EQUINE CLINICAL PATHOLOGY - Rossdale & Partners

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T h e B e a u f o r t c o t t a g e l a b o r a t o r i e s sampling requirements Laboratory results are only as good as the samples submitted allow them to be. Samples should be taken by the correct techniques, with suitable equipment, into suitable containers and media/preservatives and securely dispatched to the laboratory in the shortest possible time. Labelling and request forms All specimens should be labelled legibly with the name of the horse, date and time of sampling and site of collection, if appropriate, to enable proper reporting and certification of results. A concise case history should always be included, to help the laboratory make sure that the appropriate tests are performed and reported quickly and efficiently and in order to allow the clinical pathologist to help the clinician interpret the results. Dispatch Avoid sending perishable specimens over the weekend, where they may be held up and deteriorate in the post. Where possible it is sensible to centrifuge or to allow clotted blood samples to stand until it is possible to pour or pipette off the serum, in order to avoid haemolysis. Do not refrigerate EDTA samples or haemolysis will render the results useless. Do not send frozen or unfixed tissues for histological examinations as they will undergo natural autolysis and arrive in a less than suitable or even useless condition for satisfactory examination. For urgent samples and for dead foals, foeti and placentae for postmortem examinations, we strongly recommend personal delivery, e.g. by owner or private courier service. Post and Packaging Please instruct your secretarial staff in the proper packaging of specimens so that disappointments do not occur. Sound packaging is essential to avoid the breakage of containers in the post and the loss of, or damage to samples. Internal and external packing is essential ('Jiffy bags' alone cannot be relied upon). The leakage of pathological specimens can present public health hazards and the Royal Mail may refuse to handle soiled packages. The following notice was published and circulated by the Royal College of Veterinary Surgeons in August 1988:- 1. In general, the despatch of deleterious substances by post is banned by the Post Office. There are however special exemptions for pathological material sent to and from laboratories by veterinary
G u i d e t o e q u i n e c l i n i c a l p a t h o l o g y Sampling Equipment Test Sample Container/anticoagulant/preservative Haematology Whole blood EDTA (lilac Vacutainer or blue Monovette) Plasma fibrinogen Whole blood Sodium citrate (blue Vacutainer or green Monovette) Clinical chemistry, serology, minerals Serum Empty tube (red Vacutainer or brown Monovette) and electrolytes Plasma glucose Whole blood Fluoride/oxalate (grey Vacutainer or yellow Monovette) Plasma progesterone Plasma or serum Lithium heparin (green Vacutainer or orange Monovette) Mare pregnancy Serum or plasma Empty tube (red Vacutainer or brown Monovette) Urinalysis Urine Sterile, empty, leak-proof container Blood selenium or Lithium heparin (green Vacutainer or orange glutathione peroxidase Erythrocytes Monovette) Bacteriology Swabs Amies charcoal transport medium Fluids Blood grow medium Faeces Sterile, leak-proof container Blood Blood grow medium Virology Rotavirus Liquid faeces in sterile leak-proof container Swabs Viral transport medium Parasitology Faeces Sterile, leak-proof container Dermatology Skin scrapings Sterile, leak-proof container Cytology Endometrial and Rolled onto sterile gelatine-coated slides and other smears fixed with ‘smear fix’ or carbowax Peritoneal, pleural, One sample preserved in EDTA, another diluted CSF, synovial fluids, 50:50 in cytospin centrifuge fluid and another tracheal washes undiluted in a sterile leak-proof container and BALs (synovial fluid also in blood grow medium for bacterial culture) Histology General tissue Thin, representative samples fixed in an ample samples volume (at least 10 x) of 10% formol saline Endometrial and Bouin’s fluid testicular biopsies Semen analysis Semen One sample diluted 50:50 in formol citrate and another in an empty, sterile, leak-proof container.
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EQUINE CLINICAL PATHOLOGY - Rossdale & Partners

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