Genome Mapping Professor Simon G. Gregory

Genome MappingProfessor Simon G. GregoryUnlinked marker• If a genetic marker is NOT segregating with the diseasein a family, the marker is “unlinked” to the disease12 2212 2212 12 22 22 12 22 22256• Genome-wide linkage analyses - adding up evidencefrom across the families and the genomeparkin intron 7, LOD*=5.0scoreLOD s432• LOD score = A log 10 statistical estimateof whether two loci are likely to lie neareach other on a chromosomeand are therefore likely to be inheritedtogether; A LOD score of 3 or moreis generally taken to indicatethat the two loci are close00 50 100 150 200Map position (cM)26• The generation of a physical or sequence map of an organismfor establishing consensus sequence composition– Hierarchical approach• Physical Map• Sequence generation– Shotgun approachGenome mapping (2)• Whole genome sequence generation– Sequence assembly• Assembly algorithms– Genome annotation• Alignment of cDNA or gene fragments– Genome mapping and sequencing of evolutionary or commerciallyvaluable organisms27The screen versions of these slides have full details of copyright and acknowledgements9