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Antigen Biotinylated Anti-Rabbit lgs Rabbit Primary Antiserum AB ...

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Light Perception - Material & Methods<br />

The maze was tightly closed against light except of the T-shaped maze part where the<br />

light was positioned (fig. A6). Here, an area of about 5 cm x 5 cm was being left open,<br />

imitating the opening of a tunnel roof (e.g. as happens when a tunnel is damaged or broken<br />

from above by a predator or by mole-rat hunters). Light intensity under this hole was 0.06<br />

µmol<br />

photons<br />

HALOGEN<br />

LAMP<br />

STAND<br />

−2 −1<br />

⋅ m ⋅ s under natural light conditions. The sides of this maze part adjacent to<br />

the opening were covered with thick, black, light impervious paper sheets. White light was<br />

produced by a halogen lamp. This lamp was positioned above the tunnel opening at different<br />

heights (80, 60, 40, 20 and 10 cm) to create diverse light intensities from daylight to sunlight.<br />

DISTANCE<br />

LID<br />

BLOCK<br />

FIBRE<br />

Fig. A6 T-maze for measurements of light propagation in a tunnel. All maze tunnel parts<br />

were 9 x 9 cm in height and width. The main tunnel was 100 cm long, the T-shaped tunnel 120<br />

cm. The tunnels were closed. The white light source, a halogen lamp, was positioned at different<br />

heights over the opening in the T-shaped tunnel’s middle by moving it vertically on a stand.<br />

During these measures, the spectrometer fibre was positioned directly under the lamp. With the<br />

lamp positioned at 10 cm above the tunnel opening, the spectrometer was positioned at different<br />

distances from the junction in the main tunnel, fixated on a block.<br />

21

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