg C-64350 

PAF-AH, human, recombinant 

Recombinant Human Platelet Activating Factor 

(Acetylhydrolase) 

20 µg C-65940 

PAI-2, human, recombinant Recombinant Human Plasminogen Activator Inhibitor 2 10 µg C-63792 

PDGF-AA, human, recombinant (E. coli) 

PDGF-AA, human, recombinant (Pichia) 

Recombinant Human Platelet Derived Growth Factor AA 


Recombinant Human Platelet Derived Growth Factor AA 

(Pichia-derived) 

10 µg C-63021 

10 µg C-63028 

PDGF-AB, human, recombinant Recombinant Human Platelet Derived Growth Factor AB 10 µg C-63020 

PDGF-BB, human, recombinant (E. coli) 

PDGF-BB, human, recombinant (Plant) 

PDGF-BB, human, recombinant (Yeast) 

Recombinant Human Platelet Derived Growth Factor BB 





(yeast-derived) 

10 µg C-63022 

10 µg C-63024A 

10 µg C-63024 





PDGF-CC, human, recombinant Recombinant Human Platelet Derived Growth Factor CC 20 µg C-63026 

PEDF, human, recombinant (E. coli) 

PEDF, human, recombinant (HEK) 

Recombinant Human Pigment Epithelium Derived Factor 


Recombinant Human Pigment Epithelium Derived Factor 


20 µg C-63030 

10 µg C-63031 

Periostin, human, recombinant Recombinant Human Periostin 10 µg C-60045 

Persephin, human, recombinant Recombinant Human Persephin 20 µg C-66320 

PF-4 (CXCL4), human, recombinant Recombinant Human Platelet Factor 4 (CXCL4) 20 µg C-65930 

PGRN, human, recombinant Recombinant Human Progranulin 10 µg C-60080 

Pleiotrophin, human, recombinant Recombinant Human Pleiotrophin (PTN) 20 µg C-66640 

PLGF-1, human, recombinant (E. coli) Recombinant Human Placenta Growth Factor 1 


PLGF-1, human, recombinant (Sf9) Recombinant Human Placenta Growth Factor 1 


PLGF-2, human, recombinant (E. coli) Recombinant Human Placenta Growth Factor 2 


PLGF-2, human, recombinant (Sf9) Recombinant Human Placenta Growth Factor 2 


25 µg C-66022 

10 µg C-66021 

10 µg C-66023 

10 µg C-66020 

PLGF-3, human, recombinant Recombinant Human Placenta Growth Factor 3 25 µg C-66024 

ProBMP-2, human, recombinant Recombinant Human Pro Bone Morphogenetic Protein 2 10 µg C-67308 

Prokinectin-2, human, recombinant Recombinant Human Prokinectin 2 20 µg C-63035 

Prolactin, human, recombinant (E. coli) Recombinant Human Prolactin (E. coli-derived) 50 µg C-60035 

Prolactin, human, recombinant (Plant) Recombinant Human Prolactin (plant-derived) 50 µg C-60035A 

Prolactin Receptor (Prlr), human, 

recombinant 

Prolactin Receptor (Prlr), human, 

recombinant 

Recombinant Human Prolactin Receptor 

(Prlr, GST-tagged) 

5 µg C-60038 

Recombinant Human Prolactin Receptor (Prlr) 50 µg C-60039 

Pro-NGF, human, recombinant Recombinant Human Pro-Nerve Growth Factor 10 µg C-66133 

Prox-1 (fragment), human, recombinant Recombinant Human Prox-1 (fragment) 5 µg C-67400 

P-selectin (CD62P), human, recombinant Recombinant Human P-selectin (CD62P) 10 µg C-69222 

PTHrP, human, recombinant 

sRANK Receptor, human, recombinant 

sRANKL, human, recombinant (E. coli) 

sRANKL, human, recombinant (Plant) 

Recombinant Human Parathyroid Hormone-like 

Related Protein 

Recombinant Human soluble Receptor Activator of 

NF-Kappa-B Receptor (TNFRSF11A) 


NF-Kappa-B Ligand (E. coli-derived) 


NF-Kappa-B Ligand (plant-derived) 

50 µg C-66650 

100 µg C-63201 

10 µg C-63200 

10 µg C-63200A 

RANTES (CCL5), human, recombinant Recombinant Human RANTES (CCL5) 20 µg C-64130 

RBP-4, human, recombinant Recombinant Human Retinol Binding Protein 4 20 µg C-66685 

Relaxin-2, human, recombinant Recombinant Human Relaxin 2 25 µg C-66674 

Relaxin-3, human, recombinant Recombinant Human Relaxin 3 25 µg C-66676 

RELM beta, human, recombinant Recombinant Human Resistin-like Molecule beta 25 µg C-66665 

Resistin, human, recombinant Recombinant Human Resistin 25 µg C-66670 





122 



SCF, human, recombinant (E. coli) Recombinant Human Stem Cell Factor (E. coli-derived) 10 µg C-63120 



SCF, human, recombinant (Sf9) 

Recombinant Human Stem Cell Factor (Sf9 insect 

cell-derived) 

10 µg C-63125 

SCGF-alpha, human, recombinant Recombinant Human Stem Cell Growth Factor alpha 10 µg C-63130 

SCGF-beta, human, recombinant Recombinant Human Stem Cell Growth Factor beta 10 µg C-63140 

SDF-1 alpha (CXCL12), human, 

recombinant 

SDF-1 beta (CXCL12), human, 

recombinant 

Recombinant Human Stromal Cell Derived Factor 1-alpha 

(CXCL12) 

Recombinant Human Stromal Cell Derived Factor 1-beta 

(CXCL12) 

10 µg C-67220 

10 µg C-67320 

Shh, human, recombinant (E. coli) Recombinant Human Sonic Hedgehog (E. coli-derived) 25 µg C-66679 

Slit2-N, human, recombinant Recombinant Human Slit2-N 10 µg C-68322 

TACI, human, recombinant 

Recombinant Human Transmembrane Activator and 

CAML Interactor 

20 µg C-66690 

TAFA-2, human, recombinant Recombinant Human TAFA 2 20 µg C-63620 

TARC (CCL17), human, recombinant 

TECK (CCL25), human, recombinant 

Recombinant Human Thymus and Activation Regulated 


Recombinant Human Thymus Expressed Chemokine 

(CCL25) 

20 µg C-68010 

20 µg C-68020 

TFF-1, human, recombinant Recombinant Human Trefoil Factor 1 20 µg C-65050 



TGF-alpha, human, recombinant Recombinant Human Transforming Growth Factor alpha 100 µg C-63535 

TGF-beta 1, human, recombinant (HEK) 

TGF-beta 1, human, recombinant (CHO) 

TGF-beta 2, human, recombinant (HEK) 

TGF-beta 2, human, recombinant (Sf9) 

TGF-beta 3, human, recombinant (E. 

coli) 

TGF-beta 3, human, recombinant (CHO) 

TGF-betaR2, human, recombinant 

Recombinant Human Transforming Growth Factor beta-1 












Recombinant Human Transforming Growth Factor Beta 

Receptor 2 

5 µg C-63501 

5 µg C-63504 

5 µg C-63502 

5 µg C-63506 

10 µg C-63507 

10 µg C-63508 

10 µg C-63540 

Thrombomodulin (CD141) Recombinant Human Thrombomodulin (CD141) 10 µg C-65062 

sTIE-1/Fc Chimera, human, 


sTIE-2/Fc Chimera, human, 


TIMP-1, human, recombinant 

TIMP-2, human, recombinant 

Recombinant Human soluble TIE-1/Fc Chimera (Sf9 

insect cell-derived) 

Recombinant Human soluble TIE-2/Fc Chimera (Sf9 

insect cell-derived) 

Recombinant Human Tissue Inhibitor of 

Metalloproteinases 1 

Recombinant Human Tissue Inhibitor of 

Metalloproteinases 2 

20 µg C-68025 

20 µg C-68028 

10 µg C-68560 

10 µg C-68562 

TL-1A, human, recombinant Recombinant Human TNF-like 1A (TNFSF15) 20 µg C-65065 





TLR-3 (CD283), human, recombinant Recombinant Human Toll-like Receptor 3 (CD283) 25 µg C-68035 

TNF-alpha M, human, recombinant 

TNF-alpha, human, recombinant (E. coli) 

TNF-alpha, human, recombinant (E. coli) 

TNF-alpha, human, recombinant (Plant) 

TNF-alpha, human, recombinant (Yeast) 

TNF-alpha, human, recombinant (Yeast) 

TNF-beta, human, recombinant (E. coli) 

TNF-beta, human, recombinant (InC) 

TNF-beta, human, recombinant (Plant) 

sTNF-R1 (CD120a), human, 

recombinant 

sTNF-R2 (CD120b), human, 

recombinant 

TNF-Receptor Fusion Protein, human, 

recombinant 

TNFRSF17 (CD269), human, 

recombinant 

TPO, human, recombinant (CHO) 

TPO, human, recombinant (E. coli) 

TRAIL (114-281 aa), human, 

recombinant 

TRAIL (Apo2L), human, recombinant 

sTRAIL Receptor-1 (DR4), human, 

recombinant 

sTRAIL Receptor-2 (DR5), human, 

recombinant 

Recombinant Human Tumor Necrosis Factor alpha, 

Mutant/Variant (151 amino acid variant) 

Recombinant Human Tumor Necrosis Factor alpha 










Recombinant Human Tumor Necrosis Factor beta 



(insect cell-derived) 



Recombinant Human soluble Tumor Necrosis Factor 

Receptor Type I (CD120a) 


Receptor Type II (CD120b) 

Recombinant Human Tumor Necrosis Factor Receptor 

Fusion Protein 

Recombinant Human TNFRSF17 (Tumor Necrosis Factor 

Receptor Superfamily 17, CD269) 

Recombinant Human Thrombopoietin 

(Mp1-ligand, MGDF, CHO cell-derived) 

Recombinant Human Thrombopoietin 

(Mp1-ligand, MGDF; E. coli-derived) 

Recombinant Human TNF-Related Apoptosis 

Inducing Ligand (Apo2L, 114-281 amino acid variant) 

Recombinant Human TNF Related Apoptosis 

Inducing Ligand (Apo2L) 

Recombinant Human soluble TNF Related Apoptosis 

Inducing Ligand Receptor 1 (DR4) 

Recombinant Human soluble TNF Related Apoptosis 

Inducing Ligand Receptor 2 (DR5) 

50 µg C-63723 

10 µg C-63722 

50 µg C-63719 

50 µg C-63720A 

10 µg C-63720 

50 µg C-63721 

20 µg C-63731 

20 µg C-63733 

10 µg C-63731A 

20 µg C-63725 

20 µg C-63760 

50 µg C-63726 

20 µg C-63738 

10 µg C-65114 

10 µg C-65112 

10 µg C-62599 

50 µg C-63600 

50 µg C-63601 

50 µg C-63602 

TSG , human, recombinant Recombinant Human Twisted Gastrulation Protein 50 µg C-63766 

TSLP, human, recombinant Recombinant Human Thymic Stromal Lymphopoietin 10 µg C-63770 

TWEAK, human, recombinant 

TWEAK Receptor, human, recombinant 

Recombinant Human TNF-Related Weak Inducer of 

Apoptosis 

Recombinant Human TNF-Related Weak Inducer of 

Apoptosis Receptor 

25 µg C-63610 

25 µg C-63612 

Vaspin, human, recombinant Recombinant Human Vaspin 25 µg C-68325 

VCAM-1 (CD106), human, recombinant Recombinant Human Vascular Cell Adhesion Molecule 1 

(CD106) 

10 µg C-60063 





124 



VEGF-121, human, recombinant (E. coli) 

Recombinant Human Vascular Endothelial Cell 

Growth Factor 121 (E. coli-derived) 

10 µg C-64410 

VEGF-121, human, recombinant (Plant) 


Growth Factor 121 (plant-derived) 

10 µg C-64410A 

VEGF-121, human, recombinant (Sf9) 


Growth Factor 121 (Sf9-insect cell-derived) 

10 µg C-64409 

VEGF-145, human, recombinant (Sf9) 


Growth Factor 145 (Sf9-insect cell-derived) 

5 µg C-64418 

VEGF-165, human, recombinant (CHO) 


Growth Factor 165 (CHO cell-derived) 

10 µg C-64424 

VEGF-165, human, recombinant (E. coli) 



10 µg C-64420 

VEGF-165, human, recombinant (HEK) 


Growth Factor 165 (HEK cell-derived) 

10 µg C-64423 




10 µg C-64420A 

VEGF-165, human, recombinant (Yeast) 


Growth Factor 165 (yeast-derived) 

10 µg C-64421 

VEGF-189, human, recombinant 


Growth Factor 189 

20 µg C-64415 

VEGF-B, human, recombinant 


Growth Factor B 

20 µg C-64411 

VEGF-C, human, recombinant 


Growth Factor C 

20 µg C-64412 

VEGF-D, human, recombinant 


Growth Factor D 

10 µg C-64414 

VEGF-E, Orf virus, recombinant 

Recombinant Orf Virus Vascular Endothelial Cell 

Growth Factor E 

10 µg C-64416 

VEGF-I (TNFSF15), human, recombinant 


Growth Factor Inhibitor (TNFSF15) 

10 µg C-64480 

sVEGFR-1 (flt-1, D1-3) human, 


Recombinant Human soluble Vascular Endothelial 

Growth Factor Receptor 1, Domain 1-3 

(flt-1; Sf9 insect cell-derived) 

5 µg C-64430 






5 µg C-64440 






5 µg C-64450 



sVEGFR-1 (flt-1, D7-Fc) human, 


sVEGFR-1 (flt-1, native), human, 






(flt-1, Fc chimera; Sf9 insect cell-derived) 


Growth Factor Receptor 1 

(flt-1, native, Sf9 insect cell-derived) 


Growth Factor Receptor 1 


10 µg C-64465 

20 µg C-64425 

5 µg C-64460 





sVEGFR-2 (sKDR, D1-D7) human, 


sVEGFR-2 (sKDR-Fc, D7) human, 






(sKDR; Sf9 insect cell-derived) 



(sKDR, Fc chimera; Sf9 insect cell-derived) 


Growth Factor Receptor-3 


5 µg C-64470 

10 µg C-64472 

10 µg C-64428 

Visfatin, human, recombinant Recombinant Human Visfatin 25 µg C-68330 

Vitronectin, human, recombinant Recombinant Human Vitronectin 250 µg C-69200 

WIF-1, human, recombinant Recombinant Human WNT Inhibitory Factor 1 5 µg C-68340 

WISP-1, human, recombinant 

WISP-3, human, recombinant 

Recombinant Human WNT-1-Inducible Signaling 

Pathway Protein 1 

Recombinant Human WNT-1-Inducible Signaling 

Pathway Protein 3 

20 µg C-68310 

20 µg C-68312 

WNT-1, human, recombinant Recombinant Human WNT-1 10 µg C-68316 

n Mouse 


Acrp30, mouse, recombinant (E. coli) 

Acrp30, mouse, recombinant (HEK) 

Recombinant Mouse Adiponectin (Adipocyte 

Complement Related Protein of 30 kDa; E. coli-derived) 

Recombinant Mouse Adiponectin (Adipocyte 

Complement Related Protein of 30 kDa, glycosylated; 

HEK cell-derived) 

25 µg D-66629 

10 µg D-66628 

gAcrp30, mouse, recombinant Recombinant Mouse globular Acrp30 (Adipolean) 25 µg D-66630 

Apolipoprotein-J, mouse, recombinant Recombinant Mouse Apolipoprotein J (ApoJ, Clusterin) 10 µg D-68250 

APRIL, mouse, recombinant 

BCA-1 (BLC, CXCL13), mouse, 

recombinant 

Recombinant Mouse APRIL (A Proliferation Inducing 

Ligand; TALL-2, TRDL-1a, TNFSF13) 

Recombinant Mouse Human B-cell Attracting 

Chemokine 1 (CXCL13) 

20 µg D-63745 

20 µg D-65240 

Betacellulin (BTC), mouse, recombinant Recombinant Mouse Betacellulin (BTC) 20 µg D-60510 

C-10 (CCL6), mouse, recombinant Recombinant Mouse C-10 (CCL6) 10 µg D-65330 

Cardiotrophin-1 (CT-1), mouse, 

recombinant 

Recombinant Mouse Cardiotrophin-1 (CT-1) 10 µg D-67410 

CD14, mouse, recombinant (CHO) Recombinant Mouse CD14 (CHO cell-derived) 2 µg D-63324 

sCD40 Ligand, mouse, recombinant 

sCD105 (Endoglin), mouse, recombinant 

(Sf9) 

CTACK (CCL27), mouse, recombinant 

Recombinant Mouse soluble CD40 Ligand 

(TNFSF5, TRAP, CD154, gp39) 

Recombinant Mouse soluble CD105 

(Endoglin, Sf9-insect cell-derived) 

Recombinant Mouse Cuteaneous T-cell Attracting 


25 µg D-63100 

25 µg D-60058 

20 µg D-65350 

CXCL16, mouse, recombinant Recombinant Mouse CXC Ligand 16 25 µg D-66610 





126 



EBI-3, mouse, recombinant Recombinant Mouse Epstein-Barr Virus Induced Gene 3 10 µg D-68112 

EGF, mouse, recombinant Recombinant Mouse Epidermal Growth Factor 500 µg D-60170 

Eotaxin (CCL11), mouse, recombinant Recombinant Mouse Eotaxin (CCL11) 20 µg D-65320 

Eotaxin-2 (CCL24), mouse, recombinant Recombinant Mouse Eotaxin-2 (CCL24) 20 µg D-65332 

Exodus-2 (CCL21), mouse, recombinant Recombinant Mouse Exodus-2 (CCL21) 20 µg D-66512 

FGF-1 (FGF-a), mouse, recombinant Recombinant Mouse acidic Fibroblast Growth Factor 1 

(FGF-a) 

FGF-2 (FGF-b), mouse, recombinant Recombinant Mouse basic Fibroblast Growth Factor 2 

(FGF-b) 

50 µg D-60340 

50 µg D-60240 

FGF-9, mouse, recombinant Recombinant Mouse Fibroblast Growth Factor 9 10 µg D-60220 

FGF-21, mouse, recombinant Recombinant Mouse Fibroblast Growth Factor 21 10 µg D-60494 

flt-3 Ligand, mouse, recombinant Recombinant Mouse flt-3 Ligand 10 µg D-67110 



G-CSF, mouse, recombinant 

Recombinant Mouse Granulocyte Colony Stimulating 

Factor 

10 µg D-60431 

GDNF, mouse, recombinant Recombinant Mouse Glial Derived Neurotropic Factor 10 µg D-66312 

GM-CSF, mouse, recombinant (E. coli) 

GM-CSF, mouse, recombinant (Plant) 

Recombinant Mouse Granulocyte/Macrophage Colony 

Stimulating Factor (E. coli-derived) 



