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Nucleotide sequence analysis of the common region, ac1 and av1 indicate that geminiviral DNA wasassociated with C. papaya (papaya virus, PaGV).Nucleotide identity of the common regions of DNA-A and DNA-B, and partial nucleotide sequencesof the ac1, av1, bc1, and bv1 genes confirmed the same bipartite geminiviruses (from Barbados) intomato, musk melon and water melon and two distinct geminiviruses in M lathyroides.Nucleotide sequence alignment of the common region and the 5’ end of ac1 was used to determinephylogenetic relationships of the new Jamaican viruses with other Western Hemispheregeminiviruses. Geminivirus isolate from P. vulgaris (BGMV-JM1) and an isolate from P. lunatus(BGMV-JM2) was placed in BGMV type II cluster. SiGMV-JM, and MacGMV-JM1, was closelyrelated to potato yellow mosaic geminivirus (PYMV). The viruses associated with W. amplissima(WGMV), C. papaya (PaGV), M. lathyroides (MacGMV-JM2) and tomato and pepper (TDLCV)were closely related to each other. The tomato virus from Barbados was a new previouslyunreported virus and was distinct from the two macrroptilium viruses. These results indicate thatcrop-infecting and weed-infecting geminiviruses from Jamaica and Barbados are distinct, highlydiverse, and have several geographical origins and weeds are not host to crop-infecting geminivirusesin Jamaica.O-16 TOMATO YELLOW LEAF CURL GEMINIVIRUS INFECTINGHOT PEPPERS (CAPSICUM CHINENSE) IN JAMAICASherline Brown, Conroy Green, Marcia Roye, Wayne McLaughlin,Medhat K. Nakhla and Douglas P. MaxwellDepartment of Basic Medical Sciences (Biochemistry) and The Biotechnology Centre, Mona;Department of Plant Pathology, University of Wisconsin-MadisonSince 1991, farmers in southern and central Jamaica have reported losses of up to 100% in hotpepper (Capsicum chinense) crops, and the causal agent was presumed to be a whitefly-transmittedgeminivirus. Symptoms included mild chlorosis and upward leaf curling. A TYLCV intergenic(specific) DNA probe was used to confirm the presence of TYLCV in 44% of peppers (Capsicumsp) sampled. Polymerase chain reaction (PCR) primers specific for tomato yellow virus (TYLCV-Is) were used to amplify full-length as well as sub-genomic viral fragments from total DNAextracted from pepper (Capsicum sp.) with viral symptoms. These viral fragments were cloned frompepper collected in November 1992 from St. Elizabeth Parish in Jamaica. Nucleotide sequencecomparisons of the intergenic region of the clone from pepper gave >97% identity to TYLCV-Is.Nucleotide sequence identities of the intergenic region among cloned TYLCV intergenic regions fromtomato and pepper infected with TYLCV were >99%. These results indicate that the TYLCVassociated with tomato and pepper plants were all isolates of TYLCV-Is. Electric discharge particleacceleration inoculation of tomato and pepper using a TYLCV full-length clone associated withtomato from Jamaica and Bemisia tabaci transmission studies were used to confirm infectivity ofTYLCV-Is on pepper.Our results suggest that pepper is a non-adapted host for TYLC. This reportis the first to show the association of TYLCV with pepper.38
O-17 POSTHARVEST STORAGE OF YAM TUBERS:A BIOCHEMICAL PERSPECTIVEHelen N. Asemota 1 and Anthony U. Osagie 21Department of Basic Medical Sciences (Biochemistry) and The Biotechnology Centre Mona;2Department of Biochemistry, University of BeninIn this study, healthy yam tubers were kept under local storage conditions and the carbohydratecompositional changes, metabolism of exogenously supplied 1-14 C and 6- 14 C-glucose by the storedtubers were monitored. The changes in the activities with storage of some enzymes of carbohydratemobilization and key enzymes of oxidative pathways were also assayed at regular intervals throughdormancy and sprouting of the stored tubers.Results show remarkable changes in the carbohydrate composition with tuber storage.Chromatographic analysis of the ethanol soluble sugars of non-dormant tubers showed the majorsugars to be glucose, fructose and sucrose.There were also remarkable changes in the activities of enzymes involved in carbohydratemobilization i.e. Phosphorylase, Amylase and Invertase, and carbohydrate oxidation i.e. Glucose6-phosphate dehydrogenase (G6PD) and Phosphofructokinase. The observations on the changes in theactivities of G6PD and PFK during storage, together with the distribution of radioactivity in tuberssupplied 1- 14 C and 6- 14 C glucose, indicate that the predominant pathway of oxidation ofcarbohydrate