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Thermo Scientific NanoDrop SpectrophotometersNucleic AcidTroubleshootingCommon ReagentsBelow are several examples of reagents commonly used with nucleic acids that have absorbance inthe 220 – 240 nm range. Note: Phenol also exhibits significant absorbance between 260 – 270 nmwhich may shift the peak and result in an overestimation of the nucleic acid concentration.12.22 --9.38 --10mm 10mm Absorbance12.22 11.00 --8.009.38 --10.00 11.00 --9.00 7.00 8.00 --10.00 --8.00 6.00 9.00 --7.00 --7.00 8.00 --5.00 6.00 --6.00 7.00 --4.00 5.00 --5.00 6.00 --4.00 3.00 4.00 --5.00 --3.00 4.00 --2.00 3.00 --2.00 3.00 --1.00 2.00 --1.00 2.00 --0.00 0.00 1.00 --1.00 --220 230 240 250 260 270 280 290 300 310 320 330 340 350 220 230 240 250 260 270 280 290 300 310 320 330 340 3500.00 --Wavelength nm0.00 --Wavelength nm220 230 240 250 260 270 280 290 300 310 320 330 340 350 220 230 240 250 260 270 280 290 300 310 320 330 340 350Wavelength nmWavelength nmPhenol10mm 10mm AbsorbanceEDTA60 --60 --50 --12 --12 --10 --10mm 10mm Absorbance50 --40 --40 --30 --30 --20 --20 --10 --10mm 10mm Absorbance10 --8 --8 --6 --6 --4 --4 --2 --10 --2 --0 --0 --220 230 240 250 260 270 280 290 300 310 320 330 340 350 220 230 240 250 260 270 280 290 300 310 320 330 340 3500 --0 --Wavelength nmWavelength nm220 230 240 250 260 270 280 290 300 310 320 330 340 350 220 230 240 250 260 270 280 290 300 310 320 330 340 350Wavelength nmWavelength nmGuanidine IsothiocyanateGuanidine HCITip: For samples with ratios that fall outside the expected optimal values, it is suggestedthat the user refer to the manufacturer of the isolation kit for additional informationregarding protocol optimization and troubleshooting.18