10 µg D-60420 

10 µg D-60420A 

Granzyme B, mouse, recombinant Recombinant Mouse Granzyme B 10 µg D-69156 

Growth Hormone (GH), mouse, 

recombinant 

Recombinant Mouse Growth Hormone (GH) 100 µg D-67335 

IFN-gamma, mouse, recombinant Recombinant Mouse Interferon gamma 100 µg D-60724 

IFN-lambda-2 (IL28A), mouse, 

recombinant 

Recombinant Mouse Interferon lambda-2 (IL-28A) 20 µg D-60732 

IGF-I, mouse, recombinant Recombinant Mouse Insulin Like Growth Factor I 50 µg D-60840 

IL-1 alpha, mouse, recombinant Recombinant Mouse Interleukin 1-alpha 10 µg D-61112 

IL-1 beta, mouse, recombinant Recombinant Mouse Interleukin 1-beta 10 µg D-61122 

IL-2, mouse, recombinant Recombinant Mouse Interleukin 2 20 µg D-61220 









IL-12, mouse, recombinant (CHO) Recombinant Mouse Interleukin 12 (CHO cell-derived) 10 µg D-62210 




IL-17, mouse, recombinant Recombinant Mouse Interleukin 17A 25 µg D-62710 





IL-17A/F, mouse, recombinant Recombinant Mouse Interleukin 17A/F Heterodimer 10 µg D-62719 

IL-17E (IL-25, SF20), mouse, 

recombinant 

Recombinant Mouse Interleukin 17E (IL-25, SF20) 25 µg D-62715 

IL-17F, mouse, recombinant Recombinant Mouse Interleukin-17F 25 µg D-62718 





IL-25 (SF20), mouse, recombinant Recombinant Mouse Interleukin 25 (SF20) 10 µg D-63010 

IL-27 (p28), mouse, recombinant Recombinant Mouse Interleukin 27 (p28) 10 µg D-62930 



IP-10 (CXCL10), mouse, recombinant Recombinant Mouse IFN Inducible Protein 10 (CXCL10) 25 µg D-65340 

I-TAC (CXCL11), mouse, recombinant 

KC (GRO-alpha, CXCL1), mouse, 

recombinant 

Recombinant Mouse Interferon-inducible T-cell alpha 

Chemoattractant (CXCL11) 

Recombinant Mouse Keratinocyte Chemoattractant 

(GRO-alpha, CXCL1) 

20 µg D-66710 

20 µg D-65420 

Leptin, mouse, recombinant Recombinant Mouse Leptin 1 mg D-68230 

LIF, mouse, recombinant (E. coli) 

LIF, mouse, recombinant (Plant) 

Recombinant Mouse Leukemia Inhibitory Factor 




10 µg D-60050 

10 µg D-60050A 

LIGHT, mouse, recombinant Recombinant Mouse LIGHT 20 µg D-66720 

LIX (CXCL5, 70 aa), mouse, recombinant 

LIX (CXCL5, 93 aa), mouse, recombinant 

Lungkine (CXCL15), mouse, 

recombinant 

sLYVE-1, mouse, recombinant (Sf9) 

MCP-1 (JE, CCL2), mouse, recombinant 

Recombinant Mouse Lipopolysaccharide Induced CXC 

Chemokine (CXCL5, 70 amino acid variant) 

Recombinant Mouse Lipopolysaccharide Induced CXC 

Chemokine (CXCL5, 93 amino acid variant) 

20 µg D-66519 

20 µg D-66520 

Recombinant Mouse Lungkine (CXCL15) 20 µg D-66525 

Recombinant Mouse soluble Lymphatic Vessel Endothelial 

Hyaluronan Receptor 1 (Sf9 insect cell-derived) 

Recombinant Mouse Monocyte Chemotactic Protein 

(JE, CCL2) 

MCP-2 (CCL8), mouse, recombinant Recombinant Mouse Monocyte Chemotactic Protein 2 

(CCL8) 


(CCL7) 


(CCL12) 

M-CSF, mouse, recombinant 

MDC (CCL22), mouse, recombinant 

MEC (CCL28), mouse, recombinant 

Recombinant Mouse Macrophage Colony Stimulating 

Factor 

Recombinant Mouse Macrophage Derived Chemokine 

(CCL22) 

Recombinant Mouse Mucosae-Associated Epithelial 


25 µg D-67355 

10 µg D-64030 

20 µg D-65722 

10 µg D-65723 

20 µg D-65725 

10 µg D-60442 

20 µg D-67910 

20 µg D-67940 





128 





MIG (CXCL9), mouse, recombinant 

MIP-1 alpha (CCL3), mouse, 

recombinant 

MIP-1 beta (CCL4), mouse, recombinant 

MIP-1 gamma (CCL9/10), mouse, 

recombinant 

MIP-2 (GRO-beta, CXCL2), mouse, 

recombinant 

MIP-3 alpha (CCL20), mouse, 

recombinant 

MIP-3 beta (CCL19), mouse, 

recombinant 

Recombinant Mouse Monokine Induced by 

Interferon-gamma (CXCL9) 

Recombinant Mouse Macrophage Inflammatory 

Protein 1-alpha (CCL3) 


Protein 1-beta (CCL4) 


Protein 1-gamma 


Protein 2 (CXCL2) 


Protein 3-alpha (CCL20) 


Protein 3-beta (CCL19) 

20 µg D-65811 

10 µg D-65833 

10 µg D-65822 

20 µg D-65844 

20 µg D-65430 

20 µg D-65866 

20 µg D-65877 

Neuropoietin (NP), mouse, recombinant Recombinant Mouse Neuropoietin (NP, NPO) 25 µg D-65890 

beta-NGF, mouse, recombinant Recombinant Mouse Nerve Growth Factor beta 20 µg D-66130 

NOGGIN, mouse, recombinant Recombinant Mouse NOGGIN 20 µg D-67350 

NT-3, mouse, recombinant Recombinant Mouse Neurotrophin 3 10 µg D-66425 

PDGF-AA, mouse, recombinant Recombinant Mouse Platelet Derived Growth Factor AA 10 µg D-63021 

PDGF-BB, mouse, recombinant Recombinant Mouse Platelet Derived Growth Factor BB 10 µg D-63022 

Persephin, mouse, recombinant Recombinant Mouse Persephin 20 µg D-66320 

PF-4 (CXCL4), mouse, recombinant Recombinant Mouse Platelet Factor 4 (CXCL4) 20 µg D-65930 

PLGF-2, mouse, recombinant (E. coli) Recombinant Mouse Placenta Growth Factor 2 


10 µg D-66022 

Prolactin, mouse, recombinant Recombinant Mouse Prolactin 50 µg D-60035 

sRANKL, mouse, recombinant 

Recombinant Mouse soluble Receptor Activator of 

NF-Kappa B Ligand 

10 µg D-63200 

RANTES (CCL5), mouse, recombinant Recombinant Mouse RANTES (CCL5) 20 µg D-64130 

RELM-alpha, mouse, recombinant Recombinant Mouse Resistin-like Molecule alpha 25 µg D-66660 

RELM-beta, mouse, recombinant Recombinant Mouse Resistin-like Molecule beta 25 µg D-66665 

RELM-gamma, mouse, recombinant Recombinant Mouse Resistin-like Molecule gamma 25 µg D-66668 

Resistin, mouse, recombinant Recombinant Mouse Resistin 25 µg D-66670 

SCF, mouse, recombinant (E. coli) Recombinant Mouse Stem Cell Factor (E. coli-derived) 10 µg D-63120 

SCF, mouse, recombinant (Plant) Recombinant Mouse Stem Cell Factor (plant-derived) 10 µg D-63120A 

SDF-1 alpha (CXCL12), mouse, 

recombinant 

SDF-1 beta (CXCL12), mouse, 

recombinant 

Recombinant Mouse Stromal Cell-Derived Factor 1-alpha 

(CXCL12) 

Recombinant Mouse Stromal Cell-Derived Factor 1-beta 

(CXCL12) 

10 µg D-67220 

10 µg D-67222 

Shh, mouse, recombinant Recombinant Mouse Sonic Hedgehog 25 µg D-66680 

sTIE-1 / Fc Chimera, mouse, 


sTIE-2 / Fc Chimera, mouse, 


TNF-alpha, mouse, recombinant (E. coli) 

Recombinant Mouse soluble TIE-1/Fc Chimera 

(Sf9-insect cell-derived) 

Recombinant Mouse soluble TIE-2/Fc Chimera 

(Sf9-insect cell-derived) 

Recombinant Mouse Tumor Necrosis Factor alpha 


20 µg D-68025 

20 µg D-68028 

20 µg D-63720 





TNF-alpha, mouse, recombinant 

(S. lividans) 

Recombinant Mouse Tumor Necrosis Factor alpha 

(S. lividans-derived) 

20 µg D-63722 

TPO, mouse, recombinant Recombinant Mouse Thrombopoietin 10 µg D-65112 

TRAIL (Apo2L), mouse, recombinant 

VEGF-121, mouse, recombinant 

VEGF-164, mouse, recombinant (InC) 

VEGF-164, mouse, recombinant (Plant) 

VEGF-165, mouse, recombinant (E. coli) 

VEGF-165, mouse, recombinant (Sf9) 

Recombinant Mouse TNF-Related Apoptosis 

Inducing Ligand (Apo2L) 

Recombinant Murine Vascular Endothelial Cell 

Growth Factor 121 

Recombinant Mouse Vascular Endothelial 

Growth Factor 164 (insect cell-derived) 





Recombinant Mouse Vascular Endothelial Growth Factor 

165 (Sf9-insect cell-derived) 

50 µg D-63600 

10 µg D-64409 

20 µg D-64408 

10 µg D-64408A 

10 µg D-64410 

10 µg D-64423 

Visfatin, mouse, recombinant Recombinant Mouse Visfatin 25 µg D-68330 

WNT-3a, mouse, recombinant Recombinant Mouse WNT-3a 10 µg D-68318 

n Rat 


Apolipoprotein-J, rat, recombinant Recombinant Rat Apolipoprotein J (ApoJ, Clusterin) 10 µg E-68250 

CNTF, rat, recombinant Recombinant Rat Ciliary Neurotrophic Factor 25 µg E-66210 

EGF, rat, recombinant Recombinant Rat Epidermal Growth Factor 100 µg E-60170 

FGF-2 (FGF-b), rat, recombinant Recombinant Rat basic Fibroblast Growth Factor 2 (FGF-b) 50 µg E-60240 

FGF-9, rat, recombinant Recombinant Rat Fibroblast Growth Factor 9 10 µg E-60390 

Fractalkine (CX3CL1), rat, recombinant Recombinant Rat Fractalkine (CX3CL1) 20 µg E-67510 

GDNF, rat, recombinant Recombinant Rat Glial Derived Neurotropic Factor 10 µg E-66312 

GM-CSF, rat, recombinant 

GRO-alpha (KC, CXCL1), rat, 

recombinant 

GRO-beta (MIP-2, CXCL2), rat, 

recombinant 

Recombinant Rat Granulocyte/Macrophage Colony 

Stimulating Factor 

Recombinant Rat Growth Regulated Oncogene alpha 

(KC, CXCL1) 

Recombinant Rat Growth Regulated Oncogene beta 

(MIP-2, CXCL2) 

10 µg E-60420 

25 µg E-65420 

25 µg E-65430 

Growth Hormone (GH), rat, recombinant Recombinant Rat Growth Hormone (GH) 100 µg E-67335 

IFN-gamma, rat, recombinant Recombinant Rat Interferon gamma 100 µg E-60730 

IGF-I, rat, recombinant Recombinant Rat Insulin Like Growth Factor I 50 µg E-60840 

IL-1 alpha, rat, recombinant Recombinant Rat Interleukin 1-alpha 10 µg E-61112 

IL-1 beta, rat, recombinant Recombinant Rat Interleukin 1-beta 10 µg E-61120 

IL-2, rat, recombinant Recombinant Rat Interleukin 2 20 µg E-61220 


IL-3 beta, rat, recombinant Recombinant Rat Interleukin 3-beta 20 µg E-61342 





130 










IL-13 (109 aa), rat, recombinant Recombinant Rat Interleukin 13 (109 amino acid variant) 10 µg E-62314 

IL-13 (113 aa), rat, recombinant Recombinant Rat Interleukin 13 (113 amino acid variant) 10 µg E-62312 


IL-17, rat, recombinant Recombinant Rat Interleukin 17A 25 µg E-62710 

IL-17E (IL-25), rat, recombinant Recombinant Rat Interleukin 17E (IL-25, SF20) 25 µg E-62715 

IL-17F, rat, recombinant Recombinant Rat Interleukin 17F 25 µg E-62718 


IP-10 (CXCL10), rat, recombinant Recombinant Rat IFN Inducible Protein 10 (CXCL10) 25 µg E-65340 

Leptin, rat, recombinant Recombinant Rat Leptin 1 mg E-68230 



MCP-1 (CCL2), rat, recombinant 

Recombinant Rat Monocyte Chemoattractant 

Protein-1 (CCL2) 

10 µg E-64030 

M-CSF, rat, recombinant Recombinant Rat Macrophage Colony Stimulating Factor 10 µg E-60442 

MIP-1 alpha (CCL3), rat, recombinant 

MIP-1 beta (CCL4), rat, recombinant 

Recombinant Rat Macrophage Inflammatory Protein 

1-alpha (CCL3) 

Recombinant Rat Macrophage Inflammatory Protein 

1-beta (CCL4) 

20 µg E-65833 

20 µg E-65822 

Oncostatin M, rat, recombinant Recombinant Rat Oncostatin M (OSM) 10 µg E-65020 

Prolactin, rat, recombinant Recombinant Rat Prolactin 50 µg E-60035 

Prolactin Receptor (Prlr), rat, 

recombinant 

sRANKL, rat, recombinant 

Recombinant Rat Prolactin Receptor (Prlr) 50 µg E-60039 

Recombinant Rat soluble Receptor Activator of 

NF-Kappa-B Ligand 

10 µg E-63200 

RANTES (CCL5), rat, recombinant Recombinant Rat RANTES (CCL5) 20 µg E-64130 

Resistin, rat, recombinant Recombinant Rat Resistin 25 µg E-66670 

SCF, rat, recombinant Recombinant Rat Stem Cell Factor 10 µg E-63120 

SDF-1 alpha (CXCL12), rat, recombinant 

SDF-1 beta (CXCL12), rat, recombinant 

Recombinant Rat Stromal Cell Derived Factor 1-alpha 

(CXCL12) 

Recombinant Rat Stromal Cell Derived Factor 1-beta 

(CXCL12) 

10 µg E-67220 

10 µg E-67320 

TNF-alpha, rat, recombinant Recombinant Rat Tumor Necrosis Factor alpha 20 µg E-63720 

TPO, rat, recombinant Recombinant Rat Thrombopoietin (Mp1-ligand, MGDF) 10 µg E-65112 

VEGF-165, rat, recombinant Recombinant Rat Vascular Endothelial Growth Factor A 10 µg E-64411 

VEGF-C, rat, recombinant Recombinant Rat Vascular Endothelial Growth Factor C 10 µg E-64412 

VEGF-C152S, rat, recombinant 

Recombinant Rat Vascular Endothelial Growth Factor C 


10 µg E-64415 




7.2 Animal-free Cytokines and Growth Factors 

PromoKine’s animal-free cytokines and 

growth factors have been produced by 

molecular farming using barley grain 

as the production host - which has the 

G.R.A.S. (Generally Recognized As Safe) 

status from the FDA. These cytokines 

are free of animal components, serum, 

endotoxins, and antibiotics, as well as 

human or animal infectious agents or 

other endogenous mammalian contaminants 

(e.g. traces of unwanted growth 

factors and cytokines). As these proteins 

have been expressed in barley seed endosperm, 

they show very low proteolytic, 

pyrogenic, and inflammatory activity 

compared to other commercially available 

proteins such as those produced in bacteria, 

yeast, insect, or mammalian cells. 

In addition, they retain proper eukaryotic 

folding and glycosylation resulting in an 

increased stability and biological activity. 

Degradation due to protease carryover 

as well as unwanted cell responses (e.g. 

proliferation, chemokine release and 

inflammatory cascades) or cell growth 

inhibition caused by contaminating serum 

components (serum carryover), 

endotoxins or bacterial, viral, and prion 

infectious agents are also eliminated or 

at least extremely reduced. 

OD (490 nm) 

1.6 

1.5 

Control fraction 

PromoKine´s animal-free VEGF-121 

Fig. 1: Cell proliferation 

assay using PromoKine´s 

animal-free VEGF-121 and 

HUVEC cells (see PromoCell 

catalog, Cat.No. C-12200). 

