Identification of Bacterial Pathogens basic skills in bacteriology

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• Media classifications and functions–Enrichment• used to enhance growth of specificorganisms–Supportive• support growth of most non-fastidiousorganisms–Selective• contains agents that inhibit the growth of allagents except that being sought (dyes, bilesalts, alcohols, acids, antibiotics)–Differential• contains factor(s) that allow certainorganisms to exhibit different metaboliccharacteristicsDr.T.V.Rao MD 40
According to Use• Enriched Medium –broth or solid, containsrich supply of specialnutrients that promotesgrowth of a particularorganism while notpromoting growth ofother microbes thatmay be present (e.g BAP& chocolate agar)Dr.T.V.Rao MD 41
Page 1 and 2: Identification of BacterialPathogen
Page 5 and 6: MicrobeIdentificationDr.T.V.Rao MD5
Page 7 and 8: SpecimenCollectionDr.T.V.Rao MD7
Page 12: Phenotypic Methods• Macroscopic m
Page 18 and 19: StreptococcusDr.T.V.Rao MD 18
Page 20 and 21: Neisseria gonorrhea - Gram stainhtt
Page 22 and 23: Oral specimen - Gram stainDr.T.V.Ra
Page 24 and 25: Sputum—cystic fibrosis patient, e
Page 26 and 27: 1 2 34 5 67Ziehl-NeelsenstainDr.T.V
Page 28 and 29: A Gram stain (left) of the abscess
Page 30 and 31: Acid fast stain demonstrating chord
Page 32 and 33: Direct Fluorescent Antibodies32
Page 34 and 35: Mycobacterium - auramine stainhttp:
Page 36 and 37: Mould—calcofluor whiteDr.T.V.Rao
Page 38 and 39: Better Use Microscopy• Phase Cont
Page 42 and 43: • Types of artificial media- Brai
Page 44 and 45: According to Use• Differential Me
Page 46 and 47: Hemolysis a guiding factorDr.T.V.Ra
Page 48 and 49: Testing for Bacitracin and Optochin
Page 50 and 51: Mac Conkey Agar a Minimaldifferenti
Page 52 and 53: General vs Selective MediaDr.T.V.Ra
Page 54 and 55: Salmonella-Shigella AgarDr.T.V.Rao
Page 56 and 57: • Types of artificial media- Hekt
Page 58 and 59: Types of artificial media- Thayer-M
Page 60 and 61: • Bacterial Cultivation- Isolatio
Page 62 and 63: Chocolate agarUninoculatedHaemophil
Page 64 and 65: Hektoen agarhttp://medic.med.uth.tm
Page 66 and 67: Biochemical Tests• The microbe is
Page 68 and 69: Methyl Red Test• This is a qualit
Page 70 and 71: Biochemical Tests• Other biochemi
Page 72 and 73: Dr.T.V.Rao MD 72
Page 74 and 75: -Tests for presence ofmetabolic pat
Page 76 and 77: Chromagar orientationuses colour-fo
Page 78 and 79: Rapid Tests• Rapid test: a bioche
Page 80 and 81: Bacteriophage Typing• What are ba
Page 82 and 83: Bacteriophage Typing• The plate i
Page 84 and 85: Flow Cytometry• Classical techniq
Page 86 and 87: Recent Advances in Flowcytometry•
Page 88 and 89: Principles of Serologic Test Method
Page 90 and 91:
Commercial ID systems- Advantages a
Page 92 and 93:
Serologic methods for diagnosis•
Page 94 and 95:
Genotypic methods• Genotypic meth
Page 96 and 97:
Real Time PCR and RT-PCR• Current
Page 98 and 99:
Plasmid fingerprinting• The proce
Page 100:
• Programme created byDr.T.V.Rao
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