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Opsporen in surveillance stalen - UZ Leuven

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Metallo-bèta lactamases<br />

Confirmatie <strong>in</strong> Enterobacteriaceae, P. aerug<strong>in</strong>osa, Ac<strong>in</strong>etobacter<br />

2. Inhibitor based confirmatie testen<br />

– MBL activiteit ~ Zn 2+<br />

Inhibitoren = Zn-chelatoren (e.g. EDTA, MPA, DPA)<br />

i. Cica-Beta test<br />

ii.<br />

– Te lage SN niet geschikt<br />

MBL Etest ® (IP/IPI)<br />

– Enkel bruikbaar <strong>in</strong>dien MIC > 4µg/ml<br />

– Te lage SN niet geschikt<br />

– Indien MIC ≤ 4 µg/ml niet <strong>in</strong>terpreteerbaar resultaat!<br />

– Meeste MBL-producerende Enterobacteriaceae: MIC < 4 µg/ml<br />

» Ondanks, aanbevolen door DWP voor MBL confirmatie <strong>in</strong><br />

Enterobacteriaceae

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