Identification of Bacterial Pathogens basic skills in bacteriology
Identification of Bacterial Pathogens basic skills in bacteriology
Identification of Bacterial Pathogens basic skills in bacteriology
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Phenotypic Methods <strong>of</strong> <strong>Identification</strong>• Microbiologists use 5 <strong>basic</strong> techniques to grow,exam<strong>in</strong>e and characterize microorganisms <strong>in</strong>the lab.• They are called the 5 ‘I’s: <strong>in</strong>oculation,<strong>in</strong>cubation, isolation, <strong>in</strong>spection andidentification.• Inoculation: to culture microorganisms a t<strong>in</strong>ysample (<strong>in</strong>oculum) is <strong>in</strong>troduced <strong>in</strong>to medium(<strong>in</strong>oculation).• Isolation <strong>in</strong>volves the separat<strong>in</strong>g one speciesDr.T.V.Rao MDfrom another.8
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