here - the 34th European Brewery Convention

P108
Transfer of Nitrate into Beer during Dry-hopping
Stefan Hanke 1 , Annika Lagemann 2 , Georg Stettner 3
1 Bitburger Braugruppe GmbH, Versuchsbrauerei, Bitburg, Germany, 2 Bitburger Braugruppe GmbH,
Central Laboratory, Bitburg, Germany, 3 Bitburger Braugruppe GmbH, Technology and Quality, Bitburg,
Germany
Within the last years the success of the US Craft beers, which are partial extremely hopped, also
inspired brewers outside the US. The technique of dry-hopping is a very old method to enhance flavor
properties of beer and was re-established by the craft movement. Since 2012, this hopping method is
also official allowed to be used in Germany and so the amount of dry-hopped beers in the market
increases steadily. During dry-hopping flavor and phenolic compounds migrate into the beer. The
migration of other compounds (like nitrate) was not investigated until today. The limit value for nitrate
in drinking water is set to 50 ppm. In our study beers were produced with different dry-hopping
regimes (up to 400 g/hl) and nitrate was measured in the final beers. It could be shown that significant
amounts of nitrate migrate into the beer and that the legal limit can be reached. This shows that nitrate
level in hops should be considered in case of dry-hopping.
P109
Beer and cardiovsculare system - immediate respons after intake
Ida Leskosek-Cukalovic 1 , Sasa Despotovic 1 , Mirjana Platisa 2 , Vera Gal 2 , Zorica Nestorovic 2 , Ljiljana
Gojkovic-Bukarica 3 , Mile Veljovic 1 , Sonja Pecic 1 , Viktor Nedovic 1
1 Institute for Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade,
Belgrade, Serbia, 2 Institute of Biophysics, Faculty of Medicine, University of Belgrade, Belgrade,
Serbia, 3 Institute of Clinical Pharmacology, Faculty of Medicine, University of Belgrade, Belgrade,
Serbia
Numerous long-term studies have described the impact of alcohol beverages on cardiovascular
diseases. In this study it was investigated the effect of acute intake of beer on blood pressure and
heart rate variability. The HRV analysis and ECG was recorded in supine position in 18 young healthy
subjects. Results of HRV analysis indicate on mild influence of beer on the autonomic cardiac control.
The influence of beer on heart rhythm, examined by the HRV analysis which quantify the activity of the
autonomic nervous system on the heart, was not significant when all the subjects were analyzed
together. However, the differences between subjects were significant for all variables. Therefore, it
was analyzed the effect of beer for each subject separately. In 10 out of 18 subjects, beer drinking
significantly changed HR and/or HRV measures. The effect of beer is the increase of HR and
corresponding decrease of autonomic modulation reflected on decrease of spectral powers and
parasympathetic activity.