1.4 

1.3 

1.2 

0.1 

1.0 

0.9 

0.8 

0.7 

0.0 10-³ 10-² 10-¹ 1.0 10¹ 10² 

ng/ml VEGF-121 

10³ 

n Human 


Angiogenin-1, human, recombinant 

(Plant) 

Recombinant Human Angiogenin-1 (plant-derived) 50 µg C-60110A 

EMAP-2, human, recombinant 

(Plant) 


Polypeptide II (plant-derived) 

10 µg C-65390A 


(Plant) 


(Plant) 





50 µg C-60340A 

50 µg C-60240A 

flt-3 Ligand, human, recombinant (Plant) Recombinant Human flt-3 Ligand (plant-derived) 10 µg C-67110A 



G-CSF, human, recombinant (Plant) 



10 µg C-60434A 

For prices please see www.promokine.info/pricelist 


132 





GDNF, human, recombinant (Plant) 

GM-CSF, human, recombinant (Plant) 

HB-EGF, human, recombinant (Plant) 


(Plant) 

IFN-gamma, human, recombinant 

(Plant) 

IGF-I, human, recombinant (Plant) 





Recombinant Human Heparin Binding EGF-like 

Growth Factor (plant-derived) 

10 µg C-66312A 

10 µg C-60420A 

50 µg C-60190A 

Recombinant Human Interferon alpha-2a (plant-derived) 100 µg C-60580A 

Recombinant Human Interferon gamma (plant-derived) 100 µg C-60724A 



100 µg C-60840A 

IL-1 alpha, human, recombinant (Plant) Recombinant Human Interleukin 1-alpha (plant-derived) 10 µg C-61112A 











Recombinant Human Interleukin 17E (IL-25, SF20; 

plant-derived) 

50 µg C-62715A 




(Plant) 

LIF, human, recombinant (Plant) 

M-CSF, human, recombinant (Plant) 





PDGF-BB, human, recombinant (Plant) 








1-alpha (CCL3; plant-derived) 


(NRG-1, Heregulin beta-2; plant-derived) 



10 µg C-63820A 

10 µg C-60050A 

10 µg C-60442A 

10 µg C-65833A 

50 µg C-67512A 

10 µg C-63024A 

Prolactin, human, recombinant (Plant) Recombinant Human Prolactin (plant-derived) 50 µg C-60035A 

sRANKL, human, recombinant (Plant) 

TNF-alpha, human, recombinant (Plant) 

TNF-beta, human, recombinant (Plant) 


NF-Kappa-B Ligand (plant-derived) 





10 µg C-63200A 

50 µg C-63720A 

10 µg C-63731A 








Factor 121 (plant-derived) 


Factor 165 (plant-derived) 

10 µg C-64410A 

10 µg C-64420A 

n Mouse 


GM-CSF, mouse, recombinant (Plant) 

LIF, mouse, recombinant (Plant) 





10 µg D-60420A 

10 µg D-60050A 

SCF, mouse, recombinant (Plant) Recombinant Mouse Stem Cell Factor (plant-derived) 10 µg D-63120A 

VEGF-164, mouse, recombinant (Plant) 

Recombinant Mouse Vascular Endothelial Growth Factor 

164 (plant-derived) 

10 µg D-64408A 





134 


7.3 CD Antigens 

n Human 


CD1A, human, recombinant Recombinant Human CD1A 2 µg C-63300 


sCD4, human, recombinant Recombinant Human soluble CD4 10 µg C-60056 




CD11b, human, recombinant Recombinant Human CD11b 2 µg C-63318 







recombinant 


alpha (CD25) 

25 µg C-63333 


CD31 (PECAM-1), human, recombinant Recombinant Human CD31 (PECAM-1) 2 µg C-63339 




CD54 (ICAM-1), human, recombinant 

Recombinant Human Intercellular Adhesion Molecule-1 

(CD54) 

50 µg C-63356 


LFA-3 (CD58), human, recombinant 

Recombinant Human Lymphocyte Function Associated 

Antigen 3 (CD58), Fusion Protein 

100 µg C-65400 

E-selectin (CD62E), human, recombinant Recombinant Human E-selectin (CD62E) 10 µg C-69217 

L-selectin (CD62L), human, recombinant Recombinant Human L-selectin (CD62L) 10 µg C-69220 

P-selectin (CD62P), human, recombinant Recombinant Human P-selectin (CD62P) 10 µg C-69222 



sFas Receptor (CD95), human, 

recombinant 

Recombinant Human soluble Fas Receptor 

(CD95, TNFRSF6) 

20 µg C-66623 







VCAM-1 (CD106), human, 

recombinant 

sTNF-R1 (CD120a), human, 

recombinant 

sTNF-R2 (CD120b), human, 

recombinant 


recombinant 


(Endoglin, E. coli-derived) 


(Endoglin; Sf9 insect cell-derived) 

Recombinant Human Vascular Cell Adhesion Molecule 1 

(CD106) 


Receptor Type I (CD120a) 


Receptor Type II (CD120b) 

Recombinant Human Soluble Interleukin 4 Receptor 

alpha (CD124) 

5 µg C-60059 

25 µg C-60058 

10 µg C-60063 

20 µg C-63725 

20 µg C-63760 

15 µg C-61422 





sIL-6 Receptor (CD126), human, 

recombinant 


(CD126) 

20 µg C-61631 

4-1BB sReceptor (CD137), human, 

recombinant 

Recombinant Human 4-1BB soluble Receptor (CD137) 20 µg C-66551 

4-1BBL (CD137L), human, recombinant Recombinant Human 4-1BB Ligand (CD137L) 20 µg C-66550 

Thrombomodulin (CD141) Recombinant Human Thrombomodulin (CD141) 10 µg C-65062 



CD152 (CTLA-4), human, recombinant 

sCD40 Ligand (CD154), human, 

recombinant 

TNFRSF17 (CD269), human, 

recombinant 

Recombinant Human CD152 

(Cytotoxic T-lymphocyte-Associated Antigen 4) 

Recombinant Human soluble CD40 Ligand 

(TRAP, CD154) 

Recombinant Human TNFRSF17 

(Tumor Necrosis Factor Receptor Superfamily 17, CD269) 

50 µg C-63396 

50 µg C-63100 

20 µg C-63738 

TLR-3 (CD283), human, recombinant Recombinant Human Toll-like Receptor 3 (CD283) 25 µg C-68035 

n Mouse 


CD14, mouse, recombinant (CHO) Recombinant Mouse CD14 (CHO cell-derived) 2 µg D-63324 

sCD40 Ligand, mouse, recombinant 

sCD105 (Endoglin), mouse, 


Recombinant Mouse soluble CD40 Ligand (TNFSF5, 

TRAP, CD154, gp39) 

Recombinant Mouse soluble CD105 

(Endoglin, Sf9-insect cell-derived) 

25 µg D-63100 

25 µg D-60058 


7.4 Cytokines and Growth Factors for Stem Cell Research 

Stem cells play a central role in the de- 

Mammalian stem cells are generally 

generative organs. Most adult stem cells 

velopment of multicellular organisms 

comprised of two types: Embryonic stem 

are lineage-restricted (multipotent) and 

and diverse regeneration processes 

cells (ESCs) are derived from the epiblast 

characterized by a limited self-renewal 

throughout their lifes, e.g. replacing 

tissue of the inner cell mass of an early 

and differentiation capacity usually 

diseased and damaged tissues. They are 

stage embryo (blastocyst) and are con- 

restricted to cell types of the tissue in 

unspecialized precursor cells characterized 

by their abilities of self-renewal 

and pluripotency, that means they can 

undergo numerous rounds of cell division 

maintaining the undifferentiated 

sidered pluripotent and capable to differentiate 

into any cell type of the adult 

body. Adult stem cells (also known as 

somatic stem cells) are undifferentiated 

cells found in the tissues of children and 

which they reside. In contrast, induced 

pluripotent stem cells (iPSCs) are not 

adult stem cells but specialised, somatic 

adult cells (e.g. epithelial cells) that have 

been genetically reprogrammed to pluri- 



state and are able to develop into differ- 

adults. Their main function is to replace 

potent cells which are in many respects 

ent types of cells such as skin, muscle or 

dead or damaged specialiced cells and 

similar to natural pluripotent stem cells 

blood cells. 

to maintain the normal turnover of re- 

such as embryonic stem cells. 

PromoKine provides a wide range of cytokines and growth factors which are important in stem cell 

research. For a complete list, please visit www.cytokines.info/stemcells

Microbial Detection 

8 

and Elimination 

Cell culture contamination is a commonly occurring problem 

that affects many of the cell’s biochemical and immunological 

properties, and it leads to unsatisfactory and unreliable results. 

Therefore, regularly testing cell cultures for contamination with 

mycoplasmas/bacteria is essential. PromoKine provides highly 

sensitive and user-friendly test kits for this purpose as well as 

products for the effective elimination and prevention of mycoplasma/eubacteria 

contamination.

138 


8 

Microbial Detection 

and Elimination 

8.1 Mycoplasma Test Kits ............... 139 

8.2 Mycoplasma Elimination Solutions. ..... 140 

8.3 Bacteria Test Kit. ................... 142 

8.4 Disinfectants ...................... 143

8 Microbial Detection and Elimination 139 

8.1 Mycoplasma Test Kits 

Applications 

Mycoplasma detection in cell culture, 

cell/virus stocks, and medium 

Conventional and real-time PCR 

Features 

Highly sensitive (detects 10 - 15 

mycoplasmas per sample volume) 

Fast, reproducible, and reliable results 

Applicable for all cell/tissue culturerelevant 

mycoplasma species 

Positive and internal controls included 

Convenient protocol and easy sample 

preparation 

Mycoplasmas are parasitic bacteria that 

frequently contaminate tissue cultures 

and consequently also virus stocks, 

vaccines, and other biological materials 

produced in cells. The contamination can 

originate from serum, laboratory staff, 

other contaminated cultures, or the donors 

from which the cells have been harvested, 

and it often remains unnoticed. 

Although mycoplasmas do not cause 

visible cell damage, they seriously affect 

cell growth and metabolism, transfection 

efficiency, protein and monoclonal 

antibody synthesis, cytokine secretion, 

immunological properties, signal transduction, 

virus proliferation and can even 

cause DNA and RNA damage. 

Since mycoplasmas are very small and lack 

cell walls, detection using a conventional 

light microscope is nearly impossible. In 

addition, cloudiness in the medium, typical 

for bacterial or yeast contaminations, 

does not occur even at high mycoplasma 

concentrations. PCR on the other hand 

is a highly sensitive, specific, and rapid 

method for the detection of mycoplasma 

contamination in culture supernatant, 

cell/virus stocks, and medium. All cell/ 

tissue culture relevant mycoplasma species 

can be detected in one assay including 

M. fermentans, M. hyorhinis, M. 

arginini, M. orale, M. salivarium, M. 

hominis, M. pulmonis, and M. pirum. In 

addition, M. pneumoniae, Acholeplasma 

laidlawii, M. synoviae, and Ureaplasma 

species can be detected. 

PCR Mycoplasma Test Kit I/C 

The PCR Mycoplasma Test Kit I/C utilizes 

conventional PCR technology for convenient 

and specific detection of more than 

25 contaminating mycoplasma species. 

The primer set is specific for the highly 

conserved 16S-rRNA coding region in 

the mycoplasma genome. The test can 

be performed in 3 hours and includes 

internal control DNA (for verifying a successful 

PCR run) as well as positive control 

DNA. All components required for 

the PCR (primers, nucleotides, internal 

control DNA) come optimally premixed 

and lyophilized in PCR tubes (except 

the Taq polymerase). For setting up 

the PCR, simply cut off the number of 

tubes required, rehydrate with rehydration 

buffer (containing the Hot-Start Taq 

polymerase), and add the sample to be 

analyzed. Samples can be easily prepared 

by boiling the cell culture supernatant or 

other sample material. 

PCR Mycoplasma Test Kit I/RT 

The PCR Mycoplasma Test Kit I/RT utilizes 

real-time PCR technology for convenient 

and reproducible detection of 

contaminating mycoplasmas. It shows 

excellent sensitivity and specificity and 

enables reliable, quantitative PCR results 

extremely fast, particularly for routine 

testing for mycoplasma contamination. 

The primer and probe set is specific for 

the highly conserved 16S-rRNA coding 

region in the mycoplasma genome. It 

contains FRET probes emitting fluorescent 

light at 520 nm and is compatible 

with most common Real-Time PCR cyclers 

(e.g. Roche Light Cycler ® 1 and 2, 

ABI Prism ® , Cepheid Smart Cycler ® , and 

Eppendorf Mastercycler ® ep realplex). 

The test includes internal control DNA 

(for verifying a successful PCR run) as 

well as positive control DNA. All components 

required for real time PCR 

(primers, probes, nucleotides, and internal 

control DNA) come lyophilized 

in PCR tubes, except the Hot-Start Taq 

polymerase which is dissolved in reaction 

buffer. Two kit variants (kit A for 

e.g. Light Cycler, kit B for e.g. ABI Prism) 

are provided which have been optimized 

for different Real Time PCR instruments. 

For recommended kit variant, please see 

www.promokine.info where you can 

download the manual. 

PCR 

100 µl cell culture 

supernatant 

Boil for 10 minutes 

and centrifuge briefly 

2 µl supernatant 

Analyze 5 µl of 

the PCR sample 

Fig. 1: Mycoplasma detection with the PromoKine PCR Mycoplasma Test Kit I/C is easy and convenient: Boil 100 µl cell culture 

supernatant or other sample material for 10 minutes; centrifuge briefly and add 2 µl supernatant to the reaction cup with the rehydrated 

PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis. When using 

the PCR Mycoplasma Test Kit II, the sample is directly added to the ready-to-use, complete reaction mix to set up the PCR. 

8 Microbial 

Detection and 

Elimination

140 

8 Microbial Detection and Elimination 

PCR Mycoplasma Test Kit III 

The PCR Mycoplasma Test Kit III utilizes 

conventional PCR technology for convenient 

and highly sensitive detection of 

mycoplasma. The primer set is specific for 

the highly conserved 16S-rRNA coding 

region in the mycoplasma genome. Thus, 

the kit reliably detects all mycoplasma 

commonly found in cell culture, including 

the Acholeplasma and Spiroplasma 

genera. The kit has been positively tested 

against all mycoplasma strains listed in the 

European Pharmacopoeia, but may detect 

even more mycoplasma species (~70-120 

species). It does not detect microorganisms 

such as Streptococcus sp., Clostridium 

sp. and Lactobacillus sp. which are 

phylogenetically closely related. 

The kit includes all components required 

for PCR (nucleotides, primers, a highperformance 

Taq polymerase, and an 

agarose gel loading dye), all premixed at 

optimal concentrations and in a ready-touse 

and ready-to-load 1x master mix. The 

test takes approximately 3 hours to run 

and obtain clear results. The kit also contains 

positive control DNA for easy result 

verification as well as an internal control 

DNA to verify a successful PCR run. 

M 1 2 3 4 5 6 

1 2 3 4 5 6 7 M 

479 bp 

270 bp 

260 bp 

160 bp 

Fig. 2 Top: Detection of mycoplasmas 

with PCR Mycoplasma Test Kit I/C. 

M: 100 bp DNA ladder 

1: Positive control 

2-6: 10; 100; 1,000; 10,000; 100,000 

mycoplasmas/sample volume 

Internal control DNA: 479 bp band 

Positive sample/control: 270 bp band 

Bottom: Detection of mycoplasmas 

with PCR Mycoplasma Test Kit III. 

1-4: A. laidlawii, S. citri, M. synoviae, 

and M. orale (50 cfu/ml each) 

5: Cell culture supernatant, not infected 

6: Negative control 

7: Positive control 

M: DNA size standard 

Internal control DNA: 160 bp band 

Positive sample/control: 260-270 bp band 


PCR Mycoplasma Test Kit I/C 

24 tests 

48 tests 

96 tests 

PK-CA-91-1024 

PK-CA-91-1048 

PK-CA-91-1096 

PCR Mycoplasma Test Kit III 100 assays PK-CA92-100 

PCR Mycoplasma Test Kit I/RT; Variant A 25 assays PK-CA91-3025A 

PCR Mycoplasma Test Kit I/RT; Variant B 25 assays PK-CA91-3025B 

RTU DNA Ladder I (100 bp) 0.5 ml PK-MB713-001N 

RTU DNA Ladder II (1000 bp) 0.5 ml PK-MB713-002N 


Please see Chapter 1.11 for our ready-to-use DNA Ladders. PromoKine also provides fluorescent stains for mycoplasma detection, please see Chapter 1. 

8.2 Mycoplasma Elimination Solutions 

Mycoplasma contamination seriously af- 

are disinfected (see chapter 8.4 for dis- 

not affect mycoplasmas. Therefore, 

fects cell growth and cell physiology lead- 

infectant sprays). However, if the af- 

PromoKine offers two highly effective 

ing to biased and unreliable experimental 

fected cells are unique or the result of 

methods to get rid of mycoplasma con- 

8 Microbial 

Detection and 

Elimination 

results. When mycoplasma contamination 

is detected, the contaminated cells 

are ideally discarded and all equipment, 

e.g. work benches, CO 2 

incubators, etc., 

extensive work, thorough elimination of 

the contaminating mycoplasmas is necessary. 

Standard antibiotics commonly 

used in cell culture (e.g. Pen/Strep) do 

tamination: Mycoplasma-EX, a combination 

of non-antibiotic and antibiotic 

reagents, and BIOMYC 1-3, three highly 

effective antibiotics.


Mycoplasma-EX 

Applications 

Mycoplasma elimination in cell/tissue 

culture 

Mycoplasma elimination in virus 

stocks 

Features 

Highly effective against mycoplasma 

species commonly found in cell 

cultures 

Very short treatment time 

Very low cytotoxicity 

Ready to use 

Easy handling 

Mycoplasma-EX combines a non-antibiotic 

and a thoroughly adjusted antibiotic 

treatment for the effective elimination of 

mycoplasmas and other microorganisms 

in cell/tissue culture and virus stocks. It 

eliminates mycoplasmas by directly killing 

them and not just by inhibiting their 

growth, while showing extremely low 

toxicity for the cells in culture. 

Mycoplasma-EX is effective with only 

one treatment, destroying mycoplasmas 

rapidly and permanently. It is applicable 

for most cell lines (e.g. Vero, BHK21, 

GBK, ML, Hep2, 293, CRFK, H9, Molt4, 

MT-4, Jurkat) as well as virus stocks 

(e.g. SHV-1, BHV-1, HSV-1, VSV, SFV, 

FCV, MEV) and highly effective against 

M. orale, M. hyorhinis, M. hominis, 

Acholeplasma laidlawii, M. arginini, 

M. fermentans, and other mycoplasma 

species usually encountered as contaminants 

in cell cultures. 

BIOMYC Antibiotic 

Solutions 

Applications 

Mycoplasma elimination in cell/tissue 

culture 

Features 

Highly effective against mycoplasma 

species commonly found in cell 

cultures 

Low cytotoxicity 

Ready to use 

Easy handling 

Antibiotics that affect bacterial cell walls 

(e.g. penicillin) are ineffective against 

mycoplasmas, which lack a cell wall. 

Other antibiotics such as tylosin, neomycin, 

tetracycline, or gentamicin are 

only effective against a few mycoplasma 

species commonly found in cell cultures. 

In addition, they frequently just reduce 

the concentration of mycoplasmas rather 

than completely eliminating them. As 

soon as treatment is discontinued, contamination 

will recur. 

BIOMYC-1 and 2 

BIOMYC-1 and 2 are two antibiotics, 

which eliminate mycoplasmas very efficiently 

when used in combination. After 

a four-day treatment with BIOMYC-1, 

a three-day treatment with BIOMYC-2 

follows. This is repeated 2 to 3 times. 

Following this protocol, the mycoplasmas 

do not develop resistance to these 

antibiotics - a common occurrence with 

other antibiotic treatment methods. 

BIOMYC-1 is based on the antibiotic tiamutin 

which is produced by the fungus 

Pleurotus mutilus. BIOMYC-2 is based 

on the antibiotic minocycline, an improved 

derivative of tetracycline. 

BIOMYC-3 

BIOMYC-3 is based on the antibiotic 

ciprofloxacin, which is a member of 

the fluoroquinolone group and inhibits 

synthesis of DNA gyrase, an enzyme 

necessary for bacterial DNA supercoiling. 

Many mycoplasma species are 

sensitive to BIOMYC-3, including M. 

orale, M. hyorhinis, M. fermentans, and 

M. arginini, but also Acheloplasma laidlawii. 

These species are responsible for 

most mycoplasma contaminations in cell 

cultures. At the concentrations recommended 

for use, no cytotoxic effects on 

the cells in culture have been found and 

the treatment can be performed within 

12 days. 

Fig. 3: Electron microscope image of 

cells contaminated with mycoplasmas. 

Picture kindly provided by M. Ebert, 

Forschungszentrum für Medizintechnik 

und Biotechnologie, Erfurt, Germany. 


