Protein Engineering Protocols - Mycobacteriology research center

Synthesis of Libraries and Screening With the DHFR PCA 271Dupras for help with the CAD constructs, and Joelle Pelletier and other membersof the laboratory who contributed to the development of the DHFR PCA.F.-X. C.-V. is a Canadian Institutes of Health Research and Fonds pour la formationde cherocheors et l’aide à la recherhe scholar.References1. Chen, K. Q. and Arnold, F. H. (1991) Enzyme engineering for nonaqueous solvents:random mutagenesis to enhance activity of subtilisin E in polar organicmedia. Biotechnology (NY) 9, 1073–1077.2. Chen, K. Q., Robinson, A. C., Van Dam, M. E., Martinez, P., Economou, C., andArnold, F. H. (1991) Enzyme engineering for nonaqueous solvents. II. Additiveeffects of mutations on the stability and activity of subtilisin E in polar organicmedia. Biotechnol. Prog. 7, 125–129.3. Iffland, A., Tafelmeyer, P., Saudan, C., and Johnsson, K. (2000) Directed molecularevolution of cytochrome c peroxidase. Biochemistry 39, 10,790–10,798.4. Scott, J. K. and Smith, G. P. (1990) Searching for peptide ligands with an epitopelibrary. Science 249, 386–390.5. Lowman, H. B., Bass, S. H., Simpson, N., and Wells, J. A. (1991) Selecting highaffinitybinding proteins by monovalent phage display. Biochemistry 30, 10,832–10,838.6. Keefe, A. D. and Szostak, J. W. (2001) Functional proteins from a randomsequencelibrary. Nature 410, 715–718.7. Imanaka, T., Shibazaki, M., and Takagi, M. (1986) A new way of enhancing thethermostability of proteases. Nature 324, 695–697.8. Shih, P. and Kirsch, J. F. (1995) Design and structural analysis of an engineeredthermostable chicken lysozyme. Protein. Sci. 4, 2063–2072.9. Riddle, D. S., Santiago, J. V., Bray-Hall, S. T., et al. (1997) Functional rapidly foldingproteins from simplified amino acid sequences. Nat. Struct. Biol. 4, 805–809.10. Kim, D. E., Gu, H., and Baker, D. (1998) The sequences of small proteins are notextensively optimized for rapid folding by natural selection. Proc. Natl. Acad. Sci.USA 95, 4982–4986.11. Kamtekar, S., Schiffer, J. M., Xiong, H., Babik, J. M., and Hecht, M. H. (1993)Protein design by binary patterning of polar and nonpolar amino acids. Science262, 1680–1685.12. Stemmer, W. P. (1994) Rapid evolution of a protein in vitro by DNA shuffling.Nature 370, 389–391.13. Crameri, A., Raillard, S. A., Bermudez, E., and Stemmer, W. P. (1998) DNA shufflingof a family of genes from diverse species accelerates directed evolution.Nature 391, 288–291.14. Zhao, H., Giver, L., Shao, Z., Affholter, J. A., and Arnold, F. H. (1998) Molecularevolution by staggered extension process (StEP) in vitro recombination. Nat.Biotechnol. 16, 258–261.15. Ostermeier, M., Shim, J. H., and Benkovic, S. J. (1999) A combinatorialapproach to hybrid enzymes independent of DNA homology. Nat. Biotechnol.17, 1205–1209.