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8. Cytology kit – Cytoset.8.1. Intended Use.The kit for cytological purposes “Cytoset” is standard equipment for the <strong>MPW</strong>-223c centrifugemanufactured by <strong>MPW</strong> Med. instruments. Its intended use is the centrifugal separation of body fluids intofine subconstituent elements from the filtrate. The fine elements are deposited directly on basic microscopeslides, while the filtrate is accumulated in special 2.2 ml tubes, following the settling of the particulatematter. The tested physiological fluids constitute a suspension of fine elements in the fluid fraction.Among them are the following:a) natural biological fluids including: cerebrospinal fluid, urines, effusions, serum leaks, joint fluid,leukorrhoea, pus and related others,b) suspensions in isotonic smear solutions, tissue punctuates, sputum, rinsings form the bronchi andrespiratory pathways and related others.These suspensions may be tested in order to:1) obtain precipitates of fine matter and the filtrate from the same sample of tested biologicalsuspension,2) obtain just a precipitate of the fine matter through passing of the filtrate into a blotting paperThe cytology kit is essential in medicine and veterinary science, and may also be used in industry as well astechnology.8.2. Benefits of “Cytoset”Fast desposition of cells on the microscope slide through centrifugation and the penetration of thefiltrate into the blotting/filter paper.Possibility of filtrate recovery after the cells have settled on the slide by its automatic collection into atest tube.Securing the fluid against its escaping into the centrifugation chamber (avoiding air-borncontamination).Inserts used as disposable equipment will help prevent infections, contamination of lab personnel or theenvironment.Possibility of having varied size (diameter) areas of cell settlement on the microscope slide.8.3. Work parameters.Rotational speedMax. accelerationMax. centrifugation volumeArea diameter of settling cells on the microscope slide500 - 2500 rpm750 x g2 ml 8.7 mm.14
8.4. Cytoset contents.The kit contains a horizontal four-pocket rotor with hangers, into which the complete cyto-container isplaced.68745321Drawing No. 1.Cyto-container.The complete insert contains the following:- base – collection chamber (1),- basic microscope slide (2),- blotting/filter card (3),- cyto-container (4),- seal 8.7 mm (60 mm 2 ),- plug (6),- plug (7),- dacantation test tube (8).8.5. Working principle.During the centrifugation, the cyto container change into a horizontal position, which positioning is thenblocked with snap fastening units located in the side arms of the rotor in case of the need to decant thefiltrate.Under the centrifugal force the morphotic elements (cell precipitate) are separated from thesuspension and settle on the microscope slide. The cell-free filtrate, depending on the method used, is eitherabsorbed by the blotting paper or decanted into a test tube following centrifugation. After the decantationtest tube containing the filtrate is removed in order to dry the precipitate on the microscope slide, the testtube is subjected to a second centrifugation.15
Page 1 and 2: centrifuge - Cat. No.:user manual -
Page 3 and 4: Contents1. Application.2. Technical
Page 7 and 8: 3.4. Fuses.The centrifuge has stand
Page 9 and 10: In order to protect the centrifuge
Page 11 and 12: 6.6. Safety devices.Apart from the
Page 13: 7.3. Mathematical relations.7.3.1.
Page 17 and 18: 8.8. Preparation of the cyto-contai
Page 19 and 20: 8.12. Procedure after centrtifugati
Page 21 and 22: Abbreviations of names of character
Page 23 and 24: 11. Work safety.11.1. Work safety i
Page 25 and 26: Tel: (+48) 22 673 04 08, 22 879 70
Page 27: DECLARATION OF DECONTAMINATIONIn or

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