Liquid Handling

Graduated PipettesGraduated PipettesIn the DIN EN ISO 835 standard, thewaiting time for graduated pipetteswas reduced from 15 to 5 secondsand the type 2, total delivery, nominalvolume at top, was included.We recommend the graduatedpipette type 2 as with these pipettesthe meniscus needs to be set onlyonce to achieve exact pipetting.In contrast, with types 1 and 3, thereis a risk of over-dispensing while settingthe meniscus for partial volumesthe second time. In such a case, thesample would have to be preparedagain.BLAUBRAND ® Graduated Pipettes, Class ASType 2totaldeliverynominal volumeat the topType 1partialdeliveryzero pointat the topType 3totaldeliveryzero pointat the topVolumetric InstrumentsPrecision pipette tipOptimized flow geometry in the tipensures reliable liquiddispensing from bulband graduated pipettes.The mechanical resilienceof the tip andits fire-polished edgesensure a long servicelife.Setmeniscus1 timeSetmeniscus2 timesSetmeniscus2 timesTechnical data, available sizesGraduated pipettes are calibrated to contain(TC, In) respectively to deliver (TD, Ex).Capacitymlwww.brand.deSubdiv.mlColor-Code(ISO 1769)Length mm(± 10 mm)BLAUBRAND ®waiting time 5 sCalibr.Error limit± mlSILBERBRANDno waiting timeCalibr.Error limit± ml0.1 0.001 2 x green 360 In 0.001 – –0.2 0.002 2 x white 360 In 0.002 – –0.5 0.01 2 x yellow 360 Ex 0.006 Ex 0.0081 0.01 yellow 360 Ex 0.007 Ex 0.0101 0.1 red 360 Ex 0.007 Ex 0.0102 0.01 2 x white 360 Ex 0.010 Ex 0.0152 0.02 black 360 Ex 0.010 Ex 0.0152 0.1 green 360 Ex 0.010 Ex 0.0155 0.05 red 360 Ex 0.030 Ex 0.055 0.1 blue 360 Ex 0.030 Ex 0.0510 0.1 orange 360 Ex 0.05 Ex 0.0820 0.1 2 x yellow 360 Ex 0.1 Ex 0.1525 0.1 white 450 Ex 0.1 Ex 0.1550 0.5 – 450 Ex 0.2 – –DIN EN ISO 835In the DIN EN ISO 835,the waiting time forgraduated pipettes ofclass AS was reducedfrom 15 to 5 seconds.All BLAUBRAND ® graduated pipettesare supplied with a batch number andan accompanying batch certificate inthe original packaging.On request, they are also availablewith an individual certificate, USP individualcertificate or DAkkS calibrationcertificate (ordering information,page 173).177