Identification of Bacterial Pathogens basic skills in bacteriology

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Better Use Microscopy• Phase Contrast Microscopy– shift in light allows visualization of organism; canvisualize viable organisms• Fluorescent Microscopy– certain dyes (fluorochromes) give off light whenexcited (fluorescence)– color of light depends on the dye and the filters used– Staining techniques• Fluorochroming: direct chemical interaction with organism– Acridine orange: stains nucleic acid; useful for cell-wall deficientorganisms– Auramine-rhodamine: bind to mycolic acids in nearly allMycobacteria– Calcoflour white: binds to chitin in cell walls of fungi• Immunofluorescence: fluorochrome is bound to an antibody;can detect/identify specific organismsDr.T.V.Rao MD 38
Culture and isolation of bacteria• Principles of Cultivation– Nutritional requirements• General concepts– non-fastidious: simple requirements for growth– fastidious: complex, unusual, or uniquerequirements for growth• Phases of growth media– solid agar; boil to dissolve, solidifies at50ºC– liquid, brothDr.T.V.Rao MD 39
Page 1 and 2: Identification of BacterialPathogen
Page 5 and 6: MicrobeIdentificationDr.T.V.Rao MD5
Page 7 and 8: SpecimenCollectionDr.T.V.Rao MD7
Page 12: Phenotypic Methods• Macroscopic m
Page 18 and 19: StreptococcusDr.T.V.Rao MD 18
Page 20 and 21: Neisseria gonorrhea - Gram stainhtt
Page 22 and 23: Oral specimen - Gram stainDr.T.V.Ra
Page 24 and 25: Sputum—cystic fibrosis patient, e
Page 26 and 27: 1 2 34 5 67Ziehl-NeelsenstainDr.T.V
Page 28 and 29: A Gram stain (left) of the abscess
Page 30 and 31: Acid fast stain demonstrating chord
Page 32 and 33: Direct Fluorescent Antibodies32
Page 34 and 35: Mycobacterium - auramine stainhttp:
Page 36 and 37: Mould—calcofluor whiteDr.T.V.Rao
Page 40 and 41: • Media classifications and funct
Page 42 and 43: • Types of artificial media- Brai
Page 44 and 45: According to Use• Differential Me
Page 46 and 47: Hemolysis a guiding factorDr.T.V.Ra
Page 48 and 49: Testing for Bacitracin and Optochin
Page 50 and 51: Mac Conkey Agar a Minimaldifferenti
Page 52 and 53: General vs Selective MediaDr.T.V.Ra
Page 54 and 55: Salmonella-Shigella AgarDr.T.V.Rao
Page 56 and 57: • Types of artificial media- Hekt
Page 58 and 59: Types of artificial media- Thayer-M
Page 60 and 61: • Bacterial Cultivation- Isolatio
Page 62 and 63: Chocolate agarUninoculatedHaemophil
Page 64 and 65: Hektoen agarhttp://medic.med.uth.tm
Page 66 and 67: Biochemical Tests• The microbe is
Page 68 and 69: Methyl Red Test• This is a qualit
Page 70 and 71: Biochemical Tests• Other biochemi
Page 72 and 73: Dr.T.V.Rao MD 72
Page 74 and 75: -Tests for presence ofmetabolic pat
Page 76 and 77: Chromagar orientationuses colour-fo
Page 78 and 79: Rapid Tests• Rapid test: a bioche
Page 80 and 81: Bacteriophage Typing• What are ba
Page 82 and 83: Bacteriophage Typing• The plate i
Page 84 and 85: Flow Cytometry• Classical techniq
Page 86 and 87: Recent Advances in Flowcytometry•
Page 88 and 89:
Principles of Serologic Test Method
Page 90 and 91:
Commercial ID systems- Advantages a
Page 92 and 93:
Serologic methods for diagnosis•
Page 94 and 95:
Genotypic methods• Genotypic meth
Page 96 and 97:
Real Time PCR and RT-PCR• Current
Page 98 and 99:
Plasmid fingerprinting• The proce
Page 100:
• Programme created byDr.T.V.Rao
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Identification of Bacterial Pathogens basic skills in bacteriology

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