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Table: Half life of Miral 500CS on leaves and fruit, and in soil on two coffeeplantations in Jamaica______________________________________________________________________________Plantation t50 days 95% confidence interval SlopeSample______________________________________________________________________________Mavis Bank (Blue Mountain)Leaves 14.0 52.2 - 239.7 0.740 ± 0.039Berries 3.5 1.0 - 10.6 0.926 ± 0.029Soil 59.8 37.1 - 145.0 1.029 ± 0.038Mountain Hill (Highland)Leaves 2.0 1.0 - 3.2 1.382 ± 0.096Berries 10.0 5.0 - 23.2 1.307 ± 0.048Soil 9.9 5.6 - 19.2 1.281 ± 0.047______________________________________________________________________________ReferencesArnold, D.J. and Briggs, G.G. (1990). The fate of pesticides in soil: predictive and practical aspectsIn: Environmental Fate of Pesticides. (Eds. Hutson, D.H. and Roberts, T.R.), Wiley andSons Ltd. Chichester 7: 101-102.Edwards, C.A. (1975). Factors that affect the persistence of pesticides in plants and soils.Pure andAppl. Chem. 42: 1-2.Hill, I.R. and Wright, S.J.L. (Eds.) (1978) Pesticide microbiology. London, Great Britain;Academic Press Inc. (London) Ltd. 844 pp.Reid, J. C. and D. E. Robinson. (1994). Assessment of different insecticides for the suppressionof an outbreak of the coffee leaf miner, Leucoptera coffeella in Jamaica, W. I. Turrialba.44(1): 112 - 116.O-14 ENGINEERING RESISTANCE TO RINGSPOT POTYVIRUSIN PAPAYAP. Tennant 1 , D. Gonsalves 2 and M. H. Ahmad 11The Biotechnology Centre, Mona;2Department of Plant Pathology, Cornell University, Geneva, NYPapaya ringspot virus (PRSV) is the major disease affecting papaya production in Hawaii, Florida,the Caribbean, South America, India and the Far East. Leaves of infected trees become mottled anddistorted, ringspots appear on fruit, water-soaked streaks appear on stems and petioles and the treebecomes stunted, producing few small fruit.36
In Jamaica, some 40% of papaya orchards have been devastated by PRSV. Since genetic resistanceis not available, efforts were aimed at using transgenic resistance for the control of PRSV inJamaica. Reverse transcription and polymerase chain reaction were used to engineer coat protein(CP) gene constructs of a local PRSV isolate into plant transformation vectors. CP constructs weresubsequently introduced into Carica papaya L. embryogenic calli by microprojectile bombardmentusing NPTII and GUS as selectable markers. Some 150 transgenic lines were obtained from 18bombardment experiments. Under greenhouse conditions, R0 clones of 11 lines proved resistant torepeated manual inoculations with the virus. Similarly inoculated nontransgenic papaya plantsdeveloped symptoms 11 to 40 days after inoculation.Clones of R0 plants have since been transferred to a half-acre plot in Clarendon. Virus infectionwas first observed on nontransgenic plants 6 months after planting using natural aphid populationsas inoculum vectors. Twenty-eight transgenic lines remain symptomless and ELISA-negative.These preliminary results suggest that transgenic resistance could be a practical solution to thePRSV problem in Jamaica.ReferencesGonsalves, D. (1994). Papaya ringspot Virus. In Compendium of Tropical Fruit Diseases. Eds RCPloetz, GA, Zentmyer, WT, Nishijima, KG and Rohrbach, HD Ohr. pp. 67-68. St Paul, MN, APSPress 88p.Yeh, S-D.and Gonsalves, D. (1994). Practices and perspectives of control of papaya ringspot virusby cross protection. In Advances in Disease Vector Research. Ed. KF Harris, 10: 237-257.New York :Springer-Verlag.O-15 GENETIC DIVERSITY AND RELATIONSHIPS AMONGGEMINIVIRUSES FROM JAMAICA AND BARBADOSMarcia E. Roye, Wayne A. McLaughlin, Medhat K. Nakhla and Douglas P. MaxwellDepartment of Basic Medical Sciences-Biochemistry; and The Biotechnology Centre, Mona;Department of Plant Pathology, University of Wisconsin-MadisonGeminiviral DNA probes, polymerase chain reaction and nucleotide sequencing was used to identify7 geminiviruses from Jamaica and 3 from Barbados. PCR-amplified products for DNA-A weregenerated from 80% of plants from Jamaica that gave hybridisation signals and 32% of plants fromBarbados. Nucleotide