Artificial Sweeteners Analysis
Artificial Sweeteners Analysis
Artificial Sweeteners Analysis
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Working Method<br />
HPLC with UV Diode Array Detection<br />
Acesulfame K, Saccharin, Aspartame, NHDC<br />
Column : ACE 5 C18 250 x 4 mm 5µm<br />
Guard column : ACE 5 C18 10 x 4 mm 5µm<br />
Column temperature : 30°C,<br />
Flow :<br />
1.0 mL/min<br />
Stop time : 40 mins<br />
Solvent : Time (min) 0 25 30 35<br />
A : Water 88% 50% 30% 88%<br />
B : Acetonitrile 2% 40% 60% 2%<br />
C : Methanol 0% 0% 0% 0%<br />
D :1% TFA 10% 10% 10% 10%<br />
Injection volume : 5µL<br />
Detector : Acesulfame K 231nm, Saccharin 231nm, Aspartame 215nm,<br />
NHDC 280nm.
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