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Cell precipitateCYTOFiltrateSUPERNATANTDrawing No. 2.The cyto-container, following the completion of the settling of the morphotic elements on the basic slidecentrifugation with filtrate retrieval.8.6. Preparation of samples for centrifugation.The amount of fluid used in centrifugation depends on its contents of cells, as well as its availability. If thefluid is cell-dense in order to avoid layering of the cells on the slide or their settling too densely, the fluidshould be diluted with 0.9 % NaCl solution or PBS.8.7. Tabel of dilutions in hematology.Number ofwhite cells101 - 300301 - 700701 - 15001501 - 3000Dilutionfactor-1 : 21 : 51 : 10Sampledrops201042Saline drops0101618When a cell-poor fluid with a low protein content (about 0.2 mg/ml) is tested there exists a danger, that thecentrifuged cells on the slide may be fragmented, as well as the quality of the preparation is often notsatisfactory. For that reason, if the filtrate is not needed for further tests, it is recommended that a few dropsof blood serum or albumin solutions be added. Samples destined for cyto-centrifugation should be fresh inorder to maximize the possibility of getting intact cells.16