Genome Mapping Professor Simon G. Gregory

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Genome MappingProfessor Simon G. GregoryThe human genome in contextBacteriophage φX174 – Sanger et al., 1977Bacteriophage λ – Sanger et al., 1982Mitochondria – Shotgun strategy Anderson et al., 1981S. cerevisiae – Hierarchical strategy Olson et al., 1986C. elegans – Hierarchical strategy Coulson et al., 19863,000,000)Kb (log 10 )1,83097,000125,000120,00012,0004,600485Bacteriophage φX174Bacteriophage λE. coli C. elegans H. sapiensS. cerevisiaeH.influenzae A. thaliana28The human genome project• To determine the sequence of the 3.2 billion basesin the genome• To decode d the sequence in order to extracttthe biological information within• To apply this information to the study of disease,biology and evolution29Human genome project ethos“All human genomic sequence information should be freelyavailable and in the public domain in order to encourageresearch and development and to maximize its benefitto society”The Bermuda Statement, February 1996• Data deposited in public database every 24 hours• No patents are filed30The screen versions of these slides have full details of copyright and acknowledgements10
Genome MappingProfessor Simon G. GregoryThe Wellcome Trust Sanger InstituteWashington University, Genome Sequencing CenterWhitehead Institute for Biomedical Research/MITJoint Genome Institute, U.S. Department of EnergyBaylor College of MedicineGenoscopeInstitute for Molecular Biotechnology, Jena, GermanyRIKEN Genome Sciences CenterUniversity of Oklahoma, Advanced Genome Technology CenterThe Institute for Genome ResearchThe Wellcome TrustUniversity of Washington Genome CenterOther CentresSanger Institute 28.4%University of Washington Multimegabase Sequencing CenterKeio UniversityStanford Human Genome CenterUniversity of Texas, Southwestern Medical CenterJapanese Foundation for Cancer ResearchMax Planck Institute for Molecular GeneticsGenome Therapeutics CorporationCenter for Genetics in Medicine (Perkin Elmer/Washington Univ.)Tokai UniversityCold Spring Harbor LaboratoryGesellschaft fur Biotechnologische Forschung mbHJapan Science and Technology CorporationHuman Genome Center, Institute of Genetics, Chinese Academy of SciencesStanford DNA Sequencing and Technology Development CenterUniversity of Utah Genome CenterCytogenetics and Molecular Genetics, Women's & Children's Hospital, AdelaideThe Yang-Ming University Genome CenterCoordination of humangenome mappingand sequencing 3rdJanuary 2003Baylor College of Medicine9.4%Washington University,Genome 19.9%Whitehead Institutefor BiomedicalResearch/MIT13.5%Joint GenomeInstitutes,U.S. Departmentof Energy11.3%31Hierarchical strategy• Physical (clone) maps, marker based and region / chromosome specificACGTGTCGA3,000 Mb's(3,000,000,000 bp)BAC’s0.1Mb(100 kb)Sequencingsub-clones1.5 kb(1500 bp)Sequence263 Mb(e.g. chromosome 1)32Mapping strategy15 markers/MbRestriction fingerprinting& STS assignment(CA) n (CA) n (CA) nEST EST ESTFS FS FSRestrictionfingerprintingEST15X coverageRPCI 1-6 PACRPCI 11/13 BACRPCI 11/13 BACEST (CA) n (CA) n(CA) n FS EST EST EST FS FS5x draftFinishedAnnotated33The screen versions of these slides have full details of copyright and acknowledgements11
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Genome Mapping Professor Simon G. Gregory

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