in stored yam tubers in glycolytic.This study provides evidence that the yam tuber is capable of metabolizing exogenously suppliedglucose and that free pentoses are not accumulated in the stored tubers.The results will be discussed as they relate to biochemical changes in the stored yam tubers, bearingin mind the possibility of producing yam tuber genotype with capacity for early sprouting orprolonged dormancy through genetic transformation.O- 18 IMPROVEMENT OF COMMERCIAL CULTIVATION OFDENDROBIUMS THROUGH SUBSTRATE AND NUTRIENTMODIFICATION: BIOLOGICAL AND ECONOMIC ANALYSISTai Chun, Paulette; Potluri, Sasikala D.P. and Devi Prasad, P.V.Department of Life Sciences, MonaThe commercial cultivation of Dendrobiums has been identified as an area of significant potentialfor the horticulture industry in Jamaica because of its high market demand and price. This crop ismainly cultivated in Thailand and Hawaii in a variety of different growing media and methods ofculture. The present work was undertaken to determine the most suitable and profitable methodsfor the cultivation Dendrobium orchids in Jamaica. A 3 x 3 Latin Square design with split plotswas used to evaluate the performance of Dendrobiums in 7 different media/ culture treatments . Themedia employed were coconut husk shells, coconut husk pads , coconut husk, river gravel, brickchips, igneous rock in clay and plastic pots as well culture without any media. Three levels ofnitrogen were employed - 136 or 272 or 544 Kg N hā 1 yr -1 . Data were collected for two years.39
Page 1 and 2: _________________________PROCEEDING
Page 4 and 5: Monday January 11 th 1999__________
Page 6 and 7: FREE ORAL PRESENTATIONSChemistry Le
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Page 14 and 15: P-20 The compatibility of a thiodan
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Page 18 and 19: P-55 Trinidad clays as a potential
Page 20 and 21: B. Bark beetles (3 species). The ba
Page 22 and 23: Figure:Population of adult weevils,
Page 24 and 25: O-4 CONDITIONAL ESTIMATION IN AN EX
Page 26 and 27: Six growth forms were observed, all
Page 28 and 29: The difference in coral cover among
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Page 34 and 35: Table:Concentrations of chlorpyrifo
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Page 40 and 41: Regardless of the level of fertiliz
Page 42 and 43: Various morphological, physiologica
Page 44 and 45: O-25 HAIMEA ALTA AND HAIMEA OVUMSER
Page 46 and 47: A complete thermodynamic descriptio
Page 48 and 49: One of the promising methods of det
Page 50 and 51: We aim at producing a model which c
Page 52 and 53: in comparison to the function f giv
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Page 56 and 57: O-36 CRYPTOGRAPHY AND THE INFORMATI
Page 58 and 59: ABSTRACTS OF POSTER PRESENTATIONS__
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Page 62 and 63: P-5 THE USE OF INFORMATION SYSTEMS
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Page 72 and 73: Preliminary results have indicated
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Page 78 and 79: The population of the CLM in Jamaic
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Page 84 and 85: ReferencesClarke-Harris, D.O. (1998
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P-24 THE NEW JAMAICA SEISMOGRAPH NE
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P-26 JUPITER LANDSLIDE, RIO GRANDE
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P-27 BIOSTRATIGRAPHIC INVESTIGATION
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P-28 EVALUATION OF MEDIA, METHODS O
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P-31 PHYSIOLOGICAL ECOLOGY OF TEN P
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2. Stavely, J.R., Steadman, J.R. 19
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P-36 IDENTIFICATION, DISTRIBUTION,
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Todd G. A., Daniels M. J. and Callo
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P-42 QUANTIFICATION AND IDENTIFICAT
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P-46 A STUDY OF VIBRIO SPECIES IN R
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P-49 INDOOR LEVELS OF NO2 MEASURED
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Qualitative assessment of these sig
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P-55 TRINIDAD CLAYS AS A POTENTIAL
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The procedure was applied to the Pi
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