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Annual Report 2012 - African Agricultural Technology Foundation

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30 AATF <strong>Annual</strong> <strong>Report</strong> <strong>2012</strong><br />

Bacterial Wilt-Resistant Banana Project<br />

Banana cultivar lines<br />

with resistance to<br />

bacterial wilt disease<br />

developed in Uganda<br />

A woman dances in Nampula,<br />

Mozambique<br />

Several transgenic lines of the Gonja<br />

manjaya cultivar will be developed and<br />

evaluated for resistance to BXW in glasshouse<br />

by IITA at the Biosciences East and Central<br />

Africa hub in Kenya. Another CFT at Kawanda,<br />

Uganda is expected to start in mid-2013<br />

and the first one for Kenya in 2014.<br />

Dr Leena Tripathi, Principal<br />

Investigator, BXW Project<br />

Scientists working on the Bacterial Wilt (BXW) Resistant Banana Project<br />

in <strong>2012</strong> reported that they had selected 12 lines out of 65 lines of the<br />

banana cultivar Sukali ndiizi popularly known as apple banana that have<br />

shown resistance to the banana bacterial wilt disease. Sixty-five (65)<br />

lines were tested in confined field trials (CFTs) at Uganda’s National<br />

<strong>Agricultural</strong> Research Organisation’s (NARO) Kawanda research station.<br />

Results from the trials demonstrated that constitutive expression of the<br />

sweet pepper Hrap or Pflp gene in banana resulted in enhanced resistance<br />

to Xanthomonas wilt.<br />

Product development progress<br />

The 12 lines were singled out of 65 transgenic plants and non-transgenic<br />

mother plants that the Project evaluated for BXW resistance and<br />

assessed for disease symptom development in the first CFT in Uganda.<br />

All transgenic lines tested showed a significantly higher resistance in<br />

comparison to control non-transgenic plants. The bunch weight and size<br />

of the transgenic lines were found to be similar to non-transgenic plants.<br />

The best 10 lines will be tested further with more replicates in a second<br />

Deploying agricultural technologies for farmers

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