Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs
Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs
Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs
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<strong>Culture</strong> method<br />
• Strict requirements for <strong>the</strong> culture<br />
medium and growth conditions<br />
(aerobic/anaerobic), generally<br />
requires non-standard adjustments<br />
for <strong>the</strong> <strong>in</strong>dividual id species<br />
• Extremely long test<strong>in</strong>g times of 1 to<br />
4 weeks<br />
• Difficult analysis<br />
• Broth and disk possible<br />
• Advantage: only liv<strong>in</strong>g mycoplasma<br />
is detected<br />
t d<br />
• Sensitivity: 1 CFU corresponds to<br />
average 30 GU<br />
M. arg<strong>in</strong><strong>in</strong>ii<br />
Source: <strong>Mycoplasma</strong> Experience Ltd.<br />
bar = 1 mm