Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs
Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs
Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs
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Effects on <strong>Cell</strong> <strong>Culture</strong><br />
• Inhibition of cell proliferation up to 50% by nutrient withdrawal and secretion<br />
of harmful metabolic products<br />
McGarrity et al. (1984) In Vitro <strong>Cell</strong>. Dev. Biol. 20:1<br />
• fast glucose reduction and formation of acids => p H shift<br />
• arg<strong>in</strong><strong>in</strong>e depletion => <strong>in</strong>hibition of prote<strong>in</strong> biosyn<strong>the</strong>sis, cell division and growth<br />
• Influence of immunological reactions<br />
(macrophage activation, <strong>in</strong>hibition of antigen presentation, ti <strong>in</strong>duction of signal transduction)<br />
ti Mühlradt et al. (1996) Biochemistry 35:7781<br />
• Influence of virus proliferation and <strong>the</strong> <strong>in</strong>fection rate<br />
Nar-Paz et al.(1995) FEMS Microbiol. Lett. 128:63<br />
• Cause chromosomal aberrations and multiple translocations<br />
McGarrity et al. (1978) In: <strong>Mycoplasma</strong> <strong>in</strong>fection of cell cultures. Plenum Press S. 213ff<br />
• Disturbance of <strong>the</strong> hybridoma technique, contam<strong>in</strong>ated cells become<br />
sensitive ii <strong>in</strong> HAT medium
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