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Nucleic AcidThe information in this publication is provided for reference only. All information contained in this publication is believed to be correct andcomplete. Thermo Fisher Scientific shall not be liable for errors contained herein nor for incidental or consequential damages in connectionwith the furnishing, performance or use of this material. All product specifications, as well as the information contained in this publication, aresubject to change without notice.This publication may contain or reference information and products protected by copyrights or patents and does not convey any license under our patentrights, nor the rights of others. We do not assume any liability arising out of any infringements of patents or other rights of third parties.We make no warranty of any kind with regard to this material, including but not limited to the implied warranties of merchantability and fitness for aparticular purpose. Customers are ultimately responsible for validation of their systems.© 2010 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.Copyrights in and to the technical support image are owned by a third party and licensed for limited use only to Thermo Fisher Scientific byiStockphoto. No part of this publication may be stored in a retrieval system, transmitted, or reproduced in any way, including but not limited to photocopy,photograph, magnetic or other record, without our prior written permission.Microsoft, Windows, Windows NT and Excel are either trademarks or registered trademarks of Microsoft Corporation in the United States and/orother countries.Adobe and Acrobat are trademarks of Adobe Systems, Incorporated. All other trademarks are the property of Thermo Fisher Scientific Inc. and itssubsidiaries.NanoDrop is a trademark of Thermo Fisher Scientific. Revised 11/2010
Thermo Scientific NanoDrop SpectrophotometersNucleic AcidContentsIntroduction 4Sample Retention Technology 5Best Practices 6Cleaning & Reconditioning 6Instrument & Cuvette Orientation 7Sampling Technique 8Sample Purification 9Measurements 10Blank Measurements 10Sample Measurements 10Concentration Calculations 12Modified Beer-Lambert Equation 12Pathlength & Concentration 13Purity Ratios 14Calibration 15Calibration Verification 15Standard vs Control 16Troubleshooting 17Purity Ratios 17Common Reagents 18Unusual Spectra 19Reproducibility 20Instrument Related Issues 21Installation Errors 22Connection Errors 23Signal Errors 23FAQs 24Technical Support 27
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