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Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs

Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs

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<strong>Culture</strong> method<br />

• Strict requirements for <strong>the</strong> culture<br />

medium and growth conditions<br />

(aerobic/anaerobic), generally<br />

requires non-standard adjustments<br />

for <strong>the</strong> <strong>in</strong>dividual id species<br />

• Extremely long test<strong>in</strong>g times of 1 to<br />

4 weeks<br />

• Difficult analysis<br />

• Broth and disk possible<br />

• Advantage: only liv<strong>in</strong>g mycoplasma<br />

is detected<br />

t d<br />

• Sensitivity: 1 CFU corresponds to<br />

average 30 GU<br />

M. arg<strong>in</strong><strong>in</strong>ii<br />

Source: <strong>Mycoplasma</strong> Experience Ltd.<br />

bar = 1 mm

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