THE ALEXANDER FLEMING PAPERS - British Library

papers are in the correct order. The notebook seems to have originally
housed plain paper and this has been supplemented with other pieces of
plain and lined paper.
Notebook includes:
Section one: 18 Sept. 1924-18 June 1928.
ff. 1-12. Lysozyme
i) On coli’s septicaemic streptococcus
ii) Experiment examining aqueous and vitreous humour
iii) Different animals on streptococcus jennies
iv) Experiment to see whether Jennis streptococcus is resistant to
lysozyme
v) Virulent and avirulent streptococcus – in agar cultures and the
effect of tears
vi) Versus antiseptics on lyso in agar plate
vii) On killing in slide cells, includes a note in red ink ‘therefore
lysozyme no effect on killing’
viii) Intracellular killing
ix) Rate of diffusion in agar
x) Experiments with plates imbedded with streaks of egg and
nasal mucus, includes coloured diagrams of results
Section two: 24 March 1925-14 Oct. 1926.
ff. 13-23. Intracellular Digestion
i) Autolysis and lysis with egg
ii) Different strains of staphylococci – experiment to see if they
are killed by blood equally
iii) Immediate killing – AF defibrinated blood and staphylococcus
iv) Inhibition of growth of staphylococcus in leucocytes (on bench
overnight). Includes diagrams with typical example of
leucocytes and typical bacteria outside leucocytes
v) Do washed leucocytes kill without serum and kill
staphylococcus in slide cells?
vi) Rate of killing of staphylococcus by leucocytes
Section three: 1 Nov.-13 Dec. 1927.
ff. 24-47. Bactericidal power of blood in virulent (non – phagocylable)
pneumococci
i) Does blood kill virulent pneumococci which are not
phagocyted?
ii) Typed notes – killing of pneumococci by normal blood and
blood to which immune serum has been added, includes
handwritten notes about slide cells and results after sample were
put in an incubator for 24 hours
iii) Experiment examining the affect of carbolic on staphylococcus
and pneumo in blood
iv) Pneumo killing by blood: