pGL3 Luciferase Reporter Vectors Technical Manual #TM033

I. DescriptionThe pGL3 Luciferase Reporter Vectors (a,b) provide a basis for the quantitativeanalysis of factors that potentially regulate mammalian gene expression. Thesefactors may be cis-acting, such as promoters and enhancers, or trans-acting,such as various DNA-binding factors. The backbone of the pGL3 LuciferaseReporter Vectors is designed for increased expression, and contains a modifiedcoding region for firefly (Photinus pyralis) luciferase that has been optimized formonitoring transcriptional activity in transfected eukaryotic cells. The assay ofthis genetic reporter is rapid, sensitive and quantitative. In addition, theseLuciferase Reporter Vectors contain numerous features aiding in the structuralcharacterization of the putative regulatory sequences under investigation.II.Product Components and Storage ConditionsProduct Size Cat.#pGL3-Control Vector 20µg E1741pGL3-Basic Vector 20µg E1751pGL3-Promoter Vector 20µg E1761pGL3-Enhancer Vector 20µg E1771Information on related products, including the Luciferase Assay System, is provided inSections IV–VI and VIII.K.Storage Conditions: Store the pGL3 Luciferase Reporter Vectors at –20°C.III.pGL3 Vector Maps and Sequence Reference PointsThe listings of restriction sites for the pGL3 Luciferase Reporter Vectors areprovided in Section VIII.G–J.Note: The specific transcriptional characteristics of the pGL3 Vectors willvary for different cell types. This may be particularly true for COS cells,which contain the SV40 large T antigen. The SV40 large T antigen promotesreplication from the SV40 origin, which is found in the promoter of thepGL3-Promoter and pGL3-Control Vectors. The combination of large T antigenand SV40 origin will result in a higher copy number of these vectors in COScells, which in turn may result in increased expression of the reporter genecompared to other cell and vector combinations.Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USAToll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.comPart# TM033Printed in USA.Page 2 Revised 9/07