Mycoplasma-EX 3 treatments PK-CC91-4003 

BIOMYC-1 

10 ml 

20 ml 

100 ml 

PK-CC03-036-1D 

PK-CC03-036-1C 

PK-CC03-036-1B 

BIOMYC-2 

10 ml 

20 ml 

100 ml 

PK-CC03-037-1D 

PK-CC03-037-1C 

PK-CC03-037-1B 

BIOMYC-3 


10 ml 

20 ml 

100 ml 

PK-CC03-038-1D 

PK-CC03-038-1C 

PK-CC03-038-1B 

8 Microbial 

Detection and 

Elimination

142 

8 Microbial Detection and Elimination 

8.3 Bacteria Test Kit 

Applications 

Eubacteria detection in cell culture 

Eubacteria detection in cell/virus 

stocks 

Eubacteria detection in medium 

Features 

Highly sensitive (detects 12 eubacteria 

genomes per sample volume) 

Reproducible and reliable results 

Applicable for 45 eubacteria species 

Positive and internal controls included 

Convenient protocol and easy sample 

preparation 

Bacteria are frequently found as contaminants 

in cell cultures. However, cell 

cultures sometimes lack visual signs of 

bacterial contamination, e.g. fast decoloration 

of the medium. Moreover, it has 

been demonstrated that standard antibiotics 

are mostly ineffective against resistant 

bacterial infection but have a strong 

impact on the metabolism, growth, and 

differentiation of the cells in culture. 

Frequent screening of cell cultures for 

eubacteria contamination is indispensable 

to obtain reliable results. Therefore, 

PromoKine offers a PCR-based Bacteria 

Test Kit that has been developed for 

accurate and reliable in vitro diagnosis 

of 45 eubacteria species, many of 

them found as usual contaminants in 

cell cultures (e.g. Actinomyces, Bacillus, 

Enterococcus, Escherichia coli, Fusobacterium, 

Klebsiella, Lactobacillus, 

Micrococcus, Mycobacterium, Peptostreptococcus, 

Pseudomonas, Porphyromonas, 

Prevotella, Salmonella, Serratia, 

Staphylococcus, Streptococcus, 

etc.). The kit provides a highly sensitive, 

easy, and rapid method to detect eubacteria 

infection in various in situ biological 

materials, including cell cultures. Detection 

requires as little as 12 eubacteria 

genomes (~52 fg) per sample volume. 

The protocol can be completed within 

3.5 hours with a hands-on time of less 

than 40 minutes. The kit contains all necessary 

components for setting up a PCR 

including a positive control DNA as well 

as an internal control DNA to confirm a 

successfully performed PCR run. The kit 

does not amplify eukaryotic DNA. 

PCR 

200 - 500 µl cell 

culture supernatant 

Extract Bacterial 

DNA 

5 µl DNA sample 

Analyze 5 µl of 

the PCR sample 

Fig. 4: Eubacteria detection with the PromoKine PCR Bacteria Test Kit is easy and convenient: Purify bacterial DNA from 200 – 

500 µl cell culture supernatant by using an appropriate, commercial DNA extraction kit; add 5 µl sample DNA to the reaction cup 

with the premixed PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis. 


PCR Bacteria Test Kit 48 tests PK-CA-91-2048 


PromoKine also offers a Bacteria Live/Dead Staining Kit as well as fluorescent dyes for staining of bacteria. Please see Chapter 1. 

8 Microbial 

Detection and 

Elimination


8.4 Disinfectants 

Disinfection of all tissue culture equipment 

and work surfaces in the lab has to 

be performed regularly to prevent contamination. 

PromoKine offers different 

disinfectants ideal for laboratory surfaces 

and apparatuses, CO 2 

incubators, and 

water baths. 

Mycoplasma-EX Spray 

Applications 

Disinfection of all laboratory surfaces 

Disinfection of laboratory apparatuses 

Features 

Kills mycoplasmas within seconds 

Effective against most microorganisms 

Non-corrosive and non-cancerogenic 

Does not leave any residue 

Supplied in a refillable spray bottle 

Mycoplasma-EX Spray allows safe disinfection 

and cleaning of all laboratory surfaces 

and apparatuses, including clean 

and work benches, incubators, cell storage 

boxes, and liquid nitrogen containers. 

It utilizes the powerful Mycoplasma-EX 

reagent in combination with an 

alcohol-based solution. Besides its strong 

mycoplasmacidal activity, it is very effective 

against Staphylococcus aureus, 

Streptococcus faecalis, Escherichia coli, 

Proteus mirabilis, Pseudomonas aeruginosa, 

Candida albicans, Mycobacteria, 

HBV/HIV, Papova, Adeno, Polio and 

Vaccinia viruses. 

The effectiveness of Mycoplasma-EX 

Spray is further enhanced by its excellent 

detergent properties, so that clean 

and contamination-free surfaces can be 

achieved in one step. 

Pharmacidal Spray 

Applications 

Disinfection of incubators 

Features 

Free of mercury, formadeldehyde, 

phenol and alcohol 

Very effective against bacteria, fungi, 

spores, and viruses 

Non-corrosive and non-cancerogenic 

Does not leave any residue 

Supplied in a refillable spray bottle 

Pharmacidal Spray is a mercury-, formaldehyde-, 

phenol-, and alcohol-free 

disinfectant particularly intended for the 

disinfection of incubators. It is recommended 

to spray the incubators thoroughly 

every two weeks. 

Aquaguard 

Applications 

Disinfection of water trays in CO 2 

incubators 

Disinfection of water baths 

Features 

Very effective against bacteria, fungi, 

spores, and viruses 

Non-corrosive 

Non-toxic and non-volatile 

The water in an incubator or in a water 

bath is a major source of cell culture 

contamination, particularly with respect 

to mycoplasma contaminations, since 

mycoplasmas can survive in pure water. 

Aquaguard, a non-volatile disinfectant, is 

extremely effective in eliminating microorganisms 

in water. In addition, it does 

not cause damage to the stainless steel 

of the incubator or any plastic interior, 

and it does not cause any skin irritation 

when used as recommended. 

Aquaguard-1 is intended for disinfecting 

water in incubators, whereas Aquaguard-2 

is intended for water bath disinfection. 


Mycoplasma-EX Spray 

Pharmacidal Spray 

Aquaguard-1 (incubator) 

Aquaguard-2 (water bath) 

Mycoplasma Disinfection Kit I 

(Consists of Mycoplasma-Ex Spray, 

Aquaguard-1 and Aquaguard-2) 

Mycoplasma Disinfection Kit II 

(Consists of Pharmacidal Spray, 

Aquaguard-1 and Aquaguard-2) 


