Protocols for field and laboratory rodent studies - HAL

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LocationEnvironmentHumanpresenceTraps / CapturesProtocols for field and laboratory rodent studiesSAMPLING SITEWritten by:Date:Id_Site: Method: Trapped by project LineIsolated trapCollected from localsWho: ………………Administrative: Province: District:Sub-district:Village:GPS coordinatesWGS 1984Decimal degrees Lat: …………………… Long: …………………..UTM/UPS Easting: ……………… Northing: ………………Elevation:……… metersSamplingplace /TypologyComment:Low Medium HighAt thesamplingpoint:Site “nickname”:Other comments:Surroundinglandscape:Type:Closest village………………………………Distance:For distances, use the following codification, based on decimal logarithm (base 10 logarithm): Code = log 10(distance): 0 = 1 m 1 = 10 m 2 = 100 m 3 = 1 km 4 = 10 km 5 = 100 kmHouses: Yes NoNumber:Distance from sampling point:DatelostTrapsreplaced remainingCaptures / remarks44
OrgansDatesOriginMeasuresBloodMaleEctoparasitesSamplingplace /TypologyIn ethanolFemaleAppendicesRODENT SAMPLINGDissected by:Written by:Id_individual:Id_Site:Species:Capture Death Dissection…… / …… / ……… …… / …… / ……… …… / …… / ………Trapped by CERoPath Alive Location accuracy:Collected from: ………………… Dead (1 High – 5 Low)Low Medium HighComment:Weight (g) Head & Body Tail Hind Foot Ear Skull Spleen (mg)Brush:Claw:TestesVaginaTeatsInside Outside Not openTestes length (mm) : ……OpenBarely visibleProminentSeminal vesicles Mammae formula EmbryosUndeveloped Developed …… + …… + …… Left: …… Right: ……Blotting Paper Whole blood Serum Toxoplasmosis / TrypanosomiasisYesNoYesNoYes,NoNb: …Citrate (blue) for PCR(red) for serum(purple) for general morphologyLung(blue)Spleen(white)Kidney(yellow)Collection DNA Diaphragm GutFrozen:RNA later:Nb:…Nb:…Nb:…Nb:…Nb:…Nb:…BodySkullLiverFootOther: ……YesNoYesNoChigger mites Ticks Mites Fleas Lice OthersFrozenFrozenFrozenFrozenFrozenIn ethanolIn ethanolIn ethanolIn ethanolIn ethanolPictures:Comments:45
Page 3 and 4: Protocols for field and laboratory
Page 5: Foreword“Protocols for field and
Page 9: ContentsForeword ..................
Page 24 and 25: INTERNAL SAMPLESBLOODProtocols for
Page 26 and 27: DefinitionsProtocols for field and
Page 28 and 29: Definitions3- Identifying rodents3.
Page 32 and 33: DefinitionsProtocols for field and
Page 40 and 41: Definitions4- Isolating helminths i
Page 44 and 45: 5- Preparing rodents for collection
Page 54: DatesMeasurementsSamplesProtocols f

Protocols for field and laboratory rodent studies - HAL

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