New Researches in Biotechnology - Facultatea de Biotehnologii ...

More documents

Recommendations

Info

Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 201116. Vișoiu, E. (2003): Perfecţionarea biotehnologiilor de devirozare şi înmulţire in vitro a viţei de vieîn scopul creşterii valorii biologice a materialului săditor viticol la standardele internaţionale. Ed.Tipnaste Piteşti, pp.43.17. Clark, M.F. and Adams, A.N. (1977): Characteristics of the microplate method of the enzymelinkedimmunosorbent assay for the detection of plant viruses. J. Gen.Virol. Vol. 34, 3: 475-483.18. Svetleva, D., Velcheva, M., Bhowmik, G. (2003): Biotechnology as an useful tool in common bean(Phaseolus vulgaris L.) improvement. Euphytica 131: 189-200.19. Goldsworthy, A. (1997): Electrical stimulation of tissue culture growth and morphogenesis.Agriculture cell report 8: 14.20. Cogălniceanu, G., Radu, M., Fologea, D., Moisoi, N., Brezeanu, A. (1998): Stimulation oftobacco shoot regeneration by alternating weak electric field. Bioelectrochem. Bioenerg. Vol. 44, 2:257-260.21. Köse, C. (2007): Effects of direct electric current on adventitious root formation of a grapevinerootstock. Am. J. Enol. Vitic. Vol. 58, 1:120-123.22. Hormozi-Nejad, M.H., Mozafari, J., Rakhshandehroo, F. (2010): Elimination of Bean commonmosaic virus using an electrotherapy technique. J. Plant Dis. Prot. Vol. 117, 5: 201-205.50
Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011EFFECT OF INORGANIC NITROGEN NUTRITION ON “IN VITRO”PRODUCTION OF POTATO MICROTUBERS*INCDCSZ Braşov, e-mail: nistorandreea06@gmail.comANDREEA NISTOR, NICOLETA CHIRU, CARMEN BĂDĂRĂUAbstract: The effect of inorganic nitrogen nutrition on production of microtubers was studied in twopotato genotypes Nicoleta and Christian. The objective of this study was to investigate whether areduction in total nitrogen level in the Murashige & Skoog medium would improve microtuberization.The effect of three levels of total nitrogen (30, 45 and 60 meq) on tuberization was studied at constant(20 meq K). Reducing the total nitrogen supply increased the number but decreased the size ofmicrotubers. The weight of microtubers per vessel was the highest at the highest nitrogenconcentration (60 meq): 1.91 g for Christian variety. A reduction in total nitrogen supply reduced thesize of microtubers, the lowest weight being at the lowest nitrogen level – 30 meq: 1.01 g for Nicoletavariety, with a difference of -0.66 g, significant in a negative way. Decreasing the total nitrogensupply caused increasing the number of microtubers/ vessel which was the highest at the lowestnitrogen concentration (30 meq): 19.67 microtubers, with a difference of 4.34 microtubers,significant in a positive way, for Christian variety. The number of microtubers per vessel was thelowest at the highest nitrogen concentration (60 meq): 15.33 microtubers, for both varieties.Keywords: microplants, mineral nutrition, microtubers, Solarium tuberosum L., tissue culture1. INTRODUCTIONSome cultivars are more sensitive than others to total nitrogen levels or relativeconcentration of nitrate: ammonium in the medium during micropropagation (Avila et al.1998) or microtuberization (Garner and Blake 1989; Sarkar and Naik 1998). Low nitrogenin the micropropagation and microtuberization media was best for microtuberization(Stallknecht and Farnsworth 1979; Wattimena 1983). Total nitrogen and nitrate:ammonium levels were used effectively to improve preconditioning, induction (Charles etal. 1995; Zarrabeitia et al. 1997; Sarkar and Naik 1998), and microtuber growth (Chen andLiao 1993; Sarkar and Naik 1998). Reduced ammonium levels during source plantpreconditioning resulted in increased numbers and better synchronization of subsequentmicrotuber induction (Charles et al. 1995; Zarrabeitia et al. 1997; Vreugdenhil et al. 1998).The amount of nitrogen had an effect on microtuberization (Cutter, 1978; Moorby,1978).Stallknecht and Farnsworth (1979) and Wattimena (1983) found that low nitrogenin both the explants and the tuberization media was best for induced tuberization in potato.In the absence of growth regulating substances in the medium, a reduction in total nitrogensupply or an increase in the ratio of ammonium to nitrate reduced the size and number ofmicrotubers (Garner & Blake, 1989).Hoque et al., (1996) reported that higher number of microtubers can be producedin MS medium by reducing KN0 3 by half of the total amount. Reduction in the amount ofNH 4 N0 3 to 1/8th of its amount present in MS medium, initiated early and highermicrotuberization after 4 wk (Zarrabeitia et ai., 1997). Nitrogen is one of the major nutrient51
Page 1 and 2: UNIVERSITY OF AGRONOMICAL SCIENCES
Page 3 and 4: Proceeding of the 4 rd Internationa
Page 49: Proceeding of the 4 rd Internationa
Page 101 and 102:
Proceeding of the 4 rd Internationa
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Day 7Day 10Proceeding of the 4 rd I
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
show all

New Researches in Biotechnology - Facultatea de Biotehnologii ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?