pGL3 Luciferase Reporter Vectors Technical Manual #TM033

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Table 12. Restriction Enzymes That Cut the pGL3-Promoter Vector 6 or MoreTimes.AciIAluIBanIBbvIBsaOIBsaJIBsp1286IBsrIBsrSIBst71IBstOIBstUICfoICfr10IDdeIDpnIDpnIIEarIFnu4HIFokIHaeIIHaeIIIHgaIHhaIHinfIHpaIIHphIHsp92IIMaeIMaeIIMaeIIIMboIMboIIMnlIMseIMspIMspA1INciINdeIINlaIIINlaIVPleIRsaISau3AISau96IScrFISfaNITaqITfiITru9IXhoIINote: The enzymes listed in boldface type are available from Promega.Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USAToll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.comPrinted in USA.Part# TM033Revised 9/07 Page 25
VIII.J. pGL3-Control Vector Restriction SitesThe following restriction enzyme tables were constructed using DNASTAR ®sequence analysis software. Please note that this information has not beenverified by restriction digestion with each enzyme listed. The location givenspecifies the 3´ end of the cut DNA (the base to the left of the cut site). Formore information on the cut sites of these enzymes, or if you identify adiscrepancy, please contact your local Promega Branch or Distributor. Vectorsequences are also available in the GenBank ® database (GenBank ® /EMBLAccession Number U47296) and on the Internet at:www.promega.com/vectors/Table 13. Restriction Enzymes That Cut the pGL3-Control Vector Between 1 and 5Times.Enzyme # of Sites LocationAccI 1 2449AccIII 2 975, 1491Acc65I 1 1AcyI 4 287, 313, 1706,4128AflIII 3 15, 773, 2698Alw26I 5 1303, 1535, 1601,3652, 4428Alw44I 2 3012, 4258AlwNI 1 3114AspHI 5 11, 1745, 3016,4177, 4262AvaI 3 26, 32, 1336AvaII 3 1459, 3729, 3951AvrII 1 229BamHI 1 2442BanII 4 11, 33, 1304, 4669BbeI 1 316BbsI 4 290, 1568, 1684,2527BbuI 3 943, 2300, 2372BclI 1 860BglI 3 182, 3711, 4979BglII 1 36BsaI 1 3652BsaAI 1 4740BsaBI 2 48, 2195BsaHI 4 287, 313, 1706,4128BsaMI 3 252, 2015, 2108BsmI 3 252, 2015, 2108BspHI 3 863, 3418, 4426Enzyme # of Sites LocationBspMI 3 1669, 1678, 5219BsrGI 1 770BssSI 2 2871, 4255BstZI 3 1947, 1951, 5089ClaI 3 2189, 5147, 5251Csp45I 1 449DraI 4 2155, 3457, 3476,4168DraII 1 1459DraIII 1 4743DrdI 3 1681, 2806, 4787DsaI 2 278, 650EaeI 4 1947, 1951, 3979,5089EagI 3 1947, 1951, 5089EclHKI 1 3591Eco47III 1 2574Eco52I 3 1947, 1951, 5089EcoICRI 1 9EcoNI 3 837, 1237, 1897EheI 1 314FseI 1 1953FspI 2 3813, 4986HincII 3 1584, 2094, 2450HindII 3 1584, 2094, 2450HindIII 1 245HpaI 1 2094Hsp92I 4 287, 313, 1706,4128KasI 1 312KpnI 1 5MluI 1 15Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USAToll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.comPart# TM033Printed in USA.Page 26 Revised 9/07
Page 1 and 2: Technical ManualpGL3 Luciferase Rep
Page 3 and 4: I. DescriptionThe pGL3 Luciferase R
Page 5 and 6: III.B. pGL3-Enhancer VectorThe pGL3
Page 7 and 8: III.D. pGL3-Control VectorThe pGL3-
Page 9 and 10: IV.B. Preparation of pGL3 Vectors a
Page 11 and 12: VI.Assay of Luciferase Activity (co
Page 13 and 14: VIII. AppendixVIII.A. Common Struct
Page 15: VIII.C. The pGL3 Vectors luc+ GeneM
Page 18 and 19: VIII.F. References1. Sambrook, J. e
Page 21: VIII.G. pGL3-Basic Vector Restricti
Page 25: VIII.I. pGL3-Promoter Vector Restri
Page 30: pGL4 Luciferase VectorsProduct Size

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