500 ml 

5 l 

1000 ml 

5 l 

100 ml 

10 x 100 ml 

50 ml 

10 x 50 ml 

PK-CC-91-5050 

PK-CC91-5050-5L 

PK-CC-110100 

PK-CC-110100-5L 

PK-CC-01-867-1B 

PK-CC01-867-10B 

PK-CC-01-916-1E 

PK-CC01-916-10E 

1 Kit PK-CC91-5060 

1 Kit PK-CC91-5070 

8 Microbial 

Detection and 

Elimination

144 

Alphabetical Index 


Description Page Description Page 

1 - 9 

1,25-Dihydroxy Vitamin D ELISA Kit, human 102 

4-1BB / CD137 ELISA Kit, human 93, 100 

4-1BB Receptor (CD137) EDK, human 103 

4-1BB sReceptor (CD137), human, recombinant 109, 135 

4-1BBL (CD137L), human, recombinant 109, 135 

4-Bromo A-23187 free acid 22 

5(6)-CFDA 21 

5(6)-CFDA, SE (CFSE) 21 

5-(and-6)-Carboxy-2',7'-dichlorofluorescein 21 

5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate 21 

5-(and-6)-Carboxyfluorescein 21 

5,5'-dibromo-Bapta (tetrapotassium salt) 20 

5,5'-difluoro-Bapta (tetrapotassium salt) 20 

5,5'-difluoro-Bapta AM 20 

5,5'-dimethyl BAPTA, AM ester 20 

5,5'-dimethyl BAPTA, tetrapotassium salt 20 

5-Carboxy-2',7'-dichlorofluorescein 21 

5-Carboxy-2',7'-dichlorosulfonefluorescein 21 

5-CFDA 21 

5-FAM 21 

6-CFDA 21 

6-Ckine ELISA Kit, mouse 97 

6-FAM 22 

7-AAD (7-aminoactinomycin D) 13 

90K (Mac-2BP) ELISA Kit, human 93 

α1-Antitrypsin (α1-Proteinase Inhibitor) ELISA Kit, human 102 

α1-Microglobulin (α1-M) ELISA Kit, human 102 

α2-Macroglobulin ELISA Kit, human 102 

A-23187 free acid 22 

Acceptor Vector 69 

Acridine Orange (AO) Solution 13 

Acrp30 (His), human, recombinant 109 

Acrp30, human, recombinant (E. coli) 109 

Acrp30, human, recombinant (HEK) 109 

Acrp30, human, recombinant (Sf9) 109 

Acrp30, mouse, recombinant (E. coli) 125 

Acrp30, mouse, recombinant (HEK) 125 

Actinomycin D 45 

Actinomycin D (10 mM) 45 

Activin A, human, recombinant 109 

Activin B, human, recombinant 109 

Adiponectin ELISA Kit, human 93 

ADMA direct ELISA Kit, mouse/rat 103 

ADMA ELISA Kit, human 102 

ADP Fluorometric & Colorimetric Quantitation Kit 25 

ADVASEP-7 (sulfobutyl b-cyclodextrin) 16 

AITRL, human, recombinant 109 

AK Cytotoxicity Kit 10 

Akt Activated Cell Lysate 43 

A 

Akt Activity Detection Kit 43 

Akt Inhibitor 43 

Akt Negative Control Cell Lysate 43 

Allophycocyanin (cross-linked), lyophilized 58 

Allophycocyanin, lyophilized 58 

Alpha-Bungarotoxin (from Bungarus multicinctus) 16 

AM1-43 16 

AM1-44 16 

AM2-10 16 

AM3-25 15, 16 

AM4-64 16 

AM4-66 16 

ANG-1, human, recombinant (HeLa) 109 

Ang-1, human, recombinant (Sf9) 109 

Ang-2, human, recombinant (Sf9) 109 

Angiogenin ELISA Kit, human 93 

Angiogenin-1, human, recombinant (Plant) 109, 131 

Angiopoietin-1 ELISA Kit, human 93 

Angiostatin ELISA Kit, human 93 

Angiostatin K1-3, human, recombinant 109 

Angiostatin K1-4, human 109 

ANGPTL-3, human, recombinant 109 

ANGPTL-4, human, recombinant 109 

Anisomycin 45 

Annexin V Binding Buffer 37 

Annexin V ELISA Kit, human 101 

Annexin V Unlabeled Reagent 37 

Annexin V-Biotin Detection Kit 37 

Annexin V-Biotin Reagent 37 

Annexin V-Cy3 Detection Kit 36 

Annexin V-Cy3 Detection Kit Plus 36 

Annexin V-Cy3 Reagent 37 

Annexin V-Cy5 Detection Kit 36 

Annexin V-Cy5 Reagent 37 

Annexin V-EGFP Detection Kit 36 

Annexin V-EGFP Reagent 37 

Annexin V-FITC Detection Kit 36 

Annexin V-FITC Detection Kit Plus 36 

Annexin V-FITC Reagent 37 

Annexin V-PE Detection Kit 37 

Annexin V-PE Detection Kit Plus 37 

Annexin V-PE Reagent 37 

Annexin V-PE-Cy5 Detection Kit 37 

Annexin V-PE-Cy5 Reagent 37 

anti-Annexin V ELISA Kit, human 101 

anti-IFN-alpha ELISA Kit, human 94 

APO-1 (Fas) ELISA Kit, human 101 

APO-A1, human, recombinant 109 

APO-E2, human, recombinant 109 



Apolipoprotein-D, human, recombinant 109 

Apolipoprotein-J, human, recombinant 109 

Apolipoprotein-J, mouse, recombinant 125

Alphabetical Index 145 


Apolipoprotein-J, rat, recombinant 129 

Apoptosis Inducer Set 45 

Apoptotic Cell Isolation Kit 45 

Apoptotic DNA Ladder Detection Kit I 33 

Apoptotic DNA Ladder Detection Kit I plus 33 

Apoptotic DNA Ladder Detection Kit II 33 

Apoptotic/Necrotic Cells Detection Kit 44 

Apoptotic/Necrotic/Healthy Cells Detection Kit 44 

Apo-SAA, human, recombinant 109 

Apo-SAA1, human, recombinant 109 

APRIL ELISA Kit, human 101 

APRIL, human, recombinant 110 

APRIL, mouse, recombinant 125 

Aquaguard-1 (incubator) 143 

Aquaguard-2 (water bath) 143 

Artemin, human, recombinant 110 

ATP Fluorometric & Colorimetric Quantitation Kit 25 

AXL ELISA Kit, human 93 

AXL ELISA Kit, mouse 97 

β2-Microglobulin (β2-M) ELISA Kit, human 102 

b2-Microglobulin (b2-M), human, recombinant 119 

Bacteria Live/Dead Staining Kit 9 

BAFF ELISA Kit, human 93, 101 

BAFF, human, recombinant 110 

BAFF-R, human, recombinant 110 

Bapta (tetracesium salt) 20 

Bapta (tetrapotassium salt) 20 

Bapta (tetrasodium salt) 20 

Bapta AM 20 

Bapta-FF (free acid) 20 

Bapta-FF AM 20 

BCA-1 (BLC, CXCL13), human, recombinant 110 

BCA-1 (BLC, CXCL13), mouse, recombinant 125 

BCECF 14, 22 

BCECF AM 14, 22 

BCECF AM Solution 14, 22 

Bcl-2 ELISA Kit, human 101 

BCMA, human, recombinant 110 

BD-1 (36 aa), human, recombinant 110 

BD-1 (47 aa), human, recombinant 110 

BD-1 EDK, human 103 


BD-2, human, recombinant 110 




BDNF ELISA Kit, human 93 

BDNF, human, recombinant 110 

Betacellulin (BTC), human, recombinant 110 

Betacellulin (BTC), mouse, recombinant 125 

Betacellulin EDK, human 103 

beta-Galactosidase Reporter Assay Kit 12 

beta-Galactosidase Staining Kit 12 

B 

beta-NGF EDK, human 104 

beta-NGF, human, recombinant (CHO) 120 

beta-NGF, human, recombinant (E. coli) 120 

beta-NGF, mouse, recombinant 128 

Beta-Secretase Fluorometric Assay Kit 23 

Beta-Secretase Inhibitor I 23 

Beta-Secretase Inhibitor II 23 

Betulinic acid 45 

Big Endothelin ELISA Kit, human 102 

Biocytin (e-biotinoyl –L-lysine) 17 

Biocytin-Hydrazide 17 

Bioluminescent Cell Viability Kit I (ATP) 8 

Bioluminescent Cell Viability Kit II (ADP/ATP) 8 

BIOMYC-1 141 

BIOMYC-2 141 

BIOMYC-3 141 

Biotin PCR Labeling Kit 53 

Biotin-4-Fluorescein 17 

Biotin-Allophycocyanin 59 

Biotin-Allophycocyanin (cross-linked) 59 

Biotin-AM3-25 15 

Biotin-B-Phycoerythrin 59 

Biotin-dATP (PCR) 55 

Biotin-dCTP (PCR) 55 

Biotin-DHPE 15 

Biotin-dUTP (PCR) 55 

Biotin-Ethylenediamine, hydrobromide 17 

Biotin-Ethylenediamine, hydrochloride 17 

Biotin-Rhodamine 110 17 

Biotin-R-Phycoerythrin 59 

Biotin-X-DHPE 15 

Biotin-XX-a-Bungarotoxin 16 

Bis-Fura-2, hexapotassium salt 20 

BLC ELISA Kit, human 93 

BLC ELISA Kit, mouse 97 

Blood DNA Isolation Buffer 29 

Blue-fluorescent Cytoplasmic Membrane Staining Kit 14 

BMP-13 (CDMP-2), human, recombinant 110 

BMP-14 (CDMP-1), human, recombinant 110 

BMP-2 EDK, human 103 

BMP-2, human, recombinant 110 


BMP-4 ELISA Kit, human 93 






BMP-7, human, recombinant (CHO) 110 

BMP-7, human, recombinant (E. coli) 110 

BNP, human, recombinant 110 

B-Phycoerythrin, lyophilized 58 

BRAK (CXCL14), human, recombinant 110 

Brefeldin A 45 

BSP ELISA Kit, human 102

146 



C-10 (CCL6), mouse, recombinant 125 

Calcein AM 14, 17 

Calcein AM Solution 14, 17 

Calcein, high purity 14, 17 

Calcium Calibration Buffer Kit 22 

Calpain Activity Assay Kit 42 

Calpain I, human (native) 42 

Calpain Inhibitor Z-LLY-FMK 42 

Calprotectin (MRP 8/14) ELISA Kit, human 102 

cAMP Activity Assay Kit 23 

Camptothecin 45 

Camptothecin (2 mM) 45 

Cardiotrophin-1 (CT-1), human, recombinant 110 

Cardiotrophin-1 (CT-1), mouse, recombinant 125 

Caspase Screening Kit 39 

Caspase-1 Colorimetric Assay Kit 41 

Caspase-1 Drug Screening Kit 39 

Caspase-1 Fluorometric Assay Kit 41 








Caspase-3 Fluorometric & Colorimetric Assay Kit 41 





Caspase-3 Fluorometric Assay Kit (HTS) 41 

Caspase-3 Immunoassay Kit 41 











Caspase-7 Immunoassay Kit 41 

Caspase-8 / FLICE ELISA Kit, human 101 





Caspase-8 Fluorometric Assay Kit (HTS) 41 



C 

Caspase-9 ELISA Kit, human 101 


Cathepsin B Activity Assay Kit 42 

Cathepsin B&L Inhibitor Z-Phe-Phe-FMK 42 

Cathepsin B, human (native) 42 

Cathepsin D Activity Assay Kit 42 

Cathepsin D, human (native) 42 

Cathepsin H Activity Assay Kit 42 

Cathepsin H, human (native) 42 

Cathepsin K Activity Assay Kit 42 

Cathepsin K ELISA Kit, human 101 

Cathepsin L Activity Assay Kit 42 

Cathepsin L ELISA Kit, human 101 

Cathepsin L, human, recombinant 42 

Cathepsin S Activity Assay Kit 42 

Cathepsin S, human (native) 42 

CD100, human, recombinant 111, 134 

CD11b, human, recombinant 110, 134 

CD14, human, recombinant (CHO) 110, 134 

CD14, mouse, recombinant (CHO) 125, 135 



CD152 (CTLA-4), human, recombinant 111, 135 

CD1A, human, recombinant 110, 134 


CD22, human, recombinant (CHO) 111, 134 



CD30 ELISA Kit, human 101 

CD30L ELISA Kit, mouse 97 

CD31 (PECAM-1), human, recombinant 111, 134 


CD4 (125-202 aa), human, recombinant 110, 134 

CD4 (203-317 aa), human, recombinant 110, 134 

CD40 ELISA Kit, human 101 

CD40L ELISA Kit, human 93, 101 

CD40L ELISA Kit, mouse 97 



CD54 (ICAM-1), human, recombinant 111, 134 






Cell Proliferation Kit I (CFSE) 8 

CellClean 83 

CFSE; 5(6)-CFDA, SE 14 

cGMP Activity Assay Kit 23 

Chk2 Inhibitor 43 

Chromogenic beta-Galactosidase Substrate Sampler Kit 12 

c-Jun (1-79)-GST Fusion Protein 43 

CNTF EDK, human 103



CNTF ELISA Kit, human 93 

CNTF, human, recombinant 111 

CNTF, rat, recombinant 129 

Coelenterazine (native) 12, 20 

Coelenterazine 400a 12, 20 

Coelenterazine cp 12, 20 

Coelenterazine f 12, 20 

Coelenterazine fcp 12, 20 

Coelenterazine h 12, 21 

Coelenterazine hcp 12, 20 

Coelenterazine i 12, 21 

Coelenterazine ip 12, 20 

Coelenterazine n 12, 21 

Coelenterazine Sampler Kit 12 

Coelenterazine, 2-methyl 12, 20 

Coelenterazinee Sampler Kit 21 

Colchicine 46 

Colorimetric Cell Viability Kit I (WST-8) 8 

Colorimetric Cell Viability Kit II (WST-1) 8 

Colorimetric Cell Viability Kit III (XTT) 8 

Colorimetric Cell Viability Kit IV (MTT) 8 

Colorimetric DNA Fragmentation Detection Kit (IHC) 33 

Colorimetric Glutathione Detection Kit 35 

Colorimetric Glutathione Reductase Activity Kit 35 

Colorimetric GST Assay Kit 25 

Colorimetric Nitric Oxide Assay Kit 25 

Competent E. coli TOP 10 cells 69 

Complete Cell Fractionation Kit 27 

CR Bacteria Test Kit 142 

CRG-2 ELISA Kit, mouse 97 

CRP ELISA Kit, human 93 

CRP, human, recombinant 111 

CTACK (CCL27), human, recombinant 111 

CTACK (CCL27), mouse, recombinant 126 

CTACK ELISA Kit, mouse 97 

CTGF (182-250 aa, His), human, recombinant 111 

CTGF (98 aa), human, recombinant 111 

CTGF (His), human, recombinant 111 

CTGF EDK, human 103 

CTGF, human, recombinant (HEK) 111 

CTGFL (WISP-2), human, recombinant 111 

Cu/ZnSOD ELISA Kit, human 101 

CXCL16 EDK, human 103 

CXCL16 ELISA Kit, mouse 97 

CXCL16, human, recombinant 111 

CXCL16, mouse, recombinant 126 

Cycloheximide 46 

Cycloheximide (100 mM) 46 

CYR61, human, recombinant 111 

Cytochrome c Apoptosis Detection Kit 34 

Cytochrome c ELISA Kit, human 101 

DAPI 13 

DAPI Solution 13 

DAPI, dilactate 13 

DASPEI 13 

Daunorubicin.HCl 46 

DcR3 ELISA Kit, human 101 

Dexamethasone 46 

Dexamethasone (10 mM) 46 

DHH, human, recombinant 111 

Di-12-ANEPPQ 17 

Di-2-ANEPEQ (JPW1114) 17 

Di-4-ANEPPS 17 

Di-8-ANEPPQ 17 

Di-8-ANEPPS 17 

DiA 15 

DiB 15 

DiBAC 4 

(3) 17 

DiD (DiIC 18 

[5]) 15 

Dihydrorhodamine 123 13 

Dihydrorhodamine 123, dihydrochloride salt 13 

DiI (or DiIC 18 

[[3]) 15 

DiI in vegetable oil 15 

DiIC1(5) 17 

Dilinoleyl DiI 15 

Dilinoleyl DiO 15 

DiO (or DiOC18[3]) 15 

DiO/DPA Membrane Potential Detection Kit 17 

DiOC 14 

(3), hydroxyethanesulfonate 15 

DiOC 16 

(3) 15 

DiOC 2 

(3) 17 

DiOC 5 

(3) 17 

DiOC 6 

(3) 13, 14, 17 

DiR (DiIC 18 

[7]) 15 

DKK-1 (Dickkopf-1) ELISA Kit, human 102 

DKK-1, human, recombinant 111 

D-Luciferin, Free Acid 12 

D-Luciferin, Potassium Salt 12 

D-Luciferin, Sodium Salt 12 

DMNPE-caged D-Luciferin 12 

DMSO, anhydrous 18, 22 

DNA Damage Detection Kit 25 

DNA Isolation Kit 29 

DNA Ladder I (100 bp) 29 

DNA Ladder II (1000 bp) 29 

Doxorubicin.HCl 46 

DPA 15 

DPA/Terbium for membrane fusion assay 17 

DPX 17 

D 

CytoRed Solution 14 

Cytosol/Particulate Fractionation Kit 27

148 



EB (ethidium bromide) Solution 13, 14 

EBI-3, human, recombinant 112 

EBI-3, mouse, recombinant 126 

EDC (EDAC) 22 

EDN (EPX) ELISA Kit, human 102 

EGF EDK, human 103 

EGF EDK, mouse 105 

EGF ELISA Kit, human 93 

EGF, human, recombinant (E. coli) 112 

EGF, human, recombinant (Pichia) 112 

EGF, mouse, recombinant 126 

EGF, rat, recombinant 129 

EGF-R (active) ELISA Kit, human 93 

EGF-R ELISA Kit, human 93 

EG-VEGF, human, recombinant 112 

EMAP-2, human, recombinant (E. coli) 112 

EMAP-2, human, recombinant (Plant) 112, 131 

ENA-78 (74 aa), human, recombinant 112 

Endostatin, human, recombinant 112 

Endothelin (1-21) ELISA Kit, human 102 

Eotaxin (CCL11) EDK, human 103 

Eotaxin (CCL11), human, recombinant 112 

Eotaxin (CCL11), mouse, recombinant 126 

Eotaxin ELISA Kit, mouse 97 

Eotaxin-2 (CCL24), human, recombinant 112 

Eotaxin-2 (CCL24), mouse, recombinant 126 

Eotaxin-2 ELISA Kit, human 93 

Eotaxin-3 (CCL26), human, recombinant 112 

Eotaxin-3 EDK, human 103 

Epigen, human, recombinant 112 

Epiregulin, human, recombinant 112 

EPO ELISA Kit, human 93 

EPO-alpha, human, recombinant 112 

E-selectin (CD62E), human, recombinant 112, 134 

Ethidium Homodimer I 13 

Ethidium Homodimer III 13 

Etoposide 46 

Etoposide (10 mM) 46 

Exodus-2 (CCL21), mouse, recombinant 126 

Exodus-2, human, recombinant 112 

E 

FGF-16, human, recombinant 113 




FGF-2 (FGF-b) EDK, human 103 

FGF-2 (FGF-b), human, recombinant (E. coli) 112 

FGF-2 (FGF-b), human, recombinant (Plant) 112, 131 

FGF-2 (FGF-b), human, recombinant (Sf9) 112 

FGF-2 (FGF-b), mouse, recombinant 126 

FGF-2 (FGF-b), rat, recombinant 129 

FGF-2 (FGF-b, 147 aa), human, recombinant 112 

FGF-2 / FGF-b ELISA Kit, human 93 



FGF-21, mouse, recombinant 126 



FGF-4 ELISA Kit, human 93 







FGF-9, human, recombinant (E. coli) 113 

FGF-9, human, recombinant (Sf9) 113 

FGF-9, mouse, recombinant 126 

FGF-9, rat, recombinant 129 

Fibronectin ELISA Kit, human 102 

FIP-18 AM 20 

flt-3 Ligand ELISA Kit, human 94 

flt-3 Ligand, human, recombinant (E. coli) 113 

flt-3 Ligand, human, recombinant (Plant) 113, 131 

flt-3 Ligand, human, recombinant (Sf9) 113 

flt-3 Ligand, mouse, recombinant 126 

Flubi-2 22 

Flubida-2 22 

Fluo-3 (pentaammonium salt) 19 

Fluo-3 (pentapotassium salt) 19 

Fluo-3 (pentasodium salt) 19 

Fluo-3 AM 19 

Fluo-3 AM Solution 19 

FluoDNA Gel Stain 29 

Fas ELISA Kit, human 93 

FFP-18 AM 20 

FGF-1 (FGF-a), human, recombinant (E. coli) 112 

FGF-1 (FGF-a), human, recombinant (Plant) 112, 131 

FGF-1 (FGF-a), human, recombinant (Sf9) 112 

FGF-1 (FGF-a), mouse, recombinant 126 



F 

Fluorescein-a-bungarotoxin 16 

Fluorescein-Biotin 18 

Fluorescein-DHPE 15 

Fluorometric Cell Viability Kit I (Resazurin) 8 

Fluorometric Cell Viability Kit II (Calcein) 8 

Fluorometric DNA Fragmentation Detection Kit I (BrdU) 33 

Fluorometric DNA Fragmentation Detection Kit II (BrdU) 33 

Fluorometric DNA Fragmentation Detection Kit III (F-dUTP) 33 

Fluorometric Glutathione Detection Kit 35 

Fluorometric GST Assay Kit 25



Fluorometric Nitric Oxide Assay Kit 25 

Follistatin EDK, human 103 

Fractalkine (CX3CL1), human, recombinant 113 

Fractalkine (CX3CL1), rat, recombinant 129 

Fractalkine ELISA Kit, human 94 

Fractalkine ELISA Kit, rat 99 

FST, human, recombinant 113 

Fura-2 (pentaammonium salt) 19 

Fura-2 (pentapotassium salt) 19 

Fura-2 (pentasodium salt) 19 

Fura-2 AM 19 

Fura-2 AM Solution 19 

Fura-2FF (K + Salt) 20 

Fura-2FF AM 20 

Furaptra, AM ester 20 

Furaptra, tetrapotassium salt 20 

Furaptra, tetrasodium salt 20 

GeneOFF pPK-U6-GFP shRNA Vector Kit 70 

GeneOFF siRNA Generation Kit 71 

Genistein 46 

Genomic DNA Isolation Kit I 29 

Genomic DNA Isolation Kit II 29 

GFP Reporter Assay Kit 12 

GH-20K-pit, human, recombinant 114 

GH-20K-pl, human, recombinant 114 

GH-22K-pl, human, recombinant 114 

GHBP, human, recombinant 114 

GITR ELISA Kit, human 94 

