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42 2 Apoptosis 2.7 Cathepsin/Calpain Detection Kits Applications Cathepsin Activity Assay Kits Calpain Activity Assay Kits Screening for apoptotic cells The Cathepsin Activity Assay Kits are The activation of calpain is involved in Cathepsin and calpain detection fluorescence-based and utilize the pre- many physiological and pathological Features ferred substrate sequence for each cathepsin, labeled with AFC (amino-4-triflu- processes (e.g., apoptosis). The Calpain Activity Assay Kit provides a simple and oromethyl coumarin). Cell lysates and convenient means for analysing calpain Non-radioactive method other samples that contain cathepsins activity in apoptotic cells and other sam- Easy and fast procedure cleave the synthetic substrate to release ples. The assay is based on fluorometric Appropriate for various multiwell free AFC that can be easily quantified us- detection and quantitation of the cleav- formats and automation ing a fluorometer or a fluorescence plate age of a calpain substrate at 505 nm Compatible with common reader. The cathepsin assays are easy to using a fluorometer or a fluorescence 2 Apoptosis fluorometers/fluorescence plate readers perform and can be adapted to 96-well plate assays. Assay conditons have been optimized to obtain the maximal activ- plate reader. The kit contains positive and negative controls. Comparison of the fluorescence intensity from a treated Apoptosis can be mediated by mecha- ity. Active cathepsin as well as cathepsin sample with a normal control allows nisms other than the well-studied cas- inhibitors, and cathepsin antibodies (see determination of the changes in calpain pase-mediated cleavage cascade. There chapter 6) are also available separately. activity. Active calpain as well as calpain is growing knowledge that alternative inhibitors and calpain antibodies (see proteolytic enzymes such as the lyso- Please visit www.promokine.info, or see chapter 6) are also available separately. somal cathepsin proteases may initiate the PromoKine Price List to find more or propagate proapoptotic signals, but cathepsin products. Please visit www.promokine.info, or see it is currently unclear how cathepsin the PromoKine Price List to find more mediates apoptosis. calpain products. Product Size Catalog Number Cathepsin B Activity Assay Kit 100 assays PK-CA577-K140 Cathepsin D Activity Assay Kit 100 assays PK-CA577-K143 Cathepsin H Activity Assay Kit 100 assays PK-CA577-K145 Cathepsin K Activity Assay Kit 100 assays PK-CA577-K141 Cathepsin L Activity Assay Kit 100 assays PK-CA577-K142 Cathepsin S Activity Assay Kit 100 assays PK-CA577-K144 Calpain Activity Assay Kit 100 assays PK-CA577-K240 Cathepsin B, human (native) 5 µg PK-RP577-1021 Cathepsin D, human (native) 5 µg PK-RP577-1022 Cathepsin H, human (native) 5 µg PK-RP577-1023 Cathepsin L, human, recombinant 5 µg 100 µg PK-RP577-1135-5 PK-RP577-1135-100 Cathepsin S, human (native) 5 µg PK-RP577-1025 Calpain I, human (native) 100 µg PK-RP577-1134 Cathepsin B & L Substrate Z-FR-R110 5 mg PK-CA707-10209 Cathepsin Substrate Z-FR-AMC 25 mg PK-CA707-10201 Calpain Inhibitor Z-LLY-FMK 20 µl (10 mM) PK-CA577-1125-20C Cathepsin B&L Inhibitor Z-Phe-Phe-FMK 20 µl (10 mM) PK-CA577-1126-20C For prices please see our online shop at www.promokine.info
2 Apoptosis 43 2.8 Kinase Detection Kits Applications large numbers of samples. The assay Akt Activity Detection Kit utilizes an N-terminal c-Jun fusion pro- Screening for apoptotic cells tein bound to Glutathione-Sepharose The Akt Activity Detection Kit utilizes Kinase detection and activity beads to selectively “pull down” JNK an Akt-specific antibody to immunopre- screening from cell lysate. After a washing step to cipitate Akt from cell lysate. Akt-specific Features remove non-specifically bound proteins, the kinase reaction is carried out in the activity is then detected in a kinase reaction using recombinant GSK-3a as presence of cold ATP. c-Jun phosphoryla- a substrate. Phosphorylation levels of Non-radioactive method tion is then determined by Western blot GSK-3a are determined by Western blot Easy, accurate, and fast procedure analysis using a phospho-c-Jun specific analysis using a phospho-GSK-3a spe- Compatible with standard lab antibody. cific antibody included in the kit. The kit equipment The activation of protein kinases, such as JNK and Akt, plays an important role JNK Activity Detection Kit The JNK Activity Detection Kit utilizes specifically detects Akt1, Akt2, and Akt3 activities. Other kinase activities are not detected. Active JNK and Akt kinases as well 2 Apoptosis in apoptosis and other physiological and a JNK-specific antibody to immuno- as JNK/Akt substrates, activators, in- pathological processes. PromoKine pro- precipitate JNK from cell lysates. The