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Microarray Data Analysis Using BRB-ArrayTools Version 4.2.0 ...

Microarray Data Analysis Using BRB-ArrayTools Version 4.2.0 ...

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Expression data• Input data as tab-delimited ASCII files (or Excelspreadsheets) in one of the following three formats:1. Horizontally aligned2. Separate files3. Multi-chip sets• Files may contain expression data in the form of signal (orsingle-channel expression summary), dual-channelintensities, or expression ratios (for dual-channel data).<strong>Data</strong> may or may not have been already log-transformed.Flags, detection call, and spot size may also be used. Allother variables will be ignored.• For Affymetrix data, expression data files should bePROBESET-level data if using the <strong>Data</strong> Import Wizard.Affymetrix CEL files should be imported using aspecialized utility included with <strong>BRB</strong>-<strong>ArrayTools</strong>.27

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