Protocols for field and laboratory rodent studies - HAL

More documents

Recommendations

Info

Citation:Herbreteau V., Jittapalapong S., Rerkamnuaychoke W., Chaval Y., Cosson J.-F. and Morand S. (Editors). 2011. Protocols forfield and laboratory rodent studies. Retrieved from CERoPath project:http://www.ceropath.org/FichiersComplementaires/Herbreteau_Rodents_protocols_2011.pdfFront cover picture: Bandicota indica, Kalasin, Thailand, © Herbreteau V., 2006Back cover picture: Tracks of Bandicota indica, Kalasin, Thailand, © Herbreteau V., 2006
Foreword“Protocols for field and laboratory rodent studies” is a publication generated by the CERoPath project(Community Ecology of Rodents and their Pathogens in a changing environment, www.ceropath.org) funded bythe French Ministry of Research from 2008 to 2011 (French ANR Biodiversity ANR 07 BDIV 012). This book waswritten and compiled by the various partners directly involved either in the trapping of rodents or in the analyses ofsamples for rodent or macro- and micro-parasites identification.These protocols summarize the techniques that we developed while studying wild rodents, during almost tenyears. It aims to providing accurate and practical knowledge and advice for future research on rodents,keeping in mind that this information would have been precious to all of us when we started our studies.Requirements for trapping and manipulating rodents differ regarding the objectives of the studies. Someobservations can be made without capturing animals: by identification of foot tracks, feces, damage to crops orobjects, burrowing activity, by using camera or video traps, by recording acoustic signatures with ultrasoundsdetectors, etc. These techniques have enabled identification of unknown species and allowed collection ofinformation on the ecology of species (seasonal migrations, daily ranges, density). However trapping may benecessary in some cases. First to describe a new species, some invasive samples (such as hairs) can be takenfor genetic analysis and some measurements can be performed on a live rodent lightly anaesthetized. Second, fora medical survey, a small volume of blood can be obtained using methods that minimize stress and pain for theanimal. Third, longitudinal ecological studies may also require live trapping to mark (ear tag, toe clip, etc.) orfollow (radio transmitter, GPS collar) animals. We placed emphasis on providing colorful illustrations for a betterinterpretation. Consequently, these protocols show some pictures of dead animals since sacrifice is necessary forsome specific research purposes. Nevertheless we tried to present alternatives to sacrifice wherever possible.We have learnt everyday in the field and these protocols have evolved with our different research projects and theexperience we have gained from various collaborations. The list of co-authors and institutions is long buteveryone has played a part in this publication.Some South-East Asian rodents (Photos: Herbreteau V.)Bandicota indica Berylmys berdmorei Chiropodomys gliroidesLeopoldamys neilli Maxomys surifer Mus cookiiIII
Page 3: Protocols for field and laboratory
Page 9: ContentsForeword ..................
Page 12 and 13: Protocols for field and laboratory
Page 24 and 25: INTERNAL SAMPLESBLOODProtocols for
Page 26 and 27: DefinitionsProtocols for field and
Page 28 and 29: Definitions3- Identifying rodents3.
Page 32 and 33: DefinitionsProtocols for field and
Page 40 and 41: Definitions4- Isolating helminths i
Page 44 and 45: 5- Preparing rodents for collection
Page 52 and 53: LocationEnvironmentHumanpresenceTra
Page 54:
DatesMeasurementsSamplesProtocols f
show all

Protocols for field and laboratory rodent studies - HAL

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?