GM-CSF EDK, human 103 

GM-CSF EDK, mouse 105 

GM-CSF ELISA Kit, human 94 

GM-CSF ELISA Kit, mouse 97 

GM-CSF ELISA Kit, rat 99 

GM-CSF, human, recombinant (E. coli) 114 

GM-CSF, human, recombinant (Plant) 114, 132 

G 

gAcrp30 (Adipolean), human, recombinant 109 

gAcrp30, mouse, recombinant 125 

gAcrp30/Adipolean Variant, human, recombinant 109 

Galectin-1, human, recombinant 113 

Galectin-3 ELISA Kit, human 94, 102 



GCP-2 (CXCL6), human, recombinant 113 

G-CSF EDK, human 103, 105 

G-CSF ELISA Kit, human 94, 97 

G-CSF, human, recombinant (CHO) 113 

G-CSF, human, recombinant (E. coli) 113 

G-CSF, human, recombinant (Plant) 113, 131 

G-CSF, mouse, recombinant 126 

GDF-11, human, recombinant 114 

GDF-15 (MIC-1), human, recombinant 114 





GDNF, human, recombinant (E. coli) 114 

GDNF, human, recombinant (Plant) 114, 132 

GDNF, mouse, recombinant 126 

GDNF, rat, recombinant 129 

GelQuick Protein Gel Stain/Destaining Kit 27 

GeneBooster Reagent I 83 

GeneBooster Reagent II 83 

GeneBooster Reagent III 83 

GeneBooster Reagent Kit 83 

GeneOFF pPK-H1 shRNA Vector Kit 70 

GeneOFF pPK-H1-GFP shRNA Vector Kit 70 

GeneOFF pPK-U6 shRNA Vector Kit 70 

GM-CSF, human, recombinant (Sf9) 114 

GM-CSF, mouse, recombinant (E. coli) 126 

GM-CSF, mouse, recombinant (Plant) 126, 133 

GM-CSF, rat, recombinant 129 

GM-CSF/IL-3 FP, human, recombinant (E. coli) 114 

GMF-beta, human, recombinant 114 

GMF-gamma, human, recombinant 114 

Granzyme A ELISA Kit, human 101 

Granzyme B ELISA Kit, human 102 

Granzyme B, mouse, recombinant 126 

Green Caspase-12 Staining Kit 38 





Green Multi-Caspase Staining Kit 38 

Green-beta-D-Gal 12 

Green-fluorescent Cytoplasmic Membrane Staining Kit 14 

GRO (KC, CXCL1) EDK, rat 106 

GRO-alpha (KC, CXCL1), rat, recombinant 129 

GRO-alpha (MGSA, CXCL1) EDK, human 104 

GRO-alpha (MGSA, CXCL1), human, recombinant 114 

GRO-beta (MIP-2, CXCL2) EDK, human 104 

GRO-beta (MIP-2, CXCL2), human, recombinant 114 

GRO-beta (MIP-2, CXCL2), rat, recombinant 129 

GRO-gamma (CXCL3), human, recombinant 114 

Growth Hormone (GH), human, recombinant 114 

Growth Hormone (GH), mouse, recombinant 126 

Growth Hormone (GH), rat, recombinant 129 

GSK-3a-GST Fusion Protein 43 

GSK-3β, human, recombinant 43 

GST Inhibitor-1 (Cibacron Blue F3G-A) 25 

GST Inhibitor-2 (ethacrynic acid) 25 

GST protein (active) 25

150 



HAT Activity Assay Kit 25 

HAT protein (P/CAF, active) 25 

HB-EGF, human, recombinant (Plant) 114, 132 

HCC-1 (66 aa, CCL14), human, recombinant 114 

HCC-1 (72 aa, CCL14), human, recombinant 114 

HCC-4 ELISA Kit, human 94 

HDGF, human, recombinant 114 

Heregulin beta-1, human, recombinant 114 

HGF ELISA Kit, human 94 

HGF, human, recombinant (CHO) 114 

HGF, human, recombinant (Sf9) 114 

HGF-A, human, recombinant 115 

HGF-B, human, recombinant 115 

Hoechst 33258 13 

Hoechst 33258 Solution 13,14 

Hoechst 33342 13,14 

Hoechst 33342 Solution 13,14 

HVEM/Fc, human, recombinant 115 

Hydroxystilbamidine 18 

I-309 (CCL1), human, recombinant 115 

IFN-alpha 1, human, recombinant (E. coli) 115 

IFN-alpha 1b, human, recombinant (E. coli) 115 

IFN-alpha 2a, human, recombinant (E. coli) 115 

IFN-alpha 2a, human, recombinant (Plant) 115, 132 

IFN-alpha 2b, human, recombinant (E. coli) 115 

IFN-alpha 2b, human, recombinant (Yeast) 115 

IFN-alpha ELISA Kit, human 94 

IFN-beta 1a, human, recombinant (CHO) 115 

IFN-beta 1b, human, recombinant (E. coli) 115 

IFN-gamma EDK, human 104 

IFN-gamma EDK, mouse 105 

IFN-gamma EDK, rat 106 

IFN-gamma ELISA Kit, human 94 

IFN-gamma ELISA Kit, mouse 97 

IFN-gamma ELISA Kit, pig 100 

IFN-gamma ELISA Kit, rat 99 

IFN-gamma, human, recombinant (E. coli) 115 

IFN-gamma, human, recombinant (Plant) 115, 132 

IFN-gamma, mouse, recombinant 126 

IFN-gamma, rat, recombinant 129 

IFN-lambda-1 (IL-29), human, recombinant 115 

IFN-lambda-2 (IL-28A), human, recombinant 115 

IFN-lambda-2 (IL28A), mouse, recombinant 126 

IFN-omega ELISA Kit, human 94 

IFN-omega, human, recombinant 115 

IGF-BP1 ELISA Kit, human 94 

IGF-BP1, human, recombinant 115 



H 

I 






IGF-BP6 ELISA Kit, mouse 97 

IGF-BP6, human, recombinant (E. coli) 116 

IGF-BP6, human, recombinant (InC) 116 


IGF-I EDK, mouse 105 

IGF-I ELISA Kit, human 94 

IGF-I, human, recombinant (E. coli) 115 

IGF-I, human, recombinant (Plant) 115, 132 

IGF-I, mouse, recombinant 126 

IGF-I, rat, recombinant 129 

IGF-II, human, recombinant 115 

IGF-IR, human, recombinant 115 

IL-1 alpha EDK, human 104 

IL-1 alpha EDK, mouse 105 

IL-1 alpha EDK, rat 106 

IL-1 alpha ELISA Kit, human 94 

IL-1 alpha ELISA Kit, mouse 97 

IL-1 alpha ELISA Kit, rat 99 

IL-1 alpha, human, recombinant (E. coli) 116 

IL-1 alpha, human, recombinant (Plant) 116, 132 

IL-1 alpha, mouse, recombinant 126 

IL-1 alpha, rat, recombinant 129 

IL-1 beta EDK, mouse 105 

IL-1 beta EDK, rat 106 

IL-1 beta ELISA Kit, human 94 

IL-1 beta ELISA Kit, monkey 99 

IL-1 beta ELISA Kit, mouse 97 

IL-1 beta ELISA Kit, rat 99 

IL-1 beta, human, recombinant 116 

IL-1 beta, mouse, recombinant 126 

IL-1 beta, rat, recombinant 129 

IL-1 RA ELISA Kit, human 94 

IL-1 RA, human, recombinant 116 

IL-10 EDK, human 104 

IL-10 EDK, mouse 105 

IL-10 ELISA Kit, human 95 

IL-10 ELISA Kit, monkey 99 

IL-10 ELISA Kit, mouse 98 

IL-10 ELISA Kit, rat 99 

IL-10, human, recombinant (CHO) 117 

IL-10, human, recombinant (E. coli) 117 

IL-10, mouse, recombinant 126 

IL-10, rat, recombinant 130 


IL-11, human, recombinant 117 


IL-12 (p40 + p70) ELISA Kit, mouse 98 

IL-12 (p40, IL-23) ELISA Kit, human 95 

IL-12 (p70) ELISA Kit, monkey 99



IL-12 (p70) ELISA Kit, human 95 

IL-12 (p70) ELISA Kit, mouse 98 




IL-12, mouse, recombinant (CHO) 126 


IL-12-p40, human, recombinant (CHO) 117 

IL-12-p80, human, recombinant (InC) 117 

IL-13 (109 aa), rat, recombinant 130 

IL-13 (113 aa), rat, recombinant 130 

IL-13 (variant), human, recombinant 117 











IL-16 (121 aa), human, recombinant 117 

IL-16 (130 aa), human, recombinant 117 

IL-16, human, recombinant (Plant) 117, 132 







IL-17A EDK, human 104 

IL-17A/F, human, recombinant 117 

IL-17A/F, mouse, recombinant 127 

IL-17B, human, recombinant 117 

IL-17D, human, recombinant 117 

IL-17E (IL-25), rat, recombinant 130 

IL-17E (IL-25, SF20), human, recombinant (E. coli) 117 

IL-17E (IL-25, SF20), human, recombinant (Plant) 117, 132 

IL-17E (IL-25, SF20), mouse, recombinant 127 

IL-17E EDK, human 104 

IL-17F EDK, human 104 

IL-17F, human, recombinant 117 

IL-17F, mouse, recombinant 127 

IL-17F, rat, recombinant 130 


IL-18 ELISA Kit, pig 100 




IL-1RL1, human, recombinant 116 









IL-2, human, recombinant (Yeast) 116 




















IL-25 (SF20), mouse, recombinant 127 

IL-27 (p28), mouse, recombinant 127 

IL-2R ELISA Kit, human 94 

IL-3 beta, rat, recombinant 129 

IL-3 CC, human, recombinant 116 





IL-3, human, recombinant (Sf9) 116 








IL-32 alpha, human, recombinant 117 











IL-4, human, recombinant (CHO) 116

152 











IL-5, human, recombinant (Plant) 132 







IL-6 EDK, rat 106 

Insulin, human, recombinant (Yeast) 117 

Ionomycin (calcium salt) 22, 46 

Ionomycin (free acid) 22, 46 

IP-10 (CXCL10), human, recombinant 117 

IP-10 (CXCL10), mouse, recombinant 127 

IP-10 (CXCL10), rat, recombinant 130 

IP-10 EDK, human 104 

IP-10 ELISA Kit, human 95 

IP-10 ELISA Kit, mouse 98 

IRF-1, human, recombinant 117 




I-TAC (CXCL11), human, recombinant 118 

I-TAC (CXCL11), mouse, recombinant 127 

I-TAC EDK, human 104 




IL-6, human, recombinant (CHO) 116 





IL-6R ELISA Kit, human 94 

JC-1 chloride 13 

JC-1 iodide 14 

JNK Activated Cell Lysate 43 

JNK Activity Detection Kit 43 

JNK Activity Screening Kit 43 

JNK Negative Control Cell Lysate 43 

J 










IL-8 (CXCL8), human, recombinant (Pichia) 116 

IL-8 (CXCL8, 72 aa), human, recombinant 116 

IL-8 (CXCL8, 77 aa), human, recombinant 117 









Immunglobulin E [IgE] ELISA Kit, human 102 

Immunglobulin G [IgG] ELISA Kit, human 102 

Indo-1 (pentaammonium salt) 19 

Indo-1 (pentapotassium salt) 19 

Indo-1 (pentasodium salt) 19 

Indo-1 AM 19 

Indo-1FF (K + Salt) 20 

Indo-1FF AM 20 

Insulin, human, recombinant (E. coli) 117 

KC (GRO-alpha, CXCL1) EDK, mouse 105 

KC (GRO-alpha, CXCL1), mouse, recombinant 127 

KGF (FGF-7), human, recombinant (E. coli) 118 

KGF (FGF-7), human, recombinant (Plant) 118, 132 

KGF-2, human, recombinant 118 

Klotho, human, recombinant 118 

LAG-1 (CCL4L1), human, recombinant 118 

LD78 beta (CCL3L1), human, recombinant 118 

LDH Cytotoxicity Kit II 10 

LEC (CCL16, NCC-4), human, recombinant 118 

Leptin EDK, human 104 

Leptin EDK, mouse 105 

Leptin ELISA Kit, human 95 

Leptin ELISA Kit, mouse 98 

Leptin Receptor, human, recombinant 118 

Leptin, human, recombinant 118 

Leptin, mouse, recombinant 127 

Leptin, rat, recombinant 130 

LeukinFeron, human, native 118 

LFA-3 (CD58), human, recombinant 118, 134 

LIF ELISA Kit, human 95 

LIF, human, recombinant (E. coli) 118 

LIF, human, recombinant (Plant) 118, 132 

K 

L



LIF, mouse, recombinant (E. coli) 127 

LIF, mouse, recombinant (Plant) 127, 133 

LIF-R (gp190) ELISA Kit, human 95 

Light ELISA Kit, human 95 

LIGHT, human, recombinant 118 

LIGHT, mouse, recombinant 127 

Live/Dead Cell Staining Kit II 9 

LIX (CXCL5, 70 aa), mouse, recombinant 127 

LIX (CXCL5, 93 aa), mouse, recombinant 127 

LIX ELISA Kit, rat 99 

L-JNK Peptide Inhibitor I 43 

L-JNKI1 Negative Control Peptide 43 

L-selectin (CD62L), human, recombinant 118, 134 

Lucifer Yellow Cadaverine 18 

Lucifer Yellow CH lithium salt 18 

Lucifer Yellow CH potassium salt 18 

Luciferase Reporter Assay Kit I (Firefly) 11 

Luciferase Reporter Assay Kit II (Renilla) 11 

Luciferase Reporter Assay Kit III (Firefly & Renilla) 11 

Luciferase Reporter HTS Kit (Firefly) 11 

Lungkine (CXCL15), mouse, recombinant 127 

Lymphotactin, human, recombinant 118 

M 

MA Lipofection Enhancer 82 

Magnet Plate 82 

Magnetic Separator (re-usable) 45 

Mammalian Whole Cell Protein Extraction Kit 27 

Maspin, human, recombinant 118 

MATra-A Reagent 82 

MATra-S Immobilizer 82 

MATra-siRNA Reagent 82 

MCP-1 (CCL2) EDK, rat 106 

MCP-1 (CCL2), rat, recombinant 130 

MCP-1 (JE, CCL2) EDK, mouse 105 

MCP-1 (JE, CCL2), mouse, recombinant 127 

MCP-1 (MCAF, CCL2) EDK, human 104 

MCP-1 (MCAF, CCL2), human, recombinant 118 

MCP-1 ELISA Kit, human 95 

MCP-1 ELISA Kit, mouse 98 

MCP-1 ELISA Kit, rat 99 

MCP-2 (CCL8), human, recombinant 118 

MCP-2 (CCL8), mouse, recombinant 127 

MCP-2 ELISA Kit, human 95 

MCP-3 (CCL7) EDK, mouse 106 





MCP-5 ELISA Kit, mouse 98 

M-CSF EDK, mouse 106 

M-CSF ELISA Kit, human 95 

M-CSF, human, recombinant (E. coli) 118 

M-CSF, human, recombinant (Plant) 118, 132 

M-CSF, mouse, recombinant 127 

M-CSF, rat, recombinant 130 

MDC (CCL22) EDK, mouse 106 

MDC (CCL22), mouse, recombinant 127 

MDC (CCL22, 67 aa), human, recombinant 119 

MDC (CCL22, 69 aa), human, recombinant 119 

MDC ELISA Kit, human 95 

MEC (CCL28), human, recombinant 119 

MEC (CCL28), mouse, recombinant 128 

Membrane Protein Extraction Kit 27 

MIA, human, recombinant 119 

MIA-2 EDK, human 104 

MIA-2, human, recombinant 119 

Midkine (MK) EDK, human 104 

Midkine, human, recombinant 119 

MIF (active), human, recombinant 119 

MIF, human, recombinant 119 

MIG (CXCL9), human, recombinant 119 

MIG (CXCL9), mouse, recombinant 128 

MIG ELISA Kit, human 95 

MIP-1 alpha (CCL3) EDK, human 104 

MIP-1 alpha (CCL3) EDK, mouse 106 

MIP-1 alpha (CCL3), human, recombinant (E. coli) 119 

MIP-1 alpha (CCL3), human, recombinant (Plant) 119, 132 

MIP-1 alpha (CCL3), mouse, recombinant 128 

MIP-1 alpha (CCL3), rat, recombinant 130 

MIP-1 alpha (CCL4) EDK, rat 106 

MIP-1 alpha ELISA Kit, mouse 98 

MIP-1 beta (CCL4), human, recombinant 119 

MIP-1 beta (CCL4), mouse, recombinant 128 

MIP-1 beta (CCL4), rat, recombinant 130 

MIP-1 beta ELISA Kit, human 95 

MIP-1 delta ELISA Kit, human 95 

MIP-1 gamma (CCL9/10), mouse, recombinant 128 

MIP-2 (GRO-beta, CXCL2) EDK, mouse 106 

MIP-2 (GRO-beta, CXCL2), mouse, recombinant 128 

MIP-2 ELISA Kit, mouse 98 

MIP-3 (CCL23), human, recombinant 119 

MIP-3 alpha (CCL20), human, recombinant 119 

MIP-3 alpha (CCL20), mouse, recombinant 128 

MIP-3 alpha ELISA Kit, human 95 

MIP-3 beta (CCL19), human, recombinant 119 

MIP-3 beta (CCL19), mouse, recombinant 128 



Mitochondria/Cytosol Fractionation Kit 27 

Mitochondrial Apoptosis Staining Kit 34 

Mitochondrial DNA Isolation Kit 29 

Mitomycin C 46 

MMP-1 ELISA Kit, human 95 

MMP-1, human, recombinant 119 


MMP-13 ELISA Kit, human 96

154 










NT-3 ELISA Kit, human 96 

NT-3, human, recombinant 120 

NT-3, mouse, recombinant 128 

NT-4, human, recombinant 120 

NT-proANP 1-98 ELISA Kit, human 102 

NT-proCNP ELISA Kit, human 102 

Nuclear/Cytosol Fractionation Kit 27 



MPO ELISA Kit, human 96 

MQAE 20 

MSP ELISA Kit, human 96 

Mycoplasma Disinfection Kit 143 

Mycoplasma-EX 141 

Mycoplasma-EX Spray 143 

Myostatin ELISA Kit, human 96 

Myostatin Propetide, human, recombinant 119 

Myostatin, human, recombinant 119 

Octadecyl Rhodamine B Chloride (R18) 16 

Okadaic acid 46 

Omentin, human, recombinant 120 

Oncostatin M (209 aa), human, recombinant 120 

Oncostatin M (227 aa), human, recombinant 120 

Oncostatin M, rat, recombinant 130 

OPG (OCIF), human, recombinant 120 

OPG ELISA Kit, human 96, 102 

O 

Orange-fluorescent Cytoplasmic Membrane Staining Kit 14 

NAD/NADH Quantitation Kit 25 

NADP/NADPH Quantitation Kit 25 

Nanog, human, recombinant 119 

NAP-2 (CXCL7) EDK, human 104 

NAP-2 (CXCL7), human, recombinant 119 

NAP-2 ELISA Kit, human 96 

N 

Osteonectin (ON) ELISA Kit, human 102 

Osteopontin (SPP-1), human, recombinant 120 

OSTF-1, human, recombinant 120 

OTOR, human, recombinant 120 

OX-40 / CD134 ELISA Kit, human 96, 100, 102 

OX40L, human, recombinant 120 

Oxidized Glutathione (GSSG) 25 

Oxidized LDL (oxLDL) ELISA Kit, human 102 

NBD C6-ceramide 15 

NBD C6-sphingomyelin 16 

NBD-DHPE 15 

Nerve Terminal Green Staining Kit I 16 

Nerve Terminal Green Staining Kit II 16 

Nerve Terminal Green Staining Kit III 16 

Nerve Terminal Green Staining Kit IV 16 

Nerve Terminal Red Staining Kit I 16 

Nerve Terminal Red Staining Kit II 16 

Neuregulin-1 (NRG-1), human, recombinant (E. coli) 120 

Neuregulin-1 (NRG-1), human, recombinant (Plant) 120, 132 

Neuritin, human, recombinant 120 

Neuro-DiI 15 

Neuro-DiO 15 

Neuroglobin, human, recombinant 120 

Neuropoietin (NP), mouse, recombinant 128 

Neuroserpin, human, recombinant 120 

Neurturin (NTN), human, recombinant 120 

NNT-1 (BCSF-3), human, recombinant 120 

NOGGIN, human, recombinant (E. coli) 120 

NOGGIN, human, recombinant (HEK) 120 

NOGGIN, mouse, recombinant 128 

Nonyl Acridine Orange (or NAO) 14 

NOV EDK, human 104 

NOV, human, recombinant 120 

NP-1, human, recombinant 120 

P 

p185 (HER-2) ELISA Kit, human 96 

p53 ELISA Kit, human 103 

PAF-AH, human, recombinant 120 

PAI-1 EDK, human 104 

PAI-1 ELISA Kit, human 96 

PAI-2, human, recombinant 120 

PARC ELISA Kit, human 96 

PCR Mycoplasma Test Kit I/C 140 

PCR Mycoplasma Test Kit I/RT; Variant A 140 

PCR Mycoplasma Test Kit I/RT; Variant B 140 

PCR Mycoplasma Test Kit III 140 

PCR Purification Kit 29 

PDGF-AA, human, recombinant (E. coli) 120 

PDGF-AA, human, recombinant (Pichia) 120 

PDGF-AA, mouse, recombinant 128 

PDGF-AB, human, recombinant (E. coli) 120 

PDGF-BB EDK, human 105 

PDGF-BB ELISA Kit, human 96 

PDGF-BB, human, recombinant (E. coli) 120 

PDGF-BB, human, recombinant (Plant) 120, 132 

PDGF-BB, human, recombinant (Yeast) 121 

PDGF-BB, mouse, recombinant 128 

PDGF-CC, human, recombinant 121 

PEDF, human, recombinant (E. coli) 121



PEDF, human, recombinant (HEK) 121 

Periostin, human, recombinant 121 

Persephin, human, recombinant 121 

Persephin, mouse, recombinant 128 

PF XXX-Goat Anti-Mouse IgG 54 

PF-4 (CXCL4), human, recombinant 121 

PF-4 (CXCL4), mouse, recombinant 128 

PF-XXX-aadUTP 55 

PF-XXX-Biotin Conjugate 56 

PF-XXX-Goat Anti-Human IgG 54 

PF-XXX-Goat Anti-Rabbit IgG 54 

PF-XXX-Labeling-Kit 52 

