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THE ALEXANDER FLEMING PAPERS - British Library

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Section two: 13-23 Oct. 1924.<br />

ff. 3-5. Immunisation in vitro<br />

i) Experiments with fresh and incubated leucocytes<br />

ii) Experiment with de-leucocyted blood and leucocytes<br />

iii) Experiment with faeces filtrate<br />

Section Two: 12 May-15 Nov. 1926.<br />

ff. 6-9. [Bacteria/ bactericidal] slide<br />

i) Experiment with haemolytic B. coli in slide cells<br />

ii) Experiment with haemolytic coli in slide cells in salted blood<br />

iii) Experiment with different streptococcus in slide cells<br />

iv) Experiment to see if killing of staphylococcus various with<br />

thickness of the slide cell<br />

Section Three: n.d<br />

ff. 10-14. Worms (penicillin)<br />

i) Experiment with different bugs in slide cells<br />

ii) Diagram of the growth of staphylococcus in cells<br />

iii) Experiment examining the rate of emigration of leucocytes<br />

from worms<br />

Section four: 30 Oct. 1928.<br />

ff. 15-16. Discovery of Penicillin<br />

Description of a staphylococcus inhibiting mould, including diagrams<br />

Section five: 22 Nov. 1928-17 Jan. 1929.<br />

ff. 17-39. Experiments with the new ‘mould’<br />

i) Experiments with different bacteria including diagram of<br />

results<br />

ii) Inhibitory action of ‘mould’ broth of staphylococcus in blood<br />

iii) Inhibition by moulds, including diagram of plate<br />

iv) Mould inhibition and imbedded agar and mould filtrate<br />

streaked across bugs<br />

v) Mould planted in broth; 30 Nov.<br />

vi) Mould inhibitions – different penicillin from milk<br />

vii) Craddock – inhibitions streptococci by mould filtrate<br />

viii) Mould filtrate on Pfeiffer including diagram<br />

ix) Mould filtrate antiseptic power of Craddock’s autrium<br />

x) Conditions of optimum growth of inhibiting mould<br />

xi) Experiments autoclaving the mould filtrate<br />

Section six: 29 Jan.-9 March 1929.<br />

ff. 40-41, 43-48, 51, 55, 59. Inhibitory action<br />

i) Mould inhibitions – penicillin 12<br />

ii) Isolation Pfeiffer by mould inhibition<br />

iii) Formation of inhibitory substance in mould juice

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