data sheet NanoLC-Ultra™ system - Eksigent

Key advantages of the new NanoLC-UltraThe NanoLC-Ultra system is a proteomics lab workhorse,delivering stable, reproducible gradient separations forpeptide/protein identification, phosphopeptide analysis,biomarker discovery, metabolomics and more.Splitless, nano-scale flow controlEksigent’s Microfluidic Flow Control (MFC) technology (seeFigure 1) uses fast flow measurement in combination withautomatic feedback to a regulated pressure source to generateaccurate and reproducible flow rates with a resolution betterthan 1 nl/min. By combining true microfluidic pump controlwith direct feedback of flow rate, the system eliminates theflow inaccuracies and plumbing problems caused by splitterbasedsystems. The result is extremely reproducible gradientsdown to 50 nl/min, without the use of flow splitting, andobserved gradient reproducibility typically better than 0.5%RSD (see Figure 2). And the self-priming/self-purging designmakes the NanoLC-Ultra pump even easier to set-up and run.10,000 psi maximum pressureUltra high pressure LC has been successfully applied in smallmolecule analyses using 2.1 mm ID columns for increasedseparation efficiencies and/or analysis speed. For theidentification of proteins using LC-MS in proteomics, NanoLCis required because of the increased sensitivity obtained bynanospray mass spectrometry.Figure 1. Microfluidic Flow ControlWith MFC technology, direct pumping is used to deliveraccurate, repeatable gradients. Each pressure sourcedelivers fluid with an actual flow rate monitored using theflow modules in each channel. A CPU closes the loop bymaking any necessary adjustments to the flow rate usingelectro-pneumatic controllers.The operating pressure of 10,000 psi obtained with theNanoLC-Ultra system enables the use of longer columns,or columns packed with smaller particles, for the analysis ofincreasingly complex proteomics samples (see Figure 3).Retention time reproducibilityMaintaining a constant column temperature is requisite toobtaining the best possible retention time reproducibility.To address this requirement, NanoLC-Ultra systems areequipped with a temperature-controlled column compartmentwith integrated 10-port valves, that can be regulated to atemperature up to 40°C.Elevated temperatures also can be utilized to decrease columnpressure and/or improve separation efficiency. For advancedapplications like sample pre-concentration or dual-column setup,the NanoLC-Ultra system offers up to two 10-port nanovalves rated at 10,000 psi that are integrated into the columncompartment. These 10-port valves have an extremely low portto-portvolume of