Three Methods for Shading Correction on the ... - UT Southwestern
Three Methods for Shading Correction on the ... - UT Southwestern
Three Methods for Shading Correction on the ... - UT Southwestern
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Method 3: Low pass filtering • If you d<strong>on</strong>’t have a flat field image <str<strong>on</strong>g>for</str<strong>on</strong>g> your images that need to be corrected you can use <strong>the</strong> FFT Bandpass functi<strong>on</strong> in ImageJ to correct <str<strong>on</strong>g>for</str<strong>on</strong>g> shading. This is less ideal but still acceptable in most cases. • Process > FFT > Bandpass • Filter large objects down to 100 pixels: