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Processed feed pea and canola meal for blue shrimp diets

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94<br />

( )<br />

L.E. Cruz-Suarez et al.rAquaculture 196 2001 87–104<br />

3. Results<br />

3.1. Stability of the <strong>diets</strong> in seawater<br />

Average loss of a dietary dry matter following 1-h immersion was approximately<br />

11% <strong>for</strong> the <strong>diets</strong> containing <strong>pea</strong> <strong>meal</strong>s Ž Table 3 ., except <strong>for</strong> the whole-extruded <strong>pea</strong> diet<br />

Ž WEX .. This diet <strong>and</strong> the reference <strong>diets</strong> Ž 8 <strong>and</strong> 9.<br />

showed a better water stability. The<br />

extruded <strong>canola</strong> diet Ž CEX.<br />

exhibited significantly lower water stability; however crude<br />

protein loss was comparatively low. The WEX diet showed the lowest crude protein loss<br />

while the protein stability of the remaining <strong>diets</strong> was similar to that of control diet in the<br />

growth trial Ž. 7 . The effects of extrusion on increased starch gelatinization Ž Table 1.<br />

may explain the higher water stability of the diet containing whole-extruded <strong>pea</strong>s.<br />

However, this extrusion effect was not observed <strong>for</strong> dehulled <strong>pea</strong>s: %DML <strong>and</strong> CPL<br />

values submitted to factorial ANOVA showed significant probabilities <strong>for</strong> the rawrextruding<br />

factor Ž Ps0.064 <strong>and</strong> 0.058, respectively ., but not <strong>for</strong> the wholerdehulled one<br />

Ž Ps0.112 <strong>and</strong> 0.254, respectively ..<br />

3.2. Feed intake, growth <strong>and</strong> surÕiÕal<br />

The growth bioassay results <strong>and</strong> ANOVA probabilities <strong>for</strong> the calculated parameters<br />

are presented in Table 4. Highly significant differences Ž P-0.001.<br />

in food intake were<br />

observed. The micronized <strong>pea</strong> <strong>meal</strong> diet WMI showed the highest consumption while<br />

both extruded <strong>pea</strong> <strong>meal</strong> <strong>diets</strong>, WEX <strong>and</strong> DEX showed the lowest intake. The raw <strong>pea</strong><br />

Table 3<br />

Proximate analysis <strong>and</strong> calculated energy content of the experimental <strong>diets</strong>, <strong>and</strong> dry matter loss Ž %DML.<br />

<strong>and</strong><br />

crude protein loss Ž %CPL.<br />

after 1 h immersion in seawater<br />

1 1 1 1 1 3 3<br />

Diet Moisture Crude Lipid Ash Fiber N.F.E Gross %DML %CPL<br />

1<br />

protein<br />

2<br />

energy<br />

1 WRA 7.5 30.6 9.5 8.7 1.4 42.4 4.3 11.2"0.4 b 19.9"3.0 bc<br />

2 WEX 7.5 29.7 8.9 9.0 2.7 42.3 4.2 9.9"1.0 ab 12.6"1.1 a<br />

3 DRA 7.6 30.5 9.6 8.6 0.9 42.8 4.4 11.3"1.0 bc 17.4"3.1 b<br />

4 DEX 7.3 30.8 9.3 8.6 0.9 43.1 4.4 11.1"0.9 b 18.5"1.9 b<br />

5 WMI 7.0 29.6 9.6 8.6 2.4 42.9 4.3 11.2"1.4 b 18.9"1.8 b<br />

6 CEX 7.2 30.2 9.3 8.8 3.8 40.7 4.2 12.6"0.8 c 17.2"1.4 b<br />

7 Control 6.8 31.2 8.9 8.5 1.5 43.2 4.4 11.1"0.5 b 18.9"0.4 b<br />

growth<br />

8 Reference 6.6 35.8 12.8 11.0 1.5 32.3 4.5 9.6"1.0 a 17.3"0.8 b<br />

<strong>pea</strong> <strong>meal</strong><br />

9 Reference 6.7 29.0 11.8 9.1 1.6 41.9 4.5 9.6"0.7 a 19.1"0.6 b<br />

<strong>canola</strong><br />

1 % as fed. NFEsnitrogen free extract, calculated by difference.<br />

2<br />

Proteins5.6 kcalrg, lipids9.5 kcalrg, carbohydratess4.1 kcalrg Ž Tacon, 1987 ..<br />

3 Different letters in same column indicate different homogeneous subsets as determined by Duncan’s<br />

multiple comparisons Ž P s0.05 .. One-way ANOVA probabilities were P s0.001 <strong>and</strong> P s0.006 <strong>for</strong> DML<br />

<strong>and</strong> CPL data, respectively.

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