pGL3 Luciferase Reporter Vectors Technical Manual #TM033

11.07.2015 • Views

Share Embed Flag

pGL3 Luciferase Reporter Vectors Technical Manual #TM033

pGL3 Luciferase Reporter Vectors Technical Manual #TM033

DOWNLOAD ePAPER

synthesisgene.com

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

START NOW

VIII.H. <strong>pGL3</strong>-Enhancer Vector Restriction Sites (continued)Table 8. Restriction Enzymes That Do Not Cut the <strong>pGL3</strong>-Enhancer Vector.AatIIAccB7IAflIIAgeIApaIAscIAvrIIBalIBbrPIBlpIBpu1102IBsp120IBssHIIBst1107IBst98IBstEIIBstXIBsu36ICspIEco72IEco81IEcoRIEcoRVI-PpoINdeINruIPacIPflMIPinAIPmeIPmlIPstIPvuIIRsrIISacIISfiISgfISnaBISpeISplISse8387IStuISwaITth111ITable 9. Restriction Enzymes That Cut the <strong>pGL3</strong>-Enhancer Vector 6 or MoreTimes.AciIAluIBanIBbvIBsaOIBsaJIBsp1286IBsrIBsrSIBst71IBstOIBstUICfoICfr10IDdeIDpnIDpnIIEarIFnu4HIFokIHaeIIHaeIIIHgaIHhaIHinfIHpaIIHphIHsp92IIMaeIMaeIIMaeIIIMboIMboIIMnlIMseIMspIMspA1INciINdeIINlaIIINlaIVPleIRsaISau3AINote: The enzymes listed in boldface type are available from Promega.Sau96IScrFISfaNITaqITfiITru9IXhoIIPromega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USAToll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.comPart# TM033Printed in USA.Page 22 Revised 9/07

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
17
18
19
21
23
25
26
27
29
30

EMBL Protein Expression and Purification Facility

pBAD/Myc - Gene Synthesis

pET 40+ - UQ Protein Expression Facility

pUC57 sequence - Gene Synthesis

pET-11a-d Vectors

pET 26+

pBV220 Vector - Gene Synthesis

CAPS-Reagent Grade - Gene Synthesis

puc18 sequence - Gene Synthesis

pEGFP-C1 - Gene Synthesis

pET-16b Vector

pTriEx-4 Neo Vector - Gene Synthesis

Tris-HCl - Gene Synthesis

pET-17xb Vector - Gene Synthesis

VIII.H. <strong>pGL3</strong>-Enhancer Vector Restriction Sites (continued)Table 8. Restriction Enzymes That Do Not Cut the <strong>pGL3</strong>-Enhancer Vector.AatIIAccB7IAflIIAgeIApaIAscIAvrIIBalIBbrPIBlpIBpu1102IBsp120IBssHIIBst1107IBst98IBstEIIBstXIBsu36ICspIEco72IEco81IEcoRIEcoRVI-PpoINdeINruIPacIPflMIPinAIPmeIPmlIPstIPvuIIRsrIISacIISfiISgfISnaBISpeISplISse8387IStuISwaITth111ITable 9. Restriction Enzymes That Cut the <strong>pGL3</strong>-Enhancer Vector 6 or MoreTimes.AciIAluIBanIBbvIBsaOIBsaJIBsp1286IBsrIBsrSIBst71IBstOIBstUICfoICfr10IDdeIDpnIDpnIIEarIFnu4HIFokIHaeIIHaeIIIHgaIHhaIHinfIHpaIIHphIHsp92IIMaeIMaeIIMaeIIIMboIMboIIMnlIMseIMspIMspA1INciINdeIINlaIIINlaIVPleIRsaISau3AINote: The enzymes listed in boldface type are available from Promega.Sau96IScrFISfaNITaqITfiITru9IXhoIIPromega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USAToll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.comPart# TM033Printed in USA.Page 22 Revised 9/07

Page 1 and 2: Technical ManualpGL3 Luciferase Rep
Page 3 and 4: I. DescriptionThe pGL3 Luciferase R
Page 5 and 6: III.B. pGL3-Enhancer VectorThe pGL3
Page 7 and 8: III.D. pGL3-Control VectorThe pGL3-
Page 9 and 10: IV.B. Preparation of pGL3 Vectors a
Page 11 and 12: VI.Assay of Luciferase Activity (co
Page 13 and 14: VIII. AppendixVIII.A. Common Struct
Page 15: VIII.C. The pGL3 Vectors luc+ GeneM
Page 18 and 19: VIII.F. References1. Sambrook, J. e
Page 21: VIII.G. pGL3-Basic Vector Restricti
Page 26 and 27: Table 12. Restriction Enzymes That
Page 29 and 30: VIII.J. pGL3-Control Vector Restric