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Protein Engineering Protocols - Mycobacteriology research center

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Degenerate Oligonucleotide Gene Shuffling 2032. Stemmer, W. P. C. (1994) Rapid evolution of a protein in vitro by DNA shuffling.Nature 370, 389–391.3. Stemmer, W. P. C. (1994) DNA shuffling by random fragmentation and reassembly:in vitro recombination for molecular evolution. Proc. Natl. Acad. USA 91,10,747–10,751.4. Schmidt-Dannert, C., Umeno, D., and Arnold, F. H. (2000) Molecular breeding ofcarotenoid biosynthetic pathways. Nature Biotechnol. 18, 750–753.5. Kikuchi, M., Ohnishi, K., and Harayama, S. (1999) Novel family shuffling methodsfor the in vitro evolution of enzymes. Gene 236, 159–167.6. Morris, D. D., Gibbs, D. D., Chin, C. W., et al. (1998) Cloning of the xynB genefrom Dictyoglomus thermophilum strain Rt46B.1 and action of the gene-producton kraft pulp. Appl. Environ. Microbiol. 64, 1759–1765.7. Gibbs, M. D., Nevalainen, K. M. H., and Bergquist, P. L. (2001) Degenerateoligonucleotide gene shuffling (DOGS): a method for enhancing the frequency ofrecombination with family shuffling. Gene 271, 13–20.8. Sakka, K., Kojima, Y., Kondo, T., Karita, S., Ohmiya, K., and Shimada, K. (1993)Nucleotide sequence of the Clostridium stercorarium xynA gene encoding xylanaseA: identification of catalytic and cellulose binding domains. Biosci. Biotechnol.Biochem. 57, 273–277.9. Yang, V. W., Zhuang, Z., Elegir, G., and Jeffries, T. W. (1995) Alkaline-activexylanase produced by an alkaliphilic Bacillus sp isolated from kraft pulp. J. Ind.Microbiol. 15, 434–441.10. Morris, D. D., Gibbs, M. D., Ford, M., Thomas, J., and Bergquist, P. L. (1999) Family10 and 11 xylanase genes from Caldicellulosiruptor isolate Rt69B.1. Extremophiles3, 103–111.11. Fernandes, A. C., Fontes, C. M., Gilbert, H. J., Hazlewood, G. P., Fernandes, T. H.,and Ferreira, L. M. (1999) Homologous xylanases from Clostridium thermocellum:evidence for bi-functional activity, synergism between xylanase catalytic modulesand the presence of xylan-binding domains in enzyme complexes. Biochem. J. 342,105–110.12. Elegir, G., Szakacs, G., and Jeffries, T. W. (1994) Purification, characterization andsubstrate specificity of multiple xylanases from Streptomyces sp strain B-12-2.Appl. Environ. Microbiol. 60, 2609–2615.13. Rose, T. M., Schultz, E. R., Henikoff, J. G., Pietrokovski, S., McCallum, C. M., andHenikoff, S. (1998) Consensus-degenerate hybrid oligonucleotide primers foramplification of distantly related sequences. Nucleic Acids Res. 26, 1628–1635.14. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., and Higgins, D. G.(1997) The ClustalX windows interface: flexible strategies for multiple sequencealignment aided by quality analysis tools. Nucl. Acids Res. 24, 4876–4882.15. Rice, P., Longden, I., and Bleasby, A. (2000) EMBOSS: The European MolecularBiology Open Software Suite. Trends Genet. 16, 276–277.16. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K., and Pease, L. R. (1989) Sitedirectedmutagenesis by overlap extension using the polymerase chain reaction. Gene77, 51–59.

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