PF-XXX-Phalloidin 54 

PF-XXX-Streptavidin Conjugate 56 

PGRN, human, recombinant 121 

Pharmacidal Spray 143 

Phorbol-12-myristate 13-acetate (PMA) 46 

Plant DNA Isolation Buffer 29 

Pleiotrophin, human, recombinant 121 

PLGF-1 EDK, human 105 

PLGF-1, human, recombinant (E. coli) 121 

PLGF-1, human, recombinant (Sf9) 121 

PLGF-2, human, recombinant (E. coli) 121 

PLGF-2, human, recombinant (Sf9) 121 

PLGF-2, mouse, recombinant (E. coli) 128 

PLGF-3, human, recombinant 121 

Pluronic F-127 18, 22 

Pluronic F-127, 10% in H 2 

O 18, 22 

Pluronic F-127, 20% solution in DMSO 18, 22 

PMN-Elastase ELISA Kit, human 96 

pPK-CMV-E1 Expression Vector Kit 63 



pPK-CMV-F1 Fusion Vector (C-GFP) 65 

pPK-CMV-F2 Fusion Vector (N-GFP) 65 

pPK-CMV-F3 Fusion Vector (C-Luc) 65 

pPK-CMV-F4 Fusion Vector (N-Luc) 65 

pPK-CMV-R0 Reporter Vector (Blank) 64 

pPK-CMV-R1 Reporter Vector (β-Gal) 64 

pPK-CMV-R2 Reporter Vector (CAT) 64 

pPK-CMV-R3 Reporter Vector (Luc) 64 

PromoFectin-CellLine 78 

PromoFectin-Hepatocyte 78 

PromoFectin-HUVEC 78 

PromoFectin-Insect 78 

PromoFectin-Macrophage 78 

PromoFectin-Neuron 78 

PromoFectin-Polypeptide 78 

PromoFectin-siRNA 78 

PromoFluor Amino-Modified Label 51 

PromoFluor Antifade Reagent 60 

PromoFluor Carboxylic Acid 51 

PromoFluor ChipPack 51 

PromoFluor Maleimide 51 

PromoFluor NHS-Ester 51 

PromoFluor-430 Nick Translation Labeling Kit 53 

PromoFluor-430 PCR Labeling Kit 53 

PromoFluor-430-dUTP (NT) 55 

PromoFluor-430-dUTP (PCR) 55 

















Pro-NGF, human, recombinant 121 

Propidium Iodide (PI) 13, 14 

Propidium Iodide (PI) Solution 13, 14 

Prox-1 (fragment), human, recombinant 121 

P-selectin (CD62P), human, recombinant 121, 134 

PTHrP, human, recombinant 121 

Purple-beta-D-Gal 12 

pPK-CMV-R4 Reporter Vector (GFP) 64 

pPK-CMV-R5 Reporter Vector (SEAP) 64 

Prestained Protein Ladder I 27 

Prestained Protein Ladder II 27 

ProBMP-2, human, recombinant 121 

Prokinectin-2, human, recombinant 121 

Prolactin Receptor (Prlr), human, recombinant 121 

Prolactin Receptor (Prlr), rat, recombinant 130 

Prolactin, human, recombinant (E. coli) 121 

Prolactin, human, recombinant (Plant) 121, 132 

Prolactin, mouse, recombinant 128 

Prolactin, rat, recombinant 130 

PromoFectin 78 

R 

RANTES (CCL5) EDK, human 105 

RANTES (CCL5) EDK, mouse 106 

RANTES (CCL5) EDK, rat 106 

RANTES (CCL5), human, recombinant 121 

RANTES (CCL5), mouse, recombinant 128 

RANTES (CCL5), rat, recombinant 130 

RANTES ELISA Kit, human 96 

Rapamycin 46 

RBC Lysis Solution 29 

RBP ELISA Kit, human 103

156 



RBP-4, human, recombinant 121 

Red Caspase-3 Staining Kit 38 



Red Multi-Caspase Staining Kit 38 

Red-beta-D-Gal 12 

Red-fluorescent Cytoplasmic Membrane Staining Kit 14 

Reduced Glutathione (GSSG) 25 

Relaxin ELISA Kit, human 101 

Relaxin-2, human, recombinant 121 

Relaxin-3, human, recombinant 121 

RELM beta, human, recombinant 122 

RELM-alpha, mouse, recombinant 128 

RELM-beta, mouse, recombinant 128 

RELM-gamma, mouse, recombinant 128 

Resazurin, sodium salt 8 

Resistin EDK, human 105 

Resistin ELISA Kit, human 96 

Resistin, human, recombinant 122 

Resistin, mouse, recombinant 128 

Resistin, rat, recombinant 130 

RH237 17 

RH414 17 

RH421 17 

RH795 17 

Rhod-2 (triammonium salt) 19 

Rhod-2 (tripotassium salt) 20 

Rhod-2 (trisodium salt) 20 

Rhod-2 AM 20 

Rhodamine 123 14 

Rhodamine-DHPE 15 

RNA Isolation Kit I 29 

RNA Isolation Kit II 29 

Rose-beta-D-Gal 12 

Rosiglitazone 46 

R-Phycoerythrin, lyophilized 58 

RuBP-2S 18 

RuBP-4S 18 

RuBPS 18 

sCD40 Ligand (CD154), human, recombinant 111, 134 

sCD40 Ligand, mouse, recombinant 125, 135 

sCD40L EDK, human 103 

sCD44std ELISA Kit, human 100 

sCD44var (v5) ELISA Kit, human 100 

sCD44var (v6) ELISA Kit, human 100 

sCD8 ELISA Kit, human 100 

SCF EDK, human 105 

SCF EDK, mouse 106 

SCF EDK, rat 106 

SCF ELISA Kit, human 96 

SCF ELISA Kit, mouse 98 

SCF, human, recombinant (E. coli) 122 

SCF, human, recombinant (Sf9) 122 

SCF, mouse, recombinant (E. coli) 128 

SCF, mouse, recombinant (Plant) 128, 133 

SCF, rat, recombinant 130 

SCGF-alpha, human, recombinant 122 

SCGF-beta, human, recombinant 122 

sCTLA-4 / CD152 ELISA Kit, human 100 

SDF-1 alpha (CXCL12), human, recombinant 122 

SDF-1 alpha (CXCL12), mouse, recombinant 128 

SDF-1 alpha (CXCL12), rat, recombinant 130 

SDF-1 alpha ELISA Kit, human 96 

SDF-1 alpha ELISA Kit, mouse 98 

SDF-1 beta (CXCL12), human, recombinant 122 

SDF-1 beta (CXCL12), mouse, recombinant 128 

SDF-1 beta (CXCL12), rat, recombinant 130 

SDIP/Europium for membrane fusion assay 18 

sDLL-1, human, recombinant 112 

sDLL-4, human, recombinant 112 

sEGF-R, human, recombinant 112 

Senescence Detection Kit 10 

sE-selectin / CD62E ELISA Kit, monkey 99, 101 

sE-selectin / CD62E ELISA Kit, human 93, 100 

Set of four Magnet Bar Plates 82 

sFas Ligand ELISA Kit, human 102 

sFas Ligand, human, recombinant 112 

sFas Receptor (CD95), human, recombinant 112, 134 

sFGFR-1/Fc, human, recombinant (Sf9) 113 

sBMPR-1A, human, recombinant (Sf9) 110 

SCAS 16 

sCD105 (Endoglin), human, recombinant (E. coli) 111, 134 

sCD105 (Endoglin), human, recombinant (Sf9) 111, 134 

sCD105 (Endoglin), mouse, recombinant (Sf9) 125, 135 

sCD153 / sCD30L ELISA Kit, human 100 





sCD4, human, recombinant 110, 134 


S 




sFRP-1, human, recombinant 113 

sgp130 ELISA Kit, human 96 

Shh, human, recombinant (E. coli) 122 

Shh, mouse, recombinant 128 

sICAM-1 / CD54 ELISA Kit, human 94, 100 

sICAM-1 / CD54 ELISA Kit, monkey 99, 101 



sIL-2R alpha (CD25), human, recombinant 116, 134 

sIL-4R alpha (CD124), human, recombinant 116, 135 

sIL-6 Receptor (CD126), human, recombinant 116, 135



siProtect RNase Inhibitor 71 

Slit2-N, human, recombinant 122 

sL-Selectin ELISA Kit, human 95 

sLYVE-1, human, recombinant (Sf9) 118 

sLYVE-1, mouse, recombinant (Sf9) 127 

SMCC-activated Allophycocyanin, lyophilized 58 

SMCC-activated R-Phycoerythrin, lyophilized 58 

SOD Assay Kit 25 

sPECAM-1 / CD31 ELISA Kit, human 96, 100 

sPECAM-1 / CD31 ELISA Kit, monkey 99,101 

SPQ 21 

sP-selectin / CD62P ELISA Kit, human 96, 100 

sP-selectin / CD62P ELISA Kit, mouse 98, 100 

sPSGL-1 / CD162 ELISA Kit, human 96, 100 

sRANK Receptor, human, recombinant 121 

sVEGFR-1 (flt-1, D1-4) human, recombinant (Sf9) 124 


sVEGFR-1 (flt-1, D7-Fc) human, recombinant (Sf9) 124 

sVEGFR-1 (flt-1, native), human, recombinant (Sf9) 124, 125 

sVEGFR-2 (sKDR, D1-D7) human, recombinant (Sf9) 125 

sVEGFR-2 (sKDR-Fc, D7) human, recombinant (Sf9) 125 

sVEGFR-3 (flt-4), human, recombinant (Sf9) 125 

SynapseGreen C1 16 

SynapseGreen C18 (FM3-25) 16 


SynapseGreen C3 16 



SynapseRed C2 (FM4-64) 16 

SynapseRed C2M (FM5-95) 16 

sRANKL (total) ELISA Kit, human 96 

sRANKL EDK, human 105 

sRANKL EDK, mouse 106 

sRANKL ELISA Kit, human 96 

sRANKL, human, recombinant (E. coli) 121 

sRANKL, human, recombinant (Plant) 121, 132 

sRANKL, mouse, recombinant 128 

sRANKL, rat, recombinant 130 

StarGate Combi Entry Cloning Set 69 

StarGate Combi Newcomer Set 69 

StarGate Fusion Cloning Set "IRES1" 69 

StarGate Fusion Cloning Set "LINK1" 69 

StarGate Fusion Cloning Set "SD1" 69 

StarGate Transfer Reagent Set 69 

Staurosporine 46 

sTIE-1 / Fc Chimera, mouse, recombinant (Sf9) 128 

sTIE-1/Fc Chimera, human, recombinant (Sf9) 122 

sTIE-2 / Fc Chimera, mouse, recombinant (Sf9) 128 

sTIE-2/Fc Chimera, human, recombinant (Sf9) 122 

sTNF-R1 (CD120a), human, recombinant 123, 134 

sTNF-R2 (CD120b), human, recombinant 123, 134 

sTRAIL (APO2L) EDK, human 105 

sTRAIL Receptor-1 (DR4), human, recombinant 123 

sTRAIL Receptor-2 (DR5), human, recombinant 123 

Streptavidin-Allophycocyanin 59 

Streptavidin-B-Phycoerythrin 59 

Streptavidin-Fluorescein-5-isothiocyanate (FITC) 59 

Streptavidin-R-Phycoerythrin 59 

Sulforhodamine 101 18 

Sulforhodamine 101-a-bungarotoxin 16 

Sulforhodamine B 18 

Sulforhodamine G 18 

Superoxide Dismutase (SOD, human) 25 

sVCAM-1 / CD106 ELISA Kit, human 100 

sVCAM-1 / CD106 ELISA Kit, mouse 98, 100 

sVCAM-1/CD106 ELISA Kit, human 97 

sVE-Cadherin / CD144 ELISA Kit, human 100, 102 

sVE-Cadherin / CD144 ELISA Kit, monkey 101, 102 


T, U 

TACI EDK, human 105 

TACI, human, recombinant 122 

TAFA-2, human, recombinant 122 

Tamoxifen citrate 46 

TARC (CCL17), human, recombinant 122 

TECK (CCL25), human, recombinant 122 

TECK ELISA Kit, mouse 98 

Tetrabromorhodamine 123, bromide 14 

Tetramethylrhodamine-alpha-Bungarotoxin 17 

Texas Red-DHPE 15 

TexasRed PCR Labeling Kit 53 

TexasRed-dUTP (PCR) 55 

TFF-1, human, recombinant 122 



TGF-alpha ELISA Kit, human 96 

TGF-alpha, human, recombinant 122 

TGF-beta 1 ELISA Kit, human 96 

TGF-beta 1 ELISA Kit, mouse 98 

TGF-beta 1 ELISA Kit, rat 99 

TGF-beta 1, human, recombinant (CHO) 122 

TGF-beta 1, human, recombinant (HEK) 122 

TGF-beta 2 ELISA Kit, human 96 

TGF-beta 2, human, recombinant (HEK) 122 

TGF-beta 2, human, recombinant (Sf9) 122 

TGF-beta 3, human, recombinant (CHO) 122 

TGF-beta 3, human, recombinant (E. coli) 122 

TGF-betaR2, human, recombinant 122 

Thapsigargin 46 

Thrombomodulin (CD141) 122, 135 

Thymosin alpha-1 ELISA Kit, human 101 

Thymosin beta-4 ELISA Kit, human 101 

Thymulin ELISA Kit, human 101 

Thyreoglobulin (hTG) ELISA Kit, human 103 

Thyreoglobulin (hTG, recovery) ELISA Kit, human 103 

TIE-2 ELISA Kit, human 96

158 



TIMP-1 ELISA Kit, human 96 

TIMP-1 ELISA Kit, mouse 98 

TIMP-1 ELISA Kit, rat 99 

TIMP-1, human, recombinant 122 

TIMP-2 ELISA Kit, human 96 

TIMP-2 ELISA Kit, mouse 98 

TIMP-2, human, recombinant 123 

TL-1A EDK, human 105 

TL-1A, human, recombinant 123 

TLR-3 (CD283), human, recombinant 123, 135 

TMRE 14, 17 

TMRM 14, 17 

TNF-alpha (total) ELISA Kit, human 96 

TNF-alpha (total) ELISA Kit, monkey 99 

TNF-alpha EDK, human 105 

TNF-alpha EDK, mouse 106 

TNF-alpha EDK, rat 106 

TNF-alpha ELISA Kit, human 96 

TNF-alpha ELISA Kit, mouse 98 

TNF-alpha ELISA Kit, rat 99 

TNF-alpha M, human, recombinant 123 

TNF-alpha, human, recombinant (E. coli) 123 

TNF-alpha, human, recombinant (Plant) 123, 132 

TNF-alpha, human, recombinant (Yeast) 123 

TNF-alpha, mouse, recombinant (E. coli) 129 

TNF-alpha, mouse, recombinant (S. lividans) 129 

TNF-alpha, rat, recombinant 130 

TNF-beta ELISA Kit, human 96 

TNF-beta, human, recombinant (E. coli) 123 

TNF-beta, human, recombinant (InC) 123 

TNF-beta, human, recombinant (Plant) 123, 132 

TNF-R (60 kDa) ELISA Kit, human 97 

TNF-R (80 kDa) ELISA Kit, human 97 

TNF-R I ELISA Kit, monkey 99 

TNF-R II ELISA Kit, human 97 

TNF-Receptor Fusion Protein, human, recombinant 123 

TNFRSF17 (CD269), human, recombinant 123, 135 

t-PA ELISA Kit, human 97 

TPEN 20 

TPO (MDGF) EDK, human 105 

TPO ELISA Kit, human 97 

TPO ELISA Kit, mouse 98 

TPO, human, recombinant (CHO) 123 

TPO, human, recombinant (E. coli) 123 

TPO, mouse, recombinant 129 

TPO, rat, recombinant 130 

TRAIL (114-281 aa), human, recombinant 123 

TRAIL (Apo2L), human, recombinant 123 

TRAIL (Apo2L), mouse, recombinant 129 

TRAIL ELISA Kit, human 97, 102 

TRITC-DHPE 15 

Trypan Blue Solution (0.4%) 14 

TSG , human, recombinant 123 

TSLP EDK, human 105 

TSLP, human, recombinant 123 

TWEAK EDK, human 105 

TWEAK Receptor, human, recombinant 123 

TWEAK, human, recombinant 123 

Tyrphostin AG 1295 46 

Tyrphostin AG 490 46 

Urotensin II ELISA Kit, human 103 

V 

VAP-1 ELISA Kit, human 97 

Vaspin, human, recombinant 124 

VCAM-1 (CD106), human, recombinant 124, 134 

VDBP ELISA Kit, human 103 

VEGF EDK, human 105 

VEGF EDK, mouse 106 

VEGF, rat, recombinant 130 

VEGF-121, human, recombinant (E. coli) 124 

VEGF-121, human, recombinant (Plant) 124, 133 

VEGF-121, human, recombinant (Sf9) 124 

VEGF-121, mouse, recombinant 129 

VEGF-145, human, recombinant (Sf9) 124 

VEGF-164, mouse, recombinant (InC) 129 

VEGF-164, mouse, recombinant (Plant) 129, 133 

VEGF-165, human, recombinant (CHO) 124 

VEGF-165, human, recombinant (E. coli) 124 

VEGF-165, human, recombinant (HEK) 124 

VEGF-165, human, recombinant (Plant) 124, 133 

VEGF-165, human, recombinant (Yeast) 124 

VEGF-165, mouse, recombinant (E. coli) 129 

VEGF-165, mouse, recombinant (Sf9) 129 

VEGF-189, human, recombinant 124 

VEGF-A ELISA Kit, human 97 

VEGF-A ELISA Kit, mouse 98 

VEGF-A ELISA Kit, rat 99 

VEGF-B, human, recombinant 124 

VEGF-C ELISA Kit, human, human 97 

VEGF-C ELISA Kit, rat 99 

VEGF-C, human, recombinant 124 

VEGF-C, rat, recombinant 130 

VEGF-C152S, rat, recombinant 130 

VEGF-D, human, recombinant 124 

VEGF-E, Orf virus, recombinant 124 

VEGF-I (TNFSF15), human, recombinant 124 

VEGF-R I ELISA Kit, human 97 

VEGF-R II ELISA Kit, human 97 

Visfatin, human, recombinant 125 

Visfatin, mouse, recombinant 129 

Vitamin D (direct) ELISA Kit, human 103 

Vitronectin, human, recombinant 125



W 

WIF-1, human, recombinant 125 

WISP-1, human, recombinant 125 

WISP-3, human, recombinant 125 

WNT-1, human, recombinant 125 

WNT-3a, mouse, recombinant 129 

X, Y, Z 

X-Gal 12 

Zinquin, ethyl ester 20 

Zinquin, free acid 21 

Zonulin ELISA Kit, human 103

160 Alphabetical International Index 

Distributors 

International Distributors 

Albania 

Biomedica Shpk 

Rr Themistokli Germenji 

Kulla Ambasador, Kati 3, Ap 1 

1000 Tirana 

Albania 

Phone: +355-69 40 91 613 

Fax: +355-45 00 681 

Email: b.shehu@bmgrp.al 

Internet: www.biomedica.co.at 

Australia 

Banksia Scientific Company 

3/160 Lytton Road 

PO Box 529 

Bulimba QLD 4171 

Australia 

Phone: +61-7-39 02 30 00 

Fax: +61-7-32 17 98 69 

Free Call: 1300 769944 

Email: sales@banksiascientific.com.au 

Internet: www.banksiascientific.com.au 

Austria 

Biomedica Medizinprodukte GmbH & Co KG 

Divischgasse 4 

1210 Wien 

Austria 

Phone: +43-1-29 10 75 6 

Fax: +43-1-29 01 42 9 

Email: sales.biomedica@bmgrp.at 


Bangladesh 

Krishgen BioSystems 

135/37 Zaveri Bazar 

Mumbai 400002 

India 

Phone: +91-22-66 37 29 90 

Fax: +91-22-66 37 29 91 

Email: sales@krishgen.com 

Internet: www.krishgen.com 

Bosnia-Herzegovina 

Biomedica d.o.o 

Zmaja od Bosne 4 

71000 Sarajevo 

Bosnia 

Phone: +387-66 90 60 86 

Email: a.hodzic@bmgrp.ba 


Bulgaria 

Sofbiolife-Biomedica Ltd. 

52, Gen. Gurko Str. Floor 5 

1000 Sofia 

Bulgaria 

Phone: +359-2-98 89 151 

Fax: +359-2-98 11 883 

Email: office@biomedica-bg.eu 

Internet: www.biomedica-bg.eu 

China 

Shanghai Weike Biochemical 

Reagent Co. Ltd. 

Room 3205, Universial Mansion 

No. 172 Yu Yuan Road 

200040 Shanghai 

Phone: +86-21-62 49 37 54 

Fax: +86-21-62 49 03 34 

Email: info@weike21.com 

Internet: www.weike21.com 

Croatia 

Biomedica Dijagnostika d.o.o. 

V Ravnice 10 

10000 Zagreb 

Croatia 

Phone: +385-91-22 66 39 4 

Email: a.dedic@bmgrp.at 


Czech Republic 

Biomedica CS, s.r.o. 

Na Šafránce 1651/9 

101 00 Praha 10 

Czech Republic 

Phone: +420-2-83 93 14 85 

Fax: +420-2-83 93 25 07 

Email: info@biomedica.cz 

Internet: www.biomedica.cz 

Denmark 

Labinova AB 

Centralvägen 2-4 

194 82 Upplands Väsby 

Sweden 

Phone: +46-8-59 03 24 90 

Fax: +46-8-43 74 32 02 

Email: info@labinova.se 

Internet: www.labinova.se 

Finland 

Biotop Oy 

Tykistökatu 6B 3 

20520 Turku 

Finland 

Phone: +358-2-241 00 99 

Fax: +358-2-241 01 11 

Email: myynti@biotop.fi 

Internet: www.biotop.fi 

Hungary 

Biomedica Hungaria Kft. 

Ribary u. 11 

1022 Budapest 

Hungary 

Phone: +361-22 53 85 0 

Fax: +361-20 12 68 4 

Email: biomed@biomedica.hu 

Internet: www.biomedica.hu 

India 



Mumbai 400002 

India 

Phone: +91-22-66 37 29 90 

Fax: +91-22-66 37 29 91 



Indonesia 



Mumbai 400002 

India 

Phone: +91-22-66 37 29 90 

Fax: +91-22-66 37 29 91 


Internet: www.krishgen.com

International Alphabetical Distributors Index 161 

Israel 

Biological Industries Ltd. 

Kibbutz Beit Haemek, 25115 

Israel 

Phone: +972-4-996-0595 

Fax: +972-4-996-8896 

Email: info@bioind.com 

Internet: www.bioind.com 

Italy 

International PBI S.p.A. 

Via Novara, 89 

20153 Milano 

Italy 

Phone: +39-2-48 77 91 

Fax: +39-2-40 09 00 10 

Email: info@internationalpbi.it 

Internet: www.internationalpbi.it 

Japan 

Funakoshi Co. Ltd. 

