DART-Agilent Vapur Interface Manual SI-300-A Rev 6 ... - IonSense

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Figure 13: Work List Parameters… Menu 4. Select “External Start” from the drop down menu for “Run Type” as shown in Figure 13. Figure 14: Worklist tab for sample queue 5. In the “Worklist” tab set up your sample name, method, data file, and sample type and to begin your run. DART-Agilent Vapur Interface Manual (SI-300-A Rev 6) 14
Calibrating the Agilent TOF with DART Thanks to George Dubay of Duke University and Howard Sanford of Agilent Technologies for their assistance with this section. While a tune done with the ESI source will remain current with the DART, it is necessary to calibrate the Agilent TOF with the DART before usage to ensure mass accuracy. The critical factor for the calibration is ion stability. If the ion current is unstable during calibration, the system destabilizes and the mass accuracy will drift significantly. To ensure a stable ion current: The ions used for calibration must be not too small or large: ~100-700 Da. IonSense recommends PEG-600 as a calibration compound for achieving these target ions. Maintain the instrument vacuum at the lower reaches of 10 -7 . At high vacuum pressures (10 -6 and lower), performance decreases. Also, the vacuum should be relatively constant and not wildly fluctuating. o To do this, it may be necessary to pull the DART source away from the ceramic tube inlet so that the gap between the ceramic cap and ceramic tube is ~8.5cm. o When using IonSense experiment modules to introduce samples, attach them to the rail in the position farther away from the API inlet. Target ion abundances for calibration ions should be in the range of 200- 500K. Greater abundances will lead to system instability. It is recommended to use the Agilent instrument’s standard capillary instead of the resistive-type capillary. DART-Agilent Vapur Interface Manual (SI-300-A Rev 6) 15
Page 1 and 2: DART-Agilent Vapur Interface Manual
Page 3 and 4: This manual details the steps neces
Page 5 and 6: Figure 2: Source mounted - hardware
Page 7 and 8: Attaching the DART Vapur Flange to
Page 9 and 10: Figure 8: Vapur Interface fully mou
Page 11 and 12: Enabling Contact Closure A B Figure
Page 13: MassHunter Data Acquisition: Contac

DART-Agilent Vapur Interface Manual SI-300-A Rev 6 ... - IonSense

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