Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs
Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs
Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Recommendations for Improvement<br />
• Regularly and sensitive test<strong>in</strong>g (monthly); weekly test<strong>in</strong>g <strong>in</strong> cases of<br />
laboratory contam<strong>in</strong>ation<br />
• Operate free of standards antibiotics<br />
• Whenever possible: separate <strong>the</strong> work benches and <strong>in</strong>cubators for handl<strong>in</strong>g<br />
contam<strong>in</strong>ated and mycoplasma free materials<br />
• Never use conta<strong>in</strong>ed cultures; reject immediately or treatedt • Dis<strong>in</strong>fect work<strong>in</strong>g surfaces and hands with alcoholic spray before and after<br />
work<strong>in</strong>g procedures, or with <strong>the</strong> change of <strong>the</strong> work<strong>in</strong>g material<br />
• Quarant<strong>in</strong>e new cells of any orig<strong>in</strong> and <strong>in</strong>tegrate <strong>in</strong>to <strong>the</strong> laboratory only<br />
after test<strong>in</strong>g negative<br />
• For larger loads of serum, <strong>in</strong>cubate on <strong>in</strong>dicator cells 3T3 or Vero over 4<br />
passages before <strong>in</strong>tegration and use, and <strong>the</strong> test supernatants by means of<br />
PCR
Change language