Fitting Michaelis-Menten directly to substrate concentration data
Fitting Michaelis-Menten directly to substrate concentration data
Fitting Michaelis-Menten directly to substrate concentration data
- No tags were found...
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
... which give...dSdt= (k 2E T S( )k−1 +k 2k 1+ S= V maxSK m + Sk 1S + E 0 ⇋ E 1k−→2E0 + Pk −1K m = k −1+k 2k 1= <strong>substrate</strong>-enzyme "affinity"V max = k 2 E T = maximal reaction ratedSdt→ V max , S → ∞,S = K m ⇔ dSdt= V max2