Cell Biology Products
Cell Biology Products
Cell Biology Products
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1 <strong>Cell</strong> Analysis 13<br />
1.6 <strong>Cell</strong> Staining Reagents and Related <strong>Products</strong><br />
Applications<br />
Detection of cellular structures<br />
Fluorescence microscopy/Flow<br />
cytometry<br />
Features<br />
High quality<br />
Thoroughly tested<br />
Highly sensitive<br />
Fluorescent dyes offer extraordinary sensitivity<br />
and extended possibilities for the<br />
visualization and analysis of intracellular<br />
processes and cell structures such as the<br />
cell membrane, mitochondria, the nucleus,<br />
and the cytoskeleton. Some dyes<br />
are also suited for staining only viable<br />
or dead cells. That is an important feature<br />
for elucidating the effects of certain<br />
agents on cell viability.<br />
PromoKine provides a select choice of<br />
reagents for staining different cell organelles<br />
and structures as well as for<br />
determining processes like membrane<br />
potential changes. Some of these reagents<br />
are available as esters, which pass<br />
through the membranes of viable cells.<br />
Once inside, the ester group is hydrolyzed<br />
by cellular esterases, and the dye<br />
can accumulate in the cell. Some dyes<br />
are nonfluorescent in their ester form but<br />
fluoresce strongly when the ester group<br />
has been removed.<br />
Fig. 7: Primary human fibroblasts (NHDF,<br />
Promo<strong>Cell</strong>) stained with Calcein and<br />
counterstained with DAPI.<br />
1 <strong>Cell</strong> Analysis<br />
Product Product Description Size Catalog Number<br />
Nucleus Stains<br />
7-AAD<br />
(7-aminoactinomycin D)<br />
Fluorescent DNA intercalator 1 mg PK-CA707-40037<br />
Acridine Orange (AO)<br />
Solution<br />
Nucleus stain (DNA/RNA)<br />
10 ml<br />
(10 mg/ml)<br />
PK-CA707-40039<br />
DAPI Nucleus stain (DNA) 10 mg PK-CA707-40011<br />
DAPI Solution Nucleus stain (DNA); aqueous solution 10 mg in 1 ml PK-CA707-40043<br />
DAPI, dilactate 4’,6-diamidino-2-phenylindole, dilactate 10 mg PK-CA707-40009<br />
EB (ethidium bromide)<br />
Solution<br />
Dead cell stain (DNA), aqueous solution<br />
10 ml<br />
(10 mg/ml)<br />
PK-CA707-40042<br />
Ethidium Homodimer I Nucleus stain (DNA); also known as EthD-1, or EtDi 1 mg PK-CA707-40010<br />
Ethidium Homodimer III Nucleus stain (DNA) 1 mg PK-CA707-40050<br />
Ethidium Homodimer III, Nucleus stain (DNA); in DMSO 200 µl PK-CA707-40051<br />
1 mM in DMSO<br />
Hoechst 33258 Nucleus stain (DNA) 100 mg PK-CA707-40045<br />
Hoechst 33258 Solution Nucleus stain (DNA); aqueous solution 10 ml PK-CA707-40044<br />
(10 mg/ml)<br />
Hoechst 33342 Nucleus stain (DNA) 100 mg PK-CA707-40047<br />
Hoechst 33342 Solution Nucleus stain (DNA); aqueous solution 10 ml PK-CA707-40046<br />
(10 mg/ml)<br />
Propidium Iodide (PI) Dead cell stain (DNA) 100 mg PK-CA707-40016<br />
Propidium Iodide (PI) Solution Dead cell stain (DNA); aqueous solution 10 ml PK-CA707-40017<br />
(1 mg/ml)<br />
Mitochondria Stains<br />
DASPEI<br />
2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide;<br />
100 mg PK-CA707-70018<br />
for staining of mitochondria in live cells<br />
Dihydrorhodamine 123 Reduced form of Rhodamine 123; mitochondria stain 10 mg PK-CA707-10055<br />
Dihydrorhodamine 123,<br />
dihydrochloride salt<br />
DiOC 6<br />
(3)<br />
JC-1 chloride<br />
Reduced form of Rhodamine 123; mitochondria stain 10 mg PK-CA707-10056<br />
3,3’-dihexyloxacarbocyanine, iodide; membrane<br />
potential sensitive dye<br />
Stains mitochondria in living cells in a membrane<br />
potential-dependent function<br />
For prices please see our online shop at www.promokine.info<br />
100 mg PK-CA707-70009<br />
5 mg PK-CA707-70011
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