Identification of Bacterial Pathogens basic skills in bacteriology

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1 2 34 5 67Ziehl-Neelsensta<strong>in</strong>Dr.T.V.Rao MD26
How the Acid fast bacteria appearDr.T.V.Rao MD27
Page 1 and 2: Identification of BacterialPathogen
Page 5 and 6: MicrobeIdentificationDr.T.V.Rao MD5
Page 7 and 8: SpecimenCollectionDr.T.V.Rao MD7
Page 12: Phenotypic Methods• Macroscopic m
Page 18 and 19: StreptococcusDr.T.V.Rao MD 18
Page 20 and 21: Neisseria gonorrhea - Gram stainhtt
Page 22 and 23: Oral specimen - Gram stainDr.T.V.Ra
Page 24 and 25: Sputum—cystic fibrosis patient, e
Page 28 and 29: A Gram stain (left) of the abscess
Page 30 and 31: Acid fast stain demonstrating chord
Page 32 and 33: Direct Fluorescent Antibodies32
Page 34 and 35: Mycobacterium - auramine stainhttp:
Page 36 and 37: Mould—calcofluor whiteDr.T.V.Rao
Page 38 and 39: Better Use Microscopy• Phase Cont
Page 40 and 41: • Media classifications and funct
Page 42 and 43: • Types of artificial media- Brai
Page 44 and 45: According to Use• Differential Me
Page 46 and 47: Hemolysis a guiding factorDr.T.V.Ra
Page 48 and 49: Testing for Bacitracin and Optochin
Page 50 and 51: Mac Conkey Agar a Minimaldifferenti
Page 52 and 53: General vs Selective MediaDr.T.V.Ra
Page 54 and 55: Salmonella-Shigella AgarDr.T.V.Rao
Page 56 and 57: • Types of artificial media- Hekt
Page 58 and 59: Types of artificial media- Thayer-M
Page 60 and 61: • Bacterial Cultivation- Isolatio
Page 62 and 63: Chocolate agarUninoculatedHaemophil
Page 64 and 65: Hektoen agarhttp://medic.med.uth.tm
Page 66 and 67: Biochemical Tests• The microbe is
Page 68 and 69: Methyl Red Test• This is a qualit
Page 70 and 71: Biochemical Tests• Other biochemi
Page 72 and 73: Dr.T.V.Rao MD 72
Page 74 and 75: -Tests for presence ofmetabolic pat
Page 76 and 77:
Chromagar orientationuses colour-fo
Page 78 and 79:
Rapid Tests• Rapid test: a bioche
Page 80 and 81:
Bacteriophage Typing• What are ba
Page 82 and 83:
Bacteriophage Typing• The plate i
Page 84 and 85:
Flow Cytometry• Classical techniq
Page 86 and 87:
Recent Advances in Flowcytometry•
Page 88 and 89:
Principles of Serologic Test Method
Page 90 and 91:
Commercial ID systems- Advantages a
Page 92 and 93:
Serologic methods for diagnosis•
Page 94 and 95:
Genotypic methods• Genotypic meth
Page 96 and 97:
Real Time PCR and RT-PCR• Current
Page 98 and 99:
Plasmid fingerprinting• The proce
Page 100:
• Programme created byDr.T.V.Rao
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Identification of Bacterial Pathogens basic skills in bacteriology

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