here - the 34th European Brewery Convention
here - the 34th European Brewery Convention
here - the 34th European Brewery Convention
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P83<br />
Making gluten-free beers with barley malt and a proline-specific endoprotease<br />
Sylvie M Van Zandycke 1 , Chantal Christis 2 , Luppo Edens 2 , Fredoen Valianpour 2 , Joze Mutsaers 2 , Ron<br />
Duszanskyj 2<br />
1 DSM Food Specialties, Enzyme Solutions, South Bend, United States, 2 DSM Food Specialties, Delft,<br />
Ne<strong>the</strong>rlands<br />
Gluten free beers are generally made with grains that do not contain gluten such as sorghum, rice,<br />
buckwheat… These beers are safe to drink for people who display sensitivities to gluten or have been<br />
diagnosed as having celiac disease; a potential life-threa<strong>the</strong>ning condition. It is also possible to<br />
produce beers that are low in gluten when grains containing gluten are used. Indeed, a large amount<br />
of gluten proteins are removed during <strong>the</strong> brewing process and additionally, a proline-specific<br />
endoprotease (PSEP) has been suggested as a mean to hydrolyze <strong>the</strong> remaining gluten proteins from<br />
barley malt to bring <strong>the</strong> levels down to undetectable (results vary according to dosage of <strong>the</strong> enzyme<br />
and method used to measure gluten). This paper will focus on <strong>the</strong> nature of PSEP and <strong>the</strong> process of<br />
making gluten-free beers with malting barley including <strong>the</strong> recommended procedures to measure<br />
gluten accurately and current levels of gluten in commercial beers.<br />
P84<br />
Potential of aroma-modified distiller's grains for <strong>the</strong> application in cereal based functional<br />
beverages<br />
Magdalena Rückert 1 , Martin Zarnkow 1 , Thomas Becker 1<br />
1 Technische Universität München, Center of Life Sciences Weihenstephan, Chair of Brewing and<br />
Beverage Technology, Freising, Germany<br />
Distiller's grains (DG), <strong>the</strong> cereal by-product coming up during <strong>the</strong> production process of fuel or<br />
beverage alcohol, are prevalently used as feed additives and material for biogas plants. DG contain all<br />
ingredients of <strong>the</strong> whole grain in a concentrated form, especially high amounts of protein and dietary<br />
fiber. The nutritional properties of DG provide a new interest in using DG as a food ingredient and as a<br />
substrate for cereal based functional beverages.<br />
One important reason for not using DG as a food ingredient yet is <strong>the</strong> characteristic flavor. Our aim is<br />
to analyze <strong>the</strong> aroma profile of DG from wheat and neutralize reasonable components for getting a<br />
modified proper flavor. T<strong>here</strong>fore <strong>the</strong> aroma of DG was defined trough sensorial analysis and aroma<br />
active components were analyzed with GC-MS. Extracts of DG <strong>the</strong>n were treated with charcoal and<br />
assessed by sensorial analysis.<br />
P85<br />
Simulation of mashing process<br />
Simon Weber 1 , Heiko Briesen 1<br />
1 Technische Universität München, Chair for Process Systems Engineering, Freising, Germany<br />
Starch hydrolysis by amylases is strongly influenced by <strong>the</strong> starch polymer structure (chain lengths<br />
and branches). Amylases binding <strong>the</strong> starch polymer have several subsites located around <strong>the</strong> active<br />
centre of <strong>the</strong> enzymes. T<strong>here</strong> exist several possibilities of polymer binding with different resulting<br />
binding energies (BE). With BE and <strong>the</strong> enzyme activities (EA) it is possible to calculate <strong>the</strong> resulting<br />
chain length distributions and hydrolysis rates. In this work we develop new models based on ordinary<br />
differential equations (ODE, fast but inflexible) and kinetic Monte-Carlo technique (kinMC, slow but