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Viscin Cells in the Dwarf Mistletoe Arceuthobium ... - Davidsonia

Viscin Cells in the Dwarf Mistletoe Arceuthobium ... - Davidsonia

Viscin Cells in the Dwarf Mistletoe Arceuthobium ... - Davidsonia

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84<br />

Image: Cynthia M. Ross<br />

Figure 6. Whole visc<strong>in</strong> cells obta<strong>in</strong>ed from a seed collected 10 days follow<strong>in</strong>g explosive<br />

discharge and sta<strong>in</strong>ed with 2,3,5-triphenyl tetrazolium chloridescale (2,3,5-TTC).<br />

Note <strong>the</strong> obviously red-sta<strong>in</strong>ed nucleus, which is viable. n = nucleus; bar = 50 µm<br />

pole, <strong>the</strong> embryo will be drawn <strong>in</strong>to close contact with <strong>the</strong> host when<br />

<strong>the</strong> mucilage permanently dries. Secondly, <strong>the</strong> visc<strong>in</strong> cell helical walls<br />

recoil upon dry<strong>in</strong>g; when whole seeds were dried on glass plates, <strong>the</strong><br />

visc<strong>in</strong> cells shortened and <strong>the</strong> coil<strong>in</strong>g became visibly tighter, anchor<strong>in</strong>g<br />

<strong>the</strong> seed downward (Figure 5).<br />

<strong>Visc<strong>in</strong></strong> cell functions outside <strong>the</strong> fruit may depend on <strong>the</strong>ir biochemical<br />

activity. Scharpf and Parmeter (1962) exam<strong>in</strong>ed <strong>the</strong> viability of <strong>the</strong><br />

<strong>Arceuthobium</strong> embryos with <strong>the</strong> vital oxidation-reduction sta<strong>in</strong> 2,3,5-<br />

triphenyl tetrazolium chloride (2,3,5-TTC). We observed evidence of

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