Identification of Bacterial Pathogens basic skills in bacteriology

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Identification of Microorganisms• For many students and professionals the mostpressing topic in microbiology is how to identifyunknown specimens.• Why is this important?• Labs can grow, isolate and identify most routinelyencountered bacteria within 48 hrs of sampling.• The methods microbiologist use fall into threecategories:♣Phenotypic- morphology (micro andmacroscopic)♣Immunological- serological analysis♣Genotypic- genetic techniquesDr.T.V.Rao MD2
Page 1: Identification of BacterialPathogen
Page 6 and 7: Specimen Collection• Successful i
Page 8: Phenotypic Methods of Identificatio
Page 17 and 18: Gm+ve cocci & Gm-ve bacilliDr.T.V.R
Page 19 and 20: BacilliDr.T.V.Rao MD 19
Page 21 and 22: Wound specimen - Gram stainhttp://w
Page 23 and 24: Sputum specimen Gram stain—Strept
Page 25 and 26: Staining techniques forMycobacteria
Page 27 and 28: How the Acid fast bacteria appearDr
Page 29 and 30: Sputum specimen—Acid fast stainht
Page 31 and 32: Fluorochrome AFB Microscopy* More r
Page 33 and 34: Indirect Fluorescent Antibodies33
Page 35 and 36: Yeast—calcofluor whitehttp://www.
Page 37 and 38: Influenza virus infected cells, flu
Page 39 and 40: Culture and isolation of bacteria
Page 41 and 42: According to Use• Enriched Medium
Page 43 and 44: According to Use• Selective Mediu
Page 45 and 46: Hemolysis a guiding factorDr.T.V.Ra
Page 47 and 48: Enriched MediaChocolate agar, a med
Page 49 and 50: Dr.T.V.Rao MD 49
Page 51 and 52: MacConkey AgarDr.T.V.Rao MD 51
Page 53 and 54:
Differential Media• Differential
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Dr.T.V.Rao MD 55
Page 57 and 58:
• Types of artificial media- Hekt
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• Environmental requirements- Oxy
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http://science.nhmccd.edu/biol/well
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MacConkey agarhttp://science.nhmccd
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• Nutritional requirements and me
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CarbohydrateFermentation . This med
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Nitrate Reduction ♫After 24-48 hr
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Dr.T.V.Rao MD 71
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Coagulase TestDr.T.V.Rao MD 73
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O-F glucose mediahttp://academic.mw
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• Principles of Phenotype-based I
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Rapid TestspositivenegativeONPG (β
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Heavily Inoculated PlateDr.T.V.Rao
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Uncultivable Organisms• Environme
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Flow Cytometry• The cytometer for
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Immunological Methods• Immunologi
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API strips - bioMerieuxDr.T.V.Rao M
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Immunochemical methods for ID• Pr
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Principles of Serologic Test Method
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Genotypic Methods• Genotypic meth
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RAPD-PCR• Random amplified polymo
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Computer and Bacteria Identificatio
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