hibitors, and control cell lysates are also vides a novel non-radioactive system for activity of the JNK is then detected in a available separately. convenient measurement of JNK and kinase reaction using recombinant c-Jun Please visit www.promokine.info or see Akt activities. as substrate. c-Jun phosphorylation is the PromoKine Price List to find more JNK Activity Screening Kit determined by Western blotting using a phospho-c-Jun specific antibody. The active kinases that can be used for cell analysis and apoptosis research. A kit specifically detects JNK activity; other range of kinase antibodies can be found The JNK Activity Screening Kit can kinase activities are not detected. in chapter 6. screen JNK activity quickly even with Product Size Catalog Number JNK Activity Screening Kit 40 assays PK-CA577-K430 JNK Activity Detection Kit 40 assays PK-CA577-K431 Akt Activity Detection Kit 40 assays PK-CA577-K435 Akt Inhibitor 1 mg PK-CA577-1701 Chk2 Inhibitor 1 mg PK-CA577-1702 c-Jun (1-79)-GST Fusion Protein 100 ug PK-CA577-7001 GSK-3a-GST Fusion Protein 100 ug PK-CA577-7003 GSK-3β, human, recombinant 100 ug PK-CA577-7004 L-JNK Peptide Inhibitor I 50 µl (1 mM) PK-CA577-7011 L-JNKI1 Negative Control Peptide 50 µl (1 mM) PK-CA577-7021 JNK Negative Control Cell Lysate 1 mg PK-CA577-7031 JNK Activated Cell Lysate 1 mg PK-CA577-7032 Akt Negative Control Cell Lysate 1 mg PK-CA577-7035 Akt Activated Cell Lysate 1 mg PK-CA577-7036 For prices please see our online shop at www.promokine.info For a list of active recombinant kinases, kinase substrates, inhibitors, and antibodies please visit our website www.promokine.info, or see the PromoKine Price List.
Page 1 and 2: Cell Biology Products 2011 | 2012
Page 3 and 4: Overview 1 Overview 1 Cell Analysis
Page 5: Contents 3 4 5 6 7 8 Gene Cloning,
Page 8 and 9: 6 Contents Chapter 1 1 Cell Analysi
Page 10 and 11: 8 1 Cell Analysis Bioluminescent Ce
Page 12 and 13: 10 1 Cell Analysis 1.3 Cytotoxicity
Page 14 and 15: 12 1 Cell Analysis Product Size Cat
Page 16 and 17: 14 1 Cell Analysis Product Product
Page 26 and 27: 24 1 Cell Analysis 1.9 Cell Stress
Page 28 and 29: 26 1 Cell Analysis 1.10 Cell Fracti
Page 30 and 31: 28 1 Cell Analysis 1.11 Nucleic Aci
Page 33 and 34: Apoptosis 2 Apoptosis, or programme
Page 35 and 36: 2 Apoptosis 33 2.1 DNA Fragmentatio
Page 37 and 38: 2 Apoptosis 35 2.4 Glutathione Dete
Page 39 and 40: 2 Apoptosis 37 Product Size Catalog
Page 41 and 42: 2 Apoptosis 39 Caspase Screening Ki
Page 43: 2 Apoptosis 41 Product Size Catalog
Page 47 and 48: 2 Apoptosis 45 2.10 Apoptotic Cell
Page 49 and 50: Fluorescent Labeling 3 The availabi
Page 51 and 52: 3 Fluorescent Labeling 49 3.1 Promo
Page 53 and 54: 3 Fluorescent Labeling 51 Excitatio
Page 55 and 56: 3 Fluorescent Labeling 53 3.2.2 Pro
Page 57 and 58: 3 Fluorescent Labeling 55 3.3.3 Pro
Page 59 and 60: 3 Fluorescent Labeling 57 3.4 Phyco
Page 61 and 62: 3 Fluorescent Labeling 59 3.5 Phyco
Page 63 and 64: Gene Cloning, Expression, 4 and Sil
Page 65 and 66: 4 Gene Cloning, Expression, and Sil
Page 73: 4 Gene Cloning, Expression, and Sil
Page 76 and 77: 74 Contents Chapter 5 5 Cell Transf
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Page 80 and 81: 78 5 Cell Transfection n Some cell
Page 82 and 83: 80 5 Cell Transfection Phase contra
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Page 87 and 88: Antibodies and ELISAs 6 Antibodies
Page 89 and 90: 6 Antibodies and ELISAs 87 6.1 Mono
Page 91 and 92: 6 Antibodies and ELISAs 89 n Kinase
Page 93 and 94: 6 Antibodies and ELISAs 91 6.2 Labe
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6 Antibodies and ELISAs 93 6.3 ELIS
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6 Antibodies and ELISAs 95 Product
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Cytokines and Growth 7 Factors Cyto
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7 Cytokines and Growth Factors 109
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138 Contents Chapter 8 8 Microbial
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140 8 Microbial Detection and Elimi
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142 8 Microbial Detection and Elimi
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144 Alphabetical Index Alphabetical
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146 Alphabetical Index Description
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160 Alphabetical International Inde
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162 International Alphabetical Inde
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Notes Notes 165
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Terms and Conditions 167 4. The rig
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Fax Order Form Shipping Address Inv
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