8.000.5051EN - Metrohm

Ion chromatographyIon chromatography (IC) is a standard method for wineanalysis. Numerous major and minor compounds thataffect taste and nutrition as well as traces of conta minantscan be reliably and precisely determined by IC.Multicomponent determinations in a single analysisIn addition to anions and cations, sugar compounds, or -ga nic acids and polar substances can also be quantifiedin a variety of wines. The advantage of ion chromatographyis that chemically similar substances can be determinedsimultaneously in a single analysis. Of course, allMetrohm IC instruments and the MagIC Net TM chromatographysoftware comply with FDA regulations.Application exampleAnalysis of acids in wineAcids are an important component in wine. They preservethe wine and give it expression and character. Winecontains several kinds of acids. The most important onesare tartaric acid, malic acid, and lactic acids, as well ascar bo nic acid in sparkling wines.Analysis of biogenic amines in wineApart from the various acids, it is also possible to detectthe biogenic amines produced during malolactic fermentation,e.g., histamine, tryramine, putrescine, and phenylethylamine,with ion chromatography.09181614Conductivity [µS/cm]12108642chloridesuccinatemalatesulfitetartratesulfate00 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18Time [min]19 20Anion and organic acid analysis in wine: column: Metrosep A Supp 10 - 100/4.0;eluent: 5.0 mmol/L Na 2CO 3+ 5.0 mmol/L NaHCO 3, 1.0 mL/min; sample volume: 20 μL,inline dilution: 1:10Inline Eluent PreparationThe time-consuming manual preparation of eluents is athing of the past. Automatic inline preparation of eluentscan be easily done by expanding the IC system with anadditional Dosino and a Level Control. While the LevelControl mo ni tors the eluent level, the Dosino performsall dosing and liquid handling tasks.