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here - the 34th European Brewery Convention

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Stir Bar Sorptive Extraction Liquid Desorption-GC-MS. Statistical analysis of 43 semi-quantified<br />

volatile compounds enabled <strong>the</strong> separation of <strong>the</strong> beers into distinct groups. White beers was <strong>the</strong> most<br />

complex group with high level of β-damascenone and linalol, geraniol, citronellol w<strong>here</strong>as ales<br />

contained high level of ethyl caproate, ethyl phenylacetate as well as 4-vinylguaicol.<br />

Lagers could be separated in 2 groups according to <strong>the</strong> amounts of hop volatile compounds (linalol,<br />

α/β eudesmol, methyl geranate).<br />

P29<br />

Addition of fermentable and non fermentable carbohydrates - impact on <strong>the</strong> yeast metabolism,<br />

sweetness, palate fullness and SO 2 -content in beer<br />

Torsten Seewald 1 , Christof Reinhardt 1 , Thomas Kunz 1 , Frank-Jürgen Methner 1<br />

1 Technische Universität Berlin, Lab of Brewing Science, Berlin, Germany<br />

Aim of this study was to investigate influences of fermentable and non-fermentable carbohydrate<br />

addition into brewing process prior fermentation to get a better insight in <strong>the</strong> influence on <strong>the</strong> yeast<br />

metabolism, sweetness, palate fullness and SO2-formation.<br />

Compared to <strong>the</strong> standard wort, <strong>the</strong> results demonstrate a general carbohydrate dependent increase<br />

in SO2-formation during fermentation. The highest increase in SO2 results from <strong>the</strong> fermentable<br />

sugars glucose and sucrose followed by <strong>the</strong> non-fermentable sugar isomaltulose and polydextrose. In<br />

correlation to <strong>the</strong> non-fermentable carbohydrate addition <strong>the</strong> additional SO 2 -formation mainly based on<br />

<strong>the</strong> increase in osmotic pressure is limited by <strong>the</strong> influences on <strong>the</strong> methionine pathway, <strong>the</strong><br />

acetaldehyde production and <strong>the</strong> activation of <strong>the</strong> sulphate-reductase.<br />

Dependent to <strong>the</strong> used sugar a sensory panel detected a reversal point w<strong>here</strong> <strong>the</strong> sweetness is<br />

coming to <strong>the</strong> fore and gets much stronger in comparison to <strong>the</strong> palate fullness.<br />

P30<br />

Investigation of <strong>the</strong> influence of a permease on diacetyl content during beer fermentation<br />

Nisha James 1 , Christine Lang 2 , Ulf Stahl 1<br />

1 Research and Teaching Institute for Brewing in Berlin (VLB), Department of Microbiology, Berlin,<br />

Germany, 2 TU Berlin, Berlin, Germany<br />

Yeasts secrete diacetyl during fermentation imparting an undesirable buttery flavour to beer. Gene<br />

targets for reducing diacetyl production were identified using transcriptome analysis. We identified<br />

GAP1 as a potential candidate in several genes. Gap1p functions as a transporter for amino acids and<br />

previous studies have shown that <strong>the</strong> availability of amino acids for <strong>the</strong> cell influences diacetyl<br />

production. We verified <strong>the</strong> transcriptome data by immunoblot analysis as Gap1p. We proposed <strong>the</strong><br />

hypo<strong>the</strong>sis that t<strong>here</strong> is a positive correlation between amounts of Gap1p and diacetyl. To verify this<br />

we analysed diacetyl production under varying Gap1p levels in WT, Δgap1 and Gap1p overexpression<br />

strains of S. cerevisiae. We also show a positive correlation between Gap1p expression level and<br />

diacetyl levels under brewing conditions using industrial yeast strains. Our results indicate that<br />

influencing Gap1p levels indeed leads to changes in diacetyl levels.<br />

P31<br />

A new insight for controlling <strong>the</strong> hop aroma using hydrophobicity of yeast cell surface

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