THE ALEXANDER FLEMING PAPERS - British Library

This notebook has been divided into named sections. However, as it is a
ring binder it is difficult to know if the papers are in the correct order or if
sheets have been added later on.
Notebook includes:
Section one: 8 Feb.-2 May 1924.
ff. 1-6. Children – Bactericidal and Phagocytosis
i) Experiment examining phagocytosis and children
ii) Experiment examining phagocytosis and killing in infant blood
iii) Child’s blood bactericidal in slide cells
iv) Diagram of normal blood and blood infected with mastron
septicaemia
Section two: 20 Jan. 1925-19 June 1926.
ff. 7-12. Granules Leucocytes
i) Notes on a septic uterus
ii) Notes on a subphrenic abscess
iii) Experiment with a rabbit which has been given Le Cinnate
intravenously and comparison made of samples of blood taken
before and after the injection
iv) Experiment to examine AF blood two days after the onset of a
cold
v) Notes on leucocytic dendrils. Took AF defibrinated blood, left
for half an hour and observations made on movement,
phagocytosis and staining, includes diagrams
Section three: 10 Sept. 1925-10 Jan. 1927.
ff. 13-35. Centrifuged Phagocytosis
i) Experiment examining ordinary opsonic versus centrifuged
pseudo phagocytosis
ii) Diagram of distribution of leucocytes corpuscles and bugs on
opsonic slide
iii) Experiment of Pseudo phagocytose – the affect of time of
centrifuging on phagocytosis, including diagrams
iv) Centrifuged opsonics
I – Rate of opsonisation
II – Time of centrifuging on phagocytosis
v) Centrifuged opsonics
I – Effect of different times of centrifuging on
opsonised and non opsonised bugs
II – Rate of opsonisation at 37˚C and 18˚C
vi) Experiment examining the affect of time of centrifuging with
fully opsonised staphylococcus
vii) Experiment testing staphylococcus opsonised half an hour
before phagocytosis
viii) Experiment examining the influence of temperature on
phagocytosis
ix) Time of incubating or centrifuging with fully opsonised bugs
(coli)
x) Temperature on phagocytosis