pGL3 Luciferase Reporter Vectors Technical Manual #TM033

VIII.G. pGL3-Basic Vector Restriction Sites (continued)Table 6. Restriction Enzymes That Cut the pGL3-Basic Vector 6 or More Times.AciIAluIBanIBbvIBsaOIBsaJIBsp1286IBsrIBsrSIBst71IBstOIBstUICfoICfr10IDdeIDpnIDpnIIEarIFnu4HIFokIHaeIIHaeIIIHgaIHhaIHinfIHpaIIHphIHsp92IIMaeIMaeIIMaeIIIMboIMboIIMnlIMseIMspIMspA1INciINdeIINlaIIINlaIVPleIRsaISau3AISau96IScrFISfaNITaqITfiITru9IXhoIIVIII.H. pGL3-Enhancer Vector Restriction SitesThe following restriction enzyme tables were constructed using DNASTAR ®sequence analysis software. Please note that we have not verified thisinformation by restriction digestion with each enzyme listed. The locationgiven specifies the 3´ end of the cut DNA (the base to the left of the cut site).For more information on the cut sites of these enzymes, or if you identify adiscrepancy, please contact your local Promega Branch or Distributor. In theU.S., contact Promega Technical Services at 800-356-9526. Vector sequences arealso available in the GenBank ® database (GenBank ® /EMBL Accession NumberU47297) and on the Internet at: www.promega.com/vectors/Table 7. Restriction Enzymes that cut the pGL3-Enhancer Vector Between 1 and 5Times.Enzyme # of Sites LocationAccI 1 2257AccIII 2 783,1299Acc65I 1 1AcyI 4 95, 121, 1514,3936AflIII 3 15, 581 ,2506Alw26I 5 1111, 1343, 1409,3460, 4236Alw44I 2 2820, 4066AlwNI 1 2922AspHI 5 11, 1553, 2824,3985, 4070AvaI 3 26, 32, 1144AvaII 3 1267, 3537, 3759BamHI 1 2250BanII 4 11, 33, 1112, 4477Enzyme # of Sites LocationBbeI 1 124BbsI 4 98, 1376, 1492,2335BbuI 3 751, 2108, 2180BclI 1 668BglI 2 3519, 4787BglII 1 36BsaI 1 3460BsaAI 1 4548BsaBI 1 2003BsaHI 4 95, 121, 1514,3936BsaMI 3 60, 1823, 1916BsmI 3 60, 1823, 1916BspHI 3 671, 3226, 4234BspMI 3 1477, 1486, 5027Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USAToll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.comPart# TM033Printed in USA.Page 20 Revised 9/07