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Aim: Solid-phase antibody (Ab) detection assays are among the most important tools for determiningunacceptable antigens (UA) currently used in HLA labs. We report here a comparison of Ab identificationamong the Michigan HLA labs aiming to evaluate the extent of inter-laboratory variability in the solidphaseplatform.Methods: Four sera were tested by six labs using the Luminex-based bead assays. Data collected includedtest performed, reagent vendor and lot used, Ab specificities identified and the MFI values, UA assigned,and thresholds used for reporting.Results: While strong Ab with a mean MFI of >6000 were concordantly reported as UAs by all labs, muchvariability was observed in the identification of moderate (MFI 2000-6000) and weak (MFI
Aim: There is increasing evidence that allosensitization represents an important risk factor in hearttransplantation. Progress in antibody detection and desensitization of patients with DSA has resulted inbetter patient and kidney allograft survival, yet its impact on heart transplantation has not been analyzed indepth. We have analyzed the impact of anti-HLA antibodies developed prior and/or followingtransplantation by patients from a cohort of 950 adult recipients of heart allografts performed at ourInstitution between January 1,1995 and December 31, 2009.Methods: Sera were tested for anti-HLA antibodies using the standard complement-dependentmicrolymphocytotoxicity method. Antibody specificities were assigned based on correlation coefficients≥0.70 between serum reactivity and expression of distinct HLA antigen(s) on target panel cells. Sera wereconsidered positive for anti-HLA antibodies when they reacted with more than 10% of the HLA referencepanel.Results: Allosensitization reflected in PRA ≥10% and/or in antibodies with distinct anti-HLA specificitieswas present prior to transplantation in 16% (151/950) of the patient population. The actuarial graft survivalafter 15 years was not significantly different in allo-sensitized compared to non-sensitized recipients.However, post-transplant monitoring of PRA demonstrated that there was a significant difference (p500, as determined by singleantigen bead assays, with presumably minimal interferences from the class II antibodies (class II DSA ofMFI
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Page 3 and 4: 5-ORTWO CASES OF DONOR SPECIFIC ANT
Page 5 and 6: Monday, September 27, 20102:00 PM -
Page 7 and 8: Results: PHA-induced mRNA expressio
Page 9 and 10: conjugated IgM (BD Biosciences), Ig
Page 11 and 12: Methods: Peripheral blood and endom
Page 13 and 14: Aim: NK cells have an established r
Page 15 and 16: Methods: Sera (n=22) were tested fo
Page 17 and 18: expression on lymphocytes, we hypot
Page 19 and 20: Conclusions: Clean SA beads can eli
Page 21: goal is to re-examine our previous
Page 25 and 26: esult in poor graft survival. The g
Page 27 and 28: Aim: Pronase treatment eliminates i
Page 29 and 30: 34-PCLINICAL AND PATHOLOGICAL SIGNI
Page 31 and 32: Nabil Mohsin, Isabelle G. Wood, Ind
Page 33 and 34: donor/recipient pairs for transplan
Page 35 and 36: and tested in the DynaChip assay fo
Page 37 and 38: average difference between Ave and
Page 39 and 40: Conclusions: The XM-One assay can b
Page 41 and 42: 59-PCYTOTOXIC AND NON-CYTOTOXIC ANT
Page 43 and 44: data suggests that DTT pretreatment
Page 45 and 46: Bhavna Lavingia 1 , Chantale Lacell
Page 47 and 48: ATG INTERFERNCE IN SINGLE ANTIGEN L
Page 49 and 50: 75-PPREFORMED LOW REACTIVE ANTI-HLA
Page 51 and 52: Sabarinathan Ramachandran 1 , Naohi
Page 53 and 54: 83-PALLELIC DIVERSITY OF KIR2DL4 AN
Page 55 and 56: São Paulo, Brazil; 3 Commissariat
Page 57 and 58: 91-PCHARACTERIZATION OF COMMON AND
Page 59 and 60: Conclusions: The extensive diversit
Page 61 and 62: 99-PPOTENTIAL COMMON NOVEL ALLELES
Page 63 and 64: 103-PTOWARDS A FUNCTIONAL HLA-DPB1
Page 65 and 66: 107-PWHOLE GENOME AMPLIFICATION (WG
Page 67 and 68: 111-PLOSS OF MISMATCHED HLA IN FAMI
Page 69 and 70: 115-PDONOR EPITHELIAL CELL CHIMERIS
Page 71 and 72: Caucasian patients. MRs vary signif
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Results: We obtained the total No.
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cord blood and platelet transfusion
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131-PASSOCIATION OF IL-2-330(G/T) W
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Institute, Children’s Hospital Oa
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Methods: To prove the robustness of
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populations.We studied the involvem
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amplicons generated from different
Page 87 and 88:
Results: The sequence of the unexpe
Page 89 and 90:
concentration of the Taq Polymerase
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160-PASSOCIATION OF HAPLOTYPES WITH
Page 93 and 94:
164-PDETECTION OF A DE NOVO DONOR S
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168-PA NOVEL HLA-DQB1 ALLELE CONFIR
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172-PSCREENING FOR HLA-SPECIFIC ALL
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176-PIMPLEMENTATION OF THE WORLD HE
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epertoire of displayed ligands and
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mucosa. No other samples were avail
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Conclusions: In previously reported
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Conclusions: Our findings can demon
Page 109 and 110:
Conclusions: In analyzing this data
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examined on microarrays from a huma
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Methods: Serial analysis of sera ob
Page 115 and 116:
Conclusions: These new technologies
Page 117 and 118:
allograft injury. Four of these 18
Page 119 and 120:
A NOVEL HLA CLASS I SINGLE ANTIGEN
Page 121 and 122:
Results: Approximately twice as man
Page 123:
Methods: Molecular HLA typing was p
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