9-7 Hongo 2-chome 

Bunkyo-ku 

Tokyo 

Japan 113-0033 

Phone: +81-3-56 84 16 20 

Fax: +81-3-56 84 17 75 

Email: reagent@funakoshi.co.jp 

Internet: www.funakoshi.co.jp 

Korea 

Sungwoo Life Science Co., Ltd 

Samsung BLDG 4th Floor 560-5 

Uijeongbu 2Dong Uijeongbu-City 

Kyeonggi-Do 

South Korea 

Phone: +82-2-985-7471 

Fax: +82-31-876-9149 

Email: sungwoo@sungwools.com 

Internet: www.sungwools.com 

Macedonia 

Biomedica Dijagnostika dooel 

Ul Key 13-ti Noemvri br. 1 (GTC) 

1000 Skopje 

Macedonia 

Phone: +43-67 68 31 07 727 

Fax: +43-12 91 07 63 727 

Email: a.mitov@bmgrp.al 


Malaysia 

Biomarketing Services SDN BHD 

21, Jalan 4/62A 

Bandar Menjalara, Kepong 

52200 Kuala Lumpur 

Malaysia 

Phone: +603 6273 3068 

Fax: +603 6272 0093 

Email: info@biomarketing.com.my 

Internet: www.biomarketing.com.my 

Nepal 



Mumbai 400002 

India 

Phone: +91-22-66 37 29 90 

Fax: +91-22-66 37 29 91 



New Zealand 

Banksia Scientific Company 

12 Brigade Street 

Airport Oaks 

Auckland 

New Zealand 

Phone: +64-9-921 26 26 

Fax: +64-9-921 26 27 

Email: sales@banksiascientific.co.nz 

Internet: www.banksiascientific.co.nz 

Norway 

Labinova AB 

Centralvägen 2-4 

194 82 Upplands Väsby 

Sweden 

Phone: +46-8-59 03 24 90 

Fax: +46-8-43 74 32 02 

Email: info@labinova.se 

Internet: www.labinova.se 

Poland 

Biomedica Poland sp. z.o.o. 

Ul. Raszyńska 13 

05-500 Piaseczno 

Poland 

Phone: +48-22 73 75 996 

Fax: +48-22 73 75 994 

Email: biomedica@bmgrp.pl 

Internet: www.biomedica.pl 

Portugal 

Labclinics, S.A. 

c/Industria 54 

08025 Barcelona 

Spain 

Phone: +34-93-446 47 00 

Fax: +34-93-348 10 39 

Email: info@labclinics.com 

Internet: www.labclinics.com 

Romania 

CYBER SRL Romania 

Str. Smaranda Braescu, nr. 20D 

Sector 1, Cod 014203 

70941 Bucharest 

Romania 

Phone: +40-37 27 66 844 

Fax: +40-21 23 22 204 

Email: dan.pavel@cybermed.ro 

Internet: www.cyber-srl.ro 

Serbia 

Biomedica MP d.o.o. 

Djordja Pantelica 31 

11080 Zemun 

Serbia 

Phone: +381-11- 21 02 264 

Fax: +381-11- 21 02 264 

Email: bjelenic@biomedicamp.com 


Singapore 

Biomed Diagnostics Pte. Ltd. 

18 Boon Lay Way 

#05-105-108 TradeHub 21 

Singapore 609966 

Phone: +65-6-298 43 47 

Fax: +65-6-298 47 23 

Email: sales@biomed.com.sg 

Internet: www.biomed.com.sg

162 International Alphabetical Index Distributors 

Slovakia 

Biomedica Slovakia s.r.o. 

Drobného 27 

84102 Bratislava 

Slovakia 

Phone: +421-2-69 30 99 01 

Fax: +421-2-69 30 99 08 

Email: biomedica@biomedica.sk 

Internet: www.biomedica.sk 

Slovenia 

Biomedis M.B. 

Slokanova ulica 12 

2000 Maribor 

Slovenia 

Phone: +386-2-47 16 300 

Fax: +386-2-47 16 304 

Email: info@biomedis-mb.si 

Internet: www.biomedis-mb.si 

South Korea 

Sungwoo Life Science Co., Ltd 

Samsung BLDG 4th Floor 560-5 

Uijeongbu 2Dong Uijeongbu-City 

Kyeonggi_Do 

South Korea 

Phone: +82-2-985-7471 

Fax: +82-31-876-9149 

Email: sungwoo@sungwools.com 

Internet: www.sungwools.com 

Spain 

Labclinics, S.A. 

c/Industria 54 

08025 Barcelona 

Spain 

Phone: +34-93-446 47 00 

Fax: +34-93-348 10 39 

Email: info@labclinics.com 

Internet: www.labclinics.com 

Sri Lanka 



Mumbai 400002 

India 

Phone: +91-22-66 37 29 90 

Fax: +91-22-66 37 29 91 



Sweden 

Mediqip AB 

Förrådsvägen 10 

141 46 Huddinge 

Sweden 

Phone: +46-8-448 10 10 

Fax: +46-8-448 10 75 

Email: info@mediqip.se 

Internet: www.mediqip.se 

Taiwan 

Biochiefdom International Co. Ltd. 

1F, No 50, Yishou St. 

Wensham District 

Taipei 116 

Taiwan 

Phone: +886-2-29 37 00 88 

Fax: +886-2-29 37 97 17 

Email: info@bio-chief.com.tw 

Internet: www.bio-chief.com.tw 

Thailand 

Biomed Diagnostics (Thailand) Co. Ltd. 

96/38 Moo 6 Bangkruai-Sainoi Rd 

Bangkruai 

Nontaburi, 11130 

Thailand 

Phone: +66-2-879 60 26 

Fax: +66-2-879 60 65 

Email: nan@biomedthai.com 

Internet: www.biomedthai.com

164 

Notes 

Notes

Notes 

Notes 165

166 

Terms and Conditions 

Terms and Conditions 

General Terms 

1. These conditions of sale and delivery 

apply to all contracts of supply between 

us and our customers, even when in individual 

cases they are not referred to. 

2. A customer’s inconsistent conditions 

of purchase shall have no validity, even 

when we do not expressly reject such 

conditions. 

3. The customer is deemed to agree to 

these conditions of sale and delivery 

upon unconditional acceptance of the 

goods supplied. 

4. Rights of the customer arising during 

the course of business with us are not 

transferable. 

5. Any agreement that differs from these 

conditions of sale and delivery, or any 

recognition of the customer’s contradictory 

purchase conditions, requires our 

express confirmation in writing. 

Offers 

1. In the absence of a declaration to the 

contrary, we are not bound by any offers 

we make. 

2. Supply contracts only take effect when 

confirmed by us in writing. 

3. Any inquiries or complaints concerning 

confirmation are to be made promptly in 

writing. 

4. Assurances and assured properties 

only exist when they are expressly described 

as such. The descriptions and 

other statements in our catalogues and 

price lists are accurate to the best of our 

knowledge and belief. Under no circumstances 

may any assurances as to properties 

be derived from such descriptions 

and statements. 

Prices 

1. Our prices quoted in the current price 

list are net prices, VAT (value added tax) 

not included. 

2. VAT is charged according to the applicable 

laws in effect. 

3. Charges for delivery, cooling and insurance 

are indicated in the valid price 

list. 

4. PromoCell prices are subject to change 

without prior notice. 

Shipping charges 

Europe: 19 € 

United Kingdom: 13 £ 

USA / Canada: 45 $ 

For other countries 

please inquire! 

Delivery 

1. Delivery times will be specified after 

careful co-ordination. They are however 

not legally binding. Claims for compensation 

due to delayed or non-delivery are 

excluded. 

2. If a delivery date specified by us is 

exceeded by more than two weeks, the 

customer is entitled to give us a further 

two week time limit for delivery. Should 

the delivery not be carried out by the 

end of the additional two week delivery 

period, the customer is entitled to rescind 

the contract. The rescission must 

be made promptly and in writing, at the 

latest within a week of the expiry of the 

additional two week delivery period. 

3. All deliveries take place at the customer’s 

risk. The passing of risk takes place 

on the handing over of the goods to the 

carrier or haulier. The careful choice of 

dispatch method and means of transport 

is at our discretion with exclusion of any 

and all liability. 

4. Deliveries in part are permitted. They 

are to be regarded as separate deliveries. 

Payment 

1. Payment is to be made without any 

deductions within 14 days of delivery 

and remittance of the bill. 

2. Default of payment will result in legal 

proceedings upon the expiry of the payment 

period without further warning. In 

this case, or in the case of a warning, we 

are entitled to interest for late payment 

at the current normal bank borrowing 

rate, or alternatively at a rate of 5% 

above the current discount rate of the 

Deutsche Bundesbank. 

3. A cash discount is only permitted 

when expressly stated on the bill and 

when payment is made within the stated 

period. 

4. An offset against or demand for 

payment is only permitted when the 

customer has an undisputed or legally 

established counterclaim. 

5. If our conditions of payment are not 

complied with or if justifiable doubts 

exist regarding the customer’s ability or 

willingness to pay, we are entitled to 

rescind the contract and to demand the 

return of the delivered goods, the costs 

of delivery to be borne by the customer. 

In addition, all time outstanding claims 

will become due. 

Justifiable doubts referred to above 

include, but are not restricted to: unfavourable 

information, deterioration of 

financial circumstances, initiation of insolvency 

or bankruptcy proceedings, execution 

measures against the customer, 

protest of bills, and default of payment 

for any other reason. 

6. If the customer does not take delivery 

of properly delivered goods, we are 

entitled, after setting an additional time 

limit, to rescind the contract, or to damages 

for non-compliance with the contract. 

In the latter case we are entitled to 

demand either damages of 25% of the 

sales price without proof, or the actual 

amount of damages. 

Complaint 

1. Any complaint regarding the goods, 

such as defects, lack of assured properties, 

wrong delivery and over- or underdelivery 

are, after immediate discontinuation 

of use, to be reported in writing. 

2. In the case of wrong delivery, the 

goods will be taken back at our expense 

and replaced with the goods ordered. 

Excess deliveries may similarly be sent 

back to us at our expense. In the case of 

under delivery, the customer may choose 

between a reduction in payment or complete 

fulfilment of the order. 

3. In the case of delivery of faulty goods 

we are entitled to replace the faulty 

goods with goods free of faults. Should 

the replacement delivery also be faulty, 

the customer may choose between a 

reduction in payment or cancellation of 

the contract. Claims for compensation 

are excluded except in the case of lack of 

assured properties.

Terms and Conditions 167 

4. The right to complain expires in the 

case of obvious or apparent defects two 

weeks after receipt of the goods, and 

in the case of non-apparent defects six 

months after receipt of the goods. 

5. Goods may only be returned by arrangement 

with us and with the issue 

of a return number. The goods must be 

properly packed. If returned goods are 

damaged due to the fault of the customer, 

the customer is obliged to pay 

compensation for such damage. 

6. In the case of delivery to traders the 

customer is to inspect the goods without 

delay upon receipt and to report 

any complaints to us immediately. If the 

customer fails to make such a report 

the goods will be deemed approved. In 

the case of a fault that is not apparent 

upon inspection, the complaint must be 

reported immediately upon discovery of 

the fault, otherwise the goods will be 

deemed approved as regards such fault. 

Use of Goods 

1. Goods delivered by us are intended 

exclusively for the purposes of research 

and may not be used on human beings, 

animals, in the household, or for any 

private use. We emphasise that the use 

of our products for diagnostic or therapeutic 

purposes is subject to current legal 

regulations. 

2. We do not accept any liability for the 

improper use of our products. We deliver 

only to commercial enterprises, resalers 

and public researcher, development and 

teaching establishments. Legal regulations 

require us to carefully check orders 

and to refuse delivery when there are 

signs of improper use of our products. 

Orders from students or other private 

individuals must be given from an official 

office of their institute or firm. 

3. We expressly oppose research or any 

uses which run counter to ethical, legal 

or political standards or principles within 

Germany and/or the import country, or 

whose ethical justification is the subject 

of public debate and dispute in the respective 

country (i. e. cloning experiments). 

We do not accept any liability for such 

research or use by our customers or 

by third parties who have received our 

products from our customers. The users 

commit themselves not to carry out any 

genome analysis or any genetic change 

on the cell cultures. 

Liability 

1. All products delivered by us are only 

to be handled by trained personnel who 

are well aware of any potential dangers. 

Should any liability for compensation 

arise as a result of damage caused by 

breach of this condition, the customer 

shall be liable to pay any such compensation. 

In all cases, current safety regulations 

are to be observed, especially in the 

case of cells of human or animal origin, 

which always represent a potential risk 

of infection. The lack of a danger symbol 

does not mean that the substances concerned 

are harmless. 

2. We accept no responsibility for damage, 

loss, or injury caused by incorrect 

handling or use in the household, on 

humans, or on animals. In as far as we 

have made confirmation of delivery dependent 

upon a specified use of certain 

products, the customer accepts full liability 

for any damage suffered by us as 

a result of the customer’s incorrect use 

of the product. In the case of products 

which may only be used in accordance 

with legal or official regulations, the customer’s 

order simultaneously counts as 

a declaration that the products will be 

used for a specific purpose in the above 

sense. 

3. Our customers are required without 

exception to apply the laboratory guidelines 

of the professional association of 

the chemical industry when dealing with 

our products. 

4. Passing on of our products to private 

individuals is not permitted. If as a result 

of a breach of this condition for whatever 

reason we should receive a claim for 

compensation from a private individual, 

we shall have a right of redress against 

the customer. 

Indemnification 

1. In addition to clause VI above, claims 

by the customer are excluded, in so far 

as they are not based upon intention or 

gross negligence. 

2. In the case of delivery to a trader this 

exclusion also applies to any damage 

caused by the intention or gross negligence 

of our business associates, carriers 

or employees. We accept no liability 

for damage caused by the behaviour of 

companies or individuals which sell our 

products. 

3. The sale of our products under their 

trade names for further commercial use 

does not mean that these products and 

their trade names may be freely used 

in general circulation. Some products, 

their production and use or their trade 

names are patented or protected trade 

names. The further commercial use of 

such products and their trade names occurs 

as regards a third party under the 

exclusive responsibility and liability of 

the customer. 

Miscellaneous 

1. German law shall apply to all contracts 

entered into by us, including those for 

deliveries abroad. We expressly reject the 

application of the Vienna UN-agreement 

relating to international sales contracts 

of 11. 04. 80. 

2. In the case of contracts with traders 

who do not belong to the traders specified 

in §4 of the code of commercial law, 

the place for fulfilment of contracts and 

the court of jurisdiction shall be Heidelberg. 

3. If any of the above conditions is held 

to be invalid, the validity of any of the 

other conditions shall not be affected. 

4. Only the German version of this Terms 

of Delivery and Payment is valid. This 

translation shall only serve as assistance 

for our customers. You can get a German 

version on request. 

PromoCell GmbH 

Sickingenstr. 63/65 

69126 Heidelberg 

Germany 

Managing Director: Dirk Hüttner 

AG Mannheim HRB 335480

168 

Ordering and Technical Support 

Ordering and Technical Support 

Ordering Information 

To place an order please use any of the 

following convenient options: 

Phone Fax Online 

Our customer service representatives are 

ready to assist you with ordering Monday 

through Friday 

North America 

1 – 866 – 251 – 2860 (toll free) 

Deutschland 

0800 – 776 66 23 (gebührenfrei) 

France 

0800 90 93 32 (ligne verte) 

United Kingdom 

0800 – 96 03 33 (toll free) 

Other Countries 

+49 6221 – 649 34 0 

Please copy the fax order form on the next 

page and send it to the following fax numbers: 

North America 

1 – 866 – 827 – 9219 (toll free) 

Deutschland 

0800 – 100 83 06 (gebührenfrei) 

France 

0800 90 27 36 (ligne verte) 


0800 – 169 85 54 (toll free) 


+49 6221 – 649 34 40 

Online ordering is available 24 hours/ 

day, 7 days per week. Our online ordering 

system is secure, fast, and convenient. 

Check out www.promokine.info for more 

information. 

Email 

Please send an e-mail to 

order@promokine.info and include the 

following information: 

Catalog number, name, size, and quantity 

Shipping and billing address 

Contact person name and telephone 

number 

PromoKine directly supplies Belgium, 

Canada, France, Germany, Ireland, 

Luxembourg, United Kingdom, the 

Netherlands, the United States, and 

Switzerland. All deliveries are carried 

out using our recommended carrier, unless 

an alternative agreement has been 

arranged with the customer. Delivery 

time and shipment costs are extremely 

favorable. Our local sales representatives 

and our multilingual customer service are 

looking forward to serving you! 

For current information on PromoKine 

distributors see www.promokine.info or 

contact us via e-mail or phone. 

Technical Support 

Providing excellent technical support 

for our products is our top priority. Our 

technical service team relies on years of 

laboratory experience to assist you with 

product selection and advice to maximize 

product performance. 

You may reach our technical support via 

telephone/fax (numbers see on the reverse 

side of the catalog) or sending an 

e-mail to: support@promokine.info 

Product Use 

Products are sold for research use only 

and are not intended for use in diagnostic 

procedures or for therapeutic and 

medical purposes. 

Trademarks Used in this Catalog 

ABI Prism ® (PE Corporation, Applied 

Biosystems Division) 

Alexa Fluor ® (Molecular Probes) 

Cy TM (Amersham Bioscience) 

Mastercycler ® (Eppendorf) 

FACS ® (Becton Dickinson and Co.) 

FuGENE ® (Roche Molecular Systems) 

LightCycler ® (Roche Molecular Systems) 

Lipofectamine TM (Invitrogen) 

MagTag TM (IBA) 

MitoCapture TM (BioVision) 

PromoCell ® (PromoCell) 

Rhodamine Green ® (Molecular Probes) 

Texas Red ® (Molecular Probes) 

One or more third-party patents may 

cover the PCR process.

Fax Order Form 

Shipping Address 

Invoice Address (if different from shipping address) 

Mr. Ms. Dr. Prof. 

Mr. 

Ms. Dr. Prof. 

First Name 

First Name 

Last Name 

Last Name 

Company / University 

Company / University 

Department / Institute 

Department / Institute 

Street, No 

Street, No 

ZIP, City 

ZIP, City 

Country 

Country 

Phone 

E-mail 

Order Details 

Catalog Number Product Name Quantity 

Please write in capital letters 

Please send me a copy of the 

Please send me your newsletters 

PromoCell catalog 

PromoCell 

PromoKine catalog 

PromoKine 

Date, Signature

PromoCell GmbH 

Sickingenstr. 63/65 

69126 Heidelberg 

Germany 

North America 

Phone: 1 – 866 – 251 – 2860 (toll free) 

Fax: 1 – 866 – 827 – 9219 (toll free) 

Deutschland 

Telefon: 0800 – 776 66 23 (gebührenfrei) 

Fax: 0800 – 100 83 06 (gebührenfrei) 

France 

Téléphone: 0800 90 93 32 (ligne verte) 

Téléfax: 0800 90 27 36 (ligne verte) 


Phone: 0800 – 96 03 33 (toll free) 

Fax: 0800 – 169 85 54 (toll free) 


Phone: +49 6221 – 649 34 0 

Fax: +49 6221 – 649 34 40 

Email: 

info@promokine.info 

Promoting sustainable 

forest management 

www.pefc.org 

www.promokine.info

Cell Biology Products

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