identity of the common regions of DNA-A and DNA-B, and partial nucleotidesequences of the ac1, av1, bc1, and bv1 genes confirmed the presence of bipartite geminiviruses inthe following crops from Jamaica: P. vulgaris and P. lunatus (bean golden mosaic virus, BGMV-JM2), L. esculentum and C. chinense (tomato dwarf leaf curl virus, TDLCV), and two distinctviruses infecting cabbage. The weeds associated with viruses included Sida spp. (sida golden mosaicvirus, SiGMV-JM), W. amplissima (wissadula golden mosaic virus, WGMV) and three distinctviruses in M.lathyroides (macroptilium golden mosaic virus strains 1 and 2, MacGMV-JM1,MacGMV-JM2 and SiGMV-JM).37
Page 1 and 2: _________________________PROCEEDING
Page 4 and 5: Monday January 11 th 1999__________
Page 6 and 7: FREE ORAL PRESENTATIONSChemistry Le
Page 8 and 9: Wednesday January 13 th , 1999_____
Page 10 and 11: GEOLOGY____________________________
Page 12 and 13: POSTER PRESENTATIONSDisplayed in De
Page 14 and 15: P-20 The compatibility of a thiodan
Page 16 and 17: P-37 Genetic diversity in dasheens
Page 18 and 19: P-55 Trinidad clays as a potential
Page 20 and 21: B. Bark beetles (3 species). The ba
Page 22 and 23: Figure:Population of adult weevils,
Page 24 and 25: O-4 CONDITIONAL ESTIMATION IN AN EX
Page 26 and 27: Six growth forms were observed, all
Page 28 and 29: The difference in coral cover among
Page 30 and 31: Examination of whole and sectioned
Page 32 and 33: Ultimately one of the aims of many
Page 34 and 35: Table:Concentrations of chlorpyrifo
Page 38 and 39: Nucleotide sequence analysis of the
Page 40 and 41: Regardless of the level of fertiliz
Page 42 and 43: Various morphological, physiologica
Page 44 and 45: O-25 HAIMEA ALTA AND HAIMEA OVUMSER
Page 46 and 47: A complete thermodynamic descriptio
Page 48 and 49: One of the promising methods of det
Page 50 and 51: We aim at producing a model which c
Page 52 and 53: in comparison to the function f giv
Page 54 and 55: The scripting language described he
Page 56 and 57: O-36 CRYPTOGRAPHY AND THE INFORMATI
Page 58 and 59: ABSTRACTS OF POSTER PRESENTATIONS__
Page 60 and 61: The varied range of effects to the
Page 62 and 63: P-5 THE USE OF INFORMATION SYSTEMS
Page 64 and 65: The present maximum phytoplankton a
Page 66 and 67: P-9 INDIVIDUAL ZOOPLANKTON SPECIES
Page 68 and 69: The most abundant crustacean captur
Page 70 and 71: Potamic characteristics include slu
Page 72 and 73: Preliminary results have indicated
Page 74 and 75: M. tuberculata was found only at tw
Page 76 and 77: Thiara granifera is gregarious, pro
Page 78 and 79: The population of the CLM in Jamaic
Page 80 and 81: The present studies were undertaken
Page 82 and 83: P-21 ANTIFEEDANT PROPERTIES OF VARI
Page 84 and 85: ReferencesClarke-Harris, D.O. (1998
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Mansingh, A., Robinson, D.E. and Da
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P-24 THE NEW JAMAICA SEISMOGRAPH NE
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P-26 JUPITER LANDSLIDE, RIO GRANDE
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P-27 BIOSTRATIGRAPHIC INVESTIGATION
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P-28 EVALUATION OF MEDIA, METHODS O
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P-31 PHYSIOLOGICAL ECOLOGY OF TEN P
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2. Stavely, J.R., Steadman, J.R. 19
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P-36 IDENTIFICATION, DISTRIBUTION,
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Todd G. A., Daniels M. J. and Callo
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P-42 QUANTIFICATION AND IDENTIFICAT
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P-46 A STUDY OF VIBRIO SPECIES IN R
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P-49 INDOOR LEVELS OF NO2 MEASURED
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Qualitative assessment of these sig
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P-55 TRINIDAD CLAYS AS A POTENTIAL
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The procedure